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DE69520330D1 - Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen - Google Patents

Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen

Info

Publication number
DE69520330D1
DE69520330D1 DE69520330T DE69520330T DE69520330D1 DE 69520330 D1 DE69520330 D1 DE 69520330D1 DE 69520330 T DE69520330 T DE 69520330T DE 69520330 T DE69520330 T DE 69520330T DE 69520330 D1 DE69520330 D1 DE 69520330D1
Authority
DE
Germany
Prior art keywords
detection
nucleic acid
acid sequences
specific nucleic
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69520330T
Other languages
English (en)
Other versions
DE69520330T2 (de
Inventor
Takahiko Ishiguro
Masami Otsuka
Teruhiko Inoue
Hideo Yawata
Yukio Sugiura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Publication of DE69520330D1 publication Critical patent/DE69520330D1/de
Application granted granted Critical
Publication of DE69520330T2 publication Critical patent/DE69520330T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69520330T 1994-12-01 1995-12-01 Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen Expired - Lifetime DE69520330T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP29866594 1994-12-01
JP18559995A JP3189000B2 (ja) 1994-12-01 1995-07-21 特定核酸配列の検出方法

Publications (2)

Publication Number Publication Date
DE69520330D1 true DE69520330D1 (de) 2001-04-19
DE69520330T2 DE69520330T2 (de) 2001-07-12

Family

ID=26503211

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69520330T Expired - Lifetime DE69520330T2 (de) 1994-12-01 1995-12-01 Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen

Country Status (4)

Country Link
US (1) US5814447A (de)
EP (1) EP0714986B1 (de)
JP (2) JP3189000B2 (de)
DE (1) DE69520330T2 (de)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
WO1997021832A1 (de) * 1995-12-08 1997-06-19 Evotec Biosystems Gmbh Verfahren zur bestimmung von nukleinsäuremolekülen in niedriger konzentration
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
JP3968810B2 (ja) * 1997-01-24 2007-08-29 東ソー株式会社 核酸配列分析方法
JP3337226B2 (ja) * 1997-02-03 2002-10-21 浜松ホトニクス株式会社 Ｒｎａ転写合成モニター方法及びその装置
AU6534898A (en) * 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
US6013442A (en) * 1997-08-05 2000-01-11 Wisconsin Alumni Res Found Direct quantitation of low copy number RNA
US6261781B1 (en) 1997-08-05 2001-07-17 Wisconsin Alumni Research Foundation Direct detection and mutation analysis of low copy number nucleic acids
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6211354B1 (en) 1998-05-06 2001-04-03 Tosch Corporation Optically active DNA probe having phosphonic diester linkage
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
JP4207258B2 (ja) * 1998-08-31 2009-01-14 東ソー株式会社 特定核酸配列の切断方法
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6403313B1 (en) * 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
EP1035214B1 (de) * 1999-03-05 2005-12-21 Tosoh Corporation Eine am 3'-Ende modifizierte Nukleinsäuresonde, geeignet zur Hybridisierung und Detektion über Fluoreszenz
JP2000316587A (ja) * 1999-03-05 2000-11-21 Tosoh Corp 核酸プローブ
JP4552274B2 (ja) * 1999-05-24 2010-09-29 東ソー株式会社 核酸定量分析方法
US6541205B1 (en) * 1999-05-24 2003-04-01 Tosoh Corporation Method for assaying nucleic acid
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US6265170B1 (en) 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
JP2002142765A (ja) * 2000-07-14 2002-05-21 Tosoh Corp 新規ゲノム解析法
EP1223226B1 (de) * 2001-01-11 2009-06-17 Tosoh Corporation Fluoreszierender Farbstoff und Verfahren zur Messung von Nukleinsäuren
US7563618B2 (en) * 2001-03-23 2009-07-21 Geron Corporation Oligonucleotide conjugates
EP1379689B1 (de) * 2001-03-23 2012-09-26 Geron Corporation Oligonukleotidkonjugate
JP2003116543A (ja) * 2001-10-10 2003-04-22 Tosoh Corp 薬効と毒性の評価法
CA2489895A1 (en) * 2002-07-26 2004-02-05 Abbott Laboratories Method of detecting and quantifying hepatitis c virus
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
JP4782668B2 (ja) 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
US20040235032A1 (en) * 2003-05-19 2004-11-25 Canon Kabushiki Kaisha PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method
JP2005080530A (ja) * 2003-09-05 2005-03-31 Tosoh Corp 腸炎ビブリオ菌の耐熱性溶血毒素遺伝子の検出試薬
JP2005080531A (ja) * 2003-09-05 2005-03-31 Tosoh Corp 腸炎ビブリオ菌の耐熱性溶血毒類似溶血毒素遺伝子の検出試薬
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
CA2898814C (en) 2004-08-27 2015-12-29 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
EP1885718B1 (de) 2005-05-11 2017-03-15 Life Technologies Corporation Chemische fluoreszente verbindungen mit hoher selektivität für doppelstrang-dna sowie verfahren zu ihrer verwendung
JP2006328032A (ja) * 2005-05-30 2006-12-07 Fujifilm Holdings Corp 核酸プローブ及び多重鎖核酸蛍光検出方法
CA2622295C (en) 2005-09-12 2019-01-15 The Regents Of The University Of Michigan Recurrent gene fusions in prostate cancer
US9957569B2 (en) * 2005-09-12 2018-05-01 The Regents Of The University Of Michigan Recurrent gene fusions in prostate cancer
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
DE602007012045D1 (de) 2006-06-06 2011-03-03 Gen Probe Inc Markierte oligonukleotide und ihre verwendung in nukleinsäureverstärkungsverfahren
JP2009000063A (ja) 2007-06-22 2009-01-08 Tosoh Corp 改良されたノロウイルスｒｎａ検出方法
ATE533861T1 (de) 2007-07-06 2011-12-15 Univ Michigan Mipol1-etv1-gen-neuanordnungen
EP2167689B1 (de) 2007-07-06 2012-10-31 The Regents Of The University Of Michigan Wiederkehrende genfusionen bei prostatakrebs
EP2259787B1 (de) * 2008-03-25 2015-12-23 The Regents of the University of Michigan Ikki-hemmer-therapien und -screeningverfahren sowie entsprechende ikki-diagnoseverfahren
AU2009246363B2 (en) 2008-05-13 2013-10-24 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
US20110136683A1 (en) 2008-05-28 2011-06-09 Genomedx Biosciences, Inc. Systems and Methods for Expression-Based Discrimination of Distinct Clinical Disease States in Prostate Cancer
WO2010030461A2 (en) * 2008-09-12 2010-03-18 Promega Corporation Assessing expression of endogenous and exogenous genes
JP2010110235A (ja) * 2008-11-04 2010-05-20 Tosoh Corp 核酸プローブを用いた核酸の変異検出方法
AU2010203517B2 (en) 2009-01-09 2012-08-16 The Regents Of The University Of Michigan Recurrent gene fusions in cancer
EP3018218B1 (de) 2009-07-21 2018-01-24 Gen-Probe Incorporated Verfahren für den nachweis von nukleinsäuresequenzen in einem erweiterten dynamischen bereich
CN102712953A (zh) * 2009-09-17 2012-10-03 密歇根大学董事会 前列腺癌中的复发性基因融合物
US9321999B2 (en) 2009-11-19 2016-04-26 Solis Biodyne Oü Compositions for increasing polypeptide stability and activity, and related methods
US8383793B2 (en) 2010-04-15 2013-02-26 St. Jude Children's Research Hospital Methods and compositions for the diagnosis and treatment of cancer resistant to anaplastic lymphoma kinase (ALK) kinase inhibitors
DK2591363T3 (en) 2010-07-07 2017-12-11 Univ Michigan Regents DIAGNOSIS AND TREATMENT OF BREAST CANCER
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
AU2011329753B2 (en) 2010-11-19 2015-07-23 The Regents Of The University Of Michigan ncRNA and uses thereof
US8945556B2 (en) 2010-11-19 2015-02-03 The Regents Of The University Of Michigan RAF gene fusions
US20130267443A1 (en) 2010-11-19 2013-10-10 The Regents Of The University Of Michigan ncRNA AND USES THEREOF
ES2659763T3 (es) 2011-02-14 2018-03-19 The Regents Of The University Of Michigan Composiciones y procedimientos para el tratamiento de obesidad y trastornos relacionados
AU2012312169B2 (en) 2011-09-21 2016-01-14 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
EP2773757B1 (de) 2011-11-04 2019-01-09 Gen-Probe Incorporated Molekulartestreagenzien und verfahren
AU2012330817A1 (en) 2011-11-04 2014-05-22 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
EP2795333A4 (de) 2011-12-20 2016-02-17 Univ Michigan Pseudogene und verwendungen davon
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
AU2013208757A1 (en) 2012-01-09 2014-07-24 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
US9506117B2 (en) 2012-02-21 2016-11-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US20150111758A1 (en) 2012-03-06 2015-04-23 Oslo Universitetssykehus Hf Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US9732387B2 (en) 2012-04-03 2017-08-15 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and Crohn's disease
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
CA2881627C (en) 2012-08-16 2024-09-10 Mayo Foundation For Medical Education And Research CANCER DIAGNOSIS USING BIOMARKERS
WO2014160233A1 (en) 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
EP2971171A4 (de) 2013-03-14 2016-11-02 Abbott Molecular Inc Multiplex-methylierungs-spezifische verstärkersysteme und -verfahren
US9365581B2 (en) 2013-05-02 2016-06-14 The Regents Of The University Of Michigan Deuterated amlexanox
WO2015089438A1 (en) 2013-12-13 2015-06-18 Northwestern University Biomarkers for post-traumatic stress states
EP3151733B1 (de) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Zusammensetzungen und verfahren zur charakterisierung und diagnose von parodontaler erkrankung
JP6507791B2 (ja) * 2015-03-27 2019-05-08 東ソー株式会社 核酸の検出方法
WO2016196478A1 (en) 2015-06-01 2016-12-08 St. Jude Children's Research Hospital Methods and compositions for prognostications and/or clinical management of graft-versus-host disease and transplant rejection
WO2017132538A1 (en) 2016-01-29 2017-08-03 The Regents Of The University Of Michigan Amlexanox analogs
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
US11414708B2 (en) 2016-08-24 2022-08-16 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
WO2018127786A1 (en) 2017-01-06 2018-07-12 Oslo Universitetssykehus Hf Compositions and methods for determining a treatment course of action
AU2018210695B2 (en) 2017-01-20 2024-07-18 The University Of British Columbia Molecular subtyping, prognosis, and treatment of bladder cancer
CA3055925A1 (en) 2017-03-09 2018-09-13 Decipher Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
CA3062716A1 (en) 2017-05-12 2018-11-15 Decipher Biosciences, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor agressiveness
WO2019028285A2 (en) 2017-08-04 2019-02-07 Genomedx, Inc. USE OF SPECIFIC GENE EXPRESSION OF IMMUNE CELLS FOR THE PROGNOSIS OF PROSTATE CANCER AND THE PREDICTION OF SENSITIVITY TO RADIOTHERAPY
US10815484B2 (en) 2017-11-22 2020-10-27 The Regents Of The University Of Michigan Compositions and methods for treating cancer
WO2019202536A1 (en) 2018-04-18 2019-10-24 St. Jude Children's Research Hospital Genotyping assays to identify mutations in xaf1
US12235273B2 (en) 2018-05-04 2025-02-25 Abbott Laboratories HBV diagnostic, prognostic, and therapeutic methods and products
US12139764B2 (en) 2018-11-14 2024-11-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating breast cancer
CN114341627A (zh) * 2019-11-13 2022-04-12 李峰 核酸检测方法及装置
WO2023021330A1 (en) 2021-08-16 2023-02-23 University Of Oslo Compositions and methods for determining a treatment course of action

