[go: up one dir, main page]

AR003776A1 - Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto. - Google Patents

Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto.

Info

Publication number
AR003776A1
AR003776A1 ARP960104582A ARP960104582A AR003776A1 AR 003776 A1 AR003776 A1 AR 003776A1 AR P960104582 A ARP960104582 A AR P960104582A AR P960104582 A ARP960104582 A AR P960104582A AR 003776 A1 AR003776 A1 AR 003776A1
Authority
AR
Argentina
Prior art keywords
jet device
rupture
cells
impact jet
cultivated cells
Prior art date
Application number
ARP960104582A
Other languages
English (en)
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9603759.3A external-priority patent/GB9603759D0/en
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of AR003776A1 publication Critical patent/AR003776A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16751—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Sustainable Development (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un novedoso método para fracturar células que no tienen pared celular comprende pasar células suspendidas a través de un dispositivo de chorros deimpacto a baja presión. Este método fractura las células, pero no dana los productos celulares liberados.
ARP960104582A 1995-10-06 1996-10-02 Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto. AR003776A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US506295P 1995-10-06 1995-10-06
GBGB9603759.3A GB9603759D0 (en) 1996-02-22 1996-02-22 Method of disrupting cultured cells using an impinging jet device

Publications (1)

Publication Number Publication Date
AR003776A1 true AR003776A1 (es) 1998-09-09

Family

ID=26308786

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP960104582A AR003776A1 (es) 1995-10-06 1996-10-02 Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto.

Country Status (10)

Country Link
EP (1) EP0854913A1 (es)
CN (1) CN1100868C (es)
AR (1) AR003776A1 (es)
AU (1) AU7204496A (es)
BR (1) BR9610773A (es)
CZ (1) CZ104198A3 (es)
EA (1) EA000703B1 (es)
SK (1) SK43498A3 (es)
TW (1) TW426733B (es)
WO (1) WO1997012959A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19749735C1 (de) * 1997-11-11 2000-02-10 Invent Gmbh Entwicklung Neuer Technologien Verfahren und Vorrichtung zur Herstellung von Enzymen
NZ533586A (en) 2001-12-20 2005-02-25 Bavarian Nordic As Method for the recovery and purification of poxviruses from infected cells using high pressure homogenisation
DE102005034629B4 (de) * 2005-07-19 2007-09-13 Dr. Hielscher Gmbh Vorrichtung und Verfahren zum mechanischen Aufschluss von Zellen
CN101522884B (zh) * 2006-08-15 2013-08-21 巴斯夫欧洲公司 从生产细胞中分离蛋白质的方法
JP6001456B2 (ja) * 2010-12-28 2016-10-05 中外製薬株式会社 動物細胞の培養方法
CN104861038B (zh) * 2015-05-08 2019-10-01 永联生物科技(上海)有限公司 一种均质阀组
BR102018004973B1 (pt) * 2018-03-13 2021-10-13 Petróleo Brasileiro S.A. - Petrobras Dispositivo e método para rompimento de células de microorganismos por extrusão
EP4296352A1 (en) 2022-06-20 2023-12-27 LenioBio GmbH Tunable disruption of eukaryotic protoplast to release intact cellular organelles

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR346862A (fr) * 1904-10-07 1905-02-13 Henri Charles Empis Extraction et préparation des substances contenues dans les cellules organiques
GB877898A (en) * 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
US3309032A (en) * 1964-03-23 1967-03-14 Sorvall Inc Ivan Cell fractionator apparatus
DE3810462A1 (de) * 1988-03-26 1989-10-05 Dechema Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens

Also Published As

Publication number Publication date
AU7204496A (en) 1997-04-28
TW426733B (en) 2001-03-21
EA000703B1 (ru) 2000-02-28
CZ104198A3 (cs) 1998-08-12
EP0854913A1 (en) 1998-07-29
CN1203626A (zh) 1998-12-30
CN1100868C (zh) 2003-02-05
WO1997012959A1 (en) 1997-04-10
SK43498A3 (en) 1998-12-02
EA199800266A1 (ru) 1998-10-29
BR9610773A (pt) 1999-07-13

Similar Documents

Publication Publication Date Title
ATE367768T1 (de) Gerät zur reduzierung der spannung der herzwand
ES2196054T3 (es) Derivados de acido fenilacetico, procedimiento y productos intermedios para su obtencion y agentes que contienen los mismos.
DK0828717T3 (da) Fremgangsmåde til fremstilling af 3-haloalkyl-1H-pyrazol
AR003776A1 (es) Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto.
GT199900102A (es) Fenoxipropanolaminas, procedimiento para su preparacìon y composiciones farmacèuticasque las contienen.
MX9305122A (es) Metodo y aparato para manipular y realizar una transferencia en un sistema de comunicacion inalambrio.
ES2121532B1 (es) Sistema de alarma visual/audible sincronizado.
BR9814119A (pt) Método para localizar uma estação móvel utilizando processamento distribuìdo
ES2042737T3 (es) Un procedimiento para la preparacion de un glucosido de antraciclina.
ES2090217T3 (es) Laca fotosensible.
BR0208194A (pt) Método e aparelho para corte de estruturas submarinas
ES2176519T3 (es) Procedimiento de seguimiento para sistema de radar.
MX9300140A (es) Dispositivo para la deteccion de hemolisis de globulos rojos en la sangre en una via sanguinea extracorporal y metodo para la misma
AR245684A1 (es) Procedimiento mejorado de hidrogenolisis/deshidrohalogenacion
ES2161952T3 (es) Procedimiento y dispositivo para atenuar una detonacion en un sistema de recipiente o de tuberias.
ES2144748T3 (es) Dispositivo y procedimiento para la produccion de material estirado.
ES2096484T3 (es) Bloques constitutivos con enlaces de carbamato entre nucleosidos y nuevos oligonucleotidos derivados de los mismos.
AR017013A1 (es) Un metodo para ajustar una estacion de comunicaciones personales en un modo de operacion seleccionado y una estacion de comunicaciones personales parallevar a la practica dicho metodo.
ES2044542T3 (es) Util de corte y procedimiento para su fabricacion.
NO980864L (no) Fremgangsmåte og krets for tenning av en höytrykksgassutladningslampe
ES2106623T3 (es) Nuevo procedimiento de preparacion de un esteroide 16betametilo y nuevos productos intermedios.
ES2177825T3 (es) Procedimiento para la proteccion selectiva de los derivados de la baccatina y su utilizacion en la sintesis de taxanos.
IT1144129B (it) Perforatore iniettore per interventi attraverso pareti resistenti particolarmente per la forza pubblica e per pompieri
DE59608096D1 (de) Vorrichtung zur Druckverteilung im Kopfstück einer Apparatur zum ballistischen Transfer von Zellen
DE60000479D1 (de) Vorrichtung zum kontrolierten trennen