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DE60112276D1 - Elektrophoretische trennung von verbindungen - Google Patents

Elektrophoretische trennung von verbindungen

Info

Publication number
DE60112276D1
DE60112276D1 DE60112276T DE60112276T DE60112276D1 DE 60112276 D1 DE60112276 D1 DE 60112276D1 DE 60112276 T DE60112276 T DE 60112276T DE 60112276 T DE60112276 T DE 60112276T DE 60112276 D1 DE60112276 D1 DE 60112276D1
Authority
DE
Germany
Prior art keywords
compounds
electrophoretic separation
electrophoretic
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60112276T
Other languages
English (en)
Other versions
DE60112276T2 (de
Inventor
D Faupel
H Girault
Frederic Reymond
Alexandra Ros
Stephane Rossier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ECOLE POLYTECH, Ecole Polytechnique Federale de Lausanne EPFL filed Critical ECOLE POLYTECH
Application granted granted Critical
Publication of DE60112276D1 publication Critical patent/DE60112276D1/de
Publication of DE60112276T2 publication Critical patent/DE60112276T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/0015—Field flow fractionation characterised by driving force
    • G01N2030/0035—Field flow fractionation characterised by driving force electrical field
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N2030/285—Control of physical parameters of the fluid carrier electrically driven carrier

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
DE60112276T 2000-05-05 2001-05-04 Elektrophoretische trennung von verbindungen Expired - Lifetime DE60112276T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0010957 2000-05-05
GBGB0010957.9A GB0010957D0 (en) 2000-05-05 2000-05-05 Compound & method
PCT/EP2001/005704 WO2001086279A1 (en) 2000-05-05 2001-05-04 Electrophoretic separation of compounds

Publications (2)

Publication Number Publication Date
DE60112276D1 true DE60112276D1 (de) 2005-09-01
DE60112276T2 DE60112276T2 (de) 2006-04-20

Family

ID=9891094

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60112276T Expired - Lifetime DE60112276T2 (de) 2000-05-05 2001-05-04 Elektrophoretische trennung von verbindungen

Country Status (7)

Country Link
US (1) US7615354B2 (de)
EP (1) EP1281073B1 (de)
JP (1) JP4754759B2 (de)
AU (2) AU2001267455B2 (de)
DE (1) DE60112276T2 (de)
GB (1) GB0010957D0 (de)
WO (1) WO2001086279A1 (de)

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GB0121189D0 (en) 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
GB0226160D0 (en) * 2002-11-08 2002-12-18 Diagnoswiss Sa Apparatus for dispensing a sample in electrospray mass spectrometers
FR2847343B1 (fr) * 2002-11-18 2005-12-09 Centre Nat Rech Scient Dispositif et procede d'electrophorese
GB0300820D0 (en) * 2003-01-14 2003-02-12 Diagnoswiss Sa Membrane-microchannel strip
AU2004272746B2 (en) * 2003-09-15 2009-12-03 Diagnoswiss S.A. Microfluidic flow monitoring device
WO2005029061A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Extraction of molecules using frame
FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
JP4492212B2 (ja) * 2004-05-20 2010-06-30 株式会社島津製作所 等電点電気泳動チップ及び装置
JP4424076B2 (ja) * 2004-06-04 2010-03-03 株式会社島津製作所 脱塩用マイクロチップおよび脱塩装置
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
US20070240986A1 (en) * 2004-11-12 2007-10-18 Diagnoswiss S.A. Microfluidic Device with Minimized Ohmic Resistance
ITPD20040301A1 (it) * 2004-11-26 2005-02-26 Dimensional Srl P Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale
WO2006063625A1 (en) * 2004-12-17 2006-06-22 Agilent Technologies, Inc. Fractionation using electro elution
DE602005027623D1 (de) 2005-11-02 2011-06-01 Agilent Technologies Inc Kraftunterstützte probenwiedergewinnung in der gelelektrophorese
GB0607205D0 (en) * 2006-04-10 2006-05-17 Diagnoswiss Sa Miniaturised biosensor with optimized anperimetric detection
EP2013613A4 (de) 2006-04-27 2010-09-01 Ge Healthcare Bio Sciences Ab Elektrodenbrücke
ES2651352T3 (es) * 2006-06-20 2018-01-25 Becton Dickinson And Company Método y dispositivo para separación y eliminación de ciertas proteínas y partículas utilizando electroforesis
GB0712795D0 (en) * 2007-07-02 2007-08-08 Ecole Polytechnique Federale De Solid phase extraction and ionization device
EP2146200A1 (de) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
EP2773221B1 (de) 2011-11-04 2019-01-09 Bio-Rad Laboratories, Inc. Gleichzeitige reinigung von zellkomponenten
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013078236A1 (en) 2011-11-22 2013-05-30 Haralampu Stephen G Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
EP2801103B1 (de) 2012-01-06 2018-10-03 Ecole Polytechnique Federale de Lausanne (EPFL) Elektrostatisches-spray-ionisationsverfahren
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CN104411383A (zh) * 2012-05-03 2015-03-11 米迪缪尼有限公司 分析样品组分的方法
JP6507424B2 (ja) * 2015-02-26 2019-05-08 三菱日立パワーシステムズ株式会社 塩分測定方法及び塩分捕集装置
WO2017044614A1 (en) * 2015-09-11 2017-03-16 The Regents Of The University Of California Isoelectric focusing arrays and methods of use thereof
US9702851B1 (en) * 2016-06-17 2017-07-11 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use
CN120571413A (zh) * 2019-10-09 2025-09-02 普诺森公司 用于具有顺序pH调整的单通道自由流电泳的方法
US20220334033A1 (en) * 2021-04-20 2022-10-20 Brown University Electrical dissociation of tissue samples into single cells and/or smaller groups of cells

