CN208902733U - Wide-range immuno-chromatography detection device - Google Patents
Wide-range immuno-chromatography detection device Download PDFInfo
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- CN208902733U CN208902733U CN201821634063.2U CN201821634063U CN208902733U CN 208902733 U CN208902733 U CN 208902733U CN 201821634063 U CN201821634063 U CN 201821634063U CN 208902733 U CN208902733 U CN 208902733U
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- 238000001514 detection method Methods 0.000 title claims abstract description 70
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 101
- 238000005259 measurement Methods 0.000 claims abstract description 17
- 238000002347 injection Methods 0.000 claims abstract description 4
- 239000007924 injection Substances 0.000 claims abstract description 4
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims abstract description 3
- 230000003287 optical effect Effects 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 4
- 238000007689 inspection Methods 0.000 claims description 3
- 230000002452 interceptive effect Effects 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000007813 chromatographic assay Methods 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000000020 Nitrocellulose Substances 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000013139 quantization Methods 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model relates to a kind of wide-range immuno-chromatography detection devices, the device of the utility model includes shell, circuit of measurement and control plate, display and prod, it is characterized by: the front of the upper housing is respectively equipped with the display window with display cooperation, the end of shell is equipped with and is inserted into for prod, the injection port that is connected to test strips；The prod is made of a suction batten, upper cover, pedestal, a high concentration test strips and a low concentration test strips, the upper surface of the upper cover is equipped with detection window, it is convex that triangle light splitting is equipped in the detection window, constitute two test sections of detection window, respectively high concentration test section and low concentration test section；Two test strips are respectively arranged on the lower section that triangle is divided the convex left and right sides, and are located on the direction for inhaling the flowing of batten tested solution.The utility model detection device two test strips on prod is arranged the method for two critical values, by using same light source to extend the range of linearity of immuno-chromatographic assay technology.
Description
Technical field
The utility model relates to a kind of wide-range immuno-chromatography detection devices.
Background technique
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology risen in recent years, is examined
Off line reason is: special antibody (antigen) is bonded to a certain zone of the nitrocellulose filter of test strips first；Work as test strips
It is dry immerse fluid to be measured (urine or serum) containing nitrocellulose filter one end after, due to capillarity, fluid to be measured
Sample will permeate forward along the film；When fluid to be measured, which infiltrates into solidification, the region of antibody (antigen), phase in fluid to be measured
The antigen answered is specifically bound with the antibody；If dyed using immune colloid gold or immuno-enzymatic, this can be made
Region shows certain color, to realize the immunodiagnosis of specificity.
In the home medical quick diagnosis technology of early stage, the method for generalling use visual inspection, the inspection to immuno-chromatographic test paper strip
The result for surveying color judges.But this judgement varies with each individual, error is larger；If it is very low to encounter measurand content,
It develops the color very shallow；Alternatively, measurand content is very high, develop the color chin-deep, is difficult with the naked eye accurately to determine, can not provide the knot of quantization
Fruit.In this way, will lead to the erroneous judgement to user health situation, or it cannot be accurately tracked by judgement health status and development trend,
To cause mistaken diagnosis.It is accordingly required in particular to which one kind being capable of wide scope, high-precision, the accurate reading immunity-chromatography test strip inspection of energy
Survey the device of result.
ZL200910214016.1 Chinese utility model patent discloses and " a kind of Weigh sensor and reads immune layer
Analyse method and system and its application of test strips ".Although the patent realizes the detection knot of quantitative reading immunity-chromatography test strip
Fruit.But it is above-mentioned there is a problem in that: 1, operating procedure is complicated, and test strips are separately positioned with reading device, need to develop the color
Test strips afterwards are manually placed into reading device, are read out to it；Therefore, the test strips of different measurands are easy mixed
Confuse.2, it is easy to interfere with each other between the detection zone of test strips and the detection light of control area, it is unreliable to read result.
