[go: up one dir, main page]

DE60126130D1 - In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen - Google Patents

In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen

Info

Publication number
DE60126130D1
DE60126130D1 DE60126130T DE60126130T DE60126130D1 DE 60126130 D1 DE60126130 D1 DE 60126130D1 DE 60126130 T DE60126130 T DE 60126130T DE 60126130 T DE60126130 T DE 60126130T DE 60126130 D1 DE60126130 D1 DE 60126130D1
Authority
DE
Germany
Prior art keywords
immunoglobulin molecules
eukaryotic cells
antigen
identification
manufacture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60126130T
Other languages
English (en)
Other versions
DE60126130T2 (de
Inventor
Maurice Zauderer
S Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of DE60126130D1 publication Critical patent/DE60126130D1/de
Application granted granted Critical
Publication of DE60126130T2 publication Critical patent/DE60126130T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/023—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a poxvirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60126130T 2000-11-17 2001-11-14 In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen Expired - Lifetime DE60126130T2 (de)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US24926800P 2000-11-17 2000-11-17
US249268P 2000-11-17
US26206701P 2001-01-18 2001-01-18
US262067P 2001-01-18
US27142401P 2001-02-27 2001-02-27
US271424P 2001-02-27
US29808701P 2001-06-15 2001-06-15
US298087P 2001-06-15
PCT/US2001/043076 WO2002102855A2 (en) 2000-11-17 2001-11-14 In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells

Publications (2)

Publication Number Publication Date
DE60126130D1 true DE60126130D1 (de) 2007-03-08
DE60126130T2 DE60126130T2 (de) 2007-10-18

Family

ID=27500312

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60126130T Expired - Lifetime DE60126130T2 (de) 2000-11-17 2001-11-14 In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen

Country Status (9)

