CN1244275C - Crop powdery mildew control by osthole and its compound composition - Google Patents

Abstract

The invention discloses application of osthole single agent and compound composition thereof in the field of agriculture, in particular to application research aiming at preventing and controlling powdery mildew of crops. The invention provides an application of osthole in preventing and treating crop powdery mildew on one hand and a pesticide composition special for preventing and treating crop powdery mildew on the other hand, which comprises osthole and (B) a bactericide for inhibiting the synthesis of fungal ergosterol, such as one component selected from myclobutanil, triadimefon, tebuconazole or propiconazole, wherein the weight ratio of the two components is 1: 1-1: 20, and more preferably 1: 1-1: 10. It is especially suitable for preventing and treating powdery mildew of cucumber, pumpkin, strawberry, broad bean and grape.

Description

Crop powdery mildew control by osthole and its compound composition

Technical field:

The present invention relates to a single agent of osthole and its compound composition, especially its reasonable compounding with a fungicide that inhibits fungal ergosterol synthesis, such as myclobutanil, triadimefon, tebuconazole or propiconazole Later, it is used as an agricultural fungicide to control crop powdery mildew.

Background technique

The chemical name of the coumarin compound osthole (osthol) is 8-(3-methyl-2-butenyl) methyl ether sphenone, which is the main component of the umbelliferous plant Cnidium chinensis, and it is also Active ingredients extracted from the roots and rhizomes of Chinese angelica, Chinese angelica, Chinese angelica, celery, root bark of Rutaceae star anise, root bark of Rutaceae, single-leaf Rutaceae, etc. It has anti-cancer and anti-mutagenic effects in medicine, and insecticidal and antibacterial reports have been reported in agriculture, but its bactericidal spectrum and its synergistic effect and compounding ratio with other fungicides have not been reported yet.

For example, the inventor's previous patent CN01127083.7 "Application of osthole in agriculture" discloses the application of osthole in insecticide and sterilization, preservation of fruits and vegetables, and antisepsis of agricultural and livestock products. , only discloses the effective effect on Botrytis cinerea, garlic leaf spot, asparagus stem blight, pear ring blight, apple leaf blight, and its control effect on other crop pathogens is not disclosed, especially for The control and inhibitory effects of various crop powdery mildew bacteria are not disclosed.

Another previously published Chinese patent application CN1342412, titled "Application of Cnidium Fructus Extract in the Production of Agricultural Pesticides", relates to Cnidium Fructus Extract (mainly containing osthole) and its combination with other agricultural pesticides It is used in combination to prevent and control disease and insect pests, but these applications are limited to insecticide, and do not involve the application of this component in bactericidal aspect, let alone its application in the control of crop powdery mildew.

Powdery mildew is a worldwide widespread plant disease caused by Ascomycota subphylum Sclerotinia, Erysiphaceae Erysiphaceae. The losses caused by powdery mildew of melons and fruits, wheat, flowers, and forest trees are 5.8 to 6.5 billion U.S. dollars every year. After the mycelium invades the host plant, various haustoria (a form of mycelium) are formed in the body to absorb the nutrients of the crops, causing the crops to become diseased, and at the same time, a large number of conidia are formed to infect the crops again. The chemical control of powdery mildew of different crops can use the same fungicide, for example, myclobutanil is used to control wheat powdery mildew, and it is also used to control grape powdery mildew and strawberry powdery mildew.

Summary of the invention

The present invention is completed on the basis of the above-mentioned prior patent technology, through further research on the application of osthole single agent and its compound composition in the agricultural field, especially the application research on the control of crop powdery mildew. The purpose of the invention is to provide a single agent of osthole and its compound composition with other fungicides specially used for preventing and controlling crop powdery mildew.

Therefore, one aspect of the present invention provides the application of osthole in the prevention and treatment of crop powdery mildew.

Another aspect of the present invention provides the pesticide composition that is specially used in the prevention and treatment of crop powdery mildew, it comprises (A) osthole and (B) a kind of fungicide that inhibits fungal ergosterol synthesis, such as being selected from myclobutanil, triazole Ketone, tebuconazole or propiconazole is one of the four components, and the weight ratio of the two components is A/B=1:1 to 1:20, more preferably, 1:1 to 1:10 .

The invention is especially suitable for preventing and treating powdery mildew of cucumber, pumpkin, strawberry, broad bean and grape.

Detailed description of the invention

A single dose of osthole has good antibacterial effect on various crop powdery mildews. For example, its in vitro inhibitory effect is equivalent to that of the protective fungicide mancozeb. The efficacy is higher than that of mancozeb, and the effective dosage of powdery mildew control is 40-100μg/mL.

In addition, a single agent of osthole has good indoor activity against a variety of crop powdery mildew bacteria, and at the same time it is made into preparations, such as microemulsions, water-based agents, and wettable powders, and the control effect in the field can reach 70-90%.

