CN1209973C - Oil solution of insecticide Bacillus thuringiensis - Google Patents

Abstract

The invention belongs to the technical field of microbial insecticide and pesticide, and specifically relates to the technical field of preparation of bacillus thuringiensis insecticide oil. The oil agent is prepared by solvent oil, emulsifier and bacillus thuringiensis powder in a certain proportion. The solvent oil wherein is selected from vegetable oil and mineral oil, and emulsifier is selected from mineral oil or emulsifier. Compared with the prior art, the invention improves the stability of the bacillus thuringiensis insecticide and effectively prolongs the storage period of the bacillus thuringiensis insecticide, reduces production cost and reduces environmental pollution.

Description

A kind of Bacillus thuringiensis insecticide oil

The invention belongs to the technical field of manufacturing bacillus thuringiensis insecticide oil, and relates to the preparation of microbial pesticide oil.

Bacillus thuringiensis is currently the most widely used microbial insecticide in the world. At the same time, the application of Bacillus thuringiensis insecticides also exposed some problems that need to be solved urgently, such as slow insecticidal speed, short residual effect period, and susceptibility to environmental factors, etc., which are generally solved through the improvement of dosage forms. At present, the existing Bacillus thuringiensis oil agent product has Golden Bear (GB-111) (Floore-TG, Clement-BW Jr et al Journal of the Florida Mosquito Control Association 1991, 62: 241～44), GB-111 is a A mineral oil-based oil. This product has good adhesion, spreadability, and good ability to resist rain erosion. However, all mineral oils are used as solvent oils, which inevitably have a certain impact on the environment.

The purpose of the invention is to overcome the defects of the prior art, and to develop a Bacillus thuringiensis insecticide oil that has excellent performance and can reduce environmental pollution.

The present invention is realized through the following technical solutions:

A bacillus thuringiensis insecticide oil preparation is made up of solvent oil, emulsifier and former powder of bacillus thuringiensis, and its formula and manufacturing steps comprise:

1) The solvent oil used is selected from vegetable oil and mineral oil;

2) The emulsifier used is selected from diesel oil, gasoline, kerosene, paraffin oil, petroleum ether, agricultural milk 300, agricultural milk 100, agricultural milk 500, agricultural milk 602 and/or Tween 20;

3) The original powder used is concentrated dry powder of Bacillus thuringiensis;

The solvent oil is selected from rapeseed oil, white oil, diesel oil or palm oil.

The solvent oil is selected from rapeseed oil, white oil or diesel oil.

The emulsifier is selected from petroleum ether and agricultural milk 602.

The ratio of the oil agent is rapeseed oil: white oil: agricultural milk 602: petroleum ether is 5 ~ 9: 2 ~ 4: 2 ~ 4: 1 ~ 2. The best ratio is rapeseed oil: white oil: agricultural milk 602: Petroleum ether is 7: 3: 3: 1, and the original powder of Bacillus thuringiensis is prepared according to the mixture of every gram of the original powder and 1.4--4 milliliters of solvent oil and emulsifier. 2.8 ml of mineral spirits and a mixture of emulsifiers were prepared.

The proportioning ratio of the oil agent can also adopt another formula, and its proportioning is rapeseed oil: diesel oil: agricultural milk 602: petroleum ether is 5～9: 2～4: 2～4: 1～2. The best proportioning ratio For, rapeseed oil: diesel oil: agricultural milk 602: petroleum ether is 7:3:3:1, the original powder of Bacillus thuringiensis is prepared according to the mixture of each gram of original powder and 1.4--4 ml of solvent oil and emulsifier, the best The proportioning ratio is that every gram of raw powder is mixed with 2.8 milliliters of solvent oil core emulsifier.

The emulsifier in the above-mentioned oil formulation can also be formulated according to the following ratios: engine oil 0.2-0.8; gasoline 0.2-0.8; kerosene 0.2-0.8; paraffin oil 0.2-1.10; petroleum ether 0.5-1.0; , engine oil 0.3-0.6; gasoline 03-0.6; kerosene 0.4-0.6; paraffin oil 0.6-0.9; petroleum ether 0.6-0.8.

The preparation method of Bacillus thuringiensis insecticide oil preparation comprises: successively measure solvent oil, emulsifier, add in the container, after stirring evenly, then weigh and add the original powder of Bacillus thuringiensis, continue to stir until uniform, seal and store, namely become a finished product.

Implement the potency requirement of the original powder of Bacillus thuringiensis of the present invention to reach 32000.00IU/mg

Before the oil leaves the factory, its insecticidal toxicity is tested biologically according to conventional methods.

The present invention has the following positive effects

(1) Compared with the prior art, the present invention has less impact on environmental pollution.

(2) product quality of the present invention has bigger improvement, and in terms of toxicity, the decline rate of this oil agent toxicity potency is relatively small in the storage period, and under room temperature and constant temperature condition, decline rate is respectively when 12 months 20.4%, 22.9% and 18.2%.

(3) in the pot test, the oil agent residual effect period of the present invention is longer than the former powder, and the insecticidal effect is good in the residual effect period, and its result is shown in table 3:

Table 1 Comparison 1 of toxicity potency of oil preparations of the present invention with different storage periods time (month) condition repeat regression equation correlation coefficient Toxicity titer (IU/mg) rate of change 0 2 Y=1.539+3.471x 0.9661 26396 Y=3.934+1.544x 0.9143 26528 3 room temperature 2 Y=2.787+2.258x 0.9878 26487 -2% Y=3.838+1.386x 0.9837 25325 6 constant temperature 1 Y=4.065+1.133x 0.9400 26111 -1.3% room temperature 2 Y=3.639+1.198x 0.9624 20791 -18.7% Y=2.889+1.909x 0.9888 22250 constant temperature 1 Y=3.056+2.022x 0.9597 23233 -12.2% 9 room temperature 2 Y=4.011+1.077x 0.9305 20834 -20.1% Y=3.845+1.269x 0.9695 21478 constant temperature 1 Y=3.510+1.455x 0.9548 21514 -18.7% 12 room temperature 2 Y=3.814+1.232x 0.9776 21321 -20.4% Y=3.489+1.455x 0.9734 20806 constant temperature 1 Y=3.879+1.397x 0.9092 20393 -22.9%

Note: The tested insects are the third instar diamondback moth.

Table 2 Toxicity potency comparison two of oil preparations of the present invention in different storage periods time (month) condition repeat regression equation correlation coefficient Toxicity titer (IU/mg) rate of change 0 2 Y=3.519+1.479x 0.9794 31405 Y=3.080+1.586x 0.9847 28175 3 room temperature 2 Y=3.770+1.266x 0.9070 32882 +1.9% Y=3.870+1.600x 0.9614 27813 6 constant temperature 1 Y=3.524+1.719x 0.9999 24587 -17.5% room temperature 2 Y=4.017+1.550x 0.9557 34386 -18.7% Y=1.961+2.756x 0.9015 28343 constant temperature 1 Y=3.257+1.579x 0.9884 28831 -3.2% 9 room temperature 2 Y=3.428+1.377x 0.9802 27853 -17.1% Y=3.220+2.145x 0.9883 21572 constant temperature 1 Y=2.597+2.259x 0.9934 22665 -23.9% 12 room temperature 2 Y=3.594+1.392x 0.9959 26810 -18.2% Y=3.314+1.659x 0.9678 21907 constant temperature 1 Y=3.477+1.334x 0.9486 20521 -31.1%

Note: The tested insects are the third instar diamondback moth.

Table 3 The present invention and former powder are compared to the residual effect period of diamondback moth preparation Dilution factor Mortality rate on different days after application (%) 4 days 6 days 8 days 10 days 12 days 14 days Oil agent 2000 times 1 ^{note 1} 58.4 44.4 30.9 30.0 10.0 0.0 Oil agent 4000 times 1 ^{note 2} 48.6 32.1 21.6 13.3 6.7 0.0 Raw powder 5000 times 51.1 42.0 26.7 13.3 0.0 Raw powder 10000 times 44.9 28.2 18.1 10.0 0.0

Note: 1. The dilution factor is equivalent to 8000× dilution of the original powder in the oil agent;

2. The dilution factor is equivalent to 16000× dilution of the original powder in the oil agent;

3. The tested insect was the third instar diamondback moth.

Example 1:

Add rapeseed oil:white oil:Nongru 602:petroleum ether in a volume ratio of 9;4:4:2 per liter of oil. Then add 350g Bacillus thuringiensis powder

Example 2:

Add rapeseed oil:white oil:Nongru 602:petroleum ether in a volume ratio of 9;2:2:1 per liter of oil. Then add 350g Bacillus thuringiensis powder

Example 3:

Add rapeseed oil:white oil:Nongru 602:petroleum ether in a volume ratio of 5;4:4:2 per liter of oil. Then add 350g Bacillus thuringiensis powder

Example 4:

Add rapeseed oil: white oil: Nongru 602: petroleum ether in a volume ratio of 5; 2:2:1 per liter of oil. Then add 350g Bacillus thuringiensis powder

Example 5:

Add rapeseed oil:white oil:Nongru 602:petroleum ether in a volume ratio of 7;3:3:1 per liter of oil. Then add 350g Bacillus thuringiensis powder

Embodiment 6:

Add rapeseed oil: diesel oil: agricultural milk 602: petroleum ether in a volume ratio of 9; 4: 4: 2 per liter of oil. Then add 350g Bacillus thuringiensis powder

Embodiment 7:

According to the ratio of rapeseed oil in each liter of oil agent: diesel oil: agricultural milk 602: petroleum ether in a volume ratio of 9; 2:2:1, add. Then add 350g Bacillus thuringiensis powder

Embodiment 8:

Add rapeseed oil: diesel oil: agricultural milk 602: petroleum ether in a volume ratio of 5; 4: 4: 2 per liter of oil. Then add 350g Bacillus thuringiensis powder

Embodiment 9:

According to the ratio of rapeseed oil in each liter of oil agent: diesel oil: agricultural milk 602: petroleum ether in a volume ratio of 5; 2:2:1, add. Then add 350g Bacillus thuringiensis powder

Example 10:

Add rapeseed oil: diesel oil: agricultural milk 602: petroleum ether in a volume ratio of 7; 3:3:1 per liter of oil. Then add 350g Bacillus thuringiensis powder

Claims (12)

1, a bacillus thuringiensis insecticide oil preparation, is made up of solvent oil, emulsifier and former powder of bacillus thuringiensis, is characterized in that described formula and steps comprise: 1) The solvent oil used is selected from vegetable oil and mineral oil; 2) The emulsifier used is selected from diesel oil, gasoline, kerosene, paraffin oil, sherwood oil, concentrated milk 300, concentrated milk 100, concentrated milk 500, concentrated milk 602, and/or Tween 20; 3) The used raw powder is concentrated dry powder of Bacillus thuringiensis, which is prepared by mixing each gram of dry powder with 1.4-4 ml of solvent oil and emulsifier. 2. The Bacillus thuringiensis insecticide oil as claimed in claim 1, characterized in that said solvent oil is selected from rapeseed oil, white oil, diesel oil and/or palm oil. 3. The Bacillus thuringiensis insecticide oil as claimed in claim 1 or 2, characterized in that said solvent oil is selected from rapeseed oil, white oil and diesel oil. 4. The Bacillus thuringiensis insecticide oil as claimed in claim 1, characterized in that said emulsifier is selected from petroleum ether and Nongru 602. 5. The Bacillus thuringiensis insecticide oil as claimed in claim 1, characterized in that the ratio of the Bacillus thuringiensis dry powder to solvent oil and emulsifier mixture is: 1 gram of dry powder to 2.8 ml of solvent oil and Mixture of emulsifiers. 6. The Bacillus thuringiensis insecticide oil agent as claimed in claim 1, characterized in that the preparation ratio of the oil agent is rapeseed oil: white oil: agricultural milk 602: petroleum ether is 5-9: 2-4: 2 ~4:1~2. 7. The Bacillus thuringiensis insecticide oil agent as claimed in claim 1, characterized in that the preparation ratio of the oil agent is rapeseed oil: diesel oil: agricultural milk 602: petroleum ether is 5 ~ 9: 2 ~ 4: 2 ~ 4:1~2. 8. The Bacillus thuringiensis insecticide oil as claimed in claim 5, characterized in that the oil is prepared in the ratio of rapeseed oil: white oil: agricultural milk 602: petroleum ether = 7:3:3:1. 9. The Bacillus thuringiensis insecticide oil as claimed in claim 6, characterized in that the preparation ratio of the oil is rapeseed oil: diesel oil: agricultural milk 602: petroleum ether 7:3:3:1. 10. The Bacillus thuringiensis insecticide oil as claimed in claim 1 or 4, characterized in that the emulsifier preparation ratio is: engine oil 0.2-0.8, gasoline 0.2-0.8, kerosene 0.2-0.8, paraffin oil 0.2 -1.10, petroleum ether 0.5-1.0. 11. The Bacillus thuringiensis insecticide oil as claimed in claim 9, characterized in that the preparation ratio of the emulsifier is: 0.3-0.6 for motor oil, 0.3-0.6 for gasoline, 0.4-0.6 for kerosene, and 0.6-0.6 for paraffin oil. 0.9, petroleum ether. 12. The Bacillus thuringiensis insecticide oil as claimed in claim 1, characterized in that the preparation method of the Bacillus thuringiensis insecticide oil comprises: taking solvent oil and emulsifier in sequence, adding them to the container, After stirring evenly, weigh the original powder of Bacillus thuringiensis and add it, continue stirring until uniform, and seal it for storage.