[go: up one dir, main page]

DE904095T1 - Chimäre isoprenoidsynthasen und deren verwendungen - Google Patents

Chimäre isoprenoidsynthasen und deren verwendungen

Info

Publication number
DE904095T1
DE904095T1 DE0904095T DE97921142T DE904095T1 DE 904095 T1 DE904095 T1 DE 904095T1 DE 0904095 T DE0904095 T DE 0904095T DE 97921142 T DE97921142 T DE 97921142T DE 904095 T1 DE904095 T1 DE 904095T1
Authority
DE
Germany
Prior art keywords
isoprenoid synthase
chimeric
synthase
chimeric isoprenoid
isoprenoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0904095T
Other languages
English (en)
Inventor
Kyoungwhan Back
Joseph Chappell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Kentucky
Original Assignee
University of Kentucky
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Kentucky filed Critical University of Kentucky
Publication of DE904095T1 publication Critical patent/DE904095T1/de
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (14)

? 'r: &eegr; -^ f* (&Pgr;&Igr; EP 97 92 1142.2 BOARD OF TRUSTEES OF THE UNIVERSITY OF KENTUCKY U.Z.: 171-15/Gr PATENTANSPRÜCHE
1. Ein chimeres Isoprenoidsynthase-Polypeptid, umfassend eine erste Domäne aus einer ersten Isoprenoidsynthase, gebunden an eine zweite Domäne aus einer zweiten heterologen Isoprenoidsynthase, wobei die chimere Isoprenoidsynthase in der Lage ist, die Bildung von Isoprenoid-Reaktionsprodukten zu katalysieren, die in Abwesenheit der zweiten Domäne der zweiten heterologen Isoprenoidsynthase nicht gebildet werden.
2. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die chimere Isoprenoidsynthase in der Lage ist, mindestens zwei verschiedene Isoprenoid-Reaktionsprodukte zu katalysieren.
3. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die zweite Domäne der zweiten heterologen Isoprenoidsynthase auch das Verhältnis der Isoprenoid-Reaktionsprodukte der chimeren Isoprenoidsynthase bestimmt.
4. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die erste Domäne der ersten Isoprenoidsynthase eine Pflanzen-Isoprenoidsynthase und die zweite Domäne der zweiten heterologen Isoprenoidsynthase eine Pflanzen-Isoprenoidsynthase ist.
5. Die chimere Isoprenoidsynthase nach Anspruch 4, wobei die chimere Isoprenoidsynthase ausgewählt ist aus der Gruppe bestehend aus (a) der Tabak- Hyoscyamus-CH4 chimeren Isoprenoidsynthase; (b) der Tabak-Hyoscyamus-CHIO chimeren Isoprenoidsynthase; (c) der Tabak-Hyoscyamus-CHll chimeren Isoprenoidsynthase; (d) der Tabak-Hyoscyamus-CHU chimeren Isoprenoidsynthase; (e) der Tabak-Hyoscyamus-CHtä chimeren Isoprenoidsynthase; oder (f) der Tabak-/^o5cyür/ni/5-CH14 chimeren Isoprenoidsynthase.
6. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die chimere Isoprenoidsynthase die Bildung eines Mittels gegen Pilze katalysiert.
7. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die chimere Isoprenoidsynthase die Bildung eines antibakteriellen Mittels katalysiert.
8. Die chimere Isoprenoidsynthase nach Anspruch 1, wobei die chimere Isoprenoidsynthase die Bildung eines Antitumormittels katalysiert.
9. DNA kodierend ein chimeres Isoprenoidsynthase-Polypeptid nach Anspruch 1.
10. Ein Vektor, enthaltend die DNA nach Anspruch 9.
11. Eine Zelle, enthaltend die DNA nach Anspruch 9.
12. Die Zelle nach Anspruch 11, wobei die Zelle E. coli ist.
13. Ein chimeres Isoprenoidsynthase-Polypeptid, umfassend eine asymmetrisch angeordnete homologe Domäne, wobei die chimere Isoprenoidsynthase in der Lage ist, die Bildung von Isoprenoid-Reaktionsprodukten zu katalysieren, die nicht gebildet werden, wenn diese Domäne an ihrer natürlich vorkommenden Stelle in dem Isoprenoidsynthase-Polypeptid angeordnet ist.
14. Verfahren zur Herstellung eines chimeren Isoprenoidsynthase-Polypeptids, umfassend
(a) die Bereitstellung einer Zelle, die durch DNA nach Anspruch 9 transformiert ist, wobei die DNA zur Expression in der Zelle angeordnet ist;
(b) Züchten der transformierten Zelle unter Bedingungen zur Expression der DNA; und
(c) Isolieren der chimeren Isoprenoidsynthase.
DE0904095T 1996-04-12 1997-04-11 Chimäre isoprenoidsynthasen und deren verwendungen Pending DE904095T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/631,341 US5824774A (en) 1996-04-12 1996-04-12 Chimeric isoprenoid synthases and uses thereof
PCT/US1997/005986 WO1997038703A1 (en) 1996-04-12 1997-04-11 Chimeric isoprenoid synthases and uses thereof