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US2815320A (en) * 1953-10-23 1957-12-03 Kollsman Paul Method of and apparatus for treating ionic fluids by dialysis
DE2051715B2 (de) * 1970-10-21 1976-01-15 Trennkammer fuer die kontinuierliche ablenkungselektrophorese
US3719580A (en) 1971-06-04 1973-03-06 R Roberts Electrophoretic apparatus
GB1422118A (en) 1972-01-04 1976-01-21 Bovril Ltd Vertical gel electrophoresis apparatus
US3888758A (en) 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4148703A (en) * 1976-02-11 1979-04-10 Morton Weintraub Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system
CA1238296A (en) 1984-08-02 1988-06-21 Claude Hamelin Electrophoresis system for multiple agarose slab gels
ES2037273T3 (es) * 1987-04-11 1993-06-16 Ciba-Geigy Ag Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso.
US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5104512A (en) 1990-05-14 1992-04-14 Labintelligence, Inc. Gel electrophoresis system
US5149418A (en) 1991-09-04 1992-09-22 Apogee Designs, Ltd. Vertical gel electrophoresis apparatus having universal gel assembly support structure
NO315930B1 (no) * 1995-01-18 2003-11-17 Picower Inst For Medical Res T Anvendelse av tiazoliumforbindelser ved fremstilling av farmasöytiske preparater, preparater som inneholder forbindelsene, samt nyetiazoliumforbindelser
IT1272932B (it) 1995-01-24 1997-07-01 Pier Giorgio Righetti Reattore ad enzima immobilizzato
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
CA2192262C (en) 1995-12-08 2011-03-15 Yoshihide Hayashizaki Method for purification and transfer to separation/detection systems of dna sequencing samples and plates used therefor
US5773645A (en) * 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
AUPP576598A0 (en) 1998-09-07 1998-10-01 Life Therapeutics Limited Cassette for macromolecule purification
JP2003501639A (ja) 1999-06-03 2003-01-14 ユニバーシティ オブ ワシントン 横断電気泳動および等電点電気泳動法のための微小流体デバイス
US6638408B1 (en) 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis

Also Published As

Publication number Publication date
EP1281073A1 (de) 2003-02-05
US7615354B2 (en) 2009-11-10
JP2003532894A (ja) 2003-11-05
JP4754759B2 (ja) 2011-08-24
US20030104449A1 (en) 2003-06-05
AU2001267455B2 (en) 2005-12-08
DE60112276T2 (de) 2006-04-20
EP1281073B1 (de) 2005-07-27
WO2001086279A1 (en) 2001-11-15
GB0010957D0 (en) 2000-06-28
AU6745501A (en) 2001-11-20

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