DE806520C - Process for the production of extracts containing peptone and amino acids from press water - Google Patents
Process for the production of extracts containing peptone and amino acids from press waterInfo
- Publication number
- DE806520C DE806520C DED849A DED0000849A DE806520C DE 806520 C DE806520 C DE 806520C DE D849 A DED849 A DE D849A DE D0000849 A DED0000849 A DE D0000849A DE 806520 C DE806520 C DE 806520C
- Authority
- DE
- Germany
- Prior art keywords
- press water
- amino acids
- production
- extracts containing
- containing peptone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims description 19
- 150000001413 amino acids Chemical class 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 7
- 239000001888 Peptone Substances 0.000 title claims description 6
- 108010080698 Peptones Proteins 0.000 title claims description 6
- 235000019319 peptone Nutrition 0.000 title claims description 6
- 239000000284 extract Substances 0.000 title claims description 5
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- 108010019160 Pancreatin Proteins 0.000 claims description 6
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 229940055695 pancreatin Drugs 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 241000252203 Clupea harengus Species 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 235000019514 herring Nutrition 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 239000012190 activator Substances 0.000 claims description 2
- 239000003921 oil Substances 0.000 claims description 2
- 239000010698 whale oil Substances 0.000 claims description 2
- 210000000941 bile Anatomy 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 230000008020 evaporation Effects 0.000 description 4
- 238000001704 evaporation Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 229940066779 peptones Drugs 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000018690 Trypsinogen Human genes 0.000 description 1
- 108010027252 Trypsinogen Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229960004279 formaldehyde Drugs 0.000 description 1
- 235000019256 formaldehyde Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 150000002314 glycerols Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/348—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins obtained from waste materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/002—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from animal waste materials
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
Verfahren zur Herstellung von Pepton und Aminosäure enthaltenden Extrakten aus Preßwasser Bei der Herstellung von Pepton und Aminosäure enthaltenden Extrakten aus Preßwasser, z. B. aus Walöl- oder Heringsölpressen, ist früher vorgeschlagen worden, das Preßwasser unter Druck zu verdampfen, nachdem zur teilweisen oder vollständigen Zersetzung der Proteine Säure zugesetzt ist, und dann das Preßwasser mit Enzymen, z. B. Pepsin, zu behandeln, um möglicherweise zurückbleibende Proteine und Albumosen in Peptone zu überführen. Dieses `'erfahren erfordert beträchtliche Wärmemengen. Außerdem greift die starke Säurelösung das Material in dem Verdampfer während der Verdampfung unter Druck sehr an.Process for the preparation of extracts containing peptone and amino acid from press water in the production of extracts containing peptone and amino acids from press water, z. B. from whale oil or herring oil presses is proposed earlier been to evaporate the press water under pressure after partial or complete Decomposition of the proteins acid is added, and then the press water with enzymes, z. B. pepsin, to treat residual proteins and albumoses to convert into peptones. Experiencing this requires considerable amounts of heat. Also, the strong acid solution attacks the material in the evaporator during the Evaporation under pressure very much.
Die Erfindung betrifft ein Verfahren, durch das die Verdampfung der angesäuerten Lösung unter Druck dadurch vermieden wird, daG) besondere Enzyme als Ersatz für die durch die Verdampfung der angesäuerten Proteinlösung unter Druck bewirkte Hydrolyse benutzt werden.The invention relates to a method by which the evaporation of acidified solution under pressure is avoided by using special enzymes as Substitute for that made by evaporation of the acidified protein solution under pressure effected hydrolysis can be used.
Wenn das Preßwasser in an sich bekannter Weise mit Kalk behandelt wird, sei es, daß eine Pepsinhydrolyse vorhergeht oder nicht, dann wird der p11-Wert gemäß der Erfindung auf 8 bis 9 erhöht und das Preßwasser in bekannter Weise in einem Wasserbade oder durch eine thermostatisch überwachte Wärmequelle auf eine Temperatur von 35 bis .1o° C gebracht. Darauf werden zusätzliche Erizvine, wie Pankreatin und Erepsin, hinzugefügt, bis die günstigste Hydrolysegeschwindigkeit erreicht ist. Der anfänglich zugefügte Kalk wirkt als Aktivator für das Trypsinogen des Pankreatins und crzetigt aktives Trypsin. Dieses Enzym zersetzt einen wesentlichen Teil der in dem Preßwasser enthaltenen ursprünglichen Proteine zu Aminosäuren.When the press water is treated with lime in a manner known per se if pepsin hydrolysis precedes or not, then the p11 value becomes according to the invention increased to 8 to 9 and the press water in a known manner in a water bath or a thermostatically monitored heat source on a Temperature of 35 to .10 ° C brought. Additional erizvins, such as pancreatin, are added to it and Erepsin, added until the most favorable rate of hydrolysis is reached. The initially added lime acts as an activator for the trypsinogen in pancreatin and discontinues active trypsin. This enzyme breaks down an essential one Part of the original proteins contained in the press water into amino acids.