ZL200810060585.0 Chinese utility model patent discloses " a kind of optical analysis reading device ".This is specially
Every test strips of benefit use a light source, are irradiated to the test strips.Therefore, three correspondences are respectively adopted in three test strips
The light source of test strips.That is: 3 light emitting diodes respectively detect the test strips in the first, second, and third region, but should
Device contains only the photodetector in the reflected light in (three regions) on above three survey paper slip that can receive to come.Although
The patent can be realized the testing result of quantitative reading immunity-chromatography test strip；But above-mentioned patent there is a problem in that: 1,
The light source that three different light emitting diodes are constituted, the benchmark and characteristic of work are discrete；To solve the above problems, can only
By stringent device screening, the identical diode light-source of physical characteristic is selected, it is close to reach control device Property comparison
Target；However, the screening of this device, increases production cost.Even if 2, by the screening of device, three light emitting diodes or
More or less discrete discrepancy, and will affect the stability and accuracy of test result.3, single immuno-chromatographic test paper strip is facing
The linear detection range of dividing value is very limited.
ZL201410040407.7 Chinese utility model patent discloses a kind of immuno-chromatography detection device and method.
The detection device of the patent uses translucency immuno-chromatographic test paper strip.The measuring mechanism of the patent is: utilizing nitrocellulose
The perspectivity of reaction marking object respectively detects detection zone and control area from the upper and lower faces of test strips on film,
The optical path cavity of above-mentioned detection zone and the optical path cavity of control area are to be arranged independently of each other.Its main feature is that: completely cut off detection
Interfering with each other between region and control area detection light enhances the reliability of testing result.But due to immunity-chromatography test
The limitation of paper slip working mechanism, a critical value can only be arranged in a test strips, and the range of linearity of detection accuracy is critical
Value is nearby very limited.If critical value setting is higher, have a significant impact to the detection accuracy at low concentration end；If critical
Value setting is relatively low, then has a significant impact to the detection accuracy at high concentration end.In addition, setting due to the detection device and test strips
It is calculated as deserted integrated structure, higher operating costs.
Utility model content
The purpose of this utility model first is that in order to solve above-mentioned various defects, a kind of wide-range immunochromatography detection is provided
Device.
The first purpose of the utility model patent be achieved in that a kind of wide-range immuno-chromatography detection device and its
Prod, including shell, circuit of measurement and control plate, display and prod, the display are set on circuit of measurement and control plate；
It is characterized by: the front of the shell is respectively equipped with the display window with display cooperation；The prod by a suction batten,
One upper cover, a pedestal, a high concentration test strips and a low concentration test strips are constituted, the high concentration test strips and low concentration
The concentration markers difference of test strips, the upper surface of the upper cover are equipped with detection window, and the detection window is interior convex equipped with triangle light splitting,
It will test window and be divided into two test zones, respectively high concentration test section and low concentration test section；One end of the prod
Equipped with the sample introduction end for inhaling batten insertion and being connected to test strips；Two test strips are respectively arranged on triangle light splitting convex left and right two
The lower section of side, and be located on the direction for inhaling the flowing of batten tested solution.
The circuit of measurement and control plate, including a light source, two photodetectors and microcontroller circuit；The light source warp
Triangle is divided the convex detection zone for being incident on two test strips respectively；The photodetector, which is located at two, can receive test strips
Reflected light region after detection.
The light source is LED light emitting diode.
The circuit of measurement and control plate further includes the light shield for preventing optical path signal from interfering with each other.
The detection zone is located at the same face of two test strips.
The photodetector is arranged separately on the left and right sides of light source, since triangle is divided convex and light shield effect, makes
It is mutually indepedent to obtain two detection zones.
Light source and photodetector row on the circuit of measurement and control plate are set in a straight line, and the light source is located at two light
Between detector, the detection window of the light source, light shield and photodetector face prod, the triangle light splitting on the prod
The light source center vertical alignment of convex tip and circuit of measurement and control plate.
The utility model has the advantages that: (1) described two detection zones be respectively provided at the same face of two piece test strips, and two
Photodetector is separately positioned on the two sides of light source, keeps three arranged in a straight line；In this way, with the flow direction of sample introduction fluid to be measured at 90
Angle is spent, and photodetector is enable to receive the incident light of same light source completely.(2) due to using same light source, on prod
Using the detection method of two critical values set by two test strips, come extend immuno-chromatographic assay technology the range of linearity and
The linearity；In this way, therefore expanding effective detection range increases testing result detection accuracy；And it is easy to operate, it shows
Show result quantization and intuitive.(3) test window is convex using triangle light splitting and light shield is used in combination, so that two detection zones
Optical path cavity it is mutually indepedent, avoid and interfere with each other.
Detailed description of the invention:
Fig. 1 are as follows: two test strips of high concentration and low concentration of the utility model correspond to the linear graph of critical value；
Fig. 2 are as follows: the explosive view of the utility model；
Fig. 3 are as follows: the cross-sectional view of the utility model.
