CN1340360A - 蛋黄免疫活性蛋白复合物 - Google Patents
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Abstract
蛋黄免疫活性蛋白复合物,以其总重量计含抗肠毒素免疫球蛋白45~55%、卵转铁蛋白17~23%、溶菌酶17~23%和余量的水分、灰分及微量元素。其中抗肠毒素免疫球蛋白其分子量为172KD(1720000),为2条重链H和2条轻链L,成Y字形,其中轻链L分子量~23KD(230000),重链H分子量~63KD(630000)。本发明的产品食用后能补充人体营养素,并对肠道内致病菌有抑制、吞噬作用,能提高肌体防病抗病能力。
Description
本发明涉及蛋白质复合物。
鸡蛋营养价值高,食用后对补充人体营养素,增强体质有好处。但至今人们尚未从鸡蛋中提取出一种具有更高功效的物质,这种物质既能补充营养素,又能抑制、吞噬肠道内的致病性细菌。已知技术中也未曾有过本发明的这种复合物。
本发明的目的在于提供一种含有多种氨基酸和微量元素的、食用后能抑制、吞噬体内致病性细菌的结晶型蛋白复合物。
本发明的技术解决方案其特殊之处是含有如下组分:抗肠毒素免疫球蛋白、卵转铁蛋白和溶菌酶。
本发明的复合物以其总重量计含抗肠毒素免疫球蛋白45~55%、卵转铁蛋白17~23%、溶菌酶17~23%和余量的水分、灰分及微量元素。
所述的复合物以其总重量计,优选的组分含量为抗肠毒素免疫球蛋白50%、卵转铁蛋白20%、溶菌酶20%和余量的水分、灰分及微量元素。
本发明的复合物,所述的抗肠毒素免疫球蛋白其分子结构模式为:其分子量为172KD(1720000),为2条重链H和2条轻链L,成Y字形,其中轻链L分子量~23KD(230000),重链H分子量~63KD(630000)。
本发明的复合物经此方法制取:采用肠毒素类的细胞菌作抗原,制备成疫苗,致病性大肠杆菌和沙门氏菌之比为60∶40,对产蛋母鸡定期强化注射,经一段时间的喂养后,母鸡产蛋取之蛋黄进行稀释、絮凝、离心过滤、超滤、微滤、冷冻干燥而成。
所述的复合物为结晶体,呈浅粉红色,无异味。其中:
抗肠毒素免疫球蛋白的功效:能够凝集、吞噬及中和细菌和排除毒素成份,能杀死或抑制常见的肠道致病菌,如金黄色葡萄球菌、绿脓杆菌、链球菌、沙门氏菌;
卵转铁蛋白的功效:可促进淋巴细胞的生长,防止或抑制致病菌侵入,能调节和促进短双歧杆菌生长,能结合并输送铁离子,从而促进婴幼儿对铁的吸收,有效防止贫血;可抑制体内自由基的生成,具有缓解类风湿关节炎的抗衰老的作用;
溶菌酶的功效:有防止肠炎和变态反应作用,是双歧杆菌增长因子,对婴幼儿的肠道菌群有平衡作用;具有分解革兰氏阳性菌的作用;具有预防龋齿的功效,是一种安全性很高的食品用杀菌剂。
本发明的复合物,其总蛋白含量可高达90%,各种氨基酸含量由表1所示(100克本复合物所含氨基酸克数):
表1
| 氨基酸名称 | 含量(g/100g) |
| 天六冬氨酸 | 9.48 |
| 苏氨酸 | 4.36 |
| 丝氨酸 | 5.80 |
| 谷氨酸 | 12.21 |
| 甘氨酸 | 3.05 |
| 丙氨酸 | 5.26 |
| 胱氨酸 | 3.59 |
| 缬氨酸 | 6.00 |
| 蛋氨酸 | 3.26 |
| 异亮氨酸 | 3.77 |
| 亮氨酸 | 7.04 |
| 酪氨酸 | 3.52 |
| 苯丙氨酸 | 3.81 |
| 赖氨酸 | 6.88 |
| 组氨酸 | 2.47 |
| 精氨酸 | 4.42 |
| 色氨酸 | 0.89 |
| 脯氨酸 | 4.17 |
| 总氨基酸 | 89.98 |
本发明的复合物所含微量元素由表2所示(每千克复合物所含毫克数):
表2
| 成分 | 含量(mg/kg) |
| Co | 0.062 |
| Cd | 0.032 |
| Mg | 145 |
| Cu | 3.47 |
| Zn | 10.6 |
| Fe | 42.7 |
| Pb | 0.036 |
| Cr | 2.38 |
本发明的复合物,由于富含抗肠毒素免疫球蛋白,故具有抗肠毒素的抗体,又由于含卵转铁蛋白和溶菌酶,而具有明显的聚集抑菌和吞噬细菌的效果。本发的明复合物的主要作用和效果:能抑制人体肠道内的致病性菌株,且对其具有吞噬作用;具有治疗腹泻和肠道炎症的效果,有效地提高肌体的防病抗病能力;本发明的复合物无毒副作用,无激素,肌体对此产品无任何依赖性,是人体获得保护性被动免疫的活性蛋白。
本发的产品用法:婴儿直接冲调口服,儿童、成人在饭后或饱腹后服用,免用50℃以上热水冲调,避免与强酸、强碱混合。
Claims (5)
1、蛋黄免疫活性蛋白复合物,其特征是含有如下组分:抗肠毒素免疫球蛋白、卵转铁蛋白和溶菌酶。
2、如权利要求1所述的复合物,其特征是以该复合物总重量计含抗肠毒素免疫球蛋白45~55%、卵转铁蛋白17~23%、溶菌酶17~23%和余量的水分、灰分及微量元素。
3、如权利要求1所述的复合物,其特征是以该复合物总重量计含抗肠毒素免疫球蛋白50%、卵转铁蛋白20%、溶菌酶20%和余量的水分、灰分及微量元素。
4、如权利要求1、2或3所述的复合物,其特征是所述的抗肠毒素免疫球蛋白其分子量为172KD(1720000),为2条重链H和2条轻链L,成Y字形,其中轻链L分子量~23KD(230000),重链H分子量~63KD(630000)。
5、如权利要求4所述的复合物,其特征是该复合物经此方法制取:采用肠毒素类的细胞菌作抗原,制备成疫苗,致病性大肠杆菌和沙门氏菌之比为60∶40,对产蛋母鸡定期强化注射,经一段时间的喂养后,母鸡产蛋取之蛋黄进行稀释、絮凝、离心过滤、超滤、微滤、冷冻干燥而成。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101605453B (zh) * | 2007-02-15 | 2011-10-26 | 国立大学法人冈山大学 | 使用溶菌酶、其盐或生物学片段、或溶菌酶-相关肽作为卵识别信息素的害虫灭杀 |
| CN111803628A (zh) * | 2020-07-24 | 2020-10-23 | 尤丽康(江苏)生物医药有限公司 | 一种抗幽门螺杆菌复合制剂 |
-
2000
- 2000-08-31 CN CN 00125941 patent/CN1340360A/zh active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101605453B (zh) * | 2007-02-15 | 2011-10-26 | 国立大学法人冈山大学 | 使用溶菌酶、其盐或生物学片段、或溶菌酶-相关肽作为卵识别信息素的害虫灭杀 |
| CN111803628A (zh) * | 2020-07-24 | 2020-10-23 | 尤丽康(江苏)生物医药有限公司 | 一种抗幽门螺杆菌复合制剂 |
| CN111803628B (zh) * | 2020-07-24 | 2021-05-14 | 尤丽康(江苏)生物医药有限公司 | 一种抗幽门螺杆菌复合制剂 |
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