CN203759028U - Improved automatic biochemical analyzer - Google Patents

Abstract

The utility model is an improved automatic biochemical analyzer. Outside the circumference of the reaction discs of 2n reaction cups of the analyzer, a first reagent sample adding arm, a sample stirring device, a cleaning device, a photoelectric detection device, and sample adding are distributed counterclockwise. arm, reagent stirring device, and the second reagent loading arm; the reagent disk is located between the two reagent loading arms, and the sample and reagent loading positions, stirring positions A to G are arranged according to the analyzer speed and reagent incubation time. The reaction disk runs alternately when it is stopped, and each operation cycle has 2 sampling cycles. In each sampling cycle, the reaction disk turns m cups, stops, then turns n+1-m cups, and then stops. Add sample or stir when stopped. The cuvette stops at AEBGFDC one by one, adds the first reagent, adds the sample, stirs, adds the second reagent, stirs, collects and submits the detection data calculation results after reaching the incubation time, and cleans. The utility model only needs one reagent disc and a driving device to complete the addition of two reagents, and the incubation time can be adjusted.

Description

Improved automatic biochemical analyzer

technical field

The utility model relates to the technical field of biochemical analysis, in particular to an improved automatic biochemical analyzer.

Background technique

When performing biochemical analysis, it is necessary to inject the sample to be tested (human or animal body fluids, such as blood, urine, etc.) and reagents into the cuvette, mix well, and perform a chemical reaction (ie incubation) for a certain period of time. It usually takes a few minutes to more than ten minutes for a biochemical analysis project to complete the above steps, and a sample to be tested generally needs to be tested for more than a dozen biochemical indicators. An automatic biochemical analyzer was developed for this purpose.

Automatic biochemical analyzer is an indispensable testing equipment for biochemical laboratories and hospital laboratories. Existing automatic biochemical analyzers generally include reagent trays, sample trays and reaction trays. The sample tray has a plurality of sample containers containing samples to be tested, the reagent tray has a plurality of reagent containers containing reaction reagents for testing, and the reaction tray has a plurality of reaction cups. The containers of each disc are arranged at equal intervals on the circumference of the disc, and the sample disc and 1 or 2 reagent discs are located outside the circumference of the reaction disc. Each disc has the same height and is driven by a driving device to rotate. Each tray is also equipped with a loading arm. A stirring device, a photoelectric detection device and a cleaning device are also arranged outside the circumference of the reaction disc. The control center of the analyzer is connected to control the driving device, sample loading arm, stirring device and cleaning device of each plate. The reagent plate, sample plate and reaction plate are coordinated to rotate and stop according to a certain rule, and each reaction cup is stopped in sequence. Each sampling arm in front of the first reagent plate, sample plate, stirring device, second reagent plate, and stirring device respectively draws samples or reagents quantitatively, injects them into the reaction cup, and the stirring device stirs the contents of the cup; In one turn, the photoelectric detection device collects data once for each cuvette, and the collected data is determined by the parameter setting of the test item. The reaction plate has undergone multiple rounds of rotation to reach the incubation time, and the collected data is sent to the control center for calculation and analysis to obtain the indicators of various biochemical analysis items of the sample. Finally, the cuvette stops at the cleaning device for cleaning and prepares for the next round of sample addition and detection.

In the existing automatic biochemical analyzer with a test speed of more than 400Test/h, in order to achieve a high test speed, the layout of the instrument is generally fixed, and the incubation time cannot be adjusted. Furthermore, two reagent discs and their drives are required.

Utility model content

The purpose of this utility model is to design an improved automatic biochemical analyzer, which only uses one reagent disc to configure one driving device, so as to realize the sequential addition of two reagents.