The combination of osthole and other fungicides that inhibit fungal ergosterol synthesis, such as myclobutanil, triadimefon, tebuconazole or propiconazole, has the following obvious advantages:

1. It shows synergistic effect. This is because myclobutanazole, triadimefon, tebuconazole or propiconazole inhibit the synthesis of fungal ergosterol, so it is relatively slow-acting, while osthole is shown as white powder The direct killing of pathogenic hyphae and spores is quick-acting, and at the same time induces plant disease resistance. Therefore, the combination of the two shows synergy;

2. After compounding, compared with single dose, the dosage of osthole is reduced, and the effective dependence on plant resources is reduced. And because of the toxicity of myclobutanil (rat acute oral toxicity LD ₅₀ ♂＝1600mg/kg, ♀＝2290mg/kg; rabbit acute dermal toxicity LD ₅₀ ♂＞5000mg/kg, ♀＞5000mg/kg), triazole The toxicity of ketones (the acute oral toxicity LD ₅₀ of mice and rats is about 1000mg/kg; the acute percutaneous LD ₅₀ of rats > 5000mg/kg), the toxicity of tebuconazole (the acute oral toxicity LD ₅₀ of rats = 4000mg/kg kg, acute oral toxicity in mice ♂＝2000mg/kg, ♀＝2290mg/kg; acute percutaneous LD ₅₀ in rats >5000mg/kg), toxicity of propiconazole (acute oral toxicity in rats LD ₅₀ is about 1517mg/kg kg, mouse acute oral toxicity LD ₅₀ ♂=783mg/kg, ♀=509mg/kg; rat acute percutaneous LD ₅₀ >4000mg/kg) are higher than the toxicity of osthole (rat acute oral toxicity LD ₅₀ ♂＞5000mg/Kg, ♀＞5000mg/Kg; Rat Acute Percutaneous Toxicity LD ₅₀ ♂＞5000mg/Kg, ♀＞5000mg/Kg), after compounding, the dosage of chemical fungicides is reduced, thereby reducing the toxicity of the agent . Therefore, it is safer for crops to reduce its dosage.

3. After compounding, it can control the drug resistance of powdery mildew bacteria to fungal ergosterol synthesis inhibitors such as triadimefon, and at the same time reduce the dosage in the field, thus reducing the selection pressure of powdery mildew bacteria on such agents. According to reports, the resistance of cucumber powdery mildew bacteria to triadimefon has reached more than 60%, and triadimefon and myclobutanil, tebuconazole, and propiconazole are positive cross-resistance agents.

The osthole used in the present invention can be obtained by a known extraction process of osthole, such as the aforementioned prior art. The compounded myclobutanazole, triadimefon, tebuconazole and propiconazole are all commercially available pesticide products. The way to obtain it is not important, as long as it conforms to the proportioning range and application conditions of the present invention.

The following will describe in detail the proportion range, control object and compound specific examples of osthole and its compound composition with other pesticides that have synergistic effects. These contents are only for illustration and do not mean protection of the present invention. The scope is limited, and the active ingredients mentioned are all calculated according to their active ingredients.

Indoor formulation screening of osthole and other fungicides that inhibit fungal ergosterol synthesis

Tested pathogens and host crops

Cucumber powdery mildew (Phaerotheca fuliginea) was used as the test bacteria, and cucumber "Suchen 1" was used as the test host crop.

Test drug

Test agent is that 94.7% osthole (Jiangsu Academy of Agricultural Sciences provides) is dissolved in 95% ethanol, and it is standby to make 10 000 μ g/ml mother liquor; 95% myclobutanil, 95% triadimefon, 95% tebuconazole, 93% propiconazole (purchased from Fuda Agrochemical Co., Ltd., Jiangyin City, Jiangsu Province) was dissolved in acetone to prepare a 10000 μg/ml mother solution for future use.

Compound composition ratio design

Carry out proportioning design by preliminary test and the toxicity curve (bioassay method as described later) of the measured single agent, carry out compounding according to the compounding proportion of Table 1, each compounding agent is all dissolved in acetone, makes 10000 μ g /ml mother liquor for later use:

Table 1 Compound ratio range and compound examples of osthole and other fungicides Compound original drug A: B Compound ratio range A: B compound example Scale 1 Scale 2 Scale 3 94.7% Osthole/95% Myclobutanil 1:1～1:20 1/2 1/1 1/3 94.7% Osthole/95% Triadimefon 1/8 1/6 1/4 94.7% Osthole/95% Tebuconazole 1/10 1/7 1/4 94.7% Osthole/93% Propiconazole 1/7 1/5 1/3

Note: The proportions listed in the table are calculated according to the active ingredients of the medicine

Then the above-mentioned original medicine and compound preparation are diluted into a series of concentration medicinal liquids according to Table 2 for subsequent use.

