CN1560024A - Functional fatty acid-amino acid complex and preparation method thereof - Google Patents

Abstract

The invention is a functional fatty acid-amino acid compound. The appearance of the complex is white or light yellow solid powder, in which the functional fatty acid content is 9.99-65.00%, the amino acid content is 34.99-90.00%, and the water content is 0.01-1.00%. The complex is hydrophilic, and its low concentration The aqueous solution is in a transparent state and has physiological activity, and the functional fatty acid in the emulsion state is precipitated in the solution under acidic conditions. The functional fatty acids are: conjugated linoleic acid (CLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (AA), gamma-linolenic acid (GLA) . The amino acids are: lysine, arginine. The complex has the characteristics of anti-oxidation, easy storage and wide application range, and is easy to be absorbed and utilized by organisms. The preparation method of the complex has simple steps, easy operation, and the yield is as high as 90-97%.

Description

Functional fatty acid-amino acid complex and preparation method thereof

technical field

The invention relates to the improvement of biochemical raw materials used in the technical fields of food, functional food, medicine and its auxiliary materials, food additives, feed additives, etc., specifically a functional fatty acid-amino acid compound, which belongs to the technical field of biochemical industry.

Background technique

In the past decade or so, the research on active lipids has been an active field in the world. Some active substances naturally occurring in humans and animals, such as: eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (AA), gamma-linolenic acid (GLA) And other functional fatty acids, because they do not have any toxicity, the research on their physiological effects and functions has gradually attracted people's attention. Conjugated linoleic acid (CLA) is also a kind of functional fatty acid of linoleic acid. These functional fatty acids have physiological functions such as anti-arteriosclerosis, anti-oxidation ^[1-2] , improving immunity, increasing bone density, reducing body fat in humans and animals, and increasing muscle ^[3-5] . Existing functional fatty acid products, such as conjugated linoleic acid (CLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (AA), γ- Linolenic acid (GLA) is mostly hydrophobic oil, which is applied in the form of capsules and microcapsules. As an additive type, it is usually added in the form of microcapsules, which are difficult to add to the liquid base material, and are easy to separate after emulsification.

As we all know, lysine (L-Lysine) is an essential amino acid, which cannot be synthesized by the human body. Lysine (Lysine) plays an important role in human protein synthesis and growth and development. It can improve human digestion and absorption capacity, accelerate protein synthesis, and provide basic materials for growth and development. Arginine (L-Arginine) is a conditional essential amino acid that can regulate the secretion of various endogenous hormones in the human body, such as the growth hormone secreted by the pituitary gland, which can promote the division and proliferation of cartilage cells and accelerate bone growth; regulate brain cell blood Flow, enhance the long-term memory function of the brain. Arginine is very important in muscle metabolism, which can promote muscle gain and fat loss, so it will not cause obesity. Arginine has an improving effect on blood sugar tolerance of type 2 diabetes.

Contents of the invention

The object of the present invention is to provide a functional fatty acid-amino acid complex and a preparation method thereof in order to expand the application range of functional fatty acids and essential amino acids. The prepared compound should be a solid powder with hydrophilic characteristics.

The purpose of the present invention is achieved by the following technical solutions, and a functional fatty acid-amino acid complex has been developed. The appearance of the complex is white or light yellow solid powder, in which the functional fatty acid content is 9.99-65.00%, the amino acid content is 34.99-90.00%, and the water content is 0.01-1.00%; the complex is hydrophilic and low The concentrated aqueous solution is in a transparent state, and has the physiological activity of functional fatty acids and amino acids. Under acidic conditions, the functional fatty acids in the state of emulsion are separated out.

The functional fatty acids include: conjugated linoleic acid (CLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (AA), gamma-linseed One or more of these acids (GLA).

The amino acids include: lysine, arginine, single or mixed amino acids.

The low-concentration aqueous solution of the functional fatty acid-amino acid complex has a concentration of 0.01-2.00%, and the acidic condition of the solution is pH 1-3.

The steps of the preparation method of the functional fatty acid-amino acid complex are as follows:

(1), adding the amino acid weighed by feeding intake to 2-6 times the weight of clean water to dissolve, to obtain an amino acid solution;

(2) Add functional fatty acids into the amino acid solution according to the feeding ratio, and stir and react for 10-30 minutes at 30-50° C. to obtain a white viscous semi-solid product.

(3), the product obtained in step (2) is distilled under reduced pressure, and the moisture is removed to obtain a white or light yellow solid powdery substance, which is ground and sieved to obtain a granular hydrophilic functional fatty acid with a particle size of 60-80 mesh -Amino acid complex.

Described (three) step, also can add the ethanol of its total volume 10% in (two) step gained product, eliminate foam, gained product solution is through spray drying again, and its process condition of spray drying: inlet temperature 120-200 ℃, the outlet temperature is 60-90℃, the air intake is 24-400m ³ /h, and the spray air pressure is 1.0-1.5kg/cm ³ , and the appearance is white or light yellow solid granular hydrophilic functional fatty acid-amino acid complex product.

The advantage of the present invention is that because the appearance of the complex is white or light yellow solid powder, the functional fatty acid content is 9.99-65.00%, the amino acid content is 34.99-90.00%, and the water content is only 0.01-1.00%; Contains a high proportion of effective active substances. Moreover, the complex is hydrophilic, and the aqueous solution containing 2% of the complex is in a transparent state. Under acidic conditions, fatty acids are precipitated, which has the characteristics of anti-oxidation, which makes the complex easy to store and has a wide range of applications. The complex retains the physiological functions of raw fatty acids and amino acids, and is easily absorbed and utilized by organisms. The preparation method of the functional fatty acid-amino acid complex of the invention has simple steps, easy operation, and a yield as high as 90-97%.

Example

The embodiments of the present invention embody the protection scope of this patent, but the protection scope of this patent is not limited to the following embodiments.

Example 1: Take 1.0 g of lysine, add 6.0 ml of distilled water to dissolve; weigh 2.0 g of CLA, add it dropwise to the lysine solution at room temperature, stir and react for 30 minutes, the product is foamy, evaporate to remove water, and obtain a solid complex. After grinding and sieving, the granular conjugated linoleic acid lysine complex is obtained. The mass of the complex is 2.82 g, and the yield is 94.2%.

Example 2: Take 1.0 g of arginine, add 6.0 milliliters of distilled water to dissolve, weigh 2.0 grams of fish oil fatty acid (containing 11.2% of eicosapentaenoic acid (EPA) and 18.5% of docosahexaenoic acid (DHA), It was added dropwise into the lysine solution at room temperature, stirred for 20 minutes, and evaporated to remove water to obtain a solid complex. After grinding and sieving, the granular fish oil fatty acid amino acid complex is obtained, the mass of the complex is 2.71 g, and the yield is 90.8%.

Example 3: Weigh 0.5 g of arginine and 0.5 g of lysine, dissolve them in 6.0 ml of distilled water, weigh 2.0 g of CLA, add dropwise to the amino acid solution at 50° C., and stir for 10-30 minutes. Add 10% ethanol by volume to the product to eliminate foam. The product solution is spray-dried, and the spray-drying process conditions are as follows: inlet temperature 120-200°C, outlet temperature 60-90°C, air intake volume 24-400m ³ /h, spray air pressure 1.0-1.5kg/cm ³ ; to obtain particles 2.91 grams of hydrophilic fatty acid complex, yield 97.0%.

Example 4: Take 0.1 g of the conjugated linoleic acid-lysine complex prepared in Example 1, add 5.0 g of distilled water to dissolve, and the solution is clear and transparent. Experiments show that the complex has hydrophilic and emulsifying activity. It is dissolved in water at a low concentration (0.5%), and micelles appear at a concentration greater than this (5%). The aqueous solution of conjugated linoleic acid lysine complex was heated at 100° C. for 5 minutes, and the aqueous solution did not change significantly. Experiments show that the aqueous solution of the complex is stable when heated. Add appropriate amount of 1M hydrochloric acid to the aqueous solution of conjugated linoleic acid lysine complex, the complex aqueous solution immediately becomes turbid, and an oil layer floats out. Experiments show that the complex is unstable in case of acid and easily decomposed.

Those skilled in the art will understand that within the protection scope of the present invention, modifications, additions and substitutions are all possible to the above embodiments, but none of them exceed the protection scope of the present invention.

Claims (6)

1. A functional fatty acid-amino acid complex, characterized in that: the complex is white or light yellow solid powder in appearance, wherein the functional fatty acid content is 9.99-65.00%, the amino acid content is 34.99-90.00%, and the moisture content is 0.01- 1.00%; the complex is hydrophilic, and the low-concentration aqueous solution of the complex is in a transparent state, and has the physiological activity of functional fatty acids and amino acids, and the solution has the precipitation of functional fatty acids in an emulsion state under acidic conditions. 2. The functional fatty acid-amino acid complex according to claim 1, characterized in that: the functional fatty acid comprises: conjugated linoleic acid (CLA), eicosapentaenoic acid (EPA), eicosapentaenoic acid (EPA), Carbasahexaenoic acid (DHA), arachidonic acid (AA), γ-linolenic acid (GLA), one or more of them. 3. The functional fatty acid-amino acid complex according to claim 1, characterized in that: said amino acids include: lysine, arginine, single or mixed amino acids. 4. The functional fatty acid-amino acid complex according to claim 1, 2 or 3, characterized in that: the low-concentration aqueous solution of the functional fatty acid-amino acid complex has a concentration of 0.01--2.00%, and the acidity of the solution is Conditions were pH 1-3. 5. The method for preparing the functional fatty acid-amino acid complex according to claim 1, 2 or 3, characterized in that the steps of the method are as follows: (1), adding the amino acid weighed by feeding intake to 2-6 times the weight of clean water to dissolve, to obtain an amino acid solution; (2) Add functional fatty acids into the amino acid solution according to the feeding ratio, and stir and react for 10-30 minutes at 30-50° C. to obtain a white viscous semi-solid product. (3), the product obtained in step (2) is distilled under reduced pressure, and the moisture is removed to obtain a white or light yellow solid powdery substance, which is ground and sieved to obtain a granular hydrophilic functional fatty acid with a particle size of 60-80 mesh -Amino acid complex. 6, according to the preparation method of the described functional fatty acid-amino acid compound of claim 5, it is characterized in that: described (three) step, also can add its total volume 10% ethanol in (two) step gained product, eliminate Foam, the resulting product solution is then spray-dried, and the spray-drying process conditions: inlet temperature 120-200°C, outlet temperature 60-90°C, air intake 24-400m ³ /h, spray air pressure 1.0-1.5kg/cm ^3. Obtain a white or light yellow solid granular hydrophilic functional fatty acid-amino acid complex product.