DK0461165T3 - Gærspaltningssystem - Google Patents

Gærspaltningssystem

Info

Publication number: DK0461165T3
Authority: DK; Denmark
Prior art keywords: terminal end; polypeptide; heterologous protein; yeast; presentation
Prior art date: 1989-03-03

Application number

DK90904258.2T

Other languages

English (en)

Inventor

Kjeld Norris

Fanny Norris

Soren Bjorn

Original Assignee

Novo Nordisk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-03-03

Filing date

1990-03-01

Publication date

1994-11-21

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26065137&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0461165(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1989-10-06 Priority claimed from DK494189A external-priority patent/DK494189D0/da

1990-03-01 Application filed by Novo Nordisk As filed Critical Novo Nordisk As

1994-11-21 Application granted granted Critical

1994-11-21 Publication of DK0461165T3 publication Critical patent/DK0461165T3/da

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
238000003776 cleavage reaction Methods 0.000 title 1
230000007017 scission Effects 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 4
102000004169 proteins and genes Human genes 0.000 abstract 4
108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
229920001184 polypeptide Polymers 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
150000001413 amino acids Chemical group 0.000 abstract 2
210000004899 c-terminal region Anatomy 0.000 abstract 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
108010039627 Aprotinin Proteins 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
229960004405 aprotinin Drugs 0.000 abstract 1
230000004927 fusion Effects 0.000 abstract 1
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
239000002243 precursor Substances 0.000 abstract 1
230000006337 proteolytic cleavage Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Mycology (AREA)
Diabetes (AREA)
Toxicology (AREA)
Endocrinology (AREA)
Botany (AREA)
Tropical Medicine & Parasitology (AREA)
Virology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Processing Of Solid Wastes (AREA)
Chemical Or Physical Treatment Of Fibers (AREA)
Silver Salt Photography Or Processing Solution Therefor (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
Thermotherapy And Cooling Therapy Devices (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Particle Accelerators (AREA)

DK90904258.2T 1989-03-03 1990-03-01 Gærspaltningssystem DK0461165T3 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DK105489A DK105489D0 (da)	1989-03-03	1989-03-03	Polypeptid
DK494189A DK494189D0 (da)	1989-10-06	1989-10-06	Polypeptid

Publications (1)

Publication Number	Publication Date
DK0461165T3 true DK0461165T3 (da)	1994-11-21

Family

ID=26065137

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
DK105489A DK105489D0 (da)	1989-01-06	1989-03-03	Polypeptid
DK90904258.2T DK0461165T3 (da)	1989-03-03	1990-03-01	Gærspaltningssystem

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
DK105489A DK105489D0 (da)	1989-01-06	1989-03-03	Polypeptid

Country Status (23)