Table 2 Serial dilution concentration gradient of each medicament potion Dilution Concentration Gradient (ug/ml) 94.7% Osthole 200 100 50 25 12.5 95% myclobutanil 50 25 12.5 6.25 3.125 95% Triadimefon 40 20 10 5 2.5 95% Tebuconazole 10 5 2.5 1.25 0.625 93% propiconazole 20 10 5 2.5 1.25 94.7% Osthole/95% Myclobutanil 50 25 12.5 6.25 3.125 94.7% Osthole/95% Triadimefon 20 10 5 2.5 1.25 94.7% Osthole/95% Tebuconazole 10 5 2.5 1.25 0.625 94.7% Osthole/93% Propiconazole 20 10 5 2.5 1.25

In addition, clear water was used as a blank control to determine the susceptibility to cucumber powdery mildew.

Bacteria solution preparation :

The powdery mildew fungi collected from the field were scraped off, and a conidia suspension was formulated with sterile water at a concentration of 10-20 conidia per field of view under a microscope of 10×10 times.

Measurement conditions and standards

Spray evenly the medicinal solutions of different treatment concentrations on the fresh cucumber leaves, and after 24 hours, drip 3 points on each leaf, each point of 5ul spore liquid. After 4 days, the disease progression was recorded, and the disease index and control effect were calculated.

Grading standards for disease index investigation:

Grade 0: no lesions;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: Lesion area accounts for 6% to 10% of the entire leaf area;

Grade 5: Lesion area accounts for 11% to 25% of the entire leaf area;

Grade 7: The lesion area accounts for 26% to 50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

calculation method

Calculate the control effect according to the formula, use the percentage and probability value comparison table to convert the control effect into a probability value, and at the same time convert the dose concentration into a logarithmic value, and use the dose logarithm value (x) and probability value (y) to obtain the toxicity formula Y=a +bx, calculate the correlation coefficient r and the inhibitory medium concentration EC ₅₀ .

Calculate the interaction of the mixture according to Wadley's method, and analyze the effect of the mixture with the SR value of the synergy coefficient:

Control effect (%)=[(control disease finger-treatment disease finger)/control disease finger]×100%,

EC _{50 (theoretical value)} = (c+d)/[c/EC _50(C) +d/EC _50(D) ]

SR＝EC _{50(theoretical value)} /EC _{50(actual value)}

C and D represent the two components in the mixture respectively

c and d respectively represent the proportions of the two single agents in the mixture

EC _{50 (actual value)} is the actual measured value of a specific mixture)

SR ≥ 1.5 means that the two single agents have a synergistic effect after mixing

SR ≤ 0.5 means that the two single agents have an antagonistic effect after mixing

0.5≤SR≤1.5 means that the two single agents have an additive effect after mixing.

results and analysis

The control effects of different concentrations of osthole and fungicides that inhibit fungal ergosterol synthesis and their complexes on cucumber powdery mildew are shown in Tables 3 and 4. See Table 5 for their virulence regression equation y=a+bx, correlation coefficient r, effective inhibitory medium concentration EC ₅₀ (ug/mL) and mixture synergy coefficient SR against cucumber powdery mildew.

As can be seen from Table 5, the fungicides that inhibit fungal ergosterol synthesis have higher activity against cucumber powdery mildew bacteria than osthole under living conditions; the synergistic coefficients after compounding are all greater than 1.5, which has good synergistic effect effect. In addition, although the activity of these triazole fungicides is relatively high, problems such as phytotoxicity and drug resistance are prone to occur in the field, while osthole also has good activity against cucumber powdery mildew under living conditions, and the effects of these two types of fungicides The mechanism is different, and there is no problem of cross-resistance, so the mixture of these two types of agents can be used to alleviate the resistance problem of such systemic fungicides and prolong the service life of the agents.

Table 3 In vitro control effect of five kinds of fungicide single agent on cucumber powdery mildew 95% myclobutanil Treatment concentration (μg/mL) 50 25 12.5 6.25 3.12 Control(0) Sick finger ^* 6.3 13.2 16.2 26.2 29 58.9 Anti-efficacy (%) 89 78 72 56 51 / 95% Triadimefon Treatment concentration (μg/mL) 40 20 10 5 2.5 Control(0) Sick finger ^* 58.9 14.4 17.9 30.1 34.4 58.9 Anti-efficacy (%) 85.6 75.6 69.6 48.9 41.6 / 95% Tebuconazole Treatment concentration (μg/mL) 10 5 2.5 1.25 0.625 Control(0) Sick finger ^* 7 12.9 15.3 25.1 27.8 58.9 Anti-efficacy (%) 8.1 78.1 74 57.4 52.7 / 93% propiconazole Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control(0) Sick finger ^* 5.1 11.1 14.9 21.4 26.9 58.9 Anti-efficacy (%) 91.3 81.2 74.7 63.7 54.3 / Osthole Treatment concentration (μg/mL) 50 25 12.5 6.25 3.12 Control(0) Sick finger ^* 12.3 15.3 twenty one 26.3 34 58.9 Anti-efficacy (%) 79 74 64 55 42 /