Country Link
US (2) US7858559B2 (de)
EP (1) EP1340088B1 (de)
JP (1) JP4368196B2 (de)
CN (1) CN1306272C (de)
AT (1) ATE352040T1 (de)
AU (1) AU2001297872B2 (de)
CA (1) CA2429544C (de)
DE (1) DE60126130T2 (de)
WO (1) WO2002102855A2 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) * 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
JP2003528605A (ja) * 2000-03-28 2003-09-30 ユニヴァーシティー オヴ ロチェスター ライブラリーを作成する方法および目的のポリヌクレオチドを選択する方法
AU5311901A (en) * 2000-04-04 2001-10-15 Univ Rochester A gene differentially expressed in breast and bladder cancer and encoded polypeptides
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
EP1830190A3 (de) * 2000-11-17 2008-07-09 University Of Rochester In-vitro-Verfahren zur Herstellung und Identifizierung von Immunoglobulin-Molekülen in eukaryontischen Zellen
ATE352040T1 (de) * 2000-11-17 2007-02-15 Univ Rochester In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen
US20040018573A1 (en) * 2002-04-18 2004-01-29 Power Scott D Production of functional antibodies in filamentous fungi
EP2070949B1 (de) * 2002-06-10 2013-01-16 Vaccinex, Inc. C35-Antikörper und ihre Verwendung zur Behandlung von Krebs
GB0221940D0 (en) * 2002-09-20 2002-10-30 Athena Biodetection Aps Antibody and screening method
ATE431423T1 (de) * 2003-01-21 2009-05-15 Chugai Pharmaceutical Co Ltd Verfahren zum screening der leichten kette eines antikörpers
GEP20094629B (en) 2003-03-19 2009-03-10 Biogen Idec Inc Nogo receptor binding protein
CN1542132A (zh) * 2003-04-30 2004-11-03 上海新霁生物科技有限公司 高效表达治疗肿瘤并含有人恒定区全抗体基因的重组病毒及其用途
CA2548180C (en) * 2003-12-04 2014-02-04 Vaccinex, Inc. Methods of killing tumor cells by targeting internal antigens exposed on apoptotic tumor cells
CA2550933A1 (en) * 2003-12-22 2005-07-14 Amgen Inc. Methods for identifying functional antibodies
US20050266425A1 (en) * 2003-12-31 2005-12-01 Vaccinex, Inc. Methods for producing and identifying multispecific antibodies
PT1776136E (pt) 2004-06-24 2012-12-05 Biogen Idec Inc Tratamento de estados que envolvem desmielinização
US7846438B2 (en) 2004-08-03 2010-12-07 Biogen Idec Ma Inc. Methods of promoting neurite outgrowth with soluble TAJ polypeptides
US20070020238A1 (en) * 2005-06-01 2007-01-25 David Baltimore Method of targeted gene delivery using viral vectors
BRPI0613387A2 (pt) 2005-07-08 2011-01-11 Biogen Idec Inc anticorpo isolado ou fragmento de ligação de antìgeno deste e o seu uso, polinucleotìdeo isolado, composição, vetor, célula hospedeira, anticorpo anti-sp35 e método para a produção do mesmo, polipeptìdeo isolado, método in vitro para redução da inibição do crescimento axonal e método in vitro para inibição do crescimento do colapso do cone
US7790655B2 (en) * 2005-10-14 2010-09-07 Medimmune, Llc Cell display of antibody libraries
AU2006311828B2 (en) 2005-11-04 2013-07-11 Biogen Ma Inc. Methods for promoting neurite outgrowth and survival of dopaminergic neurons
EP2543384A3 (de) 2005-12-02 2013-04-10 Biogen Idec MA Inc. Behandlung von Zuständen mithilfe von Demyelinisation
US8669345B2 (en) 2006-01-27 2014-03-11 Biogen Idec Ma Inc. Nogo receptor antagonists
MX2008012146A (es) * 2006-03-28 2008-10-03 Biogen Idec Inc Anticuerpos anti-receptor 1 de factor de crecimiento tipo insulina (igf-1r) y uso de los mismos.
EP2029172A4 (de) * 2006-06-22 2010-07-28 Vaccinex Inc Antikörper gegen c35 zur behandlung von krebs