The improved automatic biochemical analyzer designed by the utility model includes a reagent tray, a sample tray and a reaction tray; the sample tray has a plurality of sample containers for containing the samples to be tested, and the reagent tray has a plurality of reagent containers for placing the reaction reagents for the inspection. The tray has multiple cuvettes. The containers of each disc are arranged at equal intervals on the circumference of each disc, and the sample disc and the reagent disc are outside the circumference of the reaction disc. Each disk is connected with a driving device to drive the disk to rotate. The sample and reagent trays are equipped with loading arms. A stirring device, a photoelectric detection device and a cleaning device are also arranged outside the circumference of the reaction disc. The control center of the analyzer is connected to control the driving device of the reaction disk, the sample disk and the reagent disk, the sample adding arm, the stirring device, the photoelectric detection device and the cleaning device. In the biochemical analyzer, a first reagent sampling arm, a sample stirring device, a cleaning device, a photoelectric detection device, a sample sampling arm, a reagent stirring device and a second reagent sampling arm are distributed counterclockwise outside the circumference of the reaction disk. The reagent disk is located between the first reagent sampling arm and the second reagent sampling arm, that is, the two reagent sampling arms respectively draw the first reagent or the second reagent from the corresponding position of the reagent disk, and inject it at the sample loading position. inside the cuvette.

The reaction plate of the biochemical analyzer has 2n reaction cups, where n is an integer multiple of 6. When the sample loading point A of the first reagent sample loading arm is located at the No. 1 cup, the stirring position B of the sample stirring device of the present invention is located at the No. 3 or 5 or No. 7 cup, and C and D are cleaning devices respectively. In order to obtain the maximum incubation time and avoid interference, BC is separated by 3 cups, that is, the position of point C is cup 7 or 9 or 11, and the eighth-stage cleaning or six-stage cleaning is adopted, and the position of point D is It is cup C+2×8 or cup C+2×6; the point E of the sampling position of the sample loading arm is cup E=B+n+1. According to the improved automatic biochemical analyzer, the test speed is V sampling cycles/H, and one sampling cycle is obtained as t=3600/V seconds; the incubation time of the second reagent is T as required by clinical testing. Then the incubation time T1 of the second reagent must meet at least T/t sampling cycles; when the incubation time is up, the analyzer still needs to collect and calculate data and submit the calculation results to the control center, and the time T2 to collect and submit data calculation results is at least 10 plus Sample cycle; that is, the time T _S >T1+T2 for a cuvette to reach point D after adding the second reagent at point G to start cleaning. When point D is cup x, add the first reagent to cup 1 at point A, and the cup reaches point D for cleaning (2n+1-x) sampling cycles; point G is cup y, G=F+m, then the first reagent is added to a cuvette at point A, and the second reagent is added to point G after (2n+m+1-y) sampling periods; then T _S =(2n+1 -x)-(2n+m+1-y), because T1≥T/t sampling period, T2≥10 sampling period, so (2n+1-x)-(2n+m+1-y )>(T/t)+10. That is, y≥x+m+(T/t)+10, and y is an odd number. Point G cannot exceed point A, so 2n>y≥x+m+(T/t)+10. The position of the stirring point F of the reagent stirring device is ym. m=(n/3±2α), α is an integer, and 2<m<n/2. Therefore, the optional value of the m value and the G point is determined according to the incubation time T of the second reagent required by the design. From the above analysis, it can be seen that the larger the position y of point G is, the longer the incubation time of the second reagent is. The incubation period T reaches a maximum value.

In this system, the first reagent loading arm is at point A, and the second reagent loading arm is at point G. The sampling arm takes the first reagent and the second reagent from the same reagent tray. Dots are at least 5 cups apart. Right now

2n-5>y≥x+m+(T/t)+10.

On the basis of satisfying the above-mentioned evaluation range of m and y, according to the size and position of the operating table of the analyzer, the first reagent injection arm, the second reagent injection arm, the sample injection arm, the sample tray and the reagent tray, Adjust and select the values of m and y during mechanical layout.