^* average of 4 replicates

                                                                                 

In Vitro Control Effect on Cucumber Powdery Mildew 94.7% osthole: 95% triadimefon = 1:6 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control(0) Sick finger ^* 7 11.2 14.2 22.2 27 58.9 Anti-Efficacy ^* (%) 88.9 81 75.9 62.3 54.2 / 94.7% osthole: 95% triadimefon = 1:8 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control(0) Sick finger ^* 6 9.2 12.7 20.2 25.7 58.9 Anti-Efficacy ^* (%) 89.8 84.4 78.4 65.7 56.4 / 94.7% osthole: 95% triadimefon = 1:4 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control(0) Sick finger ^* 7.6 12.7 16.1 24.3 29.8 58.9 Anti-Efficacy ^* (%) 87.1 78.4 72.7 58.7 50.6 / 94.7% osthole: 95% tebuconazole = 1:10 Treatment concentration (μg/mL) 10 5 2.5 1.25 0.625 Control(0) Sick finger ^* 5.3 9.1 12.3 18.9 23.3 58.9 Anti-Efficacy ^* (%) 91 84.6 79.1 67.9 60.4 / 94.7% osthole: 95% tebuconazole = 1:7 Treatment concentration (μg/mL) 10 5 2.5 1.25 0.625 Control(0) Sick finger ^* 5.9 10.2 13.7 20.3 26.1 58.9 Anti-Efficacy ^* (%) 90 82.7 76.7 65.5 55.7 / 94.7% osthole: 95% tebuconazole = 1:4 Treatment concentration (μg/mL) 10 5 2.5 1.25 0.625 Control(0) Sick finger ^* 6.3 11.6 15.4 20.3 29.2 58.9 Anti-Efficacy ^* (%) 89.3 80.3 73.9 65.5 50.4 / 94.7% osthole: 95% myclobutanil = 1:1 Treatment concentration (μg/mL) 50 25 12.5 6.25 3.125 Control(0) Sick finger ^* 6.7 10.3 14.1 20.1 27.9 58.9 Anti-Efficacy ^* (%) 88.6 82.5 76.1 65.9 52.8 / 94.7% osthole: 95% myclobutanil = 1:2 Treatment concentration (μg/mL) 50 25 12.5 6.25 3.125 Control(0) Sick finger ^* 5.4 9.5 12.9 19.3 25.1 58.9 Anti-Efficacy ^* (%) 90.8 83.9 78.1 67.2 57.4 /

94.7% osthole: 95% myclobutanil = 1:3 Treatment concentration (μg/mL) 50 25 12.5 6.25 3.125 Control (0) Sick finger ^* 4.9 8.4 11.7 17.9 23.1 58.9 Anti-Efficacy ^* (%) 91.7 85.7 80.1 69.6 60.8 / 94.7% osthole: 93% propiconazole = 1:7 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control (0) Sick finger ^* 5.1 9.1 12.5 18.8 23.7 58.9 Anti-Efficacy ^* (%) 91.3 84.6 78.8 68.1 60.8 / 94.7% osthole: 93% propiconazole = 1:5 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control (0) Sick finger ^* 5.9 10.7 13.6 20.1 25.2 58.9 Anti-Efficacy ^* (%) 90 81.8 76.9 65.8 57.2 / 94.7% osthole: 93% propiconazole = 1:3 Treatment concentration (μg/mL) 20 10 5 2.5 1.25 Control (0) Sick finger ^* 6.7 11.8 14.7 21.3 27.7 58.9 Anti-Efficacy ^* (%) 88.6 80 70.6 58.1 50.9 /