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4737454A (en) * 1983-07-14 1988-04-12 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
EP0487218B1 (de) * 1990-10-31 1997-12-29 Tosoh Corporation Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
EP0488243A1 (de) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Verfahren zur Extraktion des viralen Genoms von einer von einem lebenden Körper abgeleiteten Probe, die mit dem Virus infiziert ist sowie Nachweis des Genoms
DE69132765T2 (de) * 1990-12-24 2002-02-21 Enzo Diagnostics, Inc. Verfahren zum Nachweis eines Zielpolynukleotids in einer Probe unter Verwendung eines Reagenz, das den Hintergrund verringert, und eine dieses Reagenz enthaltende Zusammensetzung und Kit.
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
FR2686621B1 (fr) * 1992-01-24 1996-03-15 Appligene Sa Procede de detection de mutations par electrophorese en gradient de denaturation d'adn double brin stabilise par photopontage.

Also Published As

Publication number Publication date
EP0714986A1 (de) 1996-06-05
JP3189000B2 (ja) 2001-07-16
JPH08211050A (ja) 1996-08-20
EP0714986B1 (de) 2001-03-14
US5814447A (en) 1998-09-29
JP2001013147A (ja) 2001-01-19
JP3572340B2 (ja) 2004-09-29
DE69520330T2 (de) 2001-07-12

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