The use method of the improved automatic biochemical analyzer designed by the utility model is that the control center controls the driving device of the reaction disk, the sample disk and the reagent disk, controls the rotation of each disk, and simultaneously controls the sample loading arm, the first reagent sample loading arm, The second reagent sample adding arm, the stirring device, the photoelectric detection device and the cleaning device are used to add samples, or stir, or detect, or clean the cuvettes stopped at their corresponding positions.

The control center controls the reaction disk to rotate clockwise, and performs periodic motion in the way of stopping and rotating alternately. Each operation cycle, the reaction disk rotates 2n+2 cups, that is, 360 degrees plus 2 cups. Each run cycle consists of two sampling cycles, and each sampling cycle consists of two spin sequences and two stop sequences. In each sampling cycle, the first rotating timing reaction disk rotates m cups, then the first stop timing, the second rotating timing reaction disk rotates n+1-m cups, and then the second stop timing. The reaction disk rotates n+1 cups per sampling cycle. At least one of the sample stirring device and reagent stirring device in each stop sequence performs a stirring action on the cuvette stopped at its stirring position, and at the same time, At least one cuvette that has sample or reagent added to its loading station.

When the automatic biochemical analyzer improved by the utility model is used, a certain reaction cup R rotates and stops at point A of the sample loading position of the first reagent sample loading arm after cleaning, and the first reagent sample loading arm absorbs the first reagent from the reagent disk. , filled into the cuvette R;

In the next N-th sampling cycle, the cuvette R is rotated to stop at the sample loading position E of the sample sampling arm, and the sample sampling arm draws the sample from the sample tray and fills it into the cuvette R, N=n+ m+1-E;

Thereafter, after a sample adding period, the cuvette R stops at the stirring position B of the sample stirring device, and the sample stirring device stirs the mixed liquid in the cuvette R.

In the m-th sampling cycle after that, the cuvette R is rotated to the sampling position G of the second reagent sampling arm. If the test item needs to add a second reagent, then the second reagent sampling arm draws the first The second reagent is filled into the reaction cup R.

Thereafter, in the first stop sequence after the first rotation sequence of the next sample adding cycle, the reaction cup R stops at the stirring position F of the reagent stirring device, and the reagent stirring device will stir the mixed solution in the reaction cup R.

Finally, another G-D-m sampling period is run, during which the data detected by the photoelectric detection of the R cup is submitted to the control center for result calculation, and then the cuvette R is rotated to the cleaning start point D of the cleaning device, and the cleaning procedure is executed. After D-C sampling cycle, the cleaning is completed when reaching point C, and a clean cuvette is provided for the next round of sampling testing, and then C-A sampling cycles are run, and the R cup will return to point A to start the next round of testing.

Compared with the prior art, the advantages of the improved automatic biochemical analyzer of the utility model are: 1. The structure of the analyzer is simplified, and only one reagent disk and a driving device are needed to complete the addition of two reagents; 2. According to different tests For the incubation time of the project, you can adjust the adding point G and stirring point F of the second reagent, thereby adjusting the incubation time.

Description of drawings

Fig. 1 is the structural representation of the improved automatic biochemical analyzer embodiment;

Fig. 2 is a schematic diagram of the working position of the reaction tray of the embodiment of the improved automatic biochemical analyzer.

The numbers in the figure are: 1. Reaction plate, 2. Cleaning device, 3. Sample stirring device, 4. First reagent loading arm, 5. Reagent disk, 6. Second reagent loading arm, 7. Reagent stirring device, 8. Sample tray, 9. Sample loading arm, 10. Photoelectric detection device.