Country	Link
US (3)	US5395922A (da)
EP (1)	EP0461165B2 (da)
JP (1)	JP2609367B2 (da)
KR (1)	KR970005928B1 (da)
AT (1)	ATE110414T1 (da)
AU (1)	AU624694B2 (da)
CA (1)	CA2050336C (da)
CZ (1)	CZ285239B6 (da)
DD (1)	DD296965A5 (da)
DE (1)	DE69011853T3 (da)
DK (2)	DK105489D0 (da)
ES (1)	ES2062514T5 (da)
FI (1)	FI104985B (da)
HU (2)	HU215538B (da)
IE (1)	IE66469B1 (da)
IL (1)	IL93609A (da)
NO (2)	NO304236B1 (da)
NZ (1)	NZ232742A (da)
PL (1)	PL163532B1 (da)
RU (1)	RU2194758C2 (da)
UA (1)	UA27706C2 (da)
WO (1)	WO1990010075A1 (da)
ZA (1)	ZA901476B (da)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
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US5591603A (en) *	1987-08-28	1997-01-07	Novo Nordisk A/S	Process for preparing aprotinin and aprotinin analogs in yeast cells
DK105489D0 (da) *	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid
WO1990014431A1 (en) *	1989-05-19	1990-11-29	Biotechnology Research And Development Corporation	Fusion proteins having an in vivo post-translational modification site and methods of manufacture and purification
GB9015825D0 (en) *	1990-07-18	1990-09-05	Ciba Geigy Ag	In vitro processing of fusion proteins
DK300090D0 (da) *	1990-12-19	1990-12-19	Novo Nordisk As	Fremgangsmaade til fremstilling af leadersekvenser
GB9106185D0 (en) *	1991-03-22	1991-05-08	Wellcome Found	Biological control agents
FR2686899B1 (fr) *	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FI92601C (fi) *	1992-03-11	1994-12-12	Marja Makarow	Menetelmä hyötyproteiinien erittämiseksi hiivoista
US5521086A (en) *	1993-09-16	1996-05-28	Cephalon, Inc.	Secretion sequence for the production of a heterologous protein in yeast
DE4417353A1 (de) *	1994-05-18	1996-01-25	Bayer Ag	Verfahren zur Herstellung von rekombinantem Aprotinin und rekombinanten Aprotinin Varianten mit der natürlichen N-terminlaen Sequenz
US6500645B1 (en)	1994-06-17	2002-12-31	Novo Nordisk A/S	N-terminally extended proteins expressed in yeast
IL114160A (en) *	1994-06-17	2006-12-31	Novo Nordisk As	Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
US5861267A (en) *	1995-05-01	1999-01-19	Vertex Pharmaceuticals Incorporated	Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
ZA9610456B (en) *	1995-12-20	1997-06-20	Novo Nordisk As	N-terminally extended proteins expressed in yeast
US5851800A (en) *	1996-05-14	1998-12-22	Pharmacia & Upjohn Ab	Process for producing a protein
DE19629982A1 (de)	1996-07-25	1998-01-29	Bayer Ag	Aprotinin-Varianten mit verbesserten Eigenschaften
DE69739319D1 (de)	1996-12-13	2009-04-30	Novartis Vaccines & Diagnostic	Verfahren zur Expression von PDGF oder IGR Proteinen in Hefe
ATE317443T1 (de) *	1996-12-20	2006-02-15	Novo Nordisk As	N-terminal verlängerte proteine exprimiert in hefe
EP0954589A1 (en) *	1997-01-24	1999-11-10	Novo Nordisk A/S	Synthetic leader peptide sequences
US6046000A (en) *	1997-11-07	2000-04-04	Millennium Biotherapeutics, Inc.	Method for identifying genes encoding signal sequences
EP1049790A1 (en)	1998-01-23	2000-11-08	Novo Nordisk A/S	Process for making desired polypeptides in yeast
JP4795599B2 (ja)	1999-12-29	2011-10-19	ノボノルディスクアクティーゼルスカブ	酵母において改良された発酵収率を有するインスリン前駆体及びインスリン前駆体類似体の製造方法
US20050100991A1 (en) *	2001-04-12	2005-05-12	Human Genome Sciences, Inc.	Albumin fusion proteins
DK2236152T3 (da)	2000-04-12	2014-07-07	Novozymes Biopharma Dk As	Albuminfusionsproteiner
US7507413B2 (en) *	2001-04-12	2009-03-24	Human Genome Sciences, Inc.	Albumin fusion proteins
US20050054051A1 (en) *	2001-04-12	2005-03-10	Human Genome Sciences, Inc.	Albumin fusion proteins
US7595172B2 (en)	2001-07-24	2009-09-29	Novo Nordisk A/S	Method for making acylated polypeptides
EP1997829A1 (en) *	2001-12-21	2008-12-03	Human Genome Sciences, Inc.	Albumin fusion proteins
CA2484556A1 (en) *	2001-12-21	2003-07-24	Human Genome Sciences, Inc.	Albumin fusion proteins
ATE492631T1 (de)	2002-09-13	2011-01-15	Cornell Res Foundation Inc	Verwendung von mutationen zur verbesserung von aspergillus-phytasen
CA2513213C (en)	2003-01-22	2013-07-30	Human Genome Sciences, Inc.	Albumin fusion proteins
JP2007532096A (ja)	2003-11-14	2007-11-15	ノボ　ノルディスク　アクティーゼルスカブ	アシル化されたインスリンの製造方法
ATE517119T1 (de)	2003-12-03	2011-08-15	Novo Nordisk As	Einzelketteninsulin
