EA004942B1 - Композиции и способы для осуществления реакций галогенирования - Google Patents

Композиции и способы для осуществления реакций галогенирования Download PDF

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Publication number: EA004942B1
Authority: EA; Eurasian Patent Office
Prior art keywords: plant; reductase; halogenase; host cell; nucleic acid
Prior art date: 1999-12-15

Application number

EA200200634A

Other languages

English (en)

Russian (ru)

Other versions

EA200200634A1 (ru

Inventor

Джон Стеффенс

Крис Бейти

Джон Маркуис Диц

Янь Дон

Ким Пулома Камдар

Стив Хилл

Original Assignee

Зингента Партисипейшнс Аг

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-12-15

Filing date

2000-12-07

Publication date

2004-10-28

2000-12-07 Application filed by Зингента Партисипейшнс Аг filed Critical Зингента Партисипейшнс Аг

2002-12-26 Publication of EA200200634A1 publication Critical patent/EA200200634A1/ru

2004-10-28 Publication of EA004942B1 publication Critical patent/EA004942B1/ru

Links

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239000007787 solid Substances 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
230000007928 solubilization Effects 0.000 description 1
229960000268 spectinomycin Drugs 0.000 description 1
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
235000021012 strawberries Nutrition 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
230000004960 subcellular localization Effects 0.000 description 1
DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
239000010414 supernatant solution Substances 0.000 description 1
238000001308 synthesis method Methods 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
238000012360 testing method Methods 0.000 description 1
238000010257 thawing Methods 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
108010071964 tryptophan halogenase Proteins 0.000 description 1
238000002604 ultrasonography Methods 0.000 description 1
238000003828 vacuum filtration Methods 0.000 description 1
MYPYJXKWCTUITO-LYRMYLQWSA-O vancomycin(1+) Chemical class O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C([O-])=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)[NH2+]C)[C@H]1C[C@](C)([NH3+])[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-O 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
229940124024 weight reducing agent Drugs 0.000 description 1
229940075420 xanthine Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Plant Pathology (AREA)
Agronomy & Crop Science (AREA)
Virology (AREA)
Physics & Mathematics (AREA)
Environmental Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Pest Control & Pesticides (AREA)
Cell Biology (AREA)
Dentistry (AREA)
Nutrition Science (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Catching Or Destruction (AREA)

EA200200634A 1999-12-15 2000-12-07 Композиции и способы для осуществления реакций галогенирования EA004942B1 (ru)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US22880199P	1999-12-15	1999-12-15
US21934300P	2000-01-03	2000-01-03
PCT/EP2000/012347 WO2001044447A1 (en)	1999-12-15	2000-12-07	Compositions and methods for halogenation reactions

Publications (2)

Publication Number	Publication Date
EA200200634A1 EA200200634A1 (ru)	2002-12-26
EA004942B1 true EA004942B1 (ru)	2004-10-28

Family

ID=26913801

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EA200200634A EA004942B1 (ru)	1999-12-15	2000-12-07	Композиции и способы для осуществления реакций галогенирования

Country Status (12)

Country	Link
EP (1)	EP1238062A1 (es)
JP (1)	JP2003516749A (es)
CN (1)	CN1409759A (es)
AR (1)	AR026939A1 (es)
AU (1)	AU772124B2 (es)
BR (1)	BR0017024A (es)
CA (1)	CA2393910A1 (es)
EA (1)	EA004942B1 (es)
HU (1)	HUP0203807A3 (es)
IL (1)	IL150084A0 (es)
MX (1)	MXPA02005868A (es)
WO (1)	WO2001044447A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK1826267T3 (da)	2006-02-22	2012-07-23	Brain Biotechnology Res & Information Network Ag	Ny halogenase
CN106190942B (zh) *	2016-07-26	2019-11-26	江南大学	一种通过敲除黄素还原酶提高l-精氨酸产量的方法
EP4208445A4 (en) *	2020-09-01	2024-10-02	Enveric Biosciences Canada Inc.	Halogenated psilocybin derivatives and methods of using
WO2023224560A1 (en) *	2022-05-20	2023-11-23	Agency For Science, Technology And Research	Enzymes and uses in biocatalytic halogenation of n-heteroaryls thereof

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US5639949A (en) *	1990-08-20	1997-06-17	Ciba-Geigy Corporation	Genes for the synthesis of antipathogenic substances
DE19533072C1 (de) *	1995-09-07	1997-04-24	Fraunhofer Ges Forschung	Nachweissystem und Verfahren für die qualitative und quantitative Bestimmung von Wasserstoffperoxid, Substraten, aus denen unter der Einwirkung von Oxidasen Wasserstoffperoxid gebildet wird, und Halogeniden
US5955348A (en) *	1997-11-25	1999-09-21	Novartis Ag	Genetically modified pseudomonas strains with enhanced biocontrol activity