Detailed ways

The embodiment of the improved automatic biochemical analyzer is shown in Fig. 1, including reagent disc 5, sample disc 8 and reaction disc 1; Reaction plate 1 has a plurality of reaction cups as reagent containers for reaction reagents. The containers of each disk are arranged at equal intervals on the circumference of each disk, and the sample disk 8 and the reagent disk 5 are located outside the circumference of the reaction disk 1 . Each disk has a driving device to drive the disk to rotate. The sample tray 8 is equipped with a sample loading arm 9 , and the reagent tray 5 is equipped with a first reagent loading arm 4 and a second reagent loading arm 6 . A sample stirring device 3 , a reagent stirring device 7 , a photoelectric detection device 10 and a cleaning device 2 are also provided outside the circumference of the reaction disk 1 . The control center of the analyzer is connected to control the driving device of the reaction disk 1, the sample disk 8 and the reagent disk 5, the first reagent sample loading arm 4, the second reagent sample loading arm 6, the sample sample loading arm 9, the reagent stirring device 7, the sample Stirring device 3 , photoelectric detection device 10 and cleaning device 2 . The reaction tray 1 of the biochemical analyzer in this example has 120 reaction cups. Outside the circumference of the reaction disk 1, the first reagent sample loading arm 4, the sample stirring device 3, the cleaning device 2, the photoelectric detection device 10, the sample sample loading arm 9, the reagent stirring device 7, the second reagent sample loading Sample arm 6. Point A is the position where the first reagent loading arm 4 adds the first reagent, point B is the position where the sample stirring device 3 stirs the first reagent and the sample, point C is the end position of the cleaning device, point D is the starting position of the cleaning device, and point E is the position where the sample stirring device 3 stirs the first reagent and the sample. Point F is the position where the sample loading arm 9 adds the sample, point F is the position where the reagent stirring device 7 stirs the two reagents and the sample, and point G is the position where the second reagent loading arm 6 adds the second reagent. The reagent disk 5 is between the first reagent sample loading arm 4 and the second reagent sample loading arm 6, and the two reagent sample loading arms 4, 6 can suck the first reagent or the second reagent from the corresponding position of the reagent disk 5, note in the cuvette to its corresponding loading position. When the point A of the first reagent sample adding arm 4 is the No. 1 cup, the stirring position of the sample stirring device 3 in this example is the No. 7 cup; the cleaning device of this example adopts eight-stage cleaning, and the point C is Cup No. 11 is at point D, cup No. 27 is at point D; cup No. 68 is at point E where sample loading arm 9 adds samples. According to the test speed of the improved automatic biochemical analyzer 400 sample addition cycles/H, one sample addition cycle is obtained as t=9 seconds; the clinical test requirement of this example, the incubation time of the second reagent is T=300 seconds. Then the incubation time T1 of the second reagent must satisfy at least T/t≈34 sampling cycles; after the incubation time in this example is reached, the time T2 for collecting and submitting data calculation results is 10 sampling cycles; After adding the second reagent at point D, the time T _S for cleaning at point D is >44. In this example, when point D is cup No. 27, cup No. 1 at point A begins to add the first reagent, and the cup reaches point D for cleaning. After (2n+1-x)=94 sampling cycles; at point G It is the y cup, G=F+m, then a cuvette adds the first reagent at point A, and adds the second reagent at point G after (2n+m+1-y) sampling periods; then it can be seen that T _S =( 2n+1-x)-(2n+m+1-y), so (2n+1-x)-(2n+m+1-y)>44. That is, y≥27+m+44, and y is an odd number. Point G cannot exceed point A, and for the convenience of the installation and operation of the reagent disc 5, there are 2n-5>y≥27+m+44, 115>y≥71+m, which is determined by the stirring position F of the reagent stirring device 7 The location is ym. In this example m=(20±2α), α is an integer, and 2<m<30, m in this example is an even number from 4 to 28, and y is an odd number from 75 to 113.

According to the size and position of the operating table of the analyzer of this example, the first reagent sample loading arm 4, the second reagent sample loading arm 6, the sample sample loading arm 9, the sample tray 8 and the reagent tray 5, when performing mechanical layout Adjust within the above optional values. In this example, m=22 and y=105 are finally taken. That is, in this example, when point A is cup No. 1, point B is cup No. 7 counterclockwise, point C is cup No. 11, point D is cup No. 27, point E is cup No. 68, point F is cup No. 83, and point G is cup No. The point is cup No. 105, as shown in Figure 2.