AU2007282023B2 (en) * 2006-08-03 2012-09-13 Vaccinex, Inc. Anti-IL-6 monoclonal antibodies and uses thereof
US9382327B2 (en) 2006-10-10 2016-07-05 Vaccinex, Inc. Anti-CD20 antibodies and methods of use
EP1921142A1 (de) 2006-11-07 2008-05-14 Cytos Biotechnology AG Auswahl von menschlichen monoklonalen Antikörpern unter Verwendung von eukaryontischem Zell-Display
DK2099826T3 (da) 2007-01-05 2014-01-20 Univ Zuerich Anti-beta-amyloid-antistof og anvendelser deraf
NO2740744T3 (de) 2007-01-09 2018-08-25
US8128926B2 (en) 2007-01-09 2012-03-06 Biogen Idec Ma Inc. Sp35 antibodies and uses thereof
EP2137308B1 (de) 2007-03-26 2016-08-03 Agenus Inc. Zelloberflächenpräsentation, screening und produktion von interessierenden proteinen
US7807168B2 (en) * 2007-04-10 2010-10-05 Vaccinex, Inc. Selection of human TNFα specific antibodies
AU2008296386A1 (en) * 2007-08-28 2009-03-12 Biogen Idec Ma Inc. Compositions that bind multiple epitopes of IGF-1R
EP2190480A4 (de) * 2007-08-28 2013-01-23 Biogen Idec Inc Anti-igr-1r-antikörper und ihre verwendungen
US7947495B2 (en) * 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
WO2009067660A2 (en) 2007-11-21 2009-05-28 Oregon Health & Science University Anti-factor xi monoclonal antibodies and methods of use thereof
WO2009082485A1 (en) 2007-12-26 2009-07-02 Vaccinex, Inc. Anti-c35 antibody combination therapies and methods
EP2098536A1 (de) * 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
CN102065895A (zh) * 2008-04-11 2011-05-18 比奥根艾迪克Ma公司 抗-igf-1r抗体和其它化合物的治疗联合
CA2729961C (en) 2008-07-09 2018-05-01 Biogen Idec Ma Inc. Li113, li62 variant co2, anti-lingo antibodies
AU2009328505B2 (en) 2008-12-19 2014-11-27 Panima Pharmaceuticals Ag Human anti-alpha-synuclein autoantibodies
CN102341411A (zh) 2008-12-31 2012-02-01 比奥根艾迪克Ma公司 抗-淋巴细胞毒素抗体
EP2411412B1 (de) 2009-03-24 2015-05-27 Teva Biopharmaceuticals USA, Inc. Humanisierte antikörper gegen licht und anwendungen davon
PT2427212T (pt) 2009-05-08 2017-11-20 Vaccinex Inc Anticorpos anti-cd100 e métodos para utilização dos mesmos
WO2011100566A2 (en) 2010-02-12 2011-08-18 Oncomed Pharmaceuticals, Inc. Methods for identifying and isolating cells expressing a polypeptide
US20110250642A1 (en) * 2010-04-12 2011-10-13 Sorrento Therapeutics Method for Displaying Antibodies
EP2572196A4 (de) * 2010-05-18 2014-04-23 Christopher P Marshall Assay zur identifikation von antigenen zur aktivierung von b-zellen-rezeptoren mit neutralisierenden antikörpern
US9963504B2 (en) 2010-09-02 2018-05-08 Vaccinex, Inc. Anti-CXCL13 antibodies and methods of using the same
LT2627672T (lt) 2010-10-11 2018-12-10 Biogen International Neuroscience Gmbh Žmogaus antikūnai prieš tau baltymą
CN103534392A (zh) * 2010-12-01 2014-01-22 默沙东公司 表面锚定的fc-诱饵抗体展示系统
US10106598B2 (en) 2010-12-01 2018-10-23 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
CA2818781C (en) 2010-12-17 2019-10-08 Neurimmune Holding Ag Human anti-sod1 antibodies
AU2011352207B2 (en) * 2010-12-31 2016-03-03 Bioatla, Llc Comprehensive monoclonal antibody generation
ES2664972T3 (es) 2011-06-10 2018-04-24 Medimmune Limited Moléculas de unión anti-Psl de Pseudomonas y usos de las mismas
EA030777B9 (ru) 2011-06-23 2019-02-28 Байоджен Интернэшнл Нейросайенз Гмбх Анти-альфа-синуклеинсвязывающие молекулы
AU2012336028A1 (en) 2011-11-07 2014-06-26 Medimmune, Llc Combination therapies using anti- Pseudomonas Psl and PcrV binding molecules