2000
- 2000-12-07 HU HU0203807A patent/HUP0203807A3/hu unknown
- 2000-12-07 AU AU17078/01A patent/AU772124B2/en not_active Ceased
- 2000-12-07 MX MXPA02005868A patent/MXPA02005868A/es unknown
- 2000-12-07 CA CA002393910A patent/CA2393910A1/en not_active Abandoned
- 2000-12-07 BR BR0017024-0A patent/BR0017024A/pt not_active IP Right Cessation
- 2000-12-07 JP JP2001545525A patent/JP2003516749A/ja active Pending
- 2000-12-07 CN CN00817150A patent/CN1409759A/zh active Pending
- 2000-12-07 EP EP00979674A patent/EP1238062A1/en not_active Withdrawn
- 2000-12-07 WO PCT/EP2000/012347 patent/WO2001044447A1/en not_active Ceased
- 2000-12-07 EA EA200200634A patent/EA004942B1/ru not_active IP Right Cessation
- 2000-12-07 IL IL15008400A patent/IL150084A0/xx unknown
- 2000-12-13 AR ARP000106609A patent/AR026939A1/es unknown

Also Published As

Publication number	Publication date
JP2003516749A (ja)	2003-05-20
AR026939A1 (es)	2003-03-05
HUP0203807A3 (en)	2004-10-28
EP1238062A1 (en)	2002-09-11
HUP0203807A2 (hu)	2003-02-28
CN1409759A (zh)	2003-04-09
MXPA02005868A (es)	2002-10-23
CA2393910A1 (en)	2001-06-21
BR0017024A (pt)	2003-01-07
EA200200634A1 (ru)	2002-12-26
WO2001044447A1 (en)	2001-06-21
AU772124B2 (en)	2004-04-08
AU1707801A (en)	2001-06-25
IL150084A0 (en)	2002-12-01

Publication	Publication Date	Title
US5767361A (en)	1998-06-16	Imidazolinone resistant AHAS mutants
AU720006B2 (en)	2000-05-18	Process for selection of transgenic plant cells
JP2004254705A (ja)	2004-09-16	マンノースまたはキシロースに基づく陽性選択
JPH09500528A (ja)	1997-01-21	昆虫の抑制方法
JPH10501967A (ja)	1998-02-24	澱粉含有量変性植物
Hasnain et al.	2013	Identification and characterization of the missing pyrimidine reductase in the plant riboflavin biosynthesis pathway
US6245967B1 (en)	2001-06-12	Process and DNA molecules for increasing the photosynthesis rate in plants
JP2001509376A (ja)	2001-07-24	フィードバック不感性トレオニンデヒドラターゼ／デアミナーゼを発現する植物および微生物を製造するための方法および組成物
JP2007520228A5 (es)	2008-03-13
US20030126630A1 (en)	2003-07-03	Plant sterol reductases and uses thereof
EA004942B1 (ru)	2004-10-28	Композиции и способы для осуществления реакций галогенирования
WO1997048793A9 (en)	1998-03-19	Plant sterol reductases and uses thereof
US12473565B2 (en)	2025-11-18	Glycine regulatory elements and uses thereof
AU2017370528B2 (en)	2024-01-11	Methods for improving transformation frequency
US5474929A (en)	1995-12-12	Selectable/reporter gene for use during genetic engineering of plants and plant cells
WO2008029942A1 (fr)	2008-03-13	Utilisation du gène de l'enzyme de biosynthèse de la cytokinine activée
AU720418B2 (en)	2000-06-01	Modified plants and plant products
AU2023200524A1 (en)	2023-03-02	Plant promoter and 3'utr for transgene expression
JP2003511049A (ja)	2003-03-25	プロトポルフィリノーゲンオキシダーゼ遺伝子を利用した作物の収量またはバイオマスの増大方法
US20030167513A1 (en)	2003-09-04	Selection and use of isopropylmalate synthase (IPMS) mutants desensitized in L-leucine negative feedback control
KR102736922B1 (ko)	2024-11-29	식물체의 염기전환용 재조합 벡터 및 이의 용도
JP2002527039A (ja)	2002-08-27	Ａｍｐデアミナーゼ
Sugita et al.	2025	Transcriptomic and enzymological evidence for plastid peptidoglycan synthesis in the gymnosperm Picea abies
AU2016340893A1 (en)	2018-04-26	Plant promoter for transgene expression
RU2127759C1 (ru)	1999-03-20	Способ идентификации или селекции эукариотических клеток

Legal Events

Date	Code	Title	Description
2006-10-27	MM4A	Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)	Designated state(s): AM AZ BY KZ KG MD TJ TM RU