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DE60335195D1 - Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon - Google Patents

Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon

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Publication number
DE60335195D1
DE60335195D1 DE60335195T DE60335195T DE60335195D1 DE 60335195 D1 DE60335195 D1 DE 60335195D1 DE 60335195 T DE60335195 T DE 60335195T DE 60335195 T DE60335195 T DE 60335195T DE 60335195 D1 DE60335195 D1 DE 60335195D1
Authority
DE
Germany
Prior art keywords
mutant
phytaseenzyme
phytasease
appa
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335195T
Other languages
English (en)
Inventor
Beat O Blattmann
Aldis Darzins
John M Davis
Lance P Encell
Thomas B Morrison
Gregory T Mrachko
Volker Schellenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Application granted granted Critical
Publication of DE60335195D1 publication Critical patent/DE60335195D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60335195T 2002-08-12 2003-08-11 Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon Expired - Lifetime DE60335195D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40333002P 2002-08-12 2002-08-12
PCT/US2003/025058 WO2004015084A2 (en) 2002-08-12 2003-08-11 Mutant e. coli appa phytase enzymes

Publications (1)

Publication Number Publication Date
DE60335195D1 true DE60335195D1 (de) 2011-01-13

Family

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Family Applications (1)

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DE60335195T Expired - Lifetime DE60335195D1 (de) 2002-08-12 2003-08-11 Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon

Country Status (9)

Country Link
US (1) US7968342B2 (de)
EP (1) EP1546316B1 (de)
CN (2) CN101792749B (de)
AT (1) ATE490309T1 (de)
AU (1) AU2003258157A1 (de)
CA (1) CA2495660A1 (de)
DE (1) DE60335195D1 (de)
DK (1) DK1546316T3 (de)
WO (1) WO2004015084A2 (de)

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CN101792749B (zh) * 2002-08-12 2012-09-05 金克克国际有限公司 大肠杆菌AppA肌醇六磷酸酶突变体
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GB0423139D0 (en) * 2004-10-18 2004-11-17 Danisco Enzymes
US20060194299A1 (en) * 2005-02-04 2006-08-31 Henrik Brinch-Pedersen Method for recycling important nutritional elements from waste
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EP2049669A2 (de) * 2006-08-09 2009-04-22 DSMIP Assets B.V. Sporenoberflächenanzeigen bioaktiver moleküle
EP2617729B1 (de) 2006-09-21 2016-03-16 BASF Enzymes LLC Phytasen, dafür kodierende Nukleinsäuren sowie Verfahren zu deren Herstellung und Verwendung
DE102006053059A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
EP2011858B1 (de) * 2007-07-06 2012-05-30 Chr. Hansen A/S Gallenresistente, große Mengen Phytase absondernde Bakterienzusammensetzung
EP2283124B1 (de) * 2008-04-18 2016-05-25 Danisco US Inc. Buttiauxella sp.-phytase-varianten
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GB0922467D0 (en) * 2009-04-24 2010-02-03 Danisco Feed supplement
HUE031003T2 (en) 2009-05-21 2017-06-28 Basf Enzymes Llc Phytases, nucleic acids and processes encoding them for their production and use
WO2011031736A2 (en) * 2009-09-14 2011-03-17 The Rockefeller University Methods of identifying anti-inflammatory compounds
CN102575237A (zh) * 2009-10-22 2012-07-11 巴斯夫欧洲公司 合成的植酸酶变体
CN104342418B (zh) * 2013-07-24 2017-05-31 东莞泛亚太生物科技有限公司 具有提升的酶活性的植酸酶
TWI478936B (zh) * 2013-07-24 2015-04-01 Genozyme Biotech Inc 具提升酶活性之植酸酶
US9765313B2 (en) * 2013-11-12 2017-09-19 Feed Research Institute, Chinese Academy Of Agricultural Sciences Method for producing a phytase variant with improved thermal stability, and a phytase variant and the use thereof
ES2759083T3 (es) 2014-11-21 2020-05-07 Qingdao Vland Biotech Group Co Ltd Mutantes de fitasa
JP7103948B2 (ja) 2016-03-04 2022-07-20 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
BR112018067862A2 (pt) 2016-03-08 2019-04-16 Basf Enzymes Llc método para melhorar a eficiência de produção de etanol por levedura.
US9605245B1 (en) 2016-06-30 2017-03-28 Fornia BioSoultions, Inc. Phytases and uses thereof
US9528096B1 (en) * 2016-06-30 2016-12-27 Fornia Biosolutions, Inc. Phytases and uses thereof
US10351832B2 (en) 2016-06-30 2019-07-16 Fornia Biosolutions, Inc. Phytases and uses thereof
EP3703661A1 (de) 2017-11-02 2020-09-09 Danisco US Inc. Feste matrixzusammensetzungen mit gesenktem gefrierpunkt für die schmelzgranulation von enzymen
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
BR112020016068A2 (pt) 2018-02-08 2020-12-08 Danisco Us Inc. Partículas cera termicamente resistente matriz para encapsulamento de enzima
US20210238578A1 (en) 2018-04-26 2021-08-05 Danisco Us Inc Inorganic phosphate as a stabilizer for phytase enzymes
CN110484455B (zh) * 2019-06-10 2022-05-24 潍坊康地恩生物科技有限公司 一种稳定高产植酸酶的木霉突变菌株
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Also Published As

Publication number Publication date
CA2495660A1 (en) 2004-02-19
CN100594239C (zh) 2010-03-17
WO2004015084A3 (en) 2004-10-28
DK1546316T3 (da) 2011-02-14
US20060141562A1 (en) 2006-06-29
AU2003258157A1 (en) 2004-02-25
WO2004015084A2 (en) 2004-02-19
EP1546316B1 (de) 2010-12-01
US7968342B2 (en) 2011-06-28
CN101792749B (zh) 2012-09-05
EP1546316A4 (de) 2007-05-02
EP1546316A2 (de) 2005-06-29
AU2003258157A8 (en) 2004-02-25
CN1688690A (zh) 2005-10-26
CN101792749A (zh) 2010-08-04
ATE490309T1 (de) 2010-12-15

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