^* Average of 4 replicates

Table 5 Screening results of compound indoor formula of osthole and triazole fungicides potion Virulence regression equation Correlation coefficient r Actual effective middle concentration E _C50 (μg/ml) Theoretical effective medium concentration E _C50 (μg/ml) Synergistic coefficient SR 94.7% Osthole y=18.6767+32x 0.97 108.6 / / 95% Triadimefon y=26.45+13.14x 0.988 8.456 / / 94.7% osthole: 95% triadimefon = 1:8 y=24.457+16.6x 0.968 5.608 9.42 1.68 94.7% osthole: 95% triadimefon = 1:6 y=29.371+17.24x 0.996 4.058 9.739 2.4 94.7% osthole: 95% triadimefon = 1:4 y=23.34+17.23x 0.975 5.457 10.368 1.9 95% Tebuconazole y=19.35+23.24x 0.978 7.96 / / 94.7% osthole: 95% tebuconazole = 1:10 y=18.65+33.16x 0.97 4.551 8.692 1.91 94.7% osthole: 95% tebuconazole = 1:7 y=20.9+11.5x 0.99 3.864 9 2.33 94.7% osthole: 95% tebuconazole = 1:4 y=17.94+8.3x 0.959 5.552 9.771 1.76 95% myclobutanil y=31.26+11.33x 0.964 11.36 / / 94.7% osthole: 95% myclobutanil = 1:1 y=17.22+7.659x 0.972 18.42 28.18 1.53 94.7% osthole: 95% myclobutanil = 1:2 y=15.49+20.13x 0.98 14.06 25.3 1.8 94.7% osthole: 95% myclobutanil 1:3 y=19.56+23.56x 0.966 11.82 20.57 1.74 93% propiconazole y=16.584+27.4x 0.986 7.132 / / 94.7% osthole: 93% propiconazole = 1:7 y=16.74+15.26x 0.979 4.562 8.075 1.77 94.7% osthole: 93% propiconazole = 1:5 y=16.371+7.25x 0.976 3.505 8.447 2.41 94.7% osthole: 93% propiconazole = 1:3 y=27.924+8.73x 0.985 4.797 9.306 1.94

Field efficacy test of compound preparation of osthole and other fungicides

According to the "Guidelines for Field Efficacy Tests of Pesticides" edited by the Pesticide Control Institute of the Ministry of Agriculture, the field efficacy tests of compound formulations on various crop powdery mildews were designed.

Test design

The specific test design is shown in Table 6:

Table 6 field efficacy test agents Test crops Control object Test drug Control drug Cucumber variety is Jinyou 4 Cucumber powdery mildew (E.cucurbitacearum) 1% osthole EW 50% Familia double WP 20% Triadimefon EC 5% Osthole · Triadimefon SE 7% Osthole Triadimefon SE 9% Osthole Triadimefon SE Jufeng Grape powdery mildew (P.pyri) 1% osthole EW 10% Segao WG 6% Tebuconazole ME 5% Osthole Tebuconazole SE 8% Osthole Tebuconazole SE 11% Osthole Tebuconazole SE Broad bean variety Nantong Qingpi Vicia powdery mildew (P.pisi) 1% osthole EW 40% Fuxing EC 12.5% Myclobutanil WP 12% Osthole · Myclobutanil SE 16% Osthole · Myclobutanil SE 10% Osthole · Myclobutanil SE Toyoka Strawberry powdery mildew (S.rosae) 1% osthole EW 10% Segao WG 25% propiconazole EC 4% Osthole · Propiconazole SE 6% Osthole Propiconazole SE 8% Osthole · Propiconazole SE The variety of pumpkin is Dongsheng Pumpkin powdery mildew (E.cucurbitacearum) 1% osthole EW 15% Triadimefon W 25% propiconazole EC 4% Osthole · Propiconazole SE 6% Osthole Propiconazole SE 8% Osthole · Propiconazole SE

Test drug:

1% osthole EW250×, Jiangsu Academy of Agricultural Sciences, the effective concentration is 40ug/ml.

12.5% myclobutanil WP1500×, produced by Zhangjiagang Qizhou Pesticide Chemical Co., Ltd., the effective concentration is 83.3ug/ml.

2% osthole · myclobutanil SE500× (active ingredient: 1% osthole + 1% myclobutanil), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 20ug/ml myclobutanil.

3% osthole · myclobutanil SE500× (active ingredient: 1% osthole + 2% myclobutanil), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 40ug/ml myclobutanil.

4% osthole · myclobutanil SE500× (active ingredient: 1% osthole + 3% myclobutanil), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 60ug/ml myclobutanil.

25% propiconazole EC1200×, produced by Changzhou Fengdeng Pesticide Factory, the effective concentration is 208.3ug/ml.

4% osthole · propiconazole SE500× (active ingredient: 1% osthole + 3% propiconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 60ug/ml propiconazole.

6% osthole · propiconazole SE500× (active ingredient: 1% osthole + 5% propiconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 100ug/ml propiconazole.

8% osthole · propiconazole SE500× (active ingredient: 1% osthole + 7% propiconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 140ug/ml propiconazole.

6% Tebuconazole ME250×, produced by Changzhou Fengdeng Pesticide Factory, the effective concentration is 240ug/ml.

5% osthole·tebuconazole SE500× (active ingredient: 1% osthole + 4% tebuconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 80ug/ml tebuconazole.

8% osthole·tebuconazole SE500× (active ingredient: 1% osthole + 7% tebuconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 140ug/ml tebuconazole.

11% osthole·tebuconazole SE500× (active ingredient: 1% osthole + 10% tebuconazole), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 200ug/ml tebuconazole.