SG11201403445YA (en) 2011-12-22 2014-07-30 Hoffmann La Roche Full length antibody display system for eukaryotic cells and its use
US9708601B2 (en) * 2012-04-26 2017-07-18 Vaccinex, Inc. Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus
US9796780B2 (en) 2012-05-14 2017-10-24 Biogen Ma Inc. LINGO-2 antagonists for treatment of conditions involving motor neurons
EP3792278A3 (de) 2012-12-21 2021-05-26 Biogen MA Inc. Menschliche anti-tau-antikörper
EP3517545A1 (de) 2012-12-31 2019-07-31 Neurimmune Holding AG Rekombinante menschliche antikörper zur therapie und vorbeugung von erkrankungen im zusammenhang mit polyomavirus
EP2949789B1 (de) 2013-01-24 2021-07-28 AbTLAS Co., Ltd. Auf einer proteinkombination basierende fv-bibliothek und herstellungsverfahren dafür
CN103214580B (zh) * 2013-03-07 2014-12-17 中国人民解放军军事医学科学院基础医学研究所 一种抗Her2免疫细胞因子及其应用
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
AU2015295441B2 (en) 2014-07-29 2020-05-14 Neurimmune Holding Ag Human-derived anti-huntingtin (HTT) antibodies and uses thereof
BR112017006598A2 (en) 2014-09-30 2018-04-17 Neurimmune Holding Ag human derived antidipeptide repeat antibody (dprs)
WO2016112270A1 (en) 2015-01-08 2016-07-14 Biogen Ma Inc. Lingo-1 antagonists and uses for treatment of demyelinating disorders
AU2016239331C1 (en) 2015-03-31 2020-01-30 Medimmune Limited A novel IL33 form, mutated forms of IL33, antibodies, assays and methods of using the same
KR20180042449A (ko) 2015-09-15 2018-04-25 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 T 세포 수용체(tcr) 결합 항체 및 이의 용도
EP3426298A4 (de) 2016-03-10 2019-11-27 Viela Bio, Inc. Ilt7-bindende moleküle und verfahren zur verwendung davon
SG11201808554TA (en) 2016-04-22 2018-11-29 Vaccinex Inc Integral membrane protein display on poxvirus extracellular enveloped virions
JP2018035137A (ja) 2016-07-13 2018-03-08 マブイミューン ダイアグノスティックス エイジーＭａｂｉｍｍｕｎｅ Ｄｉａｇｎｏｓｔｉｃｓ Ａｇ 新規な抗線維芽細胞活性化タンパク質（ｆａｐ）結合薬剤およびその使用
KR102449406B1 (ko) * 2016-08-02 2022-09-29 백시넥스 인코포레이티드 백시니아 바이러스/진핵 세포에서 폴리뉴클레오티드 라이브러리를 생산하기 위한 개선된 방법
CN106905415B (zh) * 2017-02-23 2021-04-20 新疆军区总医院 与耐药宫颈癌癌细胞系Hela细胞膜表面分子结合的多肽
EP3618865A4 (de) 2017-05-05 2021-05-05 Vaccinex, Inc. Menschlicher anti-semaphorin-4d-antikörper
CN107418913B (zh) * 2017-06-14 2020-12-22 湖南省农业生物技术研究中心 一种转化污染土壤中重金属镉的微生物菌剂的应用
EP3887399A1 (de) 2018-11-30 2021-10-06 Institut Gustave-Roussy Kombinationstherapie aus anti-neuropilin-1 und anti-programmierter zelltod-1 zur behandlung von krebs
CN113795513B (zh) 2019-02-13 2025-02-18 布里格姆妇女医院 抗外周淋巴结地址素抗体及其用途
US20200392230A1 (en) * 2019-05-14 2020-12-17 Qlb Biotherapeutics Bispecific anti-cd3 x cd20 antibodies and uses thereof
CA3173521A1 (en) 2020-02-28 2021-09-02 The Brigham And Women's Hospital, Inc. Selective modulation of transforming growth factor beta superfamily signaling via multi-specific antibodies
US11976383B2 (en) 2020-05-06 2024-05-07 Vaccinex, Inc. Integral membrane protein display on poxvirus extracellular enveloped virions
CN117222665A (zh) 2021-04-19 2023-12-12 免疫医疗有限公司 具有改善的稳定性的抗tslp fab
CA3256255A1 (en) 2022-06-10 2023-12-14 Vaccinex, Inc. METHODS FOR SELECTING ANTIBODIES SPECIFIC TO COMPLEX MEMBRANE ANTIGENS
CN119894926A (zh) 2022-08-19 2025-04-25 免疫医疗有限公司 用于检测il-33的测定
AR134366A1 (es) 2023-11-15 2026-01-07 Neurimmune Ag Anticuerpo antitranstiretina, composiciones que comprenden dicho anticuerpo y métodos para tratar o prevenir la amiloidosis mediada por transtiretina

