DK136184D0 - Fremgangsmaade til udtrykkelse af et gen,der koder for prochymosin,i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaaden - Google Patents
Fremgangsmaade til udtrykkelse af et gen,der koder for prochymosin,i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaadenInfo
- Publication number
- DK136184D0 DK136184D0 DK1361/84A DK136184A DK136184D0 DK 136184 D0 DK136184 D0 DK 136184D0 DK 1361/84 A DK1361/84 A DK 1361/84A DK 136184 A DK136184 A DK 136184A DK 136184 D0 DK136184 D0 DK 136184D0
- Authority
- DK
- Denmark
- Prior art keywords
- procedure
- prochymosin
- expressing
- host cells
- expression plasmid
- Prior art date
Links
- 239000013613 expression plasmid Substances 0.000 title 1
- 108010064037 prorennin Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
- C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58038439A JPS59166086A (ja) | 1983-03-09 | 1983-03-09 | 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK136184D0 true DK136184D0 (da) | 1984-02-29 |
| DK136184A DK136184A (da) | 1984-09-10 |
Family
ID=12525334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK136184A DK136184A (da) | 1983-03-09 | 1984-02-29 | Fremgangsmaade til udtrykkelse af et gen, der koder for prochymosin, i e.coli-vaertsceller og udtrykkelsesplasmid til brug ved fremgangsmaaden |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0121775A1 (da) |
| JP (1) | JPS59166086A (da) |
| AR (1) | AR241795A1 (da) |
| AU (1) | AU572827B2 (da) |
| BR (1) | BR8401049A (da) |
| DK (1) | DK136184A (da) |
| FI (1) | FI86886C (da) |
| IE (1) | IE57264B1 (da) |
| NZ (1) | NZ207414A (da) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO841276L (no) * | 1983-03-31 | 1984-10-01 | Codon Genetic Eng Lab | Rekombinant dna-koding for et polypeptid som utviser melkesammenloepende aktivitet |
| US4721673A (en) * | 1983-09-01 | 1988-01-26 | Genex Corporation | Recovery and activation process for microbially produced calf prochymosin |
| JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
| JPS60188077A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド |
| US5082775A (en) * | 1984-05-11 | 1992-01-21 | Berlex Laboratories, Inc. | Efficient process for isolating insoluble heterologous protein using non-ionic detergents |
| US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
| GB2200118A (en) * | 1987-01-23 | 1988-07-27 | Allelix Inc | Synthetic chymosin-and prochymosin-encoding DNA segments |
| DK0574402T3 (da) | 1990-11-26 | 1998-05-18 | Chiron Corp | Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf |
| US5629167A (en) | 1994-04-19 | 1997-05-13 | Biocine S.P.A. | Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay |
| WO1999024578A2 (en) | 1997-11-06 | 1999-05-20 | Chiron S.P.A. | Neisserial antigens |
| CN1224708C (zh) | 1998-01-14 | 2005-10-26 | 启龙股份公司 | 脑膜炎奈瑟氏球菌抗原 |
| GB9808932D0 (en) | 1998-04-27 | 1998-06-24 | Chiron Spa | Polyepitope carrier protein |
| EP2261350A3 (en) | 1998-05-01 | 2012-01-04 | Novartis Vaccines and Diagnostics, Inc. | Neisseria meningitidis antigens and compositions |
| NZ530640A (en) | 1999-04-30 | 2006-06-30 | Chiron S | Conserved neisserial antigens |
| GB9911683D0 (en) | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
| GB9916529D0 (en) | 1999-07-14 | 1999-09-15 | Chiron Spa | Antigenic peptides |
| PT2275552E (pt) | 1999-10-29 | 2015-12-07 | Glaxosmithkline Biolog Sa | Péptidos antigénicos de neisseria |
| BR0107679A (pt) | 2000-01-17 | 2004-07-06 | Chiron Spa | Vacina de vesìcula de membrana externa (omv) compreendendo proteìnas de membrana externa do grupo sérico b de neisseria meningitidis |
| ATE439372T1 (de) | 2000-10-27 | 2009-08-15 | Novartis Vaccines & Diagnostic | Nukleinsäuren und proteine von gruppen a und b- streptokokken |
| WO2002036752A2 (en) | 2000-11-06 | 2002-05-10 | Chr. Hansen A/S | Method of producing non-bovine chymosin and use thereof |
