CN103760339B - Immuno-chromatography detection device and method - Google Patents
Immuno-chromatography detection device and method Download PDFInfo
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- CN103760339B CN103760339B CN201410040407.7A CN201410040407A CN103760339B CN 103760339 B CN103760339 B CN 103760339B CN 201410040407 A CN201410040407 A CN 201410040407A CN 103760339 B CN103760339 B CN 103760339B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to a kind of immuno-chromatography detection device and method, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the detection line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of the light path cavity of surveyed area and control area separate.Pick-up unit of the present invention has completely cut off surveyed area and control area completely and has detected mutual interference between light, enhances the reliability of testing result, and simple to operate, and display result quantizes and directly perceived.
Description
Technical field
The present invention relates to a kind of immuno-chromatography detection device and method.
Background technology
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology of rising in recent years, its principle is a certain zone special antibody being first fixed on nitrocellulose filter, after sample (urine or serum) is immersed in cellulose nitrate one end of this drying, due to capillarity, sample will move forward along this film, when moving to the region being fixed with antibody, in sample corresponding antigen namely with this antibody generation specific binding, if this region can be made to show certain color with immune colloid gold or Immunoperoxidase Staining, thus realize specific immunodiagnosis.
In China's home medical quick diagnosis field, usually the testing result of method to immuno-chromatographic test paper strip of visual inspection is adopted to judge at present, but this judgement varies with each individual, simultaneously to run into measurand content very low, develop the color very shallow, be difficult to the naked eye result of determination, cause the mistaken diagnosis that the erroneous judgement of user health situation is caused.Therefore, special needs can the device of accurate reading immunity-chromatography test strip testing result.
Number of patent application be 200910214016.1 Chinese invention patent application disclose method and system and the application thereof of a kind of Weigh sensor and reading immunity-chromatography test strip, although achieve quantitative reading immunity-chromatography test strip testing result, but there are the following problems: 1, operation steps is complicated, test strips is separated setting with reading device, need the test strips after by colour developing to be manually placed in reading device to read, easily obscure between different test strips.2, easily interference mutually between the surveyed area of test strips and the detection light of control area, reads result unreliable.
Summary of the invention
The object of the invention is, in order to solve the problem, to provide a kind of immuno-chromatography detection device.
For achieving the above object, technical scheme of the present invention is as follows:
A kind of immuno-chromatography detection device, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of the light path cavity of surveyed area and control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector are connected with microcontroller respectively with power circuit, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area.
Preferably, described control area light path cavity is arranged on the top of control area, described surveyed area light path cavity is arranged on the below of surveyed area, or described control area light path cavity is arranged on the below of control area, and described surveyed area light path cavity is arranged on the top of surveyed area.
Preferably, described circuit of measurement and control also comprises the testing circuit for detecting sample validity, and described control area light source is connected with microcontroller respectively by described testing circuit with control area photodetector.
Preferably, described test strips injection port is provided with the suction lining bar be communicated with test strips.
Preferably, described display is LCDs.
Preferably, the sample photodetector for automatically controlling testing circuit is provided with in described housing.
Preferably, described pick-up unit also comprises lid, and described lid and shell end are removably connected to form airtight cavity, and test strips injection port is sealed in above-mentioned cavity.
Preferably, the profile of described pick-up unit is the form of a stroke or a combination of strokes.
The present invention also provides a kind of immunochromatography detection method, comprises the steps:
(1) a kind of immuno-chromatography detection device is provided, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of the light path cavity of surveyed area and control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector are connected with microcontroller respectively with power circuit, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area;
(2) contacted with the test strips injection port of shell end by liquid sample, liquid sample flow in test strips, start detection device;
(3) be placed in the surveyed area in the light source irradiation test strips in surveyed area light path cavity, the photodetector be placed in surveyed area light path cavity detects the light reflected from surveyed area, is converted into electric signal, is sent to microcontroller plate; Be placed in the control area in the light source irradiation test strips in the light path cavity of control area, the photodetector be placed in the light path cavity of control area detects the light reflected from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects;
(4) microcontroller received current signal, carries out conversion process analysis, Treatment Analysis result is shown by LCDs.
beneficial effect
Compared with prior art, light transmission immuno-chromatographic test paper strip is set in pick-up unit of the present invention, utilize the perspectivity of reaction marking thing on nitrocellulose filter, can above test strips and below respectively surveyed area and control area are detected, the light path cavity of surveyed area and the separate setting of light path cavity of control area, completely cut off the mutual interference between surveyed area and control area detection light completely, enhance the reliability of testing result.Test strips and pick-up unit are wholely set, and automatic start detection device at once after test strips pipette samples, testing result can be shown to user by LCDs after several minutes, operation is very easy, and various user uses unrestricted, meets family and use.Product miniaturization of the present invention, cost is low, is easy to produce, and simple to operate, and display result quantizes and directly perceived.
Accompanying drawing explanation
Fig. 1 is the structural drawing of device described in the embodiment of the present invention 1;
Fig. 2 is the structural drawing of device using state described in the embodiment of the present invention 1;
Fig. 3 is the cut-open view of device described in the embodiment of the present invention 1;
Fig. 4 is the principle of work block scheme of device described in the embodiment of the present invention 1.
Embodiment
For making to have a better understanding and awareness architectural feature of the present invention and effect of reaching, coordinating detailed description in order to preferred embodiment and accompanying drawing, being described as follows:
Embodiment 1
As shown in Figure 1, Figure 2, Figure 3 shows, immuno-chromatography detection device, comprise housing 2, the lid 1 be removably connected with housing, the LCDs 3 being located at surface of shell, the test strips 5 being located at enclosure interior and circuit of measurement and control, the end of housing is provided with the water suction rod 4 for sample introduction, one end of described water suction rod contacts with the test strips in housing, the other end of described water suction rod extends housing from enclosure interior to shell end, is placed in the chamber that lid and shell end are formed.In use, lid is taken off from housing, the water suction of shell end rod is immersed in liquid sample, liquid sample is flow in test strips along water suction rod.
Described test strips 5 is immuno-chromatographic test paper strips of light transmission, utilizes the perspectivity of reaction marking thing on nitrocellulose filter.Described test strips 5 has surveyed area 14 and control area 15, surveyed area light path cavity 6 is provided with above or below surveyed area, control area light path cavity 7 is provided with above or below control area, and surveyed area light path cavity 6 and control area light path cavity 7 are arranged on the not ipsilateral of test strips 5, specifically, if control area light path cavity 7 is arranged on the top of control area, then surveyed area light path cavity 6 is arranged on the below of surveyed area, if or control area light path cavity 7 is arranged on the below of control area, then surveyed area light path cavity 6 is arranged on the top of surveyed area.Surveyed area light path cavity 6 and control area light path cavity 7 li arrange separate light source and photodetector respectively, described surveyed area light path cavity is provided with the detection line detection window 8 with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window 9 with the control area intercommunication light path of test strips, such setting, can avoid the mutual interference between surveyed area and control area detection light completely when detecting.
As shown in Figure 4, described circuit of measurement and control comprises microcontroller 16, surveyed area light source 10, surveyed area photodetector 11, control area light source 12, control area photodetector 13, light path part, power circuit, testing circuit 17, described LCDs 3, light path part, surveyed area light source 10, surveyed area photodetector 11, testing circuit 17 is connected with microcontroller respectively with power circuit, described control area light source 12 is connected with microcontroller 16 by testing circuit 17 with control area photodetector 13, described surveyed area light source 10 and surveyed area photodetector 11 are located in surveyed area light path cavity 6, described control area light source 12 and control area photodetector 13 are located in control area light path cavity 7.
As shown in Figure 3, after liquid sample flows to test strips along water suction rod (in figure the direction of arrow), by the sample photodetector arranged in housing, after detecting the sample in test strips, send a signal to microcontroller, thus start detection device.
Surveyed area in light source irradiation test strips in microprocessor controls surveyed area light path cavity, the photodetector be placed in surveyed area light path cavity detects the light reflected from surveyed area, is converted into electric signal, is sent to microcontroller plate; Be placed in the control area in the light source irradiation test strips in the light path cavity of control area, the photodetector be placed in the light path cavity of control area detects the light reflected from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects.Microcontroller received current signal, carries out conversion process analysis, Treatment Analysis result is shown by LCDs.Treatment Analysis result can be quantitative analysis results or the qualitative analysis.
Embodiment 2
The present embodiment provides a kind of immunochromatography detection method, and the device that the present embodiment uses is identical with embodiment 1.
A kind of immunochromatography detection method, comprises the steps:
1, the device of a kind of enforcement described in 1 be provided.
2, contacted with the test strips injection port (absorb water rod) of shell end by liquid sample, liquid sample flow in test strips, automatic start detection device.
3, be placed in the surveyed area in the light source irradiation test strips in surveyed area light path cavity, the photodetector be placed in surveyed area light path cavity detects the light reflected from surveyed area, is converted into electric signal, is sent to microcontroller plate; Be placed in the control area in the light source irradiation test strips in the light path cavity of control area, the photodetector be placed in the light path cavity of control area detects the light reflected from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects.
4, microcontroller received current signal, carries out conversion process analysis, Treatment Analysis result is shown by LCDs.
More than show and describe the advantage of the ultimate principle of invention, principal character and invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; the just principle of the present invention described in above-described embodiment and instructions; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in claimed scope of the present invention.The protection domain of application claims is defined by appending claims and equivalent thereof.
Claims (9)
1. an immuno-chromatography detection device, it is characterized in that, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the control line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of the light path cavity of surveyed area and control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector are connected with microcontroller respectively with power circuit, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area.
2. immuno-chromatography detection device according to claim 1, it is characterized in that, described control area light path cavity is arranged on the top of control area, described surveyed area light path cavity is arranged on the below of surveyed area, or described control area light path cavity is arranged on the below of control area, and described surveyed area light path cavity is arranged on the top of surveyed area.
3. immuno-chromatography detection device according to claim 1, it is characterized in that, described circuit of measurement and control also comprises the testing circuit for detecting sample validity, and described control area light source is connected with microcontroller respectively by described testing circuit with control area photodetector.
4. immuno-chromatography detection device according to claim 1, is characterized in that, described test strips injection port is provided with the suction lining bar be communicated with test strips.
5. immuno-chromatography detection device according to claim 1, is characterized in that, described display is LCDs.
6. immuno-chromatography detection device according to claim 1, is characterized in that, is provided with the sample photodetector for automatically controlling testing circuit in described housing.
7. immuno-chromatography detection device according to claim 1, is characterized in that, described pick-up unit also comprises lid, and described lid and shell end are removably connected to form airtight cavity, and test strips injection port is sealed in above-mentioned cavity.
8. immuno-chromatography detection device according to claim 1, is characterized in that, the profile of described pick-up unit is the form of a stroke or a combination of strokes.
9. an immunochromatography detection method, is characterized in that, comprises the steps:
(1) a kind of immuno-chromatography detection device is provided, comprise housing, be located at the display of surface of shell, be located at the test strips with surveyed area and control area in housing, surveyed area light path cavity, control area light path cavity, circuit of measurement and control, be located at the test strips injection port of shell end, described surveyed area light path cavity is provided with the detection line detection window with ELISA test strip region intercommunication light path, described control area light path cavity is provided with the detection line detection window with the control area intercommunication light path of test strips, described test strips is light transmission immuno-chromatographic test paper strip, described surveyed area light path cavity and control area light path cavity are located at the not ipsilateral of test strips, make the light path cavity of the light path cavity of surveyed area and control area separate,
Described circuit of measurement and control comprises microcontroller, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector, light path part, power circuit, described display, light path part, surveyed area light source, surveyed area photodetector, control area light source, control area photodetector are connected with microcontroller respectively with power circuit, described surveyed area light source and surveyed area photodetector are located in surveyed area light path cavity, and described control area light source and control area photodetector are located in the light path cavity of control area;
(2) contacted with the test strips injection port of shell end by liquid sample, liquid sample flow in test strips, start detection device;
(3) be placed in the surveyed area in the light source irradiation test strips in surveyed area light path cavity, the photodetector be placed in surveyed area light path cavity detects the light reflected from surveyed area, is converted into electric signal, is sent to microcontroller plate; Be placed in the control area in the light source irradiation test strips in the light path cavity of control area, the photodetector be placed in the light path cavity of control area detects the light reflected from control area, be converted into electric signal, be resent to microcontroller, the validity that judgement sample detects;
(4) microcontroller received current signal, carries out conversion process analysis, Treatment Analysis result is shown by LCDs.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410040407.7A CN103760339B (en) | 2014-01-28 | 2014-01-28 | Immuno-chromatography detection device and method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410040407.7A CN103760339B (en) | 2014-01-28 | 2014-01-28 | Immuno-chromatography detection device and method |
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| CN103760339A CN103760339A (en) | 2014-04-30 |
| CN103760339B true CN103760339B (en) | 2016-01-13 |
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| CN109239338B (en) * | 2018-10-09 | 2023-09-08 | 邱华遗 | Wide-range immunochromatography detection device and method |
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| CN1573315A (en) * | 2003-06-04 | 2005-02-02 | 因韦尔尼斯医药瑞士股份有限公司 | Flow detection for judging assay results |
| CN201210144Y (en) * | 2008-03-28 | 2009-03-18 | 艾博生物医药(杭州)有限公司 | Optical analysis reading device |
| CN102323215A (en) * | 2011-08-05 | 2012-01-18 | 广州万孚生物技术有限公司 | Analyzing and reading device and method |
| CN103115897A (en) * | 2011-11-17 | 2013-05-22 | 艾博生物医药(杭州)有限公司 | Device for reading test results on test carrier |
| CN203759019U (en) * | 2014-01-28 | 2014-08-06 | 上海道原生物科技有限公司 | Immunochromatographic detection device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8128871B2 (en) * | 2005-04-22 | 2012-03-06 | Alverix, Inc. | Lateral flow assay systems and methods |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1573315A (en) * | 2003-06-04 | 2005-02-02 | 因韦尔尼斯医药瑞士股份有限公司 | Flow detection for judging assay results |
| CN201210144Y (en) * | 2008-03-28 | 2009-03-18 | 艾博生物医药(杭州)有限公司 | Optical analysis reading device |
| CN102323215A (en) * | 2011-08-05 | 2012-01-18 | 广州万孚生物技术有限公司 | Analyzing and reading device and method |
| CN103115897A (en) * | 2011-11-17 | 2013-05-22 | 艾博生物医药(杭州)有限公司 | Device for reading test results on test carrier |
| CN203759019U (en) * | 2014-01-28 | 2014-08-06 | 上海道原生物科技有限公司 | Immunochromatographic detection device |
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