Specific embodiment
The working mechanism of the utility model wide-range immuno-chromatography detection device is: by the nitrocellulose to test strips
On film, the discharge rate of the antibody (antigen) of generated specific reaction and the adjustment of concentration, so that the two of high concentration and low concentration
Corresponding to root test strips, the range of linearity of critical value can be docked effectively.As shown in Figure 1, if setting the linear of high concentration test strips
The lower limit value of degree is A, ceiling value B；The lower limit value of the linearity of low concentration test strips is D, ceiling value C；By adjusting two
The concentration and discharge rate of root test strips, so that linear lower bound of the linear ceiling value C of low concentration test strips close to high concentration test strips
Value A.In this way, the AtoB range of linearity of the DtoC range of linearity of low concentration test strips and high concentration test strips is just merged extension
To the range of linearity of DtoB, the detection accuracy of gamut is improved.
To make the structure feature to the utility model and the effect of being reached has a better understanding and awareness, to compared with
Good examples and drawings cooperation, detailed description are as follows:
As shown in Figure 2,3, the wide-range immuno-chromatography detection device of the utility model, including shell 1, circuit of measurement and control
Plate 2, display 3 and prod 4, the shell 1 are made of upper housing 11 and lower case 12.The setting of display 3 is measuring
In control circuit board 3, and cooperate with the display window 13 on 11 surface of upper housing；The side of the shell 1 is equipped with inserts for prod 4
The injection port 14 entered.
The prod 4 is by upper cover 41, the low concentration test strips 44 and one of high concentration test strips 43, one of pedestal 42, one
Root is inhaled batten 40 and is constituted, and the upper cover 41 is equipped with testing window 411, and triangle light splitting convex 412 is equipped among the testing window 411,
For being supplied to high concentration test strips 43 and the identical light source 21 of low concentration test strips 44；Described one end for inhaling batten 40 is through sample introduction
Mouthful insertion after, in prod 4 high concentration test strips 43 and low concentration test strips 44 contact, the other end stays in prod 4
Outside.When detection, the effect that the triangle is divided convex 412 is that optical path is divided into two, and makes 43 He of high concentration test strips
Low concentration test strips are located at two independent optical paths, have both ensured the comparativity of same light source testing result, the property of can refer to, and have subtracted
Few different light sources are because of invalid test result caused by signal source difference；It in turn avoids between two test strips detection zone detection lights
Interfere with each other.
The circuit of measurement and control plate 2 is made of light source 21, two photodetectors 22, microcontroller circuit, the light source 21
For LED semiconductor diode, it is mounted on the centre of detection window, two photodetectors are mounted on the two sides of light source 21.
When in use, fluid to be measured is immersed into the outer end for inhaling batten 40, so that liquid sample flows to height along batten is inhaled respectively
In concentration test strips 43 and low concentration test strips 44.The light source 21 is respectively radiated to high concentration test strips 43 and low concentration test paper
After on item 44, it is re-reflected on two photodetectors 22.By by the photoelectric conversion of photodetector 22, the circuit of measurement and control
After 2 receive current signal, conversion process analysis is carried out, processing analysis result is passed through liquid crystal by the validity of judgement sample detection
Display 2 shows that shown processing analysis result can be quantitative analysis results or the qualitative analysis.
The wide-range immunochromatography detection method of the utility model, includes the following steps:
(1) prod 4 is inserted into the injection port 14 of the detection device of wide-range immunochromatography;
(2) it is on switch, starts detection device;
(3) the suction batten 40 of prod 4 is contacted into fluid to be measured, postpones 5-15 seconds；Make fluid to be measured by inhaling batten 40,
It is flow in high concentration test strips 43 and low concentration test strips 44 respectively;
(4) it firstly, after light source is radiated at the detection zone of high concentration test strips 43 and low concentration test strips 44, is reflected into each
From detection device；Secondly, carrying out photoelectric conversion to above-mentioned two detection device respectively；Third, by above two electric signal point
It is not sent to measurement control panel 2, the validity of judgement sample detection；4th, the microcontroller measured on control panel 2 receives
Current signal carries out conversion process and analysis, and processing analysis result is shown by liquid crystal display;
(5) used prod is popped up, test next time is waited.