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769330A (en) * 1981-12-24 1988-09-06 Health Research, Incorporated Modified vaccinia virus and methods for making and using the same
US5110587A (en) * 1981-12-24 1992-05-05 Health Research, Incorporated Immunogenic composition comprising synthetically modified vaccinia virus
US4603112A (en) * 1981-12-24 1986-07-29 Health Research, Incorporated Modified vaccinia virus
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0088994B1 (de) * 1982-03-15 1991-06-19 Schering Corporation Hybride DNS, damit hergestellte Bindungszusammensetzung und Verfahren dafür
US7045313B1 (en) * 1982-11-30 2006-05-16 The United States Of America As Represented By The Department Of Health And Human Services Recombinant vaccinia virus containing a chimeric gene having foreign DNA flanked by vaccinia regulatory DNA
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
US5807715A (en) * 1984-08-27 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
EP0247091B1 (de) 1985-11-01 1993-09-29 Xoma Corporation Modulare einheit von antikörpergenen, daraus hergestellte antikörper und verwendung
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5225539A (en) * 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
JPS63152984A (ja) 1986-08-18 1988-06-25 Wakunaga Pharmaceut Co Ltd 抗緑膿菌ヒト型抗体のｌ鎖をコ−ドするｄｎａ
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
WO1988006630A1 (en) 1987-03-02 1988-09-07 Genex Corporation Method for the preparation of binding molecules
CA1341235C (en) 1987-07-24 2001-05-22 Randy R. Robinson Modular assembly of antibody genes, antibodies prepared thereby and use
WO1989001526A1 (en) 1987-08-07 1989-02-23 Genelabs Incorporated Coincidence cloning method and library
US5965405A (en) * 1988-04-16 1999-10-12 Celltech Limited Method for producing Fv fragments in eukaryotic cells
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
ES2052027T5 (es) * 1988-11-11 2005-04-16 Medical Research Council Clonacion de secuencias de dominio variable de inmunoglobulina.
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
EP0478627A4 (en) 1989-05-16 1992-08-19 William D. Huse Co-expression of heteromeric receptors
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US6291160B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291159B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291158B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
AU7247191A (en) * 1990-01-11 1991-08-05 Molecular Affinities Corporation Production of antibodies using gene libraries
US5780225A (en) * 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
EP0585287B1 (de) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Verfahren zur herstellung von spezifischen bindungspaargliedern
US5650150A (en) * 1990-11-09 1997-07-22 Gillies; Stephen D. Recombinant antibody cytokine fusion proteins
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
GB9114468D0 (en) * 1991-07-04 1991-08-21 Wellcome Found Antibody production
EP0597960B1 (de) * 1991-08-10 1999-01-20 Medical Research Council Behandlung von Zellpopulationen
US5445953A (en) * 1991-08-26 1995-08-29 Immuno Aktiengesellschaft Direct molecular cloning of a modified poxvirus genome
ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
ES2341666T3 (es) * 1991-12-02 2010-06-24 Medimmune Limited Produccion de autoanticuerpos de repertorios de segmentos de anticue rpos expresados en la superficie de fagos.
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
DK1621554T4 (da) * 1992-08-21 2012-12-17 Univ Bruxelles Immunoglobuliner blottet for lette kæder
US6005079A (en) * 1992-08-21 1999-12-21 Vrije Universiteit Brussels Immunoglobulins devoid of light chains
ATE245703T1 (de) * 1992-09-22 2003-08-15 Biofocus Discovery Ltd Rekombinante viren, die an ihrer äusseren oberfläche ein nichtvirales polypeptid präsentieren