| GB0107658D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
| GB0107661D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Staphylococcus aureus |
| PT2248822T (pt) | 2001-07-27 | 2017-02-14 | Glaxosmithkline Biologicals Sa | Adesinas de meningococos |
| AU2002366592B2 (en) | 2001-12-12 | 2008-01-10 | Novartis Vaccines And Diagnostics S.R.L. | Immunisation against Chlamydia trachomatis |
| CA2485695A1 (en) | 2002-05-24 | 2003-12-04 | Fred W. Wagner | Method for universal enzymatic production of bioactive peptides |
| WO2003099854A2 (en) | 2002-05-24 | 2003-12-04 | Nps Allelix Corp. | Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides |
| EP2279746B1 (en) | 2002-11-15 | 2013-10-02 | Novartis Vaccines and Diagnostics S.r.l. | Surface proteins in neisseria meningitidis |
| GB0227346D0 (en) | 2002-11-22 | 2002-12-31 | Chiron Spa | 741 |
| GB0308198D0 (en) | 2003-04-09 | 2003-05-14 | Chiron Srl | ADP-ribosylating bacterial toxin |
| AU2004235346A1 (en) | 2003-04-25 | 2004-11-11 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore |
| DE602004032144D1 (de) | 2003-06-23 | 2011-05-19 | Univ North Carolina State | Für fructooligosaccharid-nutzende verbindungen codierende nukleinsäuren aus lactobacillus acidophilus und verwendungen davon |
| CA2546319A1 (en) | 2003-11-21 | 2005-07-28 | Nps Allelix Corp. | Production of glucagon like peptide 2 and analogs |
| NZ548255A (en) | 2004-02-02 | 2010-10-29 | Ambrx Inc | Modified human interferon polypeptides and their uses |
| US7459289B2 (en) | 2004-03-08 | 2008-12-02 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor |
| CN102603895B (zh) | 2004-06-18 | 2016-09-28 | Ambrx公司 | 新颖抗原结合多肽和其用途 |
| BRPI0512396A (pt) | 2004-07-21 | 2008-03-11 | Ambrx Inc | polipeptìdeos biossintéticos utilizando aminoácidos codificados não naturalmente |
| NZ555308A (en) | 2004-12-22 | 2010-10-29 | Ambrx Inc | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides |
| KR20070090023A (ko) | 2004-12-22 | 2007-09-04 | 암브룩스, 인코포레이티드 | 변형 인간 성장 호르몬 |
| CN102212543A (zh) | 2005-04-15 | 2011-10-12 | 北卡罗来纳州大学 | 调节细菌的粘附和应激耐受力的方法和组合物 |
| ES2547554T3 (es) | 2005-11-16 | 2015-10-07 | Ambrx, Inc. | Métodos y composiciones que comprenden aminoácidos no naturales |
| GB0524066D0 (en) | 2005-11-25 | 2006-01-04 | Chiron Srl | 741 ii |
| WO2009030978A2 (en) | 2006-06-09 | 2009-03-12 | Novartis Ag | Conformers of bacterial adhesins |
| MX2009002523A (es) | 2006-09-08 | 2009-03-20 | Ambrx Inc | Polipeptido de plasma humano modificado o andamios fc y sus usos. |
| AU2008232937B2 (en) | 2007-03-30 | 2012-09-27 | Ambrx, Inc. | Modified FGF-21 polypeptides and their uses |
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| NZ586947A (en) | 2008-02-08 | 2012-11-30 | Ambrx Inc | Modified leptin polypeptides and their uses |
| BRPI0907843A2 (pt) | 2008-02-21 | 2015-08-04 | Novartis Ag | Polipeptídeos de fhbp menigocócicos |
| UA103774C2 (uk) | 2008-07-23 | 2013-11-25 | Амбркс, Інк. | Модифіковані поліпептиди бичачого гранулоцитарного колонієстимулювального фактора (g-csf) та їх застосування |
| BR122012024318A2 (pt) | 2008-09-26 | 2019-07-30 | Ambrx, Inc. | Polipeptídeos modificados de eritropoetina animal e seus usos |
| MX357314B (es) | 2008-09-26 | 2018-07-04 | Ambrx Inc | Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales. |
| CN102596240B (zh) | 2009-08-27 | 2015-02-04 | 诺华股份有限公司 | 包括脑膜炎球菌fHBP序列的杂交多肽 |
| BR112012010531A2 (pt) | 2009-10-27 | 2019-09-24 | Novartis Ag | "polipeptídeos de modificação meningocócica fhbp" |
| EP2805965A1 (en) | 2009-12-21 | 2014-11-26 | Ambrx, Inc. | Modified porcine somatotropin polypeptides and their uses |
| JP2013515080A (ja) | 2009-12-21 | 2013-05-02 | アンブルックス，インコーポレイテッド | 修飾されているウシのソマトトロピンポリペプチドおよびそれらの使用 |
| WO2011146715A1 (en) | 2010-05-19 | 2011-11-24 | North Carolina State University | Compositions and methods for the delivery of therapeutic peptides |