ES2368500T3 (es)	2005-08-16	2011-11-17	Novo Nordisk A/S	Método para producir polipéptidos de insulina maduros.
ES2387955T3 (es)	2006-02-27	2012-10-04	Novo Nordisk A/S	Derivados de insulina
US20080026376A1 (en)	2006-07-11	2008-01-31	Huaming Wang	KEX2 cleavage regions of recombinant fusion proteins
US8198046B2 (en)	2006-07-11	2012-06-12	Danisco Us Inc.	KEX2 cleavage regions of recombinant fusion proteins
JP5864834B2 (ja)	2006-09-22	2016-02-17	ノボ・ノルデイスク・エー／エス	プロテアーゼ耐性のインスリンアナログ
JP5503968B2 (ja)	2006-09-27	2014-05-28	ノボ・ノルデイスク・エー／エス	成熟インスリンポリペプチドの作製方法
WO2008062010A2 (en)	2006-11-22	2008-05-29	Novo Nordisk A/S	Method for making activated carboxypeptidases
WO2009010428A1 (en)	2007-07-16	2009-01-22	Novo Nordisk A/S	Protease stabilized, pegylated insulin analogues
ES2558930T3 (es)	2007-08-13	2016-02-09	Novo Nordisk A/S	Análogos de la insulina de acción rápida
JP5730569B2 (ja)	2007-08-15	2015-06-10	ノボ・ノルデイスク・エー／エス	アシル及びアルキレングリコール部分を有するインスリンアナログ
JP5721432B2 (ja)	2007-08-15	2015-05-20	ノボ・ノルデイスク・エー／エス	アミノ酸含有アルキレングリコール反復単位を含むアシル部を有するインスリン
US8501449B2 (en)	2007-12-04	2013-08-06	Proteon Therapeutics, Inc.	Recombinant elastase proteins and methods of manufacturing and use thereof
CN102037008B (zh)	2008-03-18	2016-08-31	诺沃-诺迪斯克有限公司	蛋白酶稳定化的、酰化胰岛素类似物
WO2009121884A1 (en)	2008-04-01	2009-10-08	Novo Nordisk A/S	Insulin albumin conjugates
AU2010223154B2 (en) *	2009-03-12	2014-10-09	Bigtec Private Limited	A polynucleotide and polypeptide sequence and methods thereof
KR101775000B1 (ko)	2009-06-26	2017-09-04	노보 노르디스크 에이/에스	인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
WO2011064282A1 (en)	2009-11-25	2011-06-03	Novo Nordisk A/S	Method for making polypeptides
CN102791730A (zh)	2010-01-22	2012-11-21	诺沃—诺迪斯克有限公司	低度o-糖基化的fgf21的制备方法
JP6013330B2 (ja)	2010-06-23	2016-10-25	ノヴォノルディスクアー／エス	追加のジスルフィド結合を含有するヒトインスリン
EP2585485A1 (en)	2010-06-23	2013-05-01	Novo Nordisk A/S	Insulin derivatives containing additional disulfide bonds
CN102947331B (zh)	2010-06-23	2016-08-03	诺沃—诺迪斯克有限公司	包含额外的二硫键的胰岛素类似物
WO2012080320A1 (en)	2010-12-14	2012-06-21	Novo Nordisk A/S	Fast-acting insulin in combination with long-acting insulin
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1989
- 1989-03-03 DK DK105489A patent/DK105489D0/da not_active Application Discontinuation
1990
- 1990-02-27 ZA ZA901476A patent/ZA901476B/xx unknown
- 1990-03-01 ES ES90904258T patent/ES2062514T5/es not_active Expired - Lifetime
- 1990-03-01 EP EP90904258A patent/EP0461165B2/en not_active Expired - Lifetime
- 1990-03-01 JP JP2504527A patent/JP2609367B2/ja not_active Expired - Lifetime
- 1990-03-01 AT AT90904258T patent/ATE110414T1/de not_active IP Right Cessation
- 1990-03-01 WO PCT/DK1990/000058 patent/WO1990010075A1/en not_active Ceased
- 1990-03-01 UA UA5010019A patent/UA27706C2/uk unknown
- 1990-03-01 NZ NZ232742A patent/NZ232742A/xx unknown
- 1990-03-01 HU HU902376A patent/HU215538B/hu not_active IP Right Cessation
- 1990-03-01 DK DK90904258.2T patent/DK0461165T3/da active
- 1990-03-01 KR KR1019910701029A patent/KR970005928B1/ko not_active Expired - Fee Related
- 1990-03-01 AU AU52612/90A patent/AU624694B2/en not_active Ceased
- 1990-03-01 CA CA002050336A patent/CA2050336C/en not_active Expired - Fee Related
- 1990-03-01 RU SU5010019/13A patent/RU2194758C2/ru not_active IP Right Cessation
- 1990-03-01 DE DE69011853T patent/DE69011853T3/de not_active Expired - Lifetime
- 1990-03-02 IL IL9360990A patent/IL93609A/en not_active IP Right Cessation
- 1990-03-02 PL PL90284146A patent/PL163532B1/pl not_active IP Right Cessation
- 1990-03-02 DD DD90338353A patent/DD296965A5/de unknown
- 1990-03-02 CZ CS901039A patent/CZ285239B6/cs not_active IP Right Cessation
- 1990-03-02 IE IE74990A patent/IE66469B1/en not_active IP Right Cessation
1991
- 1991-09-02 FI FI914125A patent/FI104985B/fi not_active IP Right Cessation
- 1991-09-02 NO NO913427A patent/NO304236B1/no unknown
1994
- 1994-02-15 US US08/196,887 patent/US5395922A/en not_active Expired - Lifetime
1995
- 1995-03-02 US US08/397,595 patent/US5510249A/en not_active Expired - Lifetime
- 1995-03-02 US US08/397,597 patent/US5514585A/en not_active Expired - Lifetime
- 1995-06-29 HU HU95P/P00592P patent/HU211600A9/hu unknown
1997
- 1997-10-23 NO NO974899A patent/NO974899D0/no unknown