AU6796094A (en) 1993-04-29 1994-11-21 Raymond Hamers Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of (camelidae)
CN1045820C (zh) * 1993-11-18 1999-10-20 重庆市肿瘤研究所 一种用于测定人ⅲ型前胶原的试剂盒及其测定方法
DE69531148T2 (de) * 1994-01-31 2004-04-29 Trustees Of Boston University, Boston Bibliotheken aus polyklonalen antikörpern
FR2715940B1 (fr) * 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JP3911010B2 (ja) * 1994-04-29 2007-05-09 イムノ・アクテイエンゲゼルシヤフト 必須領域に外来ポリヌクレオチドを有する組換えポックスウイルス
US6010861A (en) * 1994-08-03 2000-01-04 Dgi Biotechnologies, Llc Target specific screens and their use for discovering small organic molecular pharmacophores
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
EP0739981A1 (de) 1995-04-25 1996-10-30 Vrije Universiteit Brussel Variable Fragmente von Immunglobulinen-Verwendung zur therapeutischen oder veterinären Zwecken
AU725609C (en) 1995-08-18 2002-01-03 Morphosys Ag Protein/(poly)peptide libraries
US5916771A (en) * 1996-10-11 1999-06-29 Abgenix, Inc. Production of a multimeric protein by cell fusion method
JP2002514919A (ja) * 1997-04-04 2002-05-21 バイオサイト ダイアグノスティックス，インコーポレイテッド 多価ライブラリーおよびポリクローナルライブラリー
US6057098A (en) * 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
US6872518B2 (en) * 1997-09-22 2005-03-29 University Of Rochester Methods for selecting polynucleotides encoding T cell epitopes
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
EP1036321A1 (de) 1997-12-05 2000-09-20 Intercell Biomedizinische Forschungs- und Entwicklungs GmbH Verfahren und zellen zum "screening" von "combinatorial libraries"
BR9907241A (pt) 1998-01-26 2000-10-17 Unilever Nv Biblioteca de expressão, processo para preparar a mesma, uso de uma fonte não imunizada de sequências de ácido nucleico, e, processos para preparar fragmentos de anticorpos e, para preparar um anticorpo
EP0954978B1 (de) 1998-03-12 2011-11-30 VHsquared Limited Produkten die inaktivierte Hefen oder Schimmel enthalten, die auf ihrer Aussenoberfläche aktive Antikörper haben
AU776865B2 (en) * 1998-11-10 2004-09-23 University Of Rochester T cells specific for target antigens and methods and vaccines based thereon
EP1144616B2 (de) 1999-01-19 2009-01-14 Unilever Plc Verfahren zur herstellung von antikörperfragmenten
CA2372198A1 (en) 1999-05-14 2000-11-23 Medical Research Council Protein scaffold and its use to multimerise monomeric polypeptides
WO2001018058A2 (en) 1999-09-07 2001-03-15 Viventia Biotech Inc. Enhanced phage display library of human vh fragments and methods for producing same
CN1301772A (zh) * 1999-12-30 2001-07-04 中国科学院生物物理研究所 特异性识别发育调控蛋白qBrn－2的抗体及其制备方法和用途
JP2003528605A (ja) * 2000-03-28 2003-09-30 ユニヴァーシティー オヴ ロチェスター ライブラリーを作成する方法および目的のポリヌクレオチドを選択する方法
US20030194696A1 (en) * 2000-03-28 2003-10-16 University Of Rochester Methods of producing a library and methods of selecting polynucleotides of interest
US6410246B1 (en) * 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
WO2002004628A2 (en) * 2000-07-06 2002-01-17 Genvec, Inc. Method of identifying a binding partner of a gene product
ATE352040T1 (de) 2000-11-17 2007-02-15 Univ Rochester In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
US20030104402A1 (en) 2001-01-23 2003-06-05 University Of Rochester Methods of producing or identifying intrabodies in eukaryotic cells
AU2002255495A1 (en) 2001-02-02 2002-08-19 University Of Rochester Methods of identifying regulator molecules
JP4578098B2 (ja) * 2001-10-01 2010-11-10 ダイアックス、コープ 多重鎖真核ディスプレイベクターとその使用
AU2003294234A1 (en) 2002-10-22 2004-05-13 University Of Rochester Methods of producing a library and methods of selecting polynucleotides of interest
US20050266425A1 (en) * 2003-12-31 2005-12-01 Vaccinex, Inc. Methods for producing and identifying multispecific antibodies

