CN1315389C - Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract - Google Patents
Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract Download PDFInfo
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- CN1315389C CN1315389C CNB2005100273349A CN200510027334A CN1315389C CN 1315389 C CN1315389 C CN 1315389C CN B2005100273349 A CNB2005100273349 A CN B2005100273349A CN 200510027334 A CN200510027334 A CN 200510027334A CN 1315389 C CN1315389 C CN 1315389C
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Abstract
一种用合欢提取物制备控制黄瓜和番茄病害水剂的方法，用于农药领域。本发明采集合欢鲜叶，整理晾干后干燥粉碎成粉末；用10-20倍于合欢叶粉末重量的无水甲醇作为提取溶剂；在室温条件下浸渍提取，将粗提液用减压浓缩的方法，将其浓缩至膏状，得合欢叶浸膏；合欢叶浸膏中加入相当于合欢叶质量2.5倍体积的蒸馏水，然后置于超声波发生器中将干物质悬浮，再依次用等体积的石油醚、乙酸乙酯、水饱和正丁醇各萃取5次，正丁醇萃取液减压浓缩至干，即得合欢叶提取物，用水溶解得水剂。本发明所制备的水剂对环境无污染，对人、畜安全；原料丰富；提取工艺简单；对黄瓜霜霉病和番茄灰霉病有很好的预防效果。The invention discloses a method for preparing an aqueous agent for controlling cucumber and tomato diseases by using the extract of Albizia Julibrissin, which is used in the field of pesticides. The present invention collects the fresh leaves of Albizia Julibrissin, drys and pulverizes them into powder; uses anhydrous methanol 10-20 times the weight of Albizia Julibrissin leaf powder as the extraction solvent; soaks and extracts at room temperature, and concentrates the crude extract with decompressed The method is to concentrate it into a paste to obtain the leaf extract of Albizia Julibrissin; add distilled water equivalent to 2.5 times the volume of leaves of Albizia Julibrissin to the leaf extract of Albizia Julibrissin, then place it in an ultrasonic generator to suspend the dry matter, and then use an equal volume of Petroleum ether, ethyl acetate, and water-saturated n-butanol were extracted 5 times each, and the n-butanol extract was concentrated to dryness under reduced pressure to obtain the Albizia Julibrissin leaf extract, which was dissolved in water to obtain an aqueous solution. The water preparation prepared by the invention has no pollution to the environment and is safe to humans and animals; the raw material is rich; the extraction process is simple; and the invention has a good preventive effect on cucumber downy mildew and tomato gray mold.
Description
Technical field
That the present invention relates to is a kind of preparation method of technical field of pesticide, is a kind of method for preparing control cucumber and tomato disease aqua with albizzia julibrissin extract.
Background technology
Cucumber and tomato are the main vegetable varieties that liked by citizen, and cucumber downy mildew and graw mold of tomato are to perplex the especially important fungal disease of facility cultivation of vegetable growing in recent years, but general underproduction 10%-20%, and the underproduction is more than 50% when serious.Generally use chemical pesticide and control such disease at present, and chemical pesticide is to environment, harm that human health caused, and become common recognition by problems such as controlling object easily develop immunity to drugs.The goal of the invention of this patent promptly utilizes the aqua of Nantural non-toxic plant albizzia julibrissin extract preparation to control the generation of these two kinds of diseases.Be to reduce the use of chemical pesticide, produce green or organic vegetable provides technical basis.Silk tree (Albizzia julibrissin Ddrazz) belongs to leguminous plant, and have at China's middle part wide geographic area more and plant, aboundresources, and also it still is a kind of traditional Chinese medicine of can calm the nerves Xie Yu, activating blood circulation and reducing swelling.
Through the prior art literature search is found, Zhao Boguang is at " Nanjing Forestry University's journal " (the 18th volume, the 2nd phase) is entitled as: reported that the silk tree bark extract has the food refusal effect to dendrolimus punctatus larvae in one piece of article of " six plant species extracts press down food effect and toxicity to dendrolimus punctatus ", this extract is that the bark with silk tree is a raw material, use Soxhlet extractor, earlier after benzinum (30～60 ℃) extracts degreasing, analyze the straight alcohol extraction with 95% again and obtain ethanol extract, but the leaf extract that does not have silk tree carries out any research; In further retrieving, there is not to find to be used for the research report of controlling plant diseases about the silk tree leaf.
Summary of the invention
The objective of the invention is at above shortcomings in the prior art, a kind of method for preparing control cucumber and tomato disease aqua with albizzia julibrissin extract is provided, make it have higher preventive effect to cucumber downy mildew and graw mold of tomato, can be directly used in these two kinds of diseases of control, and have nuisanceless, pollution-free and do not have the characteristics of residual hazard, avoided the harm of chemical pesticide.
The present invention is achieved by the following technical solutions, and each weight percentages of components of described aqua is: silk tree leaf extract 0.95-3%, and surplus is a water, method step is as follows:
(1) gather the bright leaf of silk tree, arrangement is dried the back and be dried and crushed into 20-40 order powder under 30-35 ℃;
(2) with 10-20 doubly to the absolute methanol of silk tree leaf powder weight or absolute ethyl alcohol or 95% ethanol as extracting solvent;
(3) dipping extracts 3 times at ambient temperature, each 24 hours; With the method for crude extract with concentrating under reduced pressure, it is concentrated into paste, get silk tree leaf medicinal extract;
(4) add the distilled water that is equivalent to 2.5 times of volumes of silk tree leaf quality in the silk tree leaf medicinal extract, place supersonic generator that dry matter is suspended then; Respectively extract 5 times with isopyknic benzinum, ethyl acetate, water-saturated n-butanol successively again; Butanol extraction liquid is evaporated to dried, promptly gets the silk tree leaf extract, water dissolve aqua.Water is distilled water or deionized water.
The present invention compared with prior art has following advantage: this biocide preparation derives from the natural traditional Chinese medicine plant extracts, and utilizes its water-soluble characteristics, is mixed with aqua, so environmentally safe; To people, animal safety, be a kind of pollution-free plant source biological bactericide; This sterilization aqua is that wide to distribute, resourceful silk tree is a raw material, and is easy to artificial cultivation; Extraction process is simple, need not special instrument, and the solvent after the recovery also can reuse; Said preparation all has preventive effect preferably to cucumber downy mildew and graw mold of tomato.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment:
Embodiment 1:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 0,95%, distilled water 99.05%.
Take by weighing silk tree leaf powder (20 order particle) 20g, extract 3 times with 95% ethanol 200ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 3.1g; Utilize ultrasonic, with 50ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, n-butyl alcohol phase extract concentrates, obtain extract 0.95g, filter, be mixed with concentration and be 0.95% aqua with the water-soluble extract of separating of 100ml; Adopt the leaf disc floating method that cucumber downy mildew is carried out indoor prophylactic tria, the preventive effect of cucumber downy mildew is reached 88.89%.
Embodiment 2:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 3%, distilled water 97%.
Take by weighing silk tree leaf powder (20 order particle) 40g, extract 3 times with absolute methanol 400ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 10.3g; Utilize ultrasonic, with 100ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, after concentrating, n-butyl alcohol phase extract gets extract 1.9g, with the water-soluble extract of separating of 63.33ml, filter, be mixed with concentration and be 3% aqua; Adopt indoor pot experiment that cucumber downy mildew is carried out prophylactic tria, its preventive effect reaches 62.00%.
Embodiment 3:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 0.95%, water 99.05%.
Take by weighing silk tree leaf powder (20 order particle) 40g, extract 3 times with absolute methanol 400ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 10.3g; Utilize ultrasonic, with 100ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, n-butyl alcohol phase extract obtains extract 1.9g after concentrating, get thing with the water-soluble Xie Di of 200ml, filter, be mixed with concentration and be 0.95% aqua; Adopt field plot trial that cucumber downy mildew is carried out prophylactic tria, its preventive effect reaches 48.84%.This control efficiency and blank (spraying water) relatively are remarkable difference.
Embodiment 4:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 1.2%, distilled water 98.8%.
Take by weighing silk tree leaf powder (20 order particle) 20g, extract 3 times with absolute methanol 200ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and obtains dry matter 4.9g; Utilize ultrasonic, with 50ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, n-butyl alcohol phase extract obtains extract 1.2g after concentrating, with the water-soluble extract of separating of 60.6ml, filter, be mixed with concentration and be 1.98% aqua.Adopt the influence of inhibition zone method mensuration to the graw mold of tomato bacteria growing, its inhibiting rate is 100%; Adopt the influence of sessile drop method mensuration to the graw mold of tomato spore germination, its inhibiting rate is 86.30%.
Embodiment 5:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 3%, distilled water 97%.
Take by weighing silk tree leaf powder (20 order particle) 40g, extract 3 times with absolute methanol 400ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 10.3g; Utilize ultrasonic, with 100ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, n-butyl alcohol phase extract obtains extract 1.9g after concentrating, with the water-soluble extract of separating of 63.33ml, filter, be mixed with concentration and be 3% aqua.The fruit method that exsomatizes is measured and is shown that this aqua is 70.12% to the control rate of graw mold of tomato.
Embodiment 6:
Control cucumber and tomato disease aqua percentage by weight: silk tree leaf extract 0.95%, distilled water 99.05%.
Take by weighing silk tree leaf powder (20 order particle) 40g, extract 3 times with absolute methanol 400ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 10.3g; Utilize ultrasonic, with 100ml water dry matter is suspended, equal-volume benzinum, ethyl acetate, water-saturated n-butanol extract 5 times successively, n-butyl alcohol phase extract obtains extract 1.9g after concentrating, with the water-soluble extract of separating of 200ml, filter, be mixed with concentration and be 0.95% aqua; Adopt field plot trial to measure the harm situation of graw mold of tomato to titbit and young fruit, its preventive effect reaches 81.02%.
Claims (4)
1. one kind prepares the method for control cucumber and tomato disease aqua with albizzia julibrissin extract, it is characterized in that, comprises that step is as follows:
(1) gather the bright leaf of silk tree, back drying and crushing powdered is dried in arrangement;
(2) with 10-20 doubly to the absolute methanol of silk tree leaf powder weight as extracting solvent;
(3) dipping extracts at ambient temperature, with the method for crude extract with concentrating under reduced pressure, it is concentrated into paste, gets silk tree leaf medicinal extract:
(4) add the distilled water that is equivalent to 2.5 times of silk tree leaf powder qualities in the silk tree leaf powder medicinal extract, place supersonic generator that dry matter is suspended then, respectively extract 5 times with isopyknic benzinum, ethyl acetate, water-saturated n-butanol successively again, butanol extraction liquid is evaporated to dried, promptly get the silk tree leaf extract, water dissolve aqua.
2, require describedly to prepare the method for control cucumber and tomato disease aqua according to right 1, it is characterized in that in the step (1), arrangement is dried the back and be dried and crushed into 20-40 order powder under 30-35 ℃ with albizzia julibrissin extract.
3, require describedly to prepare the method for control cucumber and tomato disease aqua according to right 1, it is characterized in that in the step (3), room temperature condition dipping down extracts each 24 hours 3 times with albizzia julibrissin extract.
4, require describedly to prepare the method for control cucumber and tomato disease aqua according to right 1, it is characterized in that in the step (4), water is distilled water or deionized water with albizzia julibrissin extract.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100273349A CN1315389C (en) | 2005-06-30 | 2005-06-30 | Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract |
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| CNB2005100273349A CN1315389C (en) | 2005-06-30 | 2005-06-30 | Method for preparing solution for controlling cucumber and tomato disease using albizzia julibrissin extract |
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| CN1709067A CN1709067A (en) | 2005-12-21 |
| CN1315389C true CN1315389C (en) | 2007-05-16 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461386B (en) * | 2008-12-30 | 2012-10-24 | 淮阴师范学院 | Bastard tamarind legume extract, and preparation method thereof and anti-bacteria agent |
| CN101518267B (en) * | 2009-04-02 | 2013-06-19 | 上海交通大学 | Aqueous pesticide solution compounded by silver chain extract and tea saponin and method for preparing same |
| CN101564052B (en) * | 2009-05-21 | 2012-09-19 | 上海交通大学 | Preparation method of water agent for preventing and controlling rape sclerotinia |
| CN102224829A (en) * | 2011-04-26 | 2011-10-26 | 上海市浦东新区农业技术推广中心 | Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases |
| CN106234429A (en) * | 2016-07-28 | 2016-12-21 | 上海交通大学 | One contains gallic compound plant bactericide and preparation thereof and application |
| CN112661575A (en) * | 2020-12-31 | 2021-04-16 | 广西易多收生物科技有限公司 | Fulvic acid type water-soluble bacterial fertilizer and preparation method thereof |
-
2005
- 2005-06-30 CN CNB2005100273349A patent/CN1315389C/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 中草药制剂防治黄瓜霜霉病研究 丁兆龙 时呈奎 岳凤荣 迟玉成 赵胜文 万继兴，山东农业大学学报，第26卷第03期 1995 * |
| 六种植物提取物对马尾松毛虫的抑食作用及毒性 赵博光，南京林业大学学报，第18卷第02期 1998 * |
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Granted publication date: 20070516 Termination date: 20110630 |