Also Published As

Publication number	Publication date
NO974899L (no)	1997-10-23
HU902376D0 (en)	1991-11-28
EP0461165B1 (en)	1994-08-24
CA2050336C (en)	2000-01-18
DE69011853T3 (de)	1998-06-25
CA2050336A1 (en)	1990-09-04
HUT58370A (en)	1992-02-28
EP0461165B2 (en)	1998-03-25
NZ232742A (en)	1991-10-25
PL163532B1 (pl)	1994-04-29
JP2609367B2 (ja)	1997-05-14
WO1990010075A1 (en)	1990-09-07
NO913427D0 (no)	1991-09-02
DE69011853D1 (de)	1994-09-29
KR920701452A (ko)	1992-08-11
DE69011853T2 (de)	1994-12-15
NO974899D0 (no)	1997-10-23
RU2194758C2 (ru)	2002-12-20
IL93609A (en)	2001-11-25
ES2062514T3 (es)	1994-12-16
NO913427L (no)	1991-11-01
UA27706C2 (uk)	2000-10-16
CZ285239B6 (cs)	1999-06-16
EP0461165A1 (en)	1991-12-18
KR970005928B1 (ko)	1997-04-22
IL93609A0 (en)	1990-12-23
ES2062514T5 (es)	1998-06-01
JPH04504846A (ja)	1992-08-27
IE66469B1 (en)	1995-12-27
FI104985B (fi)	2000-05-15
HU215538B (hu)	1999-01-28
DD296965A5 (de)	1991-12-19
US5395922A (en)	1995-03-07
AU624694B2 (en)	1992-06-18
US5510249A (en)	1996-04-23
US5514585A (en)	1996-05-07
IE900749L (en)	1990-09-03
FI914125A0 (fi)	1991-09-02
NO304236B1 (no)	1998-11-16
AU5261290A (en)	1990-09-26
HU211600A9 (en)	1995-12-28
ZA901476B (en)	1990-11-28
DK105489D0 (da)	1989-03-03
CZ103990A3 (cs)	1999-03-17
ATE110414T1 (de)	1994-09-15

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ATE160587T1 (de)	1997-12-15	Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung
DE69435049D1 (de)	2008-01-24	Chimäre proteine zur vermeidung der komplementaktivierung
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DK114788D0 (da)	1988-03-03	Fremgangsmaade til fremstilling af et fysiologisk aktivt peptid
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Salokangas et al.	1971	Bovine proinsulin: amino acid sequence of the C‐peptide isolated from pancreas
NO912803L (no)	1992-01-20	In vitro prosessering av fusjonsproteiner.
KR920703636A (ko)	1992-12-18	온코스타틴 m 활성을 가진 신규한 단백질과 그 제조과정