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DE17196350T1 - Verhindern der disulfid-bindungsreduktion während der rekombinanten herstellung von polypeptiden - Google Patents

Verhindern der disulfid-bindungsreduktion während der rekombinanten herstellung von polypeptiden Download PDF

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DE17196350T1
DE17196350T1 DE17196350.7T DE17196350T DE17196350T1 DE 17196350 T1 DE17196350 T1 DE 17196350T1 DE 17196350 T DE17196350 T DE 17196350T DE 17196350 T1 DE17196350 T1 DE 17196350T1
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Yung-Hsiang Kao
Melody Trexher Schmidt
Michael W. Laird
Rita L. Wong
Daniel P. Hewitt
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Abstract

Verfahren zur Prävention der Reduktion einer Disulfidbindung in einem in einer rekombinanten Wirtszelle exprimierten Polypeptid, umfassend das Ergänzen der Zellkulturflüssigkeit der rekombinanten Wirtszelle vor oder nach dem Gewinnen derselben mit einem Thioredoxin-Inhibitor.

Claims (25)

  1. Verfahren zur Prävention der Reduktion einer Disulfidbindung in einem in einer rekombinanten Wirtszelle exprimierten Polypeptid, umfassend das Ergänzen der Zellkulturflüssigkeit der rekombinanten Wirtszelle vor oder nach dem Gewinnen derselben mit einem Thioredoxin-Inhibitor.
  2. Verfahren nach Anspruch 1, wobei der Thioredoxin-Inhibitor Folgendes ist: (i) ein direkter Inhibitor von Thioredoxin; (ii) ein spezifischer Inhibitor von Thioredoxinreduktase, (iii) ein Metallion; (iv) ein Inhibitor von G6PD; (v) ein Inhibitor von Hexokinaseaktivität; (vi) Cystin, Cystein oder oxidiertes Glutathion; (vii) ein Antikörper, der spezifisch an eine Thioredoxinreduktase bindet; oder (viii) eine Maßnahme, die indirekt zur Hemmung von Thioredoxinaktivität führt.
  3. Verfahren nach Anspruch 2(i), wobei der Thioredoxin-Inhibitor ein Alkyl-2-imidazolyldisulfid oder ein Naphthochinonspiroketal-Derivat ist.
  4. Verfahren nach Anspruch 2(ii), wobei der Thioredoxin-Inhibitor ein Goldkomplex ist.
  5. Verfahren nach Anspruch 4, wobei der Goldkomplex Aurothioglucose (ATG) oder Aurothiomalat (ATM) ist und das ATG oder das ATM gegebenenfalls in einer Konzentration (a) zwischen etwa 0,1 mM und etwa 1 mM oder (b) von zumindest dem 4-Fachen der Thioredoxinreduktase-Konzentration in der Kulturflüssigkeit vor oder nach dem Gewinnen derselben zugesetzt wird.
  6. Verfahren nach Anspruch 2(iii), wobei das Metallion aus der aus Hg2+, Cu2+, Zn2+, Co2+ und Mn2+ bestehenden Gruppe ausgewählt ist.
  7. Verfahren nach Anspruch 6, wobei das Metallion Cu2+ ist, das in Form von Kupfer(II)-sulfat vorliegt.
  8. Verfahren nach Anspruch 7, wobei das Kupfer(II)-sulfat (a) als Pentahydrat oder in wasserfreier Form vorliegt; (b) in einer Konzentration zwischen etwa 5 µM und etwa 100 µM zugesetzt wird; (c) in einer Konzentration zwischen etwa 10 µM und etwa 80 µM zugesetzt wird; (d) in einer Konzentration zwischen etwa 15 µM und etwa 50 µM zugesetzt wird; oder (e) in einer Konzentration von zumindest dem 2-Fachen der Thioredoxin-Konzentration in der Kulturflüssigkeit vor oder nach dem Gewinnen derselben zugesetzt wird
  9. Verfahren nach Anspruch 2(iv), wobei der Thioredoxin-Inhibitor aus der aus Pyridoxal-5'-phosphat, 1-Fluor-2,4-dinitrobenzol, Dehydroepiandrosteron (DHEA) und Epiandrosteron (EA) bestehenden Gruppe ausgewählt ist.
  10. Verfahren nach Anspruch 9, wobei das DHEA in einer Konzentration (a) zwischen etwa 0,05 mM und etwa 5 mM oder (b) zwischen etwa 0,1 mM und etwa 2,5 mM zugesetzt wird.
  11. Verfahren nach Anspruch 2(v), wobei der Thioredoxin-Inhibitor (a) ein Metallionen-Chelatbildner ist; (b) aus der aus Sorbose-1-phosphat, Polyphosphaten, 6-Desoxy-6-fluor-glucose, 2-C-Hydroxymethylglucose, Xylose oder Lyxose bestehenden Gruppe ausgewählt ist.
  12. Verfahren nach Anspruch 11(a), wobei der Metallionen-Chelatbildner Ethylendiamintetraessigsäure (EDTA) ist.
  13. Verfahren nach Anspruch 12, wobei die EDTA in einer Konzentration (i) zwischen etwa 5 mM und etwa 60 mM, (ii) zwischen etwa 10 mM und etwa 50 mM; oder (iii) zwischen etwa 20 mM und etwa 40 mM zugesetzt wird.
  14. Verfahren nach Anspruch 2(vi), wobei das Cystin, Cystein oder oxidierte Glutathion in einer Konzentration von zumindest etwa dem 40-Fachen der Konzentration des Polypeptids in der Kulturflüssigkeit vor oder nach dem Gewinnen derselben zugesetzt wird.
  15. Verfahren nach Anspruch 2(viii), wobei die Maßnahme das Belüften der gewonnenen Kulturflüssigkeit der rekombinanten Wirtszelle oder das Senken des pHs der gewonnenen Kulturflüssigkeit der rekombinanten Wirtszellen ist.
  16. Verfahren nach einem der Ansprüche 1 bis 14, das weiters den Schritt (i) des Belüftens der gewonnenen Kulturflüssigkeit der rekombinanten Wirtszelle oder (ii) des Senkens des pHs der gewonnenen Kulturflüssigkeit der rekombinanten Wirtszellen umfasst.
  17. Verfahren zur Verhinderung der Reduktion einer Disulfidbindung eines Antikörpers oder eines biologisch funktionellen Fragments davon, der/das in einer rekombinanten Wirtszelle exprimiert wird, umfassend das Verringern des Expressionslevels eines Enyzms des Trx-Systems in der eukaryotischen Wirtszelle durch: (a) ein Nucleinsäuremolekül, das ein Antisense-Nucleotid umfasst, oder (b) eine interferierende RNA; und das Gewinnen des Antikörpers oder dessen biologisch funktionellen Fragments, wodurch die Reduktion der Disulfidbindung des Antikörpers oder dessen biologisch funktionellen Fragments im geernteten Zellkulturfluid (HCCF) verhindert wird.
  18. Verfahren nach Anspruch 17, wobei das Enzym Thioredoxinreduktase, G6PD oder Hexokinase ist.
  19. Verfahren nach einem der Ansprüche 1 bis 16, wobei das Polypeptid ein Antikörper oder ein biologisch funktionelles Fragment eines Antikörpers ist.
  20. Verfahren nach einem der Ansprüche 17 bis 19, wobei (i) das biologisch funktionelle Fragment des Antikörpers aus der aus Fab, Fab‘, F(ab‘)2, scFv, (scFv)2, dAb, Fragmenten komplementaritätsbestimmender Regionen (CDR), unverzweigten Antikörpern, Einzelketten-Antikörpermolekülen, Minibodys, Diabodys und multispezifischen Antikörpern, gebildet aus Antikörperfragmenten, bestehenden Gruppe ausgewählt ist oder (ii) der Antikörper oder das Antikörperfragment ein therapeutischer Antikörper oder ein biologisch funktionelles Fragment davon ist.
  21. Verfahren nach Anspruch 20(ii), wobei der therapeutische Antikörper aus der aus Anti-HER2-Antikörpern; Anti-CD20-Antikörpern; Anti-IL-8-Antikörpern; Anti-VEGF-Antikörpern; Anti-CD40-Antikörpern; Anti-CD11a-Antikörpern; Anti-CD18-Antikörpern; Anti-IgE-Antikörpern; Anti-Apo-2-Rezeptor-Antikörpern; Anti-Gewebefaktor-(TF-) Antikörpern; Anti-Mensch-α4β7-Integrin-Antikörpern; Anti-EGFR-Antikörpern; Anti-CD3-Antikörpern; Anti-CD25-Antikörpern; Anti-CD4-Antikörpern; Anti-CD52-Antikörpern; Anti-Fc-Rezeptor-Antikörpern; Anti-karzinoembryonisches-Antigen- (CEA-) Antikörpern; Antikörpern, die gegen Brustepithelzellen gerichtet sind; Antikörpern, die an Dickdarmkarzinomzellen binden; Anti-CD38-Antikörpern; Anti-CD33-Antikörpern; Anti-CD22-Antikörpern; Anti-EpCAM-Antikörpern; Anti-GpIIb/IIIa-Antikörpern; Anti-RSV-Antikörpern; Anti-CMV-Antikörpern; Anti-HIV-Antikörpern; Anti-Hepatitis-Antikörpern; Anti-CA125-Antikörpern; Anti-αvβ3-Antikörpern; Anti-Human-Nierenzellenkarzinom-Antikörpern; Anti-Humen-17-1A-Antikörpern; Anti-Humen-Kolorektaltumor-Antikörpern; Anti-Human-Melanom-Antikörper R24, der gegen GD3-Gangliosid gerichtet ist; Anti-Human-Plattenepithelzellkarzinom-; und Anti-Human-Leukozytenantigen- (HLA-) Antikörpern und Anti-HLA-DR-Antikörpern bestehenden Gruppe ausgewählt ist.
  22. Verfahren nach Anspruch 20(ii), wobei der therapeutische Antikörper ein Antikörper ist, der an Folgendes bindet: (a) einen HER-Rezeptor, wobei der HER-Rezeptor gegebenenfalls HER2 ist und der therapeutische Antikörper eine Sequenz einer variablen Schwer- und/oder Leichtkettendomäne umfasst, die aus der aus Seq.-ID Nr. 16, 17, 18 und 19 bestehenden Gruppe ausgewählt ist; (b) VEGF, wobei der therapeutische Antikörper gegebenenfalls eine Sequenz einer variablen Schwer- und/oder Leichtkettendomäne umfasst, die aus der aus Seq.-ID Nr. 20 bis 55 bestehenden Gruppe ausgewählt ist; (c) IgE; (d) CD20, wobei der therapeutische Antikörper gegebenenfalls eine Sequenz einer variablen Schwer- und/oder Leichtkettendomäne umfasst, die aus der aus Seq.-ID Nr. 1 bis 15 bestehenden Gruppe ausgewählt ist; (e) CD11a, wobei der therapeutische Antikörper gegebenenfalls eine Sequenz einer variablen Schwer- und/oder Leichtkettendomäne umfasst, die aus der aus Seq.-ID Nr. 26 bis 29 bestehenden Gruppe ausgewählt ist; (f) CD40; oder (g) DR5, wobei der therapeutische Antikörper gegebenenfalls aus der aus den Apomabs 1.1, 2.1, 3.1, 4.1, 5.1, 5.2, 5.3, 6.1, 6.2, 6.3, 7.1, 7.2, 7.3, 8.1, 8.3, 9.1, 1.2, 2.2, 3.2, 4.2, 5.2, 6.2, 7.2, 8.2, 9.2, 1.3, 2.2, 3.3, 4.3, 5.3, 6.3, 7.3, 8.3, 9.3 und 25.3 bestehenden Gruppe ausgewählt ist.
  23. Verfahren nach einem der Ansprüche 1 bis 16, wobei das Polypeptid ein therapeutisches Polypeptid ist und wobei das therapeutische Polypeptid gegebenenfalls aus der aus einem Wachstumshormon, einschließlich des menschlichen Wachstumshormons und des Rinderwachstumshormons; dem Wachstumshormon-freisetzenden Faktor; Parathormon; Schilddrüsenstimulationshormon; Lipoproteinen; α-1-Antitrypsin; Insulin-A-Kette; Insulin-B-Kette; Proinsulin; follikelstimulierendem Hormon; Calcitonin; luteinisierendem Hormon; Glucagon; Gerinnungsfaktoren wie Faktor VIIIC, Faktor IX, Gewebefaktor und von-Willebrand-Faktor; Anti-Gerinnungsfaktoren wie Protein C; atrialem natriuretischem Faktor; Lungentensid; einem Plasminogenaktivator wie Urokinase oder menschlichem Urin- oder Gewebetyp-Plasminogen-Aktivator (t-PA); Bombesin; Thrombin; hämatopoietischem Wachstumsfaktor; Tumornekrosefaktor-α und -β; Enkephalinase; RANTES (reguliert bei Aktivierung, normalerweise T-Zellen-exprimiert und -sekretiert); menschlichem Makrophagenentzündungsprotein (MIP-1-α); einem Serumalbumin wie menschlichem Serumalbumin; Müllerscher Inhibitionssubstanz; Relaxin-A-Kette; Relaxin-B-Kette; Prorelaxin; Mäuse-Gonadotropin-assoziiertem Peptid; einem mikrobiellen Protein wie β-Lactamase; DNase; IgE; einem zytotoxischen T-Lymphozyten-assoziierten Antigen (CTLA) wie CTLA-4; Inhibin; Activin; Gefäßendothelwachstumsfaktor (VEGF); Rezeptoren für Hormone oder Wachstumsfaktoren; Protein A oder D; rheumatoiden Faktoren; einem neurotrophen Faktor wie knochenabgeleiteter neurotropher Faktor (BDNF), Neurotrophin-3, -4, -5 oder -6 (NT-3, NT-4, NT-5 oder NT-6) oder einem Nervenwachstumsfaktor wie NGF-β; plättchenabgeleitetem Wachstumsfaktor (PDGF); Fibroblastenwachstumsfaktor wie aFGF und bFGF; Epidermiswachstumsfaktor (EGF); transformierendem Wachstumsfaktor (TGF) wie TGF-α und TGF-β, einschließlich TGF-β1, TGF-β2, TGF-β3, TGF-β4 oder TGF-β5; insulinähnlichem Wachstumsfaktor-I und -II (IGF-I und IGF-II); des(1-3)-IGF-I (Hirn-IGF-I); Bindungsproteinen für insulinähnlichen Wachstumsfaktor; CD-Proteinen wie CD3, CD4, CD8, CD19, CD20, CD34 und CD40; Erythropoietin; osteoinduktiven Faktoren; Immuntoxinen; einem knochenmorphogenetischen Protein (BMP); einem Interferon wie Interferon-α, -β und -γ; koloniestimulierenden Faktoren (CSFs), z.B. M-CSF, GM-CSF und G-CSF; Interleukinen (ILs), z.B. IL-1 bis IL-10; Superoxiddismutase; T-Zellen-Rezeptoren; Oberflächenmembranproteinen; Abbaubeschleunigungsfaktor; viralen Antigenen wie z.B. eines Teils der AIDS-Hülle; Transportproteinen; Homing-Rezeptoren; Addressinen; Regulationsproteinen; Integrinen wie CD11a, CD11b, CD11c, CD18, einem ICAM, VLA-4 und VCAM; einem tumorassoziierten Antigen wie HER2-, HER3- oder HER4-Rezeptor; und Fragmenten der Polypeptide bestehenden Gruppe ausgewählt ist.
  24. Verfahren nach einem der Ansprüche 1 bis 23, wobei die rekombinante Wirtszelle (i) eine eukaryotische Wirtszelle oder (ii) eine prokaryotische Wirtszelle ist.
  25. Verfahren nach Anspruch 24, wobei die eukaryotische Wirtszelle eine Säugetier-Wirtszelle ist und gegebenenfalls eine Chinahamster-Eierstock- (CHO-) Zelle ist oder die prokaryotische Wirtszelle eine Bakterienzelle ist und gegebenenfalls eine E.-coli-Zelle ist.
DE17196350.7T 2007-07-09 2008-07-08 Verhindern der disulfid-bindungsreduktion während der rekombinanten herstellung von polypeptiden Pending DE17196350T1 (de)

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Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2380910T3 (en) 2003-11-05 2015-10-19 Roche Glycart Ag Antigen binding molecules with increased Fc receptor binding affinity and effector function
TWI548747B (zh) 2006-09-13 2016-09-11 艾伯維有限公司 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
SI4365189T1 (sl) 2007-07-09 2025-06-30 F. Hoffmann-La Roche Ag Preprečevanje redukcije disulfidne vezi med rekombinantno proizvodnjo polipeptidov
TWI472339B (zh) 2008-01-30 2015-02-11 Genentech Inc 包含結合至ｈｅｒ２結構域ii之抗體及其酸性變異體的組合物
AR073295A1 (es) 2008-09-16 2010-10-28 Genentech Inc Metodos para tratar la esclerosis multiple progresiva. articulo de fabricacion.
RU2551237C2 (ru) 2008-10-20 2015-05-20 Эббви Инк Инактивация вируса при очистке антител
RU2520838C2 (ru) 2008-10-20 2014-06-27 Эббви Инк Выделение и очистка антител с использованием аффинной хроматографии на основе белка а
EP3828201A1 (de) * 2009-04-30 2021-06-02 Ablynx N.V. Verfahren zur herstellung von domänenantikörpern
CN102482349B (zh) * 2009-06-17 2017-08-25 艾伯维生物医疗股份有限公司 抗vegf抗体和其用途
CA2770690C (en) 2009-08-11 2021-03-09 Genentech, Inc. Production of proteins in glutamine-free cell culture media
AR078161A1 (es) 2009-09-11 2011-10-19 Hoffmann La Roche Formulaciones farmaceuticas muy concentradas de un anticuerpo anti cd20. uso de la formulacion. metodo de tratamiento.
WO2011084496A1 (en) * 2009-12-16 2011-07-14 Abbott Biotherapeutics Corp. Anti-her2 antibodies and their uses
CA2794631A1 (en) * 2010-03-31 2011-10-06 Universite De Geneve Stabilized antibody preparations and uses thereof
TW201302793A (zh) 2010-09-03 2013-01-16 Glaxo Group Ltd 新穎之抗原結合蛋白
CN101974535B (zh) * 2010-11-03 2012-12-19 北京天广实生物技术股份有限公司 新型抗ErbB2人源化抗体MIL12的制备及其应用
US9078878B2 (en) 2010-12-01 2015-07-14 Alderbio Holdings Llc Anti-NGF antibodies that selectively inhibit the association of NGF with TrkA, without affecting the association of NGF with p75
US9884909B2 (en) 2010-12-01 2018-02-06 Alderbio Holdings Llc Anti-NGF compositions and use thereof
US9539324B2 (en) 2010-12-01 2017-01-10 Alderbio Holdings, Llc Methods of preventing inflammation and treating pain using anti-NGF compositions
US8911734B2 (en) 2010-12-01 2014-12-16 Alderbio Holdings Llc Methods of preventing or treating pain using anti-NGF antibodies that selectively inhibit the association of NGF with TrkA, without affecting the association of NGF with p75
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US9067988B2 (en) 2010-12-01 2015-06-30 Alderbio Holdings Llc Methods of preventing or treating pain using anti-NGF antibodies
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN102911265B (zh) * 2011-08-05 2014-02-05 王革 一种人神经生长因子的重组变异体及其制备方法
AR090340A1 (es) * 2012-03-27 2014-11-05 Genentech Inc Operaciones de cosecha mejoradas para proteinas recombinadas
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9650411B2 (en) 2012-08-07 2017-05-16 Kyowa Hakko Kirin Co., Ltd. Method of purifying protein
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
EP2890782A1 (de) 2012-09-02 2015-07-08 AbbVie Inc. Verfahren zur steuerung einer proteinheterogenität
US9976163B2 (en) 2012-10-03 2018-05-22 Kyowa Hakko Kirin Co., Ltd Method for preventing reduction of polypeptide by adding amino acid to culture solution
US20140154255A1 (en) 2012-11-30 2014-06-05 Abbvie Biotherapeutics Inc. Anti-vegf antibodies and their uses
EP2830651A4 (de) 2013-03-12 2015-09-02 Abbvie Inc An humanes tnf-alpha bindende humane antikörper und verfahren zur herstellung davon
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
MY173295A (en) 2013-04-16 2020-01-14 Genentech Inc Pertuzumab variants and evaluation thereof
WO2015009699A1 (en) * 2013-07-15 2015-01-22 Ketter Patrick Methods and compositions to prevent or treat bacterial infections
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
KR20160088930A (ko) * 2013-12-04 2016-07-26 이뮤노젠 아이엔씨 항체 생산용 조성물 및 항체 생성 방법
US9620278B2 (en) * 2014-02-19 2017-04-11 General Electric Company System and method for reducing partial discharge in high voltage planar transformers
US10647777B2 (en) * 2014-05-28 2020-05-12 Genzyme Corporation Methods of controlling the formation of disulfide bonds in protein solutions
US9840553B2 (en) 2014-06-28 2017-12-12 Kodiak Sciences Inc. Dual PDGF/VEGF antagonists
AR103172A1 (es) * 2014-12-22 2017-04-19 Novartis Ag Reducción selectiva de residuos de cisteina en anticuerpos il-17
DK3334760T3 (da) 2015-08-12 2021-04-19 Pfizer Antistofcysteiner med kappe og uden kappe, og deres anvendelse i antistof-lægemiddel-konjugering
ES2995985T3 (en) 2015-08-13 2025-02-11 Amgen Inc Charged depth filtration of antigen-binding proteins
EP3397276A4 (de) 2015-12-30 2019-12-18 Kodiak Sciences Inc. Antikörper und konjugate davon
CA3020839A1 (en) 2016-04-15 2017-10-19 Alder Biopharmaceuticals, Inc. Anti-pacap antibodies and uses thereof
CN109154014A (zh) * 2016-05-10 2019-01-04 豪夫迈·罗氏有限公司 重组产生多肽期间减少三硫键的方法
JP7170542B2 (ja) * 2016-05-10 2022-11-14 メディミューン，エルエルシー タンパク質ジスルフィド結合の還元の防止
SG11201810975UA (en) * 2016-06-10 2019-01-30 Lonza Ag Method for stabilizing proteins
WO2018002036A1 (en) 2016-06-28 2018-01-04 Zaklady Farmaceutyczne Polpharma Sa Recombinant production of monoclonal antibodies
CN106366160B (zh) * 2016-10-11 2019-06-14 厦门大学 基于二硫键精准配对构建富含二硫键多肽分子骨架的方法
EP3541413A2 (de) 2016-11-21 2019-09-25 Polpharma Biologics S.A. Wässrige pharmazeutische formulierungen
CN110662552A (zh) 2016-11-30 2020-01-07 昂科梅德制药有限公司 包含tigit结合剂的癌症治疗方法
EP3564367A4 (de) 2016-12-28 2020-08-12 Kyowa Kirin Co., Ltd. Verfahren zur auswahl von zellen
CN110621341A (zh) * 2017-04-26 2019-12-27 百时美施贵宝公司 使二硫键还原最小化的抗体生产方法
ES2951287T3 (es) * 2017-05-09 2023-10-19 Bristol Myers Squibb Co Método para controlar la formación del color rosa durante la fabricación de anticuerpos
US10913795B2 (en) 2017-05-17 2021-02-09 Immunogen, Inc. Compositions and methods for antibody production
EP3672981A4 (de) * 2017-08-22 2021-09-15 Biogen MA Inc. Verfahren zur reinigung von antikörpern mit aggregaten mit reduziertem hochmolekularem gewicht
TW201923069A (zh) * 2017-10-02 2019-06-16 美商拜耳保健有限責任公司 以亞硒酸鹽防止細胞培養收獲物中雙硫鍵還原之方法
TW201927335A (zh) * 2017-10-02 2019-07-16 美商拜耳保健有限責任公司 防止雙硫鍵於細胞培養收集物中還原之方法
US11447547B1 (en) 2017-12-13 2022-09-20 Amgen Inc. Method of antigen-binding protein production
KR20200140817A (ko) 2018-03-02 2020-12-16 코디악 사이언시스 인코포레이티드 Il-6 항체 그리고 이의 융합 작제물 및 접합체
JP2021521873A (ja) * 2018-05-04 2021-08-30 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ 改変された宿主細胞タンパク質プロファイルを有する操作された細胞
WO2020028616A1 (en) * 2018-08-02 2020-02-06 Lonza Ltd Methods for manufacturing recombinant protein comprising a disulfide bond
KR102699023B1 (ko) 2018-11-08 2024-08-27 서트로 바이오파마, 인크. 산화성 세포질을 갖는 대장균 균주
WO2020201296A1 (en) 2019-04-01 2020-10-08 The Automation Partnership (Cambridge) Ltd. Operation process for a cell cultivation system
CN114144436A (zh) 2019-07-24 2022-03-04 H.隆德贝克有限公司 抗mGluR5抗体及其用途
CN114786731A (zh) 2019-10-10 2022-07-22 科达制药股份有限公司 治疗眼部病症的方法
US12014835B2 (en) 2020-02-19 2024-06-18 Vanderbilt University Methods for evaluating therapeutic benefit of combination therapies
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli
CN113480652B (zh) * 2021-07-30 2023-04-07 成都景泽生物制药有限公司 一种重组cho细胞发酵培养生产重组egfr抗体活性分子的方法
US20240390869A1 (en) 2021-09-21 2024-11-28 Bristol-Myers Squibb Company Methods of controlling the level of dissolved oxygen (do) in a solution comprising a recombinant protein in a storage container
IL312692A (en) 2021-11-16 2024-07-01 Genentech Inc Methods and compositions for the treatment of systemic lupus erythematosus (SLE) with musontuzumab
KR20250069604A (ko) * 2022-09-15 2025-05-19 사노피 단백질 환원 민감성의 결정 방법
AU2024282654A1 (en) 2023-05-30 2025-12-04 Paragon Therapeutics, Inc. Alpha4beta7 integrin antibody compositions and methods of use

Family Cites Families (275)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US524474A (en) * 1894-08-14 Seed-planter
US670534A (en) * 1900-07-27 1901-03-26 Oliver L Carmical Seeding-machine and fertilizer-distributer.
US1369649A (en) * 1920-01-02 1921-02-22 George A Gieseler Pneumatic conveying mechanism
US2062295A (en) * 1932-11-22 1936-12-01 Niagara Sprayer And Chemical C Crop duster
US2171205A (en) * 1937-08-26 1939-08-29 Louis A Zinke Powder distributing attachment for tractors
US2193738A (en) * 1938-05-21 1940-03-12 Nat Conveyors Co Inc Pneumatic conveyer
US2559183A (en) * 1946-12-10 1951-07-03 Lawton L Barnett Transplanter drill set
US2671690A (en) * 1948-02-21 1954-03-09 Ehrenkrook Karl Von Forced air sprayer and duster
US2937049A (en) * 1949-10-18 1960-05-17 Osawa Hirosaburo Method of and an apparatus for carrying coals out of a vertical shaft with the aid of heavy liquid in the coal mine
US2814531A (en) * 1956-04-27 1957-11-26 Clough Equipment Co Balanced pneumatic conveying systems for pulverulent material
US3207560A (en) * 1962-12-26 1965-09-21 Monsanto Co Unloading device
US3197261A (en) * 1964-03-09 1965-07-27 Simpson Herbert Corp System for feeding and transporting materials
US3386474A (en) * 1965-05-26 1968-06-04 Chemagro Corp Diverter valve for finely divided solids
US3515315A (en) * 1966-01-24 1970-06-02 Kidd Designs Ltd Archie Combined seed and fertilizer drills for agricultural use
US3387895A (en) * 1966-12-29 1968-06-11 Combustion Eng Non-clogging splitter unit for dividing the flow of fluid-conveyed material
FR1558490A (de) * 1967-12-27 1969-02-28
US3543704A (en) * 1968-08-05 1970-12-01 Deere & Co Seed planter
US3845702A (en) 1970-04-01 1974-11-05 Mies Filter Prod Inc Apparatus for cooking chicken
DE2440888C3 (de) * 1974-08-27 1978-11-23 Waeschle Maschinenfabrik Gmbh, 7980 Ravensburg Anlage zum aufeinanderfolgenden Beschicken mehrerer, hintereinander an eine pneumatische Förderleitung über Abscheider angeschlossener Entnahmestationen mit Schüttgut
US4049494A (en) 1975-09-04 1977-09-20 The United States Of America As Represented By The Secretary Of Agriculture Vaccine production process
US4036408A (en) * 1975-12-24 1977-07-19 Acf Industries, Incorporated Hopper having means for directing blasts of gas to a discharge outlet or the hopper
US4280419A (en) * 1977-03-21 1981-07-28 International Harvester Company Pneumatic system for conveying granular material
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
FR2413974A1 (fr) 1978-01-06 1979-08-03 David Bernard Sechoir pour feuilles imprimees par serigraphie
US4200412A (en) * 1978-04-20 1980-04-29 Dynamic Air Incorporated Control for pneumatic conveying system
US4264243A (en) * 1979-01-04 1981-04-28 Dundee Cement Company Constant vacuum barge unloading system
US4515893A (en) 1979-04-26 1985-05-07 Ortho Pharmaceutical Corporation Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells
US4244522A (en) * 1979-08-31 1981-01-13 Deere & Company Liquid storage and distribution apparatus for agricultural implements
CA1126587A (en) * 1980-07-11 1982-06-29 Daniel W. Kelm Seed cup assembly
US4413935A (en) * 1981-06-29 1983-11-08 Combustion Engineering, Inc. Flow splitter for dividing a stream of pulverulent material into multiple streams
GB2112506A (en) * 1981-12-30 1983-07-20 Shell Int Research Distributing valve
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
DE3239897A1 (de) * 1982-10-28 1984-05-03 Trützschler GmbH & Co KG, 4050 Mönchengladbach Verfahren und vorrichtung zur foerderung von faserfoermigen stoffen, insbesondere von in luft schwebend gefoerderten textilen faserflocken bei spinnereivorbereitungsmaschinen
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4620948A (en) 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
AU3145184A (en) 1983-08-16 1985-02-21 Zymogenetics Inc. High expression of foreign genes in schizosaccharomyces pombe
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4562779A (en) * 1984-01-26 1986-01-07 Briggs Manufacturing, Inc. Foldable drill implement
DE3420616A1 (de) * 1984-06-02 1985-12-05 Alb. Klein Gmbh & Co Kg, 5241 Niederfischbach Vorrichtung und verfahren zur steuerung eines schleusvorganges fuer ein haufwerk von schuettgutpartikeln
US5672347A (en) 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
US5807715A (en) 1984-08-27 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4572798A (en) 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
US4652630A (en) 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US5523215A (en) 1985-03-28 1996-06-04 Chiron Corporation Enhanced purification and expression of insoluble recombinant proteins
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US4738770A (en) * 1985-11-25 1988-04-19 Cities Service Oil And Gas Corp. Apparatus and method of removal of catalyst blockage from reactor recycle pumps
US5091178A (en) 1986-02-21 1992-02-25 Oncogen Tumor therapy with biologically active anti-tumor antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US4779765A (en) * 1987-04-13 1988-10-25 Deere & Company Fertilizer divider for dual air system
US4793743A (en) * 1987-04-29 1988-12-27 Morris Rod-Weeder Company Ltd. Air seeder purging system
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5091313A (en) 1988-08-05 1992-02-25 Tanox Biosystems, Inc. Antigenic epitopes of IgE present on B cell but not basophil surface
US5720937A (en) 1988-01-12 1998-02-24 Genentech, Inc. In vivo tumor detection assay
US4872785A (en) * 1988-01-22 1989-10-10 Schrage Timothy L Shut-off means for air-actuated planter
US4843983A (en) * 1988-02-02 1989-07-04 Deere & Company Pneumatic manifold quick coupling
US5506126A (en) 1988-02-25 1996-04-09 The General Hospital Corporation Rapid immunoselection cloning method
HUT53672A (en) 1988-02-25 1990-11-28 Gen Hospital Corp Quick immunoselective cloning process
US4861579A (en) 1988-03-17 1989-08-29 American Cyanamid Company Suppression of B-lymphocytes in mammals by administration of anti-B-lymphocyte antibodies
WO1989012463A1 (en) 1988-06-21 1989-12-28 Genentech, Inc. Method and therapeutic compositions for the treatment of myocardial infarction
AU632065B2 (en) 1988-09-23 1992-12-17 Novartis Vaccines And Diagnostics, Inc. Cell culture medium for enhanced cell growth, culture longevity and product expression
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
DK167639B1 (da) * 1988-11-16 1993-12-06 Kongskilde Maskinfabrik As Maskine til saaning af granuleret materiale
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
DE394538T1 (de) 1989-04-28 1991-04-11 Rhein Biotech Ges. Fuer Biotechnologische Prozesse Und Produkte Mbh, 4000 Duesseldorf Hefezellen der schwanniomyces-gattung.
EP0402226A1 (de) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformationsvektoren für Hefe Yarrowia
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
ES2096590T3 (es) 1989-06-29 1997-03-16 Medarex Inc Reactivos biespecificos para la terapia del sida.
FR2649120B1 (fr) 1989-06-30 1994-01-28 Cayla Nouvelle souche et ses mutants de champignons filamenteux, procede de production de proteines recombinantes a l'aide de ladite souche et souches et proteines obtenues selon ce procede
WO1991001367A1 (en) 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
ATE119938T1 (de) 1989-08-21 1995-04-15 Takeda Chemical Industries Ltd Produktion von menschlichen nervenwachstumsfaktoren.
US5033914A (en) * 1989-09-29 1991-07-23 Cyclonaire Corporation High efficiency feeder apparatus for pneumatic conveying lines
SG59931A1 (en) 1989-10-16 1999-02-22 Amgen Inc Stem cell factor
GB2237288A (en) 1989-10-18 1991-05-01 Celltech Ltd Amplification of a heterologous gene in recombinant eukaryotic host cells
DK0463151T3 (da) 1990-01-12 1996-07-01 Cell Genesys Inc Frembringelse af xenogene antistoffer
ATE204902T1 (de) 1990-06-29 2001-09-15 Large Scale Biology Corp Melaninproduktion durch transformierte mikroorganismen
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
JP2938569B2 (ja) 1990-08-29 1999-08-23 ジェンファーム インターナショナル，インコーポレイティド 異種免疫グロブリンを作る方法及びトランスジェニックマウス
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
US5069583A (en) * 1990-10-26 1991-12-03 Caldwell Lawrence G Device for changing flow paths in a pneumatic conveying system
US5252708A (en) 1990-11-23 1993-10-12 Roussel Uclaf Preparation process for a protein containing at least one intramolecular disulphide bridge by oxidation, at a pH of less than 5.0 of corresponding reduced recombinant protein
GB2251249B (en) 1990-12-28 1995-06-21 Mogam Biotech Res Inst High-density medium for animal cell culture
US5494381A (en) * 1991-04-11 1996-02-27 The Young Industries, Inc. Apparatus and method for pneumatically conveying bulk materials
CA2102511A1 (en) 1991-05-14 1992-11-15 Paul J. Higgins Heteroconjugate antibodies for treatment of hiv infection
US5161473A (en) * 1991-05-17 1992-11-10 Deere & Company Seed distribution system and method for a seeding implement
JP4124480B2 (ja) 1991-06-14 2008-07-23 ジェネンテック・インコーポレーテッド 免疫グロブリン変異体
GB9114948D0 (en) 1991-07-11 1991-08-28 Pfizer Ltd Process for preparing sertraline intermediates
ES2296839T3 (es) 1991-08-14 2008-05-01 Genentech Inc. Variantes de inmunoglobulinas para receptores fc-epsilon especificos.
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
WO1993010260A1 (en) 1991-11-21 1993-05-27 The Board Of Trustees Of The Leland Stanford Junior University Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases
ATE503496T1 (de) 1992-02-06 2011-04-15 Novartis Vaccines & Diagnostic Biosynthetisches bindeprotein für tumormarker
US5240355A (en) * 1992-05-22 1993-08-31 Nol-Tec Systems, Inc. Dense phase transporter pneumatic conveying system
JPH08504172A (ja) 1992-06-30 1996-05-07 オンコロジクス，インコーポレイティド 抗−ｅｒｂＢ−２モノクロナール抗体の組み合わせ物及び使用方法
US5456909A (en) 1992-08-07 1995-10-10 T Cell Sciences, Inc. Glycoform fractions of recombinant soluble complement receptor 1 (sCR1) having extended half-lives in vivo
JPH08500017A (ja) * 1992-08-17 1996-01-09 ジェネンテク，インコーポレイテッド 二特異的免疫アドヘジン
DK0656789T3 (da) 1992-08-21 1998-08-24 Genentech Inc Fremgangsmåde til behandling af en LFA-1-medieretforstyrrelse.
US5736137A (en) 1992-11-13 1998-04-07 Idec Pharmaceuticals Corporation Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma
US7744877B2 (en) 1992-11-13 2010-06-29 Biogen Idec Inc. Expression and use of anti-CD20 Antibodies
ATE139900T1 (de) 1992-11-13 1996-07-15 Idec Pharma Corp Therapeutische verwendung von chimerischen und markierten antikörper gegen menschlichen b lymphozyt beschränkter differenzierung antigen für die behandlung von b-zell-lymphoma
US5581476A (en) 1993-01-28 1996-12-03 Amgen Inc. Computer-based methods and articles of manufacture for preparing G-CSF analogs
US5379706A (en) * 1993-04-07 1995-01-10 Agco Corporation Seed distribution system for planters and drills
AU5752594A (en) * 1993-04-20 1994-11-03 Deere & Company Air separation tube for a pneumatic delivery system
US5407305A (en) * 1993-04-29 1995-04-18 Semco, Inc. Continuous dense phase conveying method utilizing high pressure gas at predetermined gas pressures within a conveying pipe
US5417972A (en) 1993-08-02 1995-05-23 The Board Of Trustees Of The Leland Stanford Junior University Method of killing B-cells in a complement independent and an ADCC independent manner using antibodies which specifically bind CDIM
US5663304A (en) 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5595721A (en) 1993-09-16 1997-01-21 Coulter Pharmaceutical, Inc. Radioimmunotherapy of lymphoma using anti-CD20
JP3507852B2 (ja) 1993-12-10 2004-03-15 ジェネンテク，インコーポレイテッド アレルギー診断法および抗アレルギー性治療剤のスクリーニング法
WO1995019181A1 (en) 1994-01-18 1995-07-20 Genentech, Inc. A METHOD OF TREATMENT OF PARASITIC INFECTION USING IgE ANTAGONISTS
CA2181787A1 (en) 1994-03-03 1995-09-08 Claire M. Doerschuk Anti-il-8 monoclonal antibodies for treatment of inflammatory disorders
GB9413127D0 (en) 1994-06-30 1994-08-24 Philips Electronics Uk Ltd Data processing apparatus
US5639635A (en) 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5575225A (en) * 1995-02-27 1996-11-19 Unverferth Manufacturing Company, Inc. Air-powered apparatus for filling grain drills and method of using same
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
IL117645A (en) 1995-03-30 2005-08-31 Genentech Inc Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration
DE19513676A1 (de) * 1995-04-11 1996-10-17 Behringwerke Ag Cytoplasmatische Expression von Antikörpern, Antikörperfragmenten und Antikörperfragmentfusionsmolekülen in E.coli
DE69637481T2 (de) 1995-04-27 2009-04-09 Amgen Fremont Inc. Aus immunisierten Xenomäusen stammende menschliche Antikörper gegen IL-8
AU2466895A (en) 1995-04-28 1996-11-18 Abgenix, Inc. Human antibodies derived from immunized xenomice
CA2221234A1 (en) * 1995-05-15 1996-11-21 Cedars-Sinai Medical Center Compositions and methods for inhibiting xenograft rejection
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
US5830761A (en) 1995-06-07 1998-11-03 Genetics Institute, Inc. Medium and methods for culturing mammalian cho cells
JPH11507535A (ja) 1995-06-07 1999-07-06 イムクローン システムズ インコーポレイテッド 腫瘍の成長を抑制する抗体および抗体フラグメント類
US5783186A (en) 1995-12-05 1998-07-21 Amgen Inc. Antibody-induced apoptosis
US5854067A (en) * 1996-01-19 1998-12-29 Board Of Regents, The University Of Texas System Hexokinase inhibitors
EP0877626B1 (de) 1996-01-23 2002-08-28 Genentech, Inc. Antikörper gegen cd 18 zur verwendung gegen gehirnschlag
US5813801A (en) * 1996-06-28 1998-09-29 Mac Equipment, Inc. Dense phase particulate conveying system and method with continuous air leakage management
US5714388A (en) 1996-08-14 1998-02-03 Bayer Corporation Apparatus and method for detecting chemiluminescent light
US7147851B1 (en) 1996-08-15 2006-12-12 Millennium Pharmaceuticals, Inc. Humanized immunoglobulin reactive with α4β7 integrin
US5749682A (en) * 1996-09-10 1998-05-12 Renfro Corporation Article collection and distribution system
US5912327A (en) 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
AU732447B2 (en) 1996-10-24 2001-04-26 Vanderbilt University Gene delivery and expression in areas inaccessible to direct protein delivery
NZ335207A (en) 1996-11-15 2000-09-29 Genentech Inc Process to isolate recombinant human neurotrophin using hydrophobic chromatography resin
DK1900751T3 (da) * 1996-11-27 2010-04-19 Genentech Inc Affinitetsoprensning af et polypeptid på Protein A-matrix
ATE267257T1 (de) 1996-11-27 2004-06-15 Genentech Inc Humanisierte anti-koerper gegen cd11a
US6037454A (en) 1996-11-27 2000-03-14 Genentech, Inc. Humanized anti-CD11a antibodies
CA2273194C (en) 1996-12-03 2011-02-01 Abgenix, Inc. Transgenic mammals having human ig loci including plural vh and vk regions and antibodies produced therefrom
US6306393B1 (en) 1997-03-24 2001-10-23 Immunomedics, Inc. Immunotherapy of B-cell malignancies using anti-CD22 antibodies
US20020032315A1 (en) * 1997-08-06 2002-03-14 Manuel Baca Anti-vegf antibodies
NZ500078A (en) 1997-04-07 2001-10-26 Genentech Inc Humanized anti-VEGF antibodies and their use in inhibiting VEGF-induced angiogenesis in mammals
DE69838249T3 (de) 1997-05-15 2012-01-19 Genentech, Inc. Anti-apo-2 antikörper
WO1998056418A1 (en) 1997-06-13 1998-12-17 Genentech, Inc. Stabilized antibody formulation
US6171586B1 (en) 1997-06-13 2001-01-09 Genentech, Inc. Antibody formulation
US5927217A (en) * 1997-06-16 1999-07-27 Conserva Pak Seeding Systems Metering particulate material
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
ES2244066T3 (es) 1997-06-24 2005-12-01 Genentech, Inc. Procedimiento y composiciones de glicoproteinas galactosiladas.
US5994511A (en) 1997-07-02 1999-11-30 Genentech, Inc. Anti-IgE antibodies and methods of improving polypeptides
AU8296098A (en) 1997-07-08 1999-02-08 Board Of Regents, The University Of Texas System Compositions and methods for homoconjugates of antibodies which induce growth arrest or apoptosis of tumor cells
US5878679A (en) * 1997-08-18 1999-03-09 Deere & Company Product disconnect for metering device
WO1999022764A1 (en) 1997-10-31 1999-05-14 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
KR100253916B1 (ko) 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
AU760396B2 (en) 1998-01-30 2003-05-15 Lorus Therapeutics Inc. Oligonucleotide sequences complementary to thioredoxin or thioredoxin reductase genes and methods of using same to modulate cell growth
ATE375365T1 (de) 1998-04-02 2007-10-15 Genentech Inc Antikörper varianten und fragmente davon
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
US6528624B1 (en) 1998-04-02 2003-03-04 Genentech, Inc. Polypeptide variants
US6242195B1 (en) 1998-04-02 2001-06-05 Genentech, Inc. Methods for determining binding of an analyte to a receptor
PT1071700E (pt) 1998-04-20 2010-04-23 Glycart Biotechnology Ag Modificação por glicosilação de anticorpos para melhorar a citotoxicidade celular dependente de anticorpos
CA2326389C (en) 1998-04-21 2007-01-23 Micromet Gesellschaft Fur Biomedizinische Forschung Mbh Novel cd19xcd3 specific polypeptides and uses thereof
US6047652A (en) * 1998-05-12 2000-04-11 Case Corporation Seed planter distribution system
EP1241250A3 (de) 1998-05-15 2003-03-19 Genentech, Inc. Menschliches Dehydrogenase-ähnliches Protein und dafür kodierende Nukleinsäure
EP1946775A3 (de) 1998-08-11 2008-08-06 Biogen Idec Inc. Kombinationstherapien für B-Zellen-Lymphome mit Verabreichung eines Anti-CD20-Antikörpers
AU739394B2 (en) 1998-08-27 2001-10-11 Lg Chemical Limited Process for the preparation of active somatotropin from inclusion bodies
CA2343313A1 (en) * 1998-09-14 2000-03-23 Lars Ostergaard Pedersen A method of producing a functional immunoglobulin superfamily protein
US6224866B1 (en) 1998-10-07 2001-05-01 Biocrystal Ltd. Immunotherapy of B cell involvement in progression of solid, nonlymphoid tumors
US6298797B1 (en) * 1998-11-05 2001-10-09 Flexi-Coil Ltd. Nurse inductor apparatus for air seeders
CA2350058C (en) 1998-11-09 2015-10-13 Idec Pharmaceuticals Corporation Treatment of hematologic malignancies associated with circulating tumor cells using chimeric anti-cd20 antibody
DK1131096T3 (da) 1998-11-09 2010-05-17 Biogen Idec Inc Anti-CD20-antistofbehandling af patienter, der modtager knoglemarvs- eller periferisk blodstamcelletransplantationer
EP1141024B1 (de) 1999-01-15 2018-08-08 Genentech, Inc. POLYPEPTID MIT EINER VARIANTE DER HUMANEN IgG1 Fc REGION
US6897044B1 (en) 1999-01-28 2005-05-24 Biogen Idec, Inc. Production of tetravalent antibodies
US6383276B1 (en) 1999-03-12 2002-05-07 Fuji Photo Film Co., Ltd. Azomethine compound and oily magenta ink
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
CA2904259C (en) 1999-05-07 2016-11-15 Genentech, Inc. Use of rituximab to treat vasculitis
US6946129B1 (en) 1999-06-08 2005-09-20 Seattle Genetics, Inc. Recombinant anti-CD40 antibody and uses thereof
JP4286483B2 (ja) 1999-06-09 2009-07-01 イムノメディクス， インコーポレイテッド Ｂ細胞をターゲットとする抗体を使用する自己免疫疾患に対する免疫療法
ITMI991299A1 (it) 1999-06-11 2000-12-11 Consiglio Nazionale Ricerche Uso di anticorpi contro antigeni di superficie per il trattamento della malattia trapianto contro ospite
DE19930748C2 (de) 1999-07-02 2001-05-17 Infineon Technologies Ag Verfahren zur Herstellung von EEPROM- und DRAM-Grabenspeicherzellbereichen auf einem Chip
HUP0202238A3 (en) 1999-07-12 2004-05-28 Idec Pharmaceuticals Inc San D Blocking immune response to a foreign antigen using an antagonist which binds to cd20
JP3833533B2 (ja) 1999-07-30 2006-10-11 ジェネンテック・インコーポレーテッド 荷電濾過膜とその用途
US6451284B1 (en) 1999-08-11 2002-09-17 Idec Pharmaceuticals Corporation Clinical parameters for determining hematologic toxicity prior to radioimmunotheraphy
US8557244B1 (en) 1999-08-11 2013-10-15 Biogen Idec Inc. Treatment of aggressive non-Hodgkins lymphoma with anti-CD20 antibody
JP2003513012A (ja) 1999-08-11 2003-04-08 アイデック ファーマスーティカルズ コーポレイション 抗ｃｄ２０抗体による骨髄病変を伴う非ホジキンリンパ腫を有する患者の治療
AU6929100A (en) 1999-08-23 2001-03-19 Biocrystal Limited Methods and compositions for immunotherapy of b cell involvement in promotion ofa disease condition comprising multiple sclerosis
AUPQ296799A0 (en) 1999-09-20 1999-10-14 Unisearch Limited A cell-membrane impermeable trivalent organoarsenical derivative and use thereof
US6872563B1 (en) 1999-10-05 2005-03-29 President And Fellows Of Harvard College Compositions and methods for production of disulfide bond containing proteins in host cells
AU778199B2 (en) 1999-10-29 2004-11-25 Genentech Inc. Anti-prostate stem cell antigen (PSCA) antibody compositions and methods of use
US20020006404A1 (en) 1999-11-08 2002-01-17 Idec Pharmaceuticals Corporation Treatment of cell malignancies using combination of B cell depleting antibody and immune modulating antibody related applications
WO2002004021A1 (en) 2000-07-12 2002-01-17 Idec Pharmaceuticals Corporation Treatment of b cell malignancies using combination of b cell depleting antibody and immune modulating antibody related applications
US6808902B1 (en) 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules
US6426208B1 (en) 1999-11-12 2002-07-30 Harbor-Ucla Research And Education Institute Recombinant α-L-iduronidase, methods for producing and purifying the same and methods for treating diseases caused by deficiencies thereof
EP1242610A1 (de) 1999-12-22 2002-09-25 Novo Nordisk A/S Verfahren zur extraktion und rückfaltung von polypeptiden, deren cystinbrücken nicht korrekt verknüpft vorliegen
US20030185796A1 (en) 2000-03-24 2003-10-02 Chiron Corporation Methods of therapy for non-hodgkin's lymphoma
EP1267927A1 (de) 2000-03-24 2003-01-02 Chiron Corporation Verfahren zur behandlung von lymphomen vom typ non-hodgkin unter verwendung einer kombination eines antikörpers gegen cd20 und interleukin-2
EP1283722A1 (de) 2000-03-31 2003-02-19 Idec Pharmaceuticals Corporation Kombinierte verwendung von anti-zytokin-antikörpern oder antagonisten und anti-cd20 zur behandlung von b-zell-lymphomen
DK2857516T3 (en) 2000-04-11 2017-08-07 Genentech Inc Multivalent antibodies and uses thereof
EP1286692A4 (de) 2000-04-25 2004-11-17 Idec Pharma Corp Intrathekale verabreichung von rituximab zur behandlung von lymphomen des zenralen nervensystems
US20030009301A1 (en) 2000-05-30 2003-01-09 M.B. Anand Integrated utility meter-reading, billing, payment and usage management system
JP2004512262A (ja) 2000-06-20 2004-04-22 アイデック　ファーマスーティカルズ　コーポレイション 非放射性抗ｃｄ２０抗体／放射標識抗ｃｄ２２抗体の組合せ
SI1296714T1 (sl) 2000-06-22 2010-01-29 S For Entpr University Of Iowa Kombinacija CpG-ja in protiteles, usmerjenih proti CD19, CD20, CD22 ali CD40 za zdravljenje ali preprečevanje raka
US6548276B2 (en) 2000-09-06 2003-04-15 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
US7041479B2 (en) * 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
MXPA03002262A (es) 2000-09-18 2003-10-15 Idec Pharma Corp Terapia de combinacion para tratamiento de enfermedades autoinmunes usando una combinacion de anticuerpos inmunorreguladores/supresores de celulas b.
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
WO2002034790A1 (en) 2000-10-20 2002-05-02 Idec Pharmaceuticals Corporation Variant igg3 rituxan r and therapeutic use thereof
US20070292411A1 (en) * 2000-11-08 2007-12-20 Human Genome Sciences, Inc. Antibodies That Immunospecifically Bind to TRAIL Receptors
KR20030091978A (ko) 2001-01-29 2003-12-03 아이덱 파마슈티칼즈 코포레이션 변형된 항체 및 사용 방법
AU2002327164A1 (en) 2001-01-29 2002-12-09 Idec Pharmaceuticals Corporation Engineered tetravalent antibodies and methods of use
US20030103971A1 (en) 2001-11-09 2003-06-05 Kandasamy Hariharan Immunoregulatory antibodies and uses thereof
US7157557B2 (en) 2001-02-23 2007-01-02 Immunex Corporation Increased recovery of active proteins
JP4391086B2 (ja) * 2001-03-09 2009-12-24 中外製薬株式会社 タンパク質精製方法
US20030040095A1 (en) 2001-03-16 2003-02-27 Achille Arini Method for the production of pharmaceutically active recombinant proteins
ES2364816T3 (es) 2001-04-02 2011-09-14 Genentech, Inc. Terapia de combinación.
JP2005500018A (ja) 2001-04-02 2005-01-06 アイデック ファーマスーティカルズ コーポレイション ＧｎＴＩＩＩと同時発現する組換え抗体
WO2003061694A1 (en) 2001-05-10 2003-07-31 Seattle Genetics, Inc. Immunosuppression of the humoral immune response by anti-cd20 antibodies
EP1404813A4 (de) 2001-06-13 2004-11-24 Genentech Inc Verfahren zur kultivierung tierischer zellen und polypeptidproduktion in tierischen zellen
EP1404366A4 (de) 2001-06-14 2006-06-07 Intermune Inc Kombinationstherapie mit gamma-interferon und b-zellspezifischen antikörpern
US7321026B2 (en) 2001-06-27 2008-01-22 Skytech Technology Limited Framework-patched immunoglobulins
EP1423510A4 (de) 2001-08-03 2005-06-01 Glycart Biotechnology Ag Antikörperglykosylierungsvarianten mit erhöhter antikörperabhängiger zellulärer zytotoxizität
US7718387B2 (en) 2001-09-20 2010-05-18 Board Of Regents, The University Of Texas System Measuring circulating therapeutic antibody, antigen and antigen/antibody complexes using ELISA assays
WO2003029442A1 (en) 2001-10-02 2003-04-10 Novo Nordisk Health Care Ag Method for production of recombinant proteins in eukaryote cells
HUP0600342A3 (en) 2001-10-25 2011-03-28 Genentech Inc Glycoprotein compositions
US6852680B2 (en) 2001-10-26 2005-02-08 Ethyl Corporation Dithiocarbamates containing alkylthio and hydroxy substituents
US20060040348A1 (en) 2001-11-13 2006-02-23 Minmin Qin Methods for producing and purifying recombinant alpha-L-iduronidase
EP1463524A4 (de) 2001-12-07 2005-02-09 Chiron Corp Verfahren oder therapie für nicht-hodgkin-lymphom
GB0130955D0 (en) 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
EP1572902B1 (de) 2002-02-01 2014-06-11 Life Technologies Corporation HOCHWIRKSAME siRNAS ZUR REDUZIERUNG DER EXPRESSION VON ZIELGENEN
AU2003208415B2 (en) 2002-02-14 2009-05-28 Immunomedics, Inc. Anti-CD20 antibodies and fusion proteins thereof and methods of use
CN100516194C (zh) * 2002-03-23 2009-07-22 研究发展基金会 细菌中含有多个二硫键的蛋白质的分泌及其用途
CN102911987B (zh) 2002-04-09 2015-09-30 协和发酵麒麟株式会社 基因组被修饰的细胞
CA2481837A1 (en) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Production process for antibody composition
US20060234226A1 (en) 2002-04-26 2006-10-19 Fahner Robert L Non-affinity purification of proteins
US20030219818A1 (en) 2002-05-10 2003-11-27 Bohen Sean P. Methods and compositions for determining neoplastic disease responsiveness to antibody therapy
US20040029229A1 (en) 2002-05-20 2004-02-12 Reeves Philip J. High level protein expression system
AU2002345829A1 (en) 2002-06-24 2004-01-06 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
GB0216648D0 (en) 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
US7189811B2 (en) 2002-09-06 2007-03-13 National Institute Of Immunology Process for solubilization of recombinant proteins expressed as inclusion body
EP2261230B1 (de) * 2002-09-11 2017-05-10 Chugai Seiyaku Kabushiki Kaisha Verfahren zur Proteinreinigung
US20040138428A1 (en) 2002-10-18 2004-07-15 Gerardo Zapata System and method for cleaving antibodies
MXPA05007378A (es) 2003-01-09 2005-11-23 Genentech Inc Purificacion de polipeptidos.
JP4992029B2 (ja) 2003-01-31 2012-08-08 エム・エス・ディー・オス・ベー・フェー 無酸素条件でのタンパク質の単離方法
AU2004210088A1 (en) 2003-02-06 2004-08-19 Micromet Ag Trimeric polypeptide construct to induce an enduring T cell response
ES2786568T3 (es) 2003-07-28 2020-10-13 Genentech Inc Reducción de la lixiviación de la proteína A durante la cromatografía de afinidad con proteína A
ES2565077T3 (es) 2003-10-10 2016-03-31 Novo Nordisk Health Care Ag Método para producción en gran escala de un polipéptido en células eucariotas
ME01218B (me) 2004-03-01 2013-06-20 Ares Trading Sa Upotreba podloge za ćelijsku strukturu bez sadrzaja seruma za produkciju il-18bp u ćelijama sisara
NZ553500A (en) 2004-09-23 2009-11-27 Genentech Inc Genentech Inc Cysteine engineered antibodies and conjugates withCysteine engineered antibodies and conjugates with a free cysteine amino acid in the heavy chain a free cysteine amino acid in the heavy chain
US7435804B2 (en) 2004-10-19 2008-10-14 Phage Biotechnology, Inc. Method for obtaining single chain antibodies to human interferon α2b
EP1805205A1 (de) 2004-10-22 2007-07-11 Amgen Inc. Verfahren zur rückfaltung von polypeptiden
AU2005299716B2 (en) 2004-10-22 2012-09-06 Amgen Inc. Methods for refolding of recombinant antibodies
GB0425534D0 (en) 2004-11-19 2004-12-22 Celltech R&D Ltd Process for obtaining antibodies
JP2006186521A (ja) 2004-12-27 2006-07-13 Leader Electronics Corp チューナのビットエラーレート（ｂｅｒ）測定の方法および装置
US8029783B2 (en) 2005-02-02 2011-10-04 Genentech, Inc. DR5 antibodies and articles of manufacture containing same
DOP2006000029A (es) 2005-02-07 2006-08-15 Genentech Inc Antibody variants and uses thereof. (variantes de un anticuerpo y usos de las mismas)
AU2006318539B2 (en) 2005-11-23 2012-09-13 Genentech, Inc. Methods and compositions related to B cell assays
WO2007103521A2 (en) 2006-03-07 2007-09-13 Novartis Ag Preventing norvaline and norleucine misincorporation in recombinant proteins
TWI548747B (zh) 2006-09-13 2016-09-11 艾伯維有限公司 細胞培養改良
WO2008127305A2 (en) 2006-11-01 2008-10-23 Biogen Idec Ma Inc. Method of isolating biomacromolecules using low ph and divalent cations
US8691534B2 (en) 2006-12-29 2014-04-08 Celgene Corporation Preparation of romidepsin
US8467449B2 (en) * 2007-01-08 2013-06-18 Qualcomm Incorporated CAVLC enhancements for SVC CGS enhancement layer coding
CA2682170A1 (en) 2007-03-30 2008-10-09 Medimmune, Llc Antibodies with decreased deamidation profiles
WO2008135498A2 (en) 2007-05-04 2008-11-13 Novo Nordisk A/S Prevention of protein degradation in mammalian cell cultures
SI4365189T1 (sl) 2007-07-09 2025-06-30 F. Hoffmann-La Roche Ag Preprečevanje redukcije disulfidne vezi med rekombinantno proizvodnjo polipeptidov
IE20070874A1 (en) 2007-07-12 2009-04-01 Michael Laurence Murphy A hydroalcoholic fat emulsion and emulsifier for use therein
DE202010018640U1 (de) 2009-07-24 2019-11-14 F. Hoffmann-La Roche Ag Rührersystem
KR20160088930A (ko) 2013-12-04 2016-07-26 이뮤노젠 아이엔씨 항체 생산용 조성물 및 항체 생성 방법
US9813410B2 (en) 2014-06-26 2017-11-07 Rakuten, Inc. Information processing apparatus, information processing method, and information processing program
CA3118488A1 (en) 2018-11-05 2020-05-14 Avista Pharma Solutions, Inc. Chemical compounds
IT201900007998A1 (it) 2019-06-04 2020-12-04 Benelli Armi Spa Calcio per arma

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US20250243291A1 (en) 2025-07-31
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