20% Triadimefon EC1000× (produced by Changzhou Fengdeng Pesticide Factory), the effective concentration is 200ug/ml

5% osthole · triadimefon SE500× (active ingredient: 1% osthole + 4% triadimefon), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 80ug/ml triadimefon.

7% osthole · triadimefon SE500× (active ingredient: 1% osthole + 6% triadimefon), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 120ug/ml triadimefon.

9% osthole · triadimefon SE500× (active ingredient: 1% osthole + 8% triadimefon), Jiangsu Academy of Agricultural Sciences, the effective concentration is 20ug/ml osthole + 160ug/ml triadimefon.

Comparative drug:

50% thiram WP 500×, produced by Tianjin Xingguo Pesticide Factory, the effective concentration is 1000ug/ml.

10% Shigao WG1000×, produced by Shanghai Syngenta (China) Investment Co., Ltd., with an effective concentration of 100 μg/ml.

15% Triadimefon WP800×, produced by Yangzhou Dongbao Pesticide Chemical Co., Ltd., with an effective concentration of 187.5 μg/ml.

40% Fuxing EC9000×, produced by Jiangsu Jiannong Pesticide Chemical Co., Ltd., the effective concentration is 44.4μg/ml

Blank control: clear water.

Among them, SE means water suspoemulsion, EW means water emulsion, WP means wettable powder, WG means water-soluble granule.

Community settings

According to the results of the preliminary test, a series of concentration gradients were set up to carry out the plot test, each plot was 20 square meters, and each treatment was repeated 4 times. Applying pesticides at the initial stage of disease, using a Gongnong-16 artificial knapsack sprayer in the test, to spray the front and back sides of the leaves just before dripping, and the dosage of the pesticide solution is 50 kg/mu.

Disease investigation:

Investigate the disease base before the first spraying, investigate the control effect after the last spraying before each spraying, and investigate 10 days after the last spraying, that is, take samples at five points in each plot, investigate 3 plants at each point, and investigate the fruit When inspecting 3 to 5 leaves per plant, when investigating leaves, take three leaves from the upper, middle and lower parts of each plant for investigation, and classify according to the percentage of lesion area in the entire leaf area.

Grading standards:

Grade 0: no lesions;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: Lesion area accounts for 6% to 15% of the entire leaf area;

Grade 5: Lesion area accounts for 16% to 25% of the entire leaf area;

Grade 7: The lesion area accounts for 26% to 50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

Drug efficacy calculation method:

Control effect (%)=(1-CK ₀ ×Pt ₁ /CK ₁ ×Pt ₀ )×100%

(CK ₀ and CK ₁ represent the diseased fingers before and after the control drug respectively, and Pt ₀ and Pt ₁ represent the diseased fingers before and after the drug in the treatment area respectively)

Other effects on crops

Observe whether there is phytotoxicity, and record the degree of phytotoxicity if there is phytotoxicity:

-: no harm;

+: Slight phytotoxicity, no effect on crop growth;

++: Obvious phytotoxicity, recoverable, and will not cause crop yield reduction;

+++: High phytotoxicity, affecting the normal growth of crops, causing a certain degree of loss to crop yield and quality;

++++: Severe phytotoxicity, stunted crop growth, serious loss of yield and quality.

test results

Field control effects of various pesticides on crop powdery mildew

See Table 7 for the field control effects of various pesticides on crop powdery mildew. For the control of ^broad bean powdery mildew, 1% osthole EW250×, 12.5% myclobutanil WP1500×, 2% osthole·myclobutanazole SE 500×, The average control effects of 3% osthole·myclobutanazole SE 500×, 4% osthole·myclobutanazole SE 500× were 65.4%, 75.3%, 88.6%, 87.4% and 89.3%, respectively. The average control effect of 40% Fuxing EC9000× was 84.7%. The results of variance analysis showed that the treatment agent 2% osthole·myclobutanazole SE 500×, 3% osthole·myclobutanazole SE 500×, 4% osthole·myclobutanazole SE 500× and the control drug 50% There was no significant difference between the control effects of thiram WP 500×, but the difference between the control effects of two single doses of 1% osthole EW250× and 12.5% myclobutanil WP1500× reached a very significant level.

Control cucumber powdery mildew with ¹ % osthole EW250×, 20% triadimefon EC1000×, 5% osthole·triadimefon SE500×, 7% osthole·triadimefon SE500×, 9% per 667m2 The average control effects of osthole · triadimefon SE500× and other agents were 67.7%, 71.8%, 84.5%, 81.9%, 88.8% respectively, and the control effect of 50% thiram WP 500× of the control agent was 85.3%. The results of variance analysis showed that the treatment agent 5% osthole·triadimefon SE500×, 7% osthole·triadimefon SE500×, 9% osthole·triadimefon SE500× and the control agent 50% thiram WP There was no significant difference between the control effects of 500×, but the difference between the control effects of two single doses of 1% osthole EW250× and 20% triadimefon EC1000× reached a very significant level.

