DE60332641D1 - Methode zur darstellung der replikation von hbv und zum testen der sensibilität von wirkstoffen - Google Patents
Methode zur darstellung der replikation von hbv und zum testen der sensibilität von wirkstoffenInfo
- Publication number
- DE60332641D1 DE60332641D1 DE60332641T DE60332641T DE60332641D1 DE 60332641 D1 DE60332641 D1 DE 60332641D1 DE 60332641 T DE60332641 T DE 60332641T DE 60332641 T DE60332641 T DE 60332641T DE 60332641 D1 DE60332641 D1 DE 60332641D1
- Authority
- DE
- Germany
- Prior art keywords
- hbv
- seq
- primer
- vector
- pharmaceutical product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000010076 replication Effects 0.000 title abstract 4
- 239000013543 active substance Substances 0.000 title 1
- 230000035945 sensitivity Effects 0.000 title 1
- 241000700721 Hepatitis B virus Species 0.000 abstract 16
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 4
- 229940127557 pharmaceutical product Drugs 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 3
- 239000003443 antiviral agent Substances 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 238000003786 synthesis reaction Methods 0.000 abstract 2
- 230000035897 transcription Effects 0.000 abstract 2
- 238000013518 transcription Methods 0.000 abstract 2
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 230000010534 mechanism of action Effects 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 230000002463 transducing effect Effects 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 1
- 230000006648 viral gene expression Effects 0.000 abstract 1
- 230000029812 viral genome replication Effects 0.000 abstract 1
- 239000012873 virucide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20020356188 EP1403385A1 (de) | 2002-09-27 | 2002-09-27 | Methode zur Darstellung der Replikation von HBV und zum Testen der Sensibilität von Wirkstoffen |
| PCT/EP2003/012398 WO2004029301A2 (en) | 2002-09-27 | 2003-09-26 | Method for assaying replication of hbv and testing susceptibility to drugs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60332641D1 true DE60332641D1 (de) | 2010-07-01 |
Family
ID=31970489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60332641T Expired - Lifetime DE60332641D1 (de) | 2002-09-27 | 2003-09-26 | Methode zur darstellung der replikation von hbv und zum testen der sensibilität von wirkstoffen |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20060240405A1 (de) |
| EP (2) | EP1403385A1 (de) |
| CN (1) | CN100562586C (de) |
| AT (1) | ATE468411T1 (de) |
| AU (1) | AU2003294704A1 (de) |
| CA (1) | CA2500188A1 (de) |
| DE (1) | DE60332641D1 (de) |
| ES (1) | ES2346203T3 (de) |
| WO (1) | WO2004029301A2 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1696040A1 (de) * | 2005-02-28 | 2006-08-30 | Eurofins Viralliance, Inc. | Verfahren zur Bestimmung des Genotypes und des Phenotypes von Hepatitis B Viren, die Resistenz gegen antivirale Moleküle zeigen |
| CN101004415B (zh) * | 2007-01-16 | 2011-07-27 | 复旦大学附属华山医院 | 一种测定乙型肝炎病毒阿德福韦耐药变异的方法 |
| CN103114153B (zh) * | 2013-02-05 | 2015-09-02 | 西安交通大学医学院第一附属医院 | 一种鸭乙型肝炎病毒的荧光定量pcr检测方法及试剂 |
| WO2015057919A1 (en) * | 2013-10-18 | 2015-04-23 | The Johns Hopkins University | Transgenic hepatitis b virus (hbv): a new model of hbv infection identifies uqcr10 as a viral replication factor |
| CN107208102B (zh) | 2015-01-27 | 2021-07-06 | 豪夫迈·罗氏有限公司 | 重组hbv cccdna、其产生方法及其用途 |
| CN105907891A (zh) * | 2016-05-27 | 2016-08-31 | 北京旌准医疗科技有限公司 | 检测乙型肝炎患者血液中HBV PgRNA 的方法、试剂盒及其应用 |
| CN106555013A (zh) * | 2016-11-28 | 2017-04-05 | 北京旌准医疗科技有限公司 | Hbv病毒的检测方法、试剂盒及其应用 |
| CN106868045A (zh) * | 2017-02-24 | 2017-06-20 | 重庆市科学技术研究院 | 乙型肝炎e抗原阴性小鼠模型的构建方法 |
| WO2021259345A1 (zh) | 2020-06-24 | 2021-12-30 | 厦门大学 | 靶向HBV cccDNA的药物筛选模型及方法 |
| CN112725535B (zh) * | 2021-02-03 | 2024-05-31 | 江苏安科华捷生物科技有限公司 | 同时检测全长与截短的HBV pgRNA荧光定量PCR试剂盒及其应用 |
| CN114204358B (zh) * | 2021-12-13 | 2022-10-28 | 西安交通大学 | 一种带有电容的家用直流插头插座及工作方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5077192A (en) * | 1988-10-25 | 1991-12-31 | The General Hospital Corporation | Method of detecting antigenic, nucleic acid-containing macromolecular entities |
| EP0787200B1 (de) * | 1994-10-28 | 2005-04-20 | The Trustees Of The University Of Pennsylvania | Rekombinanter adenovirus und methoden zu dessen verwendung |
| US5723319A (en) * | 1995-06-05 | 1998-03-03 | Avid Therapeutics, Inc. | Cultured cell line that inducibly expresses the hepatitis B virus genome, and uses thereof for screening antiviral substances |
| US5856459A (en) * | 1995-06-06 | 1999-01-05 | Hybridon, Inc. | Oligonucleotides specific for hepatitis B virus |
| US5858671A (en) * | 1996-11-01 | 1999-01-12 | The University Of Iowa Research Foundation | Iterative and regenerative DNA sequencing method |
| DE19822287C2 (de) * | 1998-05-18 | 2003-04-24 | Switch Biotech Ag | Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster |
| US6783940B2 (en) * | 2001-10-31 | 2004-08-31 | Applera Corporation | Method of reducing non-specific amplification in PCR |
-
2002
- 2002-09-27 EP EP20020356188 patent/EP1403385A1/de not_active Withdrawn
-
2003
- 2003-09-26 CN CNB038244624A patent/CN100562586C/zh not_active Expired - Fee Related
- 2003-09-26 DE DE60332641T patent/DE60332641D1/de not_active Expired - Lifetime
- 2003-09-26 CA CA002500188A patent/CA2500188A1/en not_active Abandoned
- 2003-09-26 EP EP03785637A patent/EP1543168B1/de not_active Expired - Lifetime
- 2003-09-26 WO PCT/EP2003/012398 patent/WO2004029301A2/en not_active Ceased
- 2003-09-26 AU AU2003294704A patent/AU2003294704A1/en not_active Abandoned
- 2003-09-26 ES ES03785637T patent/ES2346203T3/es not_active Expired - Lifetime
- 2003-09-26 AT AT03785637T patent/ATE468411T1/de not_active IP Right Cessation
- 2003-09-26 US US10/528,833 patent/US20060240405A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2346203T3 (es) | 2010-10-13 |
| EP1543168B1 (de) | 2010-05-19 |
| CA2500188A1 (en) | 2004-04-08 |
| EP1543168A2 (de) | 2005-06-22 |
| US20060240405A1 (en) | 2006-10-26 |
| WO2004029301A2 (en) | 2004-04-08 |
| AU2003294704A1 (en) | 2004-04-19 |
| EP1403385A1 (de) | 2004-03-31 |
| ATE468411T1 (de) | 2010-06-15 |
| CN1701124A (zh) | 2005-11-23 |
| CN100562586C (zh) | 2009-11-25 |
| WO2004029301A3 (en) | 2005-02-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |