DE1248057B - Process for the production of pure adenosine triphosphate sodium - Google Patents
Process for the production of pure adenosine triphosphate sodiumInfo
- Publication number
- DE1248057B DE1248057B DEV23248A DEV0023248A DE1248057B DE 1248057 B DE1248057 B DE 1248057B DE V23248 A DEV23248 A DE V23248A DE V0023248 A DEV0023248 A DE V0023248A DE 1248057 B DE1248057 B DE 1248057B
- Authority
- DE
- Germany
- Prior art keywords
- adenosine
- adenosine triphosphate
- pure
- triphosphate sodium
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 title claims description 8
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 title claims description 8
- 229910052708 sodium Inorganic materials 0.000 title claims description 7
- 239000011734 sodium Substances 0.000 title claims description 7
- 238000000034 method Methods 0.000 title claims description 6
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N Adenosine Natural products C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 19
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 10
- 229960005305 adenosine Drugs 0.000 claims description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 159000000009 barium salts Chemical class 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000003463 adsorbent Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 150000003014 phosphoric acid esters Chemical class 0.000 claims description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 230000001171 adenosinetriphosphoric effect Effects 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 4
- 229940048102 triphosphoric acid Drugs 0.000 claims description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- 150000008163 sugars Chemical class 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- WZCQRUWWHSTZEM-UHFFFAOYSA-N 1,3-phenylenediamine Chemical compound NC1=CC=CC(N)=C1 WZCQRUWWHSTZEM-UHFFFAOYSA-N 0.000 claims description 2
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 claims description 2
- 238000009833 condensation Methods 0.000 claims description 2
- 230000005494 condensation Effects 0.000 claims description 2
- 230000003544 deproteinization Effects 0.000 claims description 2
- 229940018564 m-phenylenediamine Drugs 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims 1
- -1 adenosine phosphoric acid ester Chemical class 0.000 claims 1
- 235000013325 dietary fiber Nutrition 0.000 claims 1
- 231100000572 poisoning Toxicity 0.000 claims 1
- 230000000607 poisoning effect Effects 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 10
- 239000000047 product Substances 0.000 description 7
- 230000026731 phosphorylation Effects 0.000 description 6
- 238000006366 phosphorylation reaction Methods 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- 229910052788 barium Inorganic materials 0.000 description 2
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- OSJPPGNTCRNQQC-REOHCLBHSA-N 3-phosphoglyceric acid Chemical compound OC(=O)[C@@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-REOHCLBHSA-N 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 1
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
- 229950006790 adenosine phosphate Drugs 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000007859 condensation product Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229940025237 fructose 1,6-diphosphate Drugs 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
Int. Gl.:Int. Eq .:
C07dC07d
DEUTSCHESGERMAN
PATENTAMT Deutsche Kl.: 12 ρ - 7/1Θ PATENT OFFICE German class: 12 ρ - 7 / 1Θ
PATENTSCHRIFTPATENT LETTERING
Nummer:Number:
Aktenzeichen:File number:
Anmeldetag:Registration date:
1 248 057
V23248IVd/12p1 248 057
V23248IVd / 12p
6. November 1962
24. August 1967November 6, 1962
August 24, 1967
7. März 1968March 7, 1968
Die Erfindung betrifft ein Verfahren zur Gewinnung von reinem Adenosintriphosphat-Natrium aus Konzentraten, die aus Adenosin durch Hefegärung in Gegenwart von vergärbaren Zuckern und Phosphatpuffergemisch unter Zusatz von Äther zur Vergiftung der Hefe und dann von Acetaldehyd, anschließende Fällung als Bariumsalz und Enteiweißung der Lösung erhalten worden sind.The invention relates to a method for obtaining pure adenosine triphosphate from sodium Concentrates made from adenosine through yeast fermentation in the presence of fermentable sugars and phosphate buffer mixture with the addition of ether to poison the yeast and then acetaldehyde, subsequent Precipitation as a barium salt and deproteinization of the solution have been obtained.
Es ist bekannt, daß Adenosin durch einen Gärprozeß phosphoryliert werden kann, bei dem als Phosphat- und Kohlehydratquelle anorganische Phosphate und vergärbare Zucker bzw. deren Phosphorsäureester verwendet werden. (»Hop pe-Seylers, Zeitschrift für Physiologische Chemie«, Bd. 250, 1937, S. 155 f., Bd. 251, 1938, S. 258 ff.). Als Endprodukt des Gärprozesses entsteht ein komplexes Gemisch, aus welchem nach Entfernung von Hefe-Zellbestandteilen die phosphorylierten Produkte mit Erdalkalisalzlösungen ausgefällt werden.It is known that adenosine can be phosphorylated by a fermentation process in which as Phosphate and carbohydrate source inorganic phosphates and fermentable sugars or their Phosphoric acid esters can be used. (»Hop pe-Seylers, Zeitschrift für Physiologische Chemie ", Vol. 250, 1937, pp. 155 f., Vol. 251, 1938, pp. 258 ff.). The end product of the fermentation process is a complex mixture, from which after removal of Yeast cell components the phosphorylated products are precipitated with alkaline earth salt solutions.
Eine weitere Reinigung erfolgt nach der Entfernung von Proteinen mittels Trichloressigsäure entweder durch Alkoholfällung oder nach der deutschen Auslegeschrift 1130 785 durch Adsorption an Aktivkohle. Durch diesen Schritt können andere Phosphorsäureester und anorganisches Phosphat abgetrennt werden.A further purification takes place after the removal of proteins either by means of trichloroacetic acid by alcohol precipitation or according to the German Auslegeschrift 1130 785 by adsorption Activated carbon. Through this step, other phosphoric acid esters and inorganic phosphate be separated.
Es ist jedoch schwierig, eine für diesen Verfahrensschritt geeignete Aktivkohle zu finden, da auch Aktivkohlesorten gleicher Bezeichnung innerhalb verschiedener Fabrikationschargen große Unterschiede insofern aufweisen, als die Phosphorylierungsprodukte des Adenosins zwar fest adsorbiert werden, jedoch nicht oder nur zum Teil eluierbar sind. Diese verschieden große Haftfestigkeit ist von Faktoren abhängig, die bei der Herstellung von Aktivkohle nicht beeinflußt werden können.However, it is difficult to find one for this process step to find suitable activated carbon, as there are also activated carbon types of the same name within different manufacturing batches have great differences in that the phosphorylation products of the adenosine are firmly adsorbed, but not or only partially elutable are. These different levels of adhesive strength depend on factors that are involved in the production of Activated charcoal cannot be influenced.
Auf diese Weise wird die durch den biologischen Phosphorylierungsprozeß erreichte gute Ausbeute wieder erheblich herabgesetzt. Es konnte festgestellt werden, daß durchschnitlich 30 bis 40% der Phosphorylierungsprodukte des Adenosins an der Aktivkohle irreversibel adsorbiert werden und verlorengehen. In this way the good yield achieved by the biological phosphorylation process is achieved again significantly reduced. It was found that an average of 30 to 40% of the phosphorylation products of the adenosine are irreversibly adsorbed on the activated carbon and are lost.
Erfindungsgemäß wurde festgestellt, daß es möglich ist, diese Nachteile völlig zu vermeiden, indem das enteiweißte Konzentrat auf eine Säule eines durch Kondensation von m-Pheny!endiamin und Formaldehyd erhaltenen Adsorptionsmittels gebracht und nach Auswaschen von Orthophosphat, anderen Phosphorsäureestern sowie weiteren inaktiven BaI-laststoffen die Adenosintriphosporsäure mittels wäßrigem ammoniakalischem Alkohol eluiert, in bekann-Auslegetag: According to the invention it has been found that it is possible to completely avoid these disadvantages by the deproteinized concentrate on a column of a by condensation of m-pheny! enediamine and Formaldehyde obtained adsorbent brought and after washing out orthophosphate, others Phosphoric acid esters as well as other inactive ballast substances the adenosine triphosphoric acid by means of aqueous ammoniacal alcohol eluted, in known display day:
Ausgabetag:Issue date:
Patentschrift stimmt mit der Auslegeschrift übereinThe patent specification corresponds to the patent specification
Verfahren zur Gewinnung von reinem
Adenosintriphosphat-NatriumProcess for the extraction of pure
Adenosine triphosphate sodium
Patentiert für:Patented for:
VEB Arzneimittelwerk Dresden,
Radebeul 1, Wilhelm-Pieck-Str. 35VEB Arzneimittelwerk Dresden,
Radebeul 1, Wilhelm-Pieck-Str. 35
Als Erfinder benannt:Named as inventor:
Dr.-Ing. Gerhardt Schäfer, RadebeulDr.-Ing. Gerhardt Schäfer, Radebeul
ter Weise als Bariumsalz ausgefällt und zu reinem Adenosintriphosphat-Natrium aufgearbeitet wird.ter way is precipitated as a barium salt and worked up to pure adenosine triphosphate sodium.
Es war überraschend, daß ein Allgemeinadsorber selektiv die Nucleotide adsorbieren würde, während die große Zahl der in der von Zeil- und Eiweißstoffen befreiten Gärlösung noch vorhandenen Stoffwechselprodukte der Hefe, wie 3-Phosphoglycerinsäure, Fruktose-l,6-diphosphat, Glukose-6-phosphat usw., nicht adsorbiert werden, da sich die erwähnten Adsorptionsmittel durch ihren positiven Ladungssinn grundsätzlich von den negativen geladenen Aktivkohlen unterscheiden.It was surprising that a general adsorber would selectively adsorb the nucleotides while the large number of metabolic products still present in the fermentation solution freed from cell and protein substances of yeast, such as 3-phosphoglyceric acid, fructose-1,6-diphosphate, glucose-6-phosphate etc., are not adsorbed, since the adsorbents mentioned are fundamentally different from the negatively charged ones due to their positive sense of charge Distinguish activated carbons.
Die Phosphorylierungsprodukte des Adenosins werden von dem genannten Adsorbens festgehalten. Nach · Entfernung von Orthophosphat und anderen Phosphorsäureestern durch Auswaschen mit verdünnter Salzsäure kann die Adenosintriphosphorsäure durch Elution mit verdünntem ammoniakalischem Methanol ohne Verlust gewonnen und wie oben angegeben in das Adenosintriphosphat-Natrium übergeführt werden.The phosphorylation products of adenosine are retained by the aforementioned adsorbent. After · removal of orthophosphate and other phosphoric acid esters by washing out with dilute Hydrochloric acid can form adenosine triphosphoric acid by eluting with dilute ammoniacal Methanol obtained without loss and converted into adenosine triphosphate sodium as indicated above be transferred.
Ein durch Vergärung von 100 g Adenosin mittels äthervergifteter untergäriger Brauereihefe und Acetaldehydzusatz erhaltenes Gemisch von Phosphorylierüngsprodukten des Adenosins wird mit etwa 5 kg Eisstückchen versetzt und von festen Hefezellbestandteilen abzentrifugiert. Nach Ausfällung des Adenylsäuregemisches durch Bariumsalz sowie dessen Lösung werden noch vorhandene Proteine durch Trichloressigsäure gefällt und entfernt. Das enteiweißte Konzentrat wird auf eine Adsorptionssäule gegeben, die mit 5 1 des unter dem Warenzeichen Wofatit E (®) bekannten Kondensationsproduktes aus m-Phenylendiamin und Formaldehyd beschickt wurde. Das Adsorbens wird mit 3 η-Salzsäure aktiviert und anschließend mit bidestilliertem Wasser säurefrei gewaschen.A fermentation of 100 g of adenosine using ether-poisoned bottom-fermented brewer's yeast and the addition of acetaldehyde obtained mixture of phosphorylation products of adenosine is about 5 kg Pieces of ice are added and the solid yeast cell components are centrifuged off. After precipitation of the Adenylic acid mixture with the barium salt and its solution are replaced by the remaining proteins Trichloroacetic acid precipitated and removed. The deproteinized concentrate is placed on an adsorption column given that with 5 l of the condensation product known under the trademark Wofatit E (®) was charged from m-phenylenediamine and formaldehyde. The adsorbent is activated with 3η hydrochloric acid and then washed acid-free with double-distilled water.
809 525/364809 525/364
Die mit den Phosphorylierungsprodukten des Adenosins beladene Säule wird mit 0,01 n-Salzsäure bariumfrei gewaschen. Darauf erfolgt die Elution in der Gegenrichtung zur Adsorption mit einem Gemisch ausThe column loaded with the phosphorylation products of adenosine is treated with 0.01 N hydrochloric acid washed barium free. Elution then takes place in the opposite direction to adsorption with a mixture out
40 Volumteilen 20°/oigem Ammoniak,
60 Volumteilen destilliertem Wasser,
100 Volumteilen 96°/oigem Äthanol.40 parts by volume of 20% ammonia,
60 parts by volume of distilled water,
100 parts by volume of 96% ethanol.
Aus dem Eluat wird mit einem Bariumsalz Adenosintriphosphat-Barium ausgefällt und dieses in bekannter Weise in das Natriumsalz übergeführt. Die Ausbeute an Adenosintriphosphorsäure beträgt 90 bis 100%.The eluate is converted into adenosine triphosphate barium with a barium salt precipitated and this converted into the sodium salt in a known manner. The yield of adenosine triphosphoric acid is 90 until 100%.
Claims (1)
Deutsche Auslegeschrift Nr. 1130 785;
USA.-Patentschrift Nr. 2 700 038;
»Chimia«, Bd. 9, 1955, S. 92;
»Journal of the American Chemical Society«, Bd. 80, 1958, S. 1143 bis 1145.Considered publications:
German Auslegeschrift No. 1130 785;
U.S. Patent No. 2,700,038;
"Chimia", Vol. 9, 1955, p. 92;
Journal of the American Chemical Society, Vol. 80, 1958, pp. 1143-1145.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEV23248A DE1248057B (en) | 1962-11-06 | 1962-11-06 | Process for the production of pure adenosine triphosphate sodium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEV23248A DE1248057B (en) | 1962-11-06 | 1962-11-06 | Process for the production of pure adenosine triphosphate sodium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1248057B true DE1248057B (en) | 1967-08-24 |
Family
ID=7580405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEV23248A Pending DE1248057B (en) | 1962-11-06 | 1962-11-06 | Process for the production of pure adenosine triphosphate sodium |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE1248057B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2700038A (en) * | 1951-09-27 | 1955-01-18 | Pabst Brewing Co | Dibasic salts of adenosine triphosphate and method of preparation |
| DE1130785B (en) * | 1958-03-21 | 1962-06-07 | Takeda Pharmaceutical | Process for the preparation of 5'-nucleotides, nucleosides and their mixtures by hydrolysis of ribonucleic acids, oligoribonucleotides or their mixtures |
-
1962
- 1962-11-06 DE DEV23248A patent/DE1248057B/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2700038A (en) * | 1951-09-27 | 1955-01-18 | Pabst Brewing Co | Dibasic salts of adenosine triphosphate and method of preparation |
| DE1130785B (en) * | 1958-03-21 | 1962-06-07 | Takeda Pharmaceutical | Process for the preparation of 5'-nucleotides, nucleosides and their mixtures by hydrolysis of ribonucleic acids, oligoribonucleotides or their mixtures |
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