Publications (1)

Publication Number Publication Date
DE904095T1 true DE904095T1 (de) 1999-10-07

Family

ID=24530791

Family Applications (2)

Application Number Title Priority Date Filing Date
DE0904095T Pending DE904095T1 (de) 1996-04-12 1997-04-11 Chimäre isoprenoidsynthasen und deren verwendungen
DE69715192T Expired - Fee Related DE69715192T2 (de) 1996-04-12 1997-04-11 Chimäre isoprenoidsynthasen und deren verwendungen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69715192T Expired - Fee Related DE69715192T2 (de) 1996-04-12 1997-04-11 Chimäre isoprenoidsynthasen und deren verwendungen

Country Status (25)

Country Link
US (2) US5824774A (de)
EP (2) EP1229122A3 (de)
JP (4) JP2000508899A (de)
KR (1) KR20000005385A (de)
AP (1) AP808A (de)
AR (1) AR006638A1 (de)
AT (1) ATE223225T1 (de)
AU (1) AU2726497A (de)
BR (1) BR9708650A (de)
CA (1) CA2250712C (de)
CZ (1) CZ294613B6 (de)
DE (2) DE904095T1 (de)
EA (1) EA001622B1 (de)
ES (1) ES2132046T3 (de)
GR (1) GR990300025T1 (de)
ID (1) ID19769A (de)
IL (2) IL126486A0 (de)
IN (1) IN186316B (de)
OA (1) OA10895A (de)
PL (1) PL329404A1 (de)
PT (1) PT904095E (de)
TR (1) TR199802043T2 (de)
TW (1) TW509724B (de)
WO (1) WO1997038703A1 (de)
ZA (1) ZA973108B (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824774A (en) * 1996-04-12 1998-10-20 Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
US7186891B1 (en) * 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
US8106260B2 (en) * 1996-04-12 2012-01-31 The Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
US5945726A (en) * 1996-12-16 1999-08-31 Micron Technology, Inc. Lateral bipolar transistor
AR022383A1 (es) 1998-09-18 2002-09-04 Univ Kentucky Res Found Sintasas
EP1141275B1 (de) * 1999-01-05 2009-08-12 Trustees Of Boston University Verbessertes klonierungsverfahren
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
AU2001278098A1 (en) 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
CA2954889A1 (en) 2001-03-09 2002-09-19 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
AU2013204829B2 (en) * 2001-03-09 2013-07-04 University Of Kentucky Research Foundation Cytochrome P450s and uses thereof
AU2012202780B2 (en) * 2001-03-09 2014-07-03 University Of Kentucky Research Foundation Cytochrome P450s and Uses Thereof
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) * 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
US7442785B2 (en) 2003-07-24 2008-10-28 The University Of Kentucky Research Foundation Sesquiterpene synthase gene and protein
US8124811B2 (en) * 2007-03-20 2012-02-28 Allylix, Inc. Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one
NZ581473A (en) 2007-06-01 2012-02-24 Scripps Research Inst Use of genetically modified organisms to generate biomass degrading enzymes
CN101889068A (zh) 2007-09-11 2010-11-17 蓝宝石能源公司 用光合生物生产有机产物的方法及其产物和组合物
WO2009036067A2 (en) * 2007-09-11 2009-03-19 Sapphire Energy Molecule production by photosynthetic organisms
WO2009076676A2 (en) * 2007-12-13 2009-06-18 Danisco Us Inc. Compositions and methods for producing isoprene
US8034992B2 (en) * 2008-06-16 2011-10-11 Academia Sinica Gibberellin 2-oxidase genes and uses thereof
US8481286B2 (en) * 2008-08-12 2013-07-09 Allylix, Inc. Method for production of isoprenoid compounds
US9303252B2 (en) 2010-10-29 2016-04-05 Evolva, Inc. Modified valencene synthase polypeptides, encoding nucleic acid molecules and uses thereof
US9550815B2 (en) 2012-01-23 2017-01-24 University Of British Columbia ABC terpenoid transporters and methods of using the same
JP5950253B2 (ja) * 2012-02-13 2016-07-13 住友ゴム工業株式会社 イソプレノイドの製造方法、及びイソプレノイド
US9353385B2 (en) 2012-07-30 2016-05-31 Evolva, Inc. Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof
ES2911701T3 (es) 2012-11-01 2022-05-20 Univ British Columbia Polipéptidos de citocromo P450 y citocromo P450 reductasa, moléculas de ácido nucleico codificantes y usos de los mismos
DK2970934T3 (da) 2013-03-14 2017-11-13 Evolva Inc Valencensynthasepolypeptider, nukleinsyremolekyler, der koder derfor, og anvendelser deraf
CN111621508B (zh) * 2020-06-11 2022-07-01 云南中烟工业有限责任公司 烟草萜类合成酶NtTPS7基因及其载体与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE185042T1 (de) * 1993-04-02 1999-10-15 Pioneer Hi Bred Int Bekämpfung von diabrotica undecimpunctata mittels limonen
CA2118071C (en) * 1993-10-28 2004-09-14 Rodney B. Croteau Dna encoding limonene synthase from mentha spicata
US5824774A (en) * 1996-04-12 1998-10-20 Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof

Also Published As

Publication number Publication date
HK1017275A1 (en) 1999-11-19
KR20000005385A (ko) 2000-01-25
CZ317998A3 (cs) 1999-02-17
DE69715192T2 (de) 2003-04-30
PL329404A1 (en) 1999-03-29
IN186316B (de) 2001-08-04
CA2250712C (en) 2008-07-08
US5824774A (en) 1998-10-20
ID19769A (id) 1998-07-30
OA10895A (en) 2001-10-11
WO1997038703A1 (en) 1997-10-23
US6072045A (en) 2000-06-06
ES2132046T3 (es) 2003-03-16
TR199802043T2 (xx) 1999-01-18
BR9708650A (pt) 1999-08-03
AP9700971A0 (en) 1997-07-31
EA199800917A1 (ru) 1999-04-29
AU2726497A (en) 1997-11-07
JP2012005499A (ja) 2012-01-12
AP808A (en) 2000-02-29
PT904095E (pt) 2002-12-31
ES2132046T1 (es) 1999-08-16
JP2000508899A (ja) 2000-07-18
EP0904095A1 (de) 1999-03-31
EA001622B1 (ru) 2001-06-25
IL126486A0 (en) 1999-08-17
AR006638A1 (es) 1999-09-08
EP0904095A4 (de) 1999-07-07
GR990300025T1 (en) 1999-07-30
EP1229122A3 (de) 2002-10-09
CZ294613B6 (cs) 2005-02-16
IL126486A (en) 2006-04-10
EP0904095B1 (de) 2002-09-04
ATE223225T1 (de) 2002-09-15
JP2015171387A (ja) 2015-10-01
JP2015057074A (ja) 2015-03-26
DE69715192D1 (de) 2002-10-10
TW509724B (en) 2002-11-11
ZA973108B (en) 1997-11-04
EP1229122A2 (de) 2002-08-07
CA2250712A1 (en) 1997-10-23

Similar Documents

Publication Publication Date Title
DE904095T1 (de) Chimäre isoprenoidsynthasen und deren verwendungen
DE3506623C2 (de) Verfahren zum Beschichten eines thermisch widerstandsfähigen Gegenstandes
DE2329486C2 (de) Antibiotika B-41, Verfahren zu deren Herstellung und diese Verbindungen enthaltende acarizide und/oder insektizide Mittel
AT400565B (de) Neues komplexes produkt auf der basis von fasern und füllstoffen, verfahren zu seiner herstellung und seine verwendung
DE2844688B2 (de) Radial-Reaktor zur Durchführung enzymkatalysierter Reaktionen
DE2728575C3 (de) Katalysator für den Austausch von Wasserstoffisotopen zwischen Strömen aus gasförmigem Wasserstoff und flüssigem Wasser zwecks Herstellung von Deuteriumoxid
DE69222901T2 (de) Faser aus Propfcopolymer mit Propylenpolymer als Pfropfgrundlage
DE3044771A1 (de) Magnetisches aufzeichnungsmedium und herstellung desselben
DE2509943A1 (de) Verfahren zur herstellung einer matrix aus kultivierbaren mikroorganismen
DE477532T1 (de) Verfahren zur Herstellung von Restriktionsendonuklease und -methylase Nla III.
DE1290722B (de) Verwendung einer Nickellegierung als Werkstoff fuer Duesen zum Herstellen von Glasfaeden im Schmelzfluss
DE3717076A1 (de) Verfahren zur herstellung von formkoerpern oder ueberzuegen
EP0538714B1 (de) Biologisch abbaubare Faserpräparationsmittel
EP0267205B1 (de) Haltefutter für glasbearbeitungsmaschinen
WO2001081270A2 (de) Keramischer werkstoff
DE3336051A1 (de) Verfahren zur herstellung von 1,2-propandiol, insbesondere d(-)-1,2-propandiol
DE10007654A1 (de) Membran für Brennstoffzellen
DE19738098C1 (de) Hartstoffbeschichtung
DE2038509C3 (de) Warmfeste Nickel-Aluminium-Beryllium-Legierung
DE3434616A1 (de) Oberflaechenschichten aus titan-bor-nitrid-verbindungen und herstellungsverfahren
Hegnauer Comparative phytochemistry and plant taxonomy
EP0050282B1 (de) Aufkohlungs- und korrosionsgeschützte Nickelbasislegierung
JPS55138064A (en) Free-cutting steel having excellent rolling fatigue strength
EP0187928A2 (de) Oberflächenbeschichtete Bauteile von Kernenergieanlagen
EP0078440B1 (de) Austenitischer Stahl mit verbesserter Widerstandsfähigkeit gegen neutroneninduziertes Schwellen