Control grape powdery mildew with ¹ % osthole EW250×, 6% tebuconazole ME250×, 5% osthole·tebuconazole SE500×, 8% osthole·tebuconazole SE500×, 11% per 667m2 The average control effects of osthole·tebuconazole SE500× and other agents were 67.3%, 75.9%, 87.3%, 89.3%, and 87.9%, respectively, and the control effect of the control agent 10% Shigao WG1000× was 88.3%. The results of variance analysis showed that the control effect of 5% osthole·tebuconazole SE500×, 8% osthole·tebuconazole SE500×, 11% osthole·tebuconazole SE500× was higher than that of the control agent 10% Shigao There was no significant difference between the control effects of WG1000×, and the difference between the control effects of two single doses of 1% osthole EW250× and 6% tebuconazole ME250× reached a very significant level.

Control strawberry powdery mildew with ¹ % osthole EW250×, 25% propiconazole EC1200×, 4% osthole·propiconazole SE500×, 6% osthole·propiconazole SE500×, 8% per 667m2 The average control effects of osthole·propiconazole SE500× were 70%, 71.25%, 78.2%, 80.2%, 84.7% respectively, and the control effect of 10% Shigao WG1000× was 76.7%. The results of variance analysis showed that the control effect of 4% osthole·propiconazole SE500×, 6% osthole·propiconazole SE500×, 8% osthole·propiconazole SE500× was comparable to that of a single dose of 1% osthole There is a very significant difference between the control effect of EW250× and the control effect of 25% propiconazole EC1200×; the control effect of 4% osthole·propiconazole SE500× is different from that of the control drug 10% Shigao WG1000× There was no significant difference between them; there was a significant difference between the control effects of 6% osthole·propiconazole SE500×, 8% osthole·propiconazole SE500× and the control effect of 10% Shigao WG1000×.

Control pumpkin powdery mildew with ¹ % osthole EW250×, 25% propiconazole EC1200×, 4% osthole·propiconazole SE500×, 6% osthole·propiconazole SE500×, 8% per 667m2 The average control effects of osthole·propiconazole SE500× were 65.7%, 80.3%, 85.7%, 84.1%, 85.2% respectively, and the control effect of the control drug 15% triadimefon WP800× was 72.3%. The results of variance analysis showed that the control effect of 4% osthole·propiconazole SE500×, 6% osthole·propiconazole SE500×, 8% osthole·propiconazole SE500× was comparable to that of the control agent 15% triazole There is a very significant difference between the control effect of ketone WP800× and the control effect of single dose 1% osthole EW250×, and the difference between the control effect of 25% propiconazole EC1200× is significant.

Pharmacy evaluation

Each compound agent has good control effect on crop powdery mildew, the control effect can reach about 80%, which is higher than the control effect of each control single agent, and can effectively prevent the damage of powdery mildew to various crops.

According to the systematic observation after application, no phytotoxicity occurred during the test period, and each test agent had no adverse effect on crop growth.