The above-mentioned embodiments are only specific examples for further specifying the purpose, technical solutions and beneficial effects of the utility model, and the utility model is not limited thereto. All modifications, equivalent replacements, improvements, etc. made within the disclosed scope of the present utility model are included in the protection scope of the present utility model.

Claims (4)

1. An improved automatic biochemical analyzer, comprising a reagent tray (5), a sample tray (8) and a reaction tray (1); the sample tray (8) has a plurality of sample containers for containing samples to be tested, and the reagent tray (5) has A plurality of reagent containers containing reaction reagents for inspection are placed, and the reaction tray (1) has a plurality of reaction cups; the containers of each tray are arranged at equal intervals on the circumference of each tray, and the sample tray (8) and the reagent tray (5) are in the outside the circumference of the reaction disc (1); each disc is connected with a driving device to drive the disc to rotate; the sample disc (8) and the reagent disc (5) are equipped with a sample loading arm; the reaction disc (1) is also provided with a stirring device, A photoelectric detection device (10) and a cleaning device; the control center of the analyzer is connected to control the driving device of the reaction disk (1), the sample disk (8) and the reagent disk (5), and is also connected to control each sample loading arm, stirring device, photoelectric Detection device (10) and cleaning device (2); It is characterized in that: Outside the circumference of the reaction disk (1), the first reagent sample loading arm (4), the sample stirring device (3), the cleaning device (2), the photoelectric detection device (10), and the sample sample loading arm are distributed counterclockwise. (9), reagent stirring device (7), second reagent sample loading arm (6); reagent disc (5) is between the first reagent sample loading arm (4) and the second reagent sample loading arm (6), namely The two reagent sample injection arms (4, 6) suck the first reagent or the second reagent from the corresponding position of the reagent plate (5) respectively, and inject them into the cuvettes at the sample injection positions. 2. The improved automatic biochemical analyzer according to claim 1, characterized in that: The reaction tray (1) has 2n reaction cups, and n is an integer multiple of 6; when point A of the sample loading position of the first reagent sample loading arm (4) is located in the No. 1 cup, the sample stirring device (3 )’s stirring position B point is the No. 3 or No. 5 or No. or cup No. 11, where point D is located in cup C+2×8 or cup C+2×6; the point E of the sample loading arm (9) is located at cup E=B+n+No. 1; according to The automatic biochemical analyzer test speed of this improvement is V sample addition cycles/H, obtains a sample addition cycle and is t=3600/V second; Clinical examination requirement, the incubation time of the second reagent is T; Then the second reagent's The incubation time T1 must meet at least T/t sampling cycles; after the incubation time is reached, the time T2 for collecting and submitting data calculation results is at least 10 sampling cycles; Time to start cleaning at point D T _S >T1+T2; when the position of point D is cup x, add the first reagent to cup 1 at point A, and the reaction cup after adding the first reagent reaches point D for cleaning experience (2n+1-x) sampling cycles; the position of point G is cup y, G=F+m, then add the first reagent at point A to a cuvette and the second reagent at point G after (2n+m +1-y) sampling period, obtain T _S =(2n+1-x)-(2n+m+1-y)>(T/t)+10, that is, y≥x+m+(T/t )+10, while y is an odd number; point G does not exceed point A, so 2n>y≥x+m+(T/t)+10; the stirring position F of the reagent stirring device (7) is located at ym; m=(n/3±2α), α is an integer, and 2<m<n/2. 3. The improved automatic biochemical analyzer according to claim 2, characterized in that: The points A and G are separated by at least 5 cups, that is, 2n-5>y≥x+m+(T/t)+10. 4. The improved automatic biochemical analyzer according to claim 2, characterized in that: On the basis of satisfying the evaluation range of m and y, according to the size of the operating table of the analyzer, the first reagent loading arm (4), the second reagent loading arm (6), the sample loading arm (9), The size and position of the sample tray (8) and the reagent tray (5) are adjusted and selected during the mechanical layout, and the values of m and y are selected.