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DE60335794D1 - Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden - Google Patents

Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden

Info

Publication number
DE60335794D1
DE60335794D1 DE60335794T DE60335794T DE60335794D1 DE 60335794 D1 DE60335794 D1 DE 60335794D1 DE 60335794 T DE60335794 T DE 60335794T DE 60335794 T DE60335794 T DE 60335794T DE 60335794 D1 DE60335794 D1 DE 60335794D1
Authority
DE
Germany
Prior art keywords
methods
expression
vectors
recombinant polypeptides
multigenous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335794T
Other languages
English (en)
Inventor
Arnold H Horwitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xoma Technology Ltd USA
Original Assignee
Xoma Technology Ltd USA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xoma Technology Ltd USA filed Critical Xoma Technology Ltd USA
Application granted granted Critical
Publication of DE60335794D1 publication Critical patent/DE60335794D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE60335794T 2002-03-29 2003-03-31 Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden Expired - Lifetime DE60335794D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36853002P 2002-03-29 2002-03-29
PCT/US2003/010154 WO2004033693A1 (en) 2002-03-29 2003-03-31 Methods vectors plasmids and methods for increasing expression of recombinant polypeptides

Publications (1)

Publication Number Publication Date
DE60335794D1 true DE60335794D1 (de) 2011-03-03

Family

ID=32093683

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60335794T Expired - Lifetime DE60335794D1 (de) 2002-03-29 2003-03-31 Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden

Country Status (9)

Country Link
US (4) US7192737B2 (de)
EP (2) EP2311962B1 (de)
JP (2) JP4554370B2 (de)
AT (1) ATE496134T1 (de)
AU (2) AU2003300588B2 (de)
CA (1) CA2492008C (de)
DE (1) DE60335794D1 (de)
DK (1) DK1492874T3 (de)
WO (1) WO2004033693A1 (de)

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US8278421B2 (en) 2006-03-20 2012-10-02 Xoma Techolology Ltd. Human antibodies specific for gastrin materials and methods
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US8071332B2 (en) 2007-08-10 2011-12-06 Toto Ltd. Recombinant mammal cells, method of producing thereof, and method of producing proteins of interest
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AU2010277786B2 (en) * 2009-07-29 2016-04-14 Sigma-Tau Industrie Farmaceutiche Riunite S.P.A. Improved human long pentraxin 3 expression system and uses thereof
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Also Published As

Publication number Publication date
EP2311962B1 (de) 2013-03-13
EP1492874B1 (de) 2011-01-19
CA2492008A1 (en) 2004-04-22
EP2311962A1 (de) 2011-04-20
AU2009200829A1 (en) 2009-03-19
WO2004033693A1 (en) 2004-04-22
US20030203447A1 (en) 2003-10-30
AU2003300588B2 (en) 2008-12-04
JP2006141415A (ja) 2006-06-08
US7993915B2 (en) 2011-08-09
EP1492874A1 (de) 2005-01-05
CA2492008C (en) 2012-06-26
DK1492874T3 (da) 2011-03-14
JP4554370B2 (ja) 2010-09-29
US20120178126A1 (en) 2012-07-12
JP2005528925A (ja) 2005-09-29
AU2003300588A1 (en) 2004-05-04
US7192737B2 (en) 2007-03-20
US20070281334A1 (en) 2007-12-06
US8497096B2 (en) 2013-07-30
US20080145895A1 (en) 2008-06-19
ATE496134T1 (de) 2011-02-15

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