Since the detection device of the utility model is by using same light source, to two label same antibodies on prod
(antigen), but the test strips of various concentration are tested.The test strips are by using the method that two critical values are arranged, to extend
The range of linearity of immuno-chromatographic assay technology.The test method of the utility model not only expands effectively detection range, Er Qieti
High measuring accuracy.With the utilization of light source, but also the reference standard of two sections of linearity tests reaches unified, so as to reduce detection
As a result deviation enhances the accuracy and reliability of testing result, the detection of routine immunization chromatograph test strip is overcome, in quilt
The concentration for surveying liquid sample cannot provide the limitation of more accurately testing result when far from critical value.Also avoid routine
In, datum drift and linearity deviation between different light sources lead to unstable/uncertain defect of testing result.
Claims (7)
1. a kind of immuno-chromatography detection device of wide-range, including shell (1), circuit of measurement and control plate (2), display (3) and
Prod (4), the shell (1) are made of upper housing (11) and lower case (12), and the display (3) is set to measurement control electricity
On road plate (2)；It is characterized by: the front of the upper housing (11) is respectively equipped with the display window (13) with display (3) cooperation,
The end of shell (1) is equipped with the injection port (14) being connected to for prod (4) with test strips；The prod (4) is inhaled by one
Batten (40), upper cover (41), pedestal (42), high concentration test strips (43) He Yigen low concentration test strips (44) are constituted, institute
State that high concentration test strips (43) are different with the concentration markers of low concentration test strips (44), the upper surface of the upper cover (41) is equipped with inspection
It surveys window (411), triangle is equipped in the detection window (411) and is divided convex (412), constitutes two test sections of detection window (411),
Respectively high concentration test section and low concentration test section；Two test strips are respectively arranged on triangle and are divided at left and right sides of convex (412)
Lower section, and be located at inhale batten tested solution flowing direction on.
2. a kind of immuno-chromatography detection device of wide-range according to claim 1, it is characterised in that: the measurement control
Circuit board (2), including a light source (21), two photodetectors (22) and microcontroller circuit；The light source (21) is through triangle
Shape is divided convex (412) and is incident on the detection zone in two test strips；The photodetector (22), which is located at two, to be connect
By the reflected light zone position of the detection of test strips.
3. a kind of immuno-chromatography detection device of wide-range according to claim 2, it is characterised in that: the light source
It (21), is LED light emitting diode.
4. a kind of immuno-chromatography detection device of wide-range according to claim 2, it is characterised in that:
The circuit of measurement and control plate (2) further includes the light shield for preventing optical path signal from interfering with each other (23).
5. a kind of immuno-chromatography detection device of wide-range according to claim 2, it is characterised in that: the detection zone
It is located at the same face of two test strips.
6. a kind of immuno-chromatography detection device of wide-range according to claim 2, it is characterised in that: the photodetector
(22), it is arranged separately on the left and right sides of light source (21), since triangle is divided the effect of convex (412), so that two detection zones
Domain is mutually indepedent.
7. a kind of immuno-chromatography detection device of wide-range according to claim 2, it is characterised in that: the measurement control
Light source (21) and photodetector (22) row on circuit board (2) are set in a straight line, and the light source (21) is located at two light detections
Between device (22), the light source (21), light shield (23) and photodetector (22) face prod (4) detection window (411), it is described
Triangle on prod is divided the tip of convex (412) and light source (21) center vertical alignment of circuit of measurement and control plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821634063.2U CN208902733U (en) | 2018-10-09 | 2018-10-09 | Wide-range immuno-chromatography detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821634063.2U CN208902733U (en) | 2018-10-09 | 2018-10-09 | Wide-range immuno-chromatography detection device |
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| Publication Number | Publication Date |
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| CN208902733U true CN208902733U (en) | 2019-05-24 |
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| CN201821634063.2U Expired - Fee Related CN208902733U (en) | 2018-10-09 | 2018-10-09 | Wide-range immuno-chromatography detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109239338A (en) * | 2018-10-09 | 2019-01-18 | 邱华遗 | Wide-range immuno-chromatography detection device and method |
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2018
- 2018-10-09 CN CN201821634063.2U patent/CN208902733U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109239338A (en) * | 2018-10-09 | 2019-01-18 | 邱华遗 | Wide-range immuno-chromatography detection device and method |
| CN109239338B (en) * | 2018-10-09 | 2023-09-08 | 邱华遗 | Wide-range immunochromatography detection device and method |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20190524 |