Also Published As

Publication number Publication date
EP1340088A2 (de) 2003-09-03
WO2002102855A2 (en) 2002-12-27
CA2429544C (en) 2010-10-19
ATE352040T1 (de) 2007-02-15
CN1306272C (zh) 2007-03-21
US20080167193A1 (en) 2008-07-10
WO2002102855A3 (en) 2003-06-26
EP1340088B1 (de) 2007-01-17
CA2429544A1 (en) 2002-12-27
US8741810B2 (en) 2014-06-03
JP4368196B2 (ja) 2009-11-18
CN1527944A (zh) 2004-09-08
JP2004532645A (ja) 2004-10-28
US20020123057A1 (en) 2002-09-05
US7858559B2 (en) 2010-12-28
AU2001297872B2 (en) 2006-11-09
DE60126130T2 (de) 2007-10-18

Similar Documents

Publication Publication Date Title
DE60126130D1 (de) In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen
WO2005072112A3 (en) Methods for producing and identifying multispecific antibodies
ATE223383T1 (de) Verfahren zur herstellung von 3-haloalkyl-1h- pyrazole
DK1414858T3 (da) Kamelid-enkeltkæde-VHH-antistoffer, fremgangsmåde til fremstilling heraf i et pattedyr samt anvendelser heraf
DE602004025805D1 (de) Verfahren zur herstellung von antikörpern
ATE323110T1 (de) Verfahren zur herstellung von einem cyclodextrin
ATE407138T1 (de) Verfahren zur herstellung interferierender rna- moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle
ATE506374T1 (de) Exprimierungskontrolle unter verwendung von variabelen intergenischen sequenzen
IL183370A0 (en) Monoclonal antibody production by ebv transformation of b cells
DE68921364D1 (de) Verfahren zur Herstellung von Proteinen mittels rekombinanter DNA.
DE59708838D1 (de) Spiegelselektion und spiegelevolution von nucleinsäuren
ATE404588T1 (de) Verfahren zur herstellung von chondroitinsulfaten aus k4-polysaccharid und so hergestellte produkte
ATE468390T1 (de) System und verfahren zur produktion von antikörpern in der pflanzenzellkultur
ATE448863T1 (de) Verfahren zur gewinnung von gallium-68 und dessen verwendung und hilfsmittel zur durchführung dieses verfahrens
DE60335191D1 (de) Verfahren zur förderung der homologen rekombination somatischer zellen und verfahren zur konstruktion eines spezifischen antikörpers
DE502006005581D1 (de) Ukmischungen, ein verfahren zu deren herstellung, verfahren zur herstellung von verbund-formteilen und deren verwendung
DE69534595D1 (de) Methode zur proliferation und differenzierung von b-zellen und verwendungen dafür
DE69718332D1 (de) ZnS-PHOTOKATALYSATOR, SEINE HERSTELLUNG UND VERFAHREN ZUR PRODUKTION VON WASSERSTOFF DURCH VERWENDUNG DESSELBEN
ATE275635T1 (de) Verfahren zur herstellung von beta-karotin
ATE363490T1 (de) Methode zur produktion monoklonaler antikörper
EP1830190A3 (de) In-vitro-Verfahren zur Herstellung und Identifizierung von Immunoglobulin-Molekülen in eukaryontischen Zellen
ATE305170T1 (de) Herstellungsverfahren für eine gasentladungslampe
DE50110561D1 (de) Non-metallocene, verfahren zur herstellung von diesen und deren verwendung zur polymerisation von olefinen
ATE464375T1 (de) Verfahren zur herstellung von virusmaterial
ATE272669T1 (de) Neue thioether-derivate, verfahren zu ihrer herstellung und ihre verwendung

Legal Events

Date Code Title Description
8364 No opposition during term of opposition