| US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
| SMT201900495T1 (it) | 2010-08-17 | 2019-11-13 | Ambrx Inc | Polipeptidi di relaxina modificati e loro usi |
| TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
| RU2473683C1 (ru) * | 2011-12-15 | 2013-01-27 | Общество С Ограниченной Ответственностью "Фармапарк" | СПОСОБ ПРОМЫШЛЕННОГО КУЛЬТИВИРОВАНИЯ ШТАММОВ E.coli, ПОЛУЧЕННЫХ НА ОСНОВЕ ШТАММА BL21(DE3), НЕСУЩЕГО ГЕН T7 RNA ПОЛИМЕРАЗЫ ПОД КОНТРОЛЕМ lacUV5 ПРОМОТОРА, С ПОВЫШЕННЫМ СИНТЕЗОМ БИОМАССЫ И ВЫХОДОМ ЦЕЛЕВОГО БЕЛКА В ТЕЛЬЦАХ ВКЛЮЧЕНИЯ |
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| US10590426B2 (en) | 2016-08-22 | 2020-03-17 | Board Of Trustees Of Michigan State University | Genetic control of cell size |
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| TW201837051A (zh) | 2017-02-08 | 2018-10-16 | 美商必治妥美雅史谷比公司 | 包含藥物動力學增強劑之經修飾之鬆弛素(relaxin)多肽及其用途 |
| CN107881162A (zh) * | 2017-12-25 | 2018-04-06 | 中诺生物科技发展江苏有限公司 | 一种工业化生产凝乳酶的方法 |
| IL258467A (en) | 2018-03-29 | 2018-07-04 | Ilana Kolodkin Gal | Methods of disrupting a biofilm and/or preventing formation of same |
| AU2019337610A1 (en) | 2018-09-11 | 2021-05-06 | Ambrx, Inc. | Interleukin-2 polypeptide conjugates and their uses |
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| EP4117732A1 (en) | 2020-03-11 | 2023-01-18 | Ambrx, Inc. | Interleukin-2 polypeptide conjugates and methods of use thereof |
| EP4228656A4 (en) | 2020-08-18 | 2024-09-04 | Yeda Research and Development Co. Ltd | ANTI-VIRAL AND ANTI-TUMOR COMPOUNDS |
| IL288680A (en) | 2021-12-05 | 2023-07-01 | Yeda Res & Dev | Genetically modified phages and their use |
| WO2025215636A1 (en) | 2024-04-08 | 2025-10-16 | Yeda Research And Development Co. Ltd. | Anti-defense system polypeptides and uses thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4666847A (en) * | 1981-01-16 | 1987-05-19 | Collaborative Research, Inc. | Recombinant DNA means and method |
| JPS589687A (ja) * | 1981-06-17 | 1983-01-20 | セルテク リミテッド | ポリペプチドの生産方法 |
| IE53517B1 (en) * | 1981-06-17 | 1988-12-07 | Celltech Ltd | Process for the production of a polypeptide |
| JPS5832896A (ja) * | 1981-08-24 | 1983-02-25 | Teruhiko Beppu | 複合プラスミド及びそれを含有する微生物 |
| BR8205954A (pt) * | 1981-10-14 | 1983-09-13 | Unilever Nv | Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos |
| ZA834095B (en) * | 1982-07-01 | 1984-02-29 | Genex Corp | Bovine calf chymosin |
| IE56372B1 (en) * | 1982-12-22 | 1991-07-03 | Genentech Inc | Microbially produced rennet methods for its production and plasmids used for its production |
-
1983
- 1983-03-09 JP JP58038439A patent/JPS59166086A/ja active Granted
-
1984
- 1984-02-29 DK DK136184A patent/DK136184A/da not_active Application Discontinuation
- 1984-03-07 FI FI840918A patent/FI86886C/fi not_active IP Right Cessation
- 1984-03-07 AU AU25357/84A patent/AU572827B2/en not_active Ceased
- 1984-03-07 EP EP84102451A patent/EP0121775A1/en not_active Withdrawn
- 1984-03-07 NZ NZ207414A patent/NZ207414A/en unknown
- 1984-03-08 IE IE559/84A patent/IE57264B1/en not_active IP Right Cessation
- 1984-03-08 BR BR8401049A patent/BR8401049A/pt unknown
- 1984-03-09 AR AR84295974A patent/AR241795A1/es active
Also Published As
| Publication number | Publication date |
|---|---|
| AU572827B2 (en) | 1988-05-19 |
| NZ207414A (en) | 1988-02-29 |
| FI86886C (fi) | 1992-10-26 |
| IE840559L (en) | 1984-09-09 |
| IE57264B1 (en) | 1992-07-01 |
| EP0121775A1 (en) | 1984-10-17 |
| JPS6240999B2 (da) | 1987-09-01 |
| DK136184A (da) | 1984-09-10 |
| BR8401049A (pt) | 1984-10-16 |
| AR241795A1 (es) | 1992-12-30 |
| JPS59166086A (ja) | 1984-09-19 |
| AU2535784A (en) | 1984-09-13 |
| FI840918A0 (fi) | 1984-03-07 |
| FI86886B (fi) | 1992-07-15 |
| FI840918L (fi) | 1984-09-10 |
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