Die in dem Preßwasser enthaltenen Fettstoffe, deren Ausscheidung sonst schwierig ist, werden durch die Lipase des Pankreatins in Fettsäuren und Glycerine zerlegt. Zweckmäßig wird die Lipase durch einen geeigneten Stoff, z. B. Galle, aktiviert, wobei der Aktivierungsstoff hinzugefügt wird, nachdem die Hydrolyse eine Zeitlang, z.13. 1 Stunde, vor sich gegangen ist.The fatty substances contained in the press water, their excretion otherwise Difficult to be converted into fatty acids and glycerols by the lipase of pancreatin disassembled. The lipase is expediently replaced by a suitable substance, e.g. B. Bile, activated, the activating agent being added after the hydrolysis has lasted for a while, z.13. 1 hour has passed.
Die frei gewordenen Fettsäuren bilden mit dem vorhandenen Kalk unlösbare Kalkseifen, die dadurch entfernt werden, daß das hydrolysierte Preß--,vasser in bekannter Weise durch ein Sandfilter getrieben wird. Das Sandfilter hält auch nichthvdrolvsierte Teile der Stoffe zurück.The released fatty acids form insoluble ones with the existing lime Lime soaps, which are removed by the fact that the hydrolyzed press, vasser in is driven through a sand filter in a known manner. The sand filter also holds nonhvdolvsed Parts of the fabrics back.
J Das J filtrierte Wasser wird dann in üblicher Weise in einem Vakuumverdampfer behandelt. Das erhaltene Produkt ist ein Extrakt, in dem die meisten Proteine des Preßwassers vollständig hydroliniert sind.J The J filtered water is then in the usual manner in a vacuum evaporator treated. The product obtained is an extract in which most of the proteins of the Press water are completely hydrolyzed.
Beispiel Es wurden Versuche durch Verdampfen im Vakuum mit i kg Heringspreßwasser angestellt, das o,4% Fettstoffe, ungefähr 3% gelöste Proteine, ungefähr 2% suspendierte Proteinstoffe und einige Mineralsalze (ungefähr o,8%) enthielt.EXAMPLE Experiments were carried out by evaporation in a vacuum with 1 kg of herring press water employed, which suspended 0.4% fatty substances, approximately 3% dissolved proteins, approximately 2% Contained protein substances and some mineral salts (approx. 0.8%).
i. Preßwasser, das nicht mit Kalk und Pankreatin behandelt war, wurde zuerst verdampft, um den Gehalt an Trockensubstanz von etwa 6% auf etwa 50% bei einem spezifischen Gewicht von ungefähr 1,2 bei 20° C zu erhöhen. Eine Stickstoffanalyse zeigte ungefähr 3,70%o Proteinstickstoff und i.o2% Aminosäurestickstoff. ' 2. Preßwasser, das 5 Stunden lang bei einem pii-Wert von 8,5 und einer Temperatur von 35° bis 4o° C mit Kalk und Pankreatin behandelt wurde, wurde dann wie nach Ziffer i eingedampft. Die Analyse zeigte 1,6% Proteinstickstoff und 3,o% Aminosäurestickstoff. Der Aminosäurestickstoff wurde durch Formoltitration nach der Methode von S. P. L. S ö r en s en bestimmt.i. Pressed water that had not been treated with lime and pancreatin was used first evaporated, contributing to the dry matter content from about 6% to about 50% a specific gravity of about 1.2 at 20 ° C. A nitrogen analysis showed approximately 3.70% o protein nitrogen and i.o 2% amino acid nitrogen. '2. Press water, this for 5 hours at a pii value of 8.5 and a temperature of 35 ° to 40 ° C was treated with lime and pancreatin, was then evaporated as in item i. Analysis showed 1.6% protein nitrogen and 3.0% amino acid nitrogen. The amino acid nitrogen was determined by formol titration according to the method of S. P. L. Sö ren s en.
Bei der Behandlung nach dem Verfahren gemäß Ziffer 2 ergeben iooo kg Preßwasser 30 his 35 kg Aminosäuren, etwa io kg Peptone und etwa 7 kg Mineralsalze. Versuche haben jedoch gezeigt, daß eine kleine Zugabe von Erepsin die Hydrolysewirkung erhöht, so daß die Ausbeute bis auf 45 kg Aminosäuren je iooo kg Preßwasser steigt.In the treatment according to the method according to item 2, 100 kg of press water result in 30 to 35 kg of amino acids, about 10 kg of peptones and about 7 kg of mineral salts. Experiments have shown, however, that a small addition of Erepsin increases the hydrolysis effect, so that the yield increases to 45 kg of amino acids per 100 kg of press water.
Claims (2)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NO806520X | 1947-03-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE806520C true DE806520C (en) | 1951-06-14 |
Family
ID=19907106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DED849A Expired DE806520C (en) | 1947-03-27 | 1950-01-26 | Process for the production of extracts containing peptone and amino acids from press water |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE806520C (en) |
-
1950
- 1950-01-26 DE DED849A patent/DE806520C/en not_active Expired
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