Table 7 Field efficacy test results of osthole and triazole fungicide compound preparations for controlling powdery mildew of various crops treatment agent Control object Predrug Condition Index ^* 7 days after the third dose Sick finger ^* Disease index growth rate ^* (%) Anti-Efficacy ^* (%) Phytotoxicity 1% Osthole EW250× Broad bean powdery mildew 9.7 15.6 60.8 65.4Cc - 12.5% myclobutanil WP1500× 8.8 10.1 14.8 75.3Bb - 2% Osthole · Myclobutanil SE500× 9.1 4.8 -47.3 88.6Aa - 3% Osthole Myclobutanil SE500× 9.5 5.6 -41 87.4Aa - 4% Osthole · Myclobutanil SE500× 8.7 4.3 -50.6 89.3Aa - 40% Fuxing EC9000× 9.3 6.6 -29 84.7 Aab - Clear water control 9.4 43.7 364.9 / - 1% Osthole EW250× Cucumber powdery mildew 10.5 17.96 71 67.7Cc - 20% Triadimefon EC1000× 9.9 14.8 49.5 71.8 BBC - 5% Osthole Triadimefon SE500× 10.3 8.5 -17.5 84.5Aa - 7% Osthole Triadimefon SE500× 8.5 8.1 -4.7 81.9 Aab - 9% Osthole Triadimefon SE500× 7.3 4.3 41.1 88.8Aa - 50% Familia WP 500× 8.1 6.3 -22.2 85.3Aa - Clear water control 9.5 50.3 429.5 / - 1% Osthole EW250× grape powdery mildew 7.9 11.3 43 67.3Cc - 6% Tebuconazole ME250× 7.9 8.3 5.1 75.9Bb - 5% Osthole · Tebuconazole SE500× 8.6 17.4.89 -44.2 87.3Aa - 8% Osthole Tebuconazole SE500× 9.6 18.84.5 -53.1 89.3Aa - 11% Osthole Tebuconazole SE500× 8.2 14.23.5 -57.3 87.9Aa - 10% Shigao WG1000× 9.1 4.6 -49.5 88.3Aa - Clear water control 7.4 32.3 336.5 / - 1% Osthole EW250× Strawberry powdery mildew 10.3 15.6 51.46 70. BBC - 25% propiconazole EC1200× 9.7 14.1 45.36 71.25Bbc - 4% Osthole·Propiconazole SE500× 9.6 10.6 10.42 78.2Ab - 6% Osthole·Propiconazole SE500× 8.9 8.9 0 80.2 Aab - 8% Osthole·Propiconazole SE500× 10.1 7.8 -22.77 84.7 Aab - 10% Shigao WG1000× 9.5 11.2 17.89 76.7Ab - Clear water control 10.6 53.6 405.66 / - 1% Osthole EW250× Pumpkin Powdery Mildew 6.3 15.7 149.2 65.7Cc - 25% propiconazole EC1200× 6.1 8.7 42.6 80.3Ab - 4% Osthole·Propiconazole SE500× 6.5 6.8 4.6 85.7Aa - 6% Osthole·Propiconazole SE500× 6.79 7.9 16.3 84.1Aa - 8% Osthole·Propiconazole SE500× 6.5 7 7.7 85.2Aa - 15% Triadimefon WP800× 5.9 11.9 101.7 72.3Bb - Clear water control 5.2 37.8 626.9 / -

^* Average of 4 replicates

safety test

Among the above-mentioned other fungicides compounded with osthole, the dosage is half or less than half of the conventional dosage of a single agent, so its toxicity to humans and animals and the safety of crops meet the requirements, and in the above formula , the highest content of osthole is 1%, and the results of the toxicity test by the recognized agency show that 1% osthole water emulsion belongs to the slightly toxic pesticide:

Rat acute oral toxicity LD50♂＞5000mg/Kg, ♀＞5000mg/Kg;

Rat acute dermal toxicity LD50♂＞5000mg/Kg, ♀＞5000mg/Kg;

Rabbit eye irritation score is 0 (1h), 0 (1:100) dilution after 48h, no irritation to eyes;

Rabbit eye irritation score is 0 (4h), non-irritating to the skin.

Embodiment (osthole and other fungicide compound examples):

Embodiment 1: 2% osthole·myclobutanazole SE 500× (active ingredient: 1% osthole+1% myclobutanil, effective concentration is 20 μg/ml osthole+20 μg/ml myclobutanil) for the prevention and treatment of In the early stage of grape powdery mildew, use 100ml per mu, and later as the leaves increase, the dosage will reach 200-300ml per mu

Embodiment 2: 4% osthole·myclobutanazole SE 500× (active ingredient: 1% osthole+3% myclobutanil, effective concentration is 20 μg/ml osthole+60 μg/ml myclobutanil) prevention and treatment For broad bean powdery mildew, 100ml per mu is used in the early stage of the disease, and 200-300ml per mu as the leaves increase in the later stage.

Embodiment 3: 8% osthole·tebuconazole SE500 * (active ingredient: 1% osthole+7% tebuconazole, effective concentration is 20 μ g/ml osthole+140 μ g/ml tebuconazole to control cucumber, Pumpkin, zucchini, melon, and strawberry powdery mildew were sprayed 500 times at the early stage of the disease, 100ml per mu, once every 5-7 days. Later, as the leaves increased, the dosage reached 200-300ml per mu.

Embodiment 4: 7% osthole · triadimefon SE500 * (active ingredient: 1% osthole+6% myclobutanil, effective concentration is 20 μ g/ml osthole+120 μ g/ml triadimefon. Prevention and treatment grape For powdery mildew, start spraying at the early stage of the disease, use 100ml per mu, and spray once every 5-7 days. Later, as the leaves increase, the amount of spraying per mu will reach 200-300ml.

Embodiment 5: 8% osthole · propiconazole SE500 * (active ingredient: 1% osthole+7% myclobutanil, effective concentration is 20 μ g/ml osthole+140 μ g/ml triadimefon. Control strawberry , Pumpkin powdery mildew, start spraying at the early stage of the disease, use 100ml per mu, spray once every 5 to 7 days. Later, as the leaves increase, the dosage per mu will reach 200 to 300ml.

Claims (2)

1. the application of Osthole on the control crop powdery mildew.

2. application as claimed in claim 1, wherein said crop are cucumber, pumpkin, strawberry, broad bean and grape.