CN101455203B - Compound composition of osthole and carbendazim and application thereof - Google Patents
Compound composition of osthole and carbendazim and application thereof Download PDFInfo
- Publication number
- CN101455203B CN101455203B CN2008102498764A CN200810249876A CN101455203B CN 101455203 B CN101455203 B CN 101455203B CN 2008102498764 A CN2008102498764 A CN 2008102498764A CN 200810249876 A CN200810249876 A CN 200810249876A CN 101455203 B CN101455203 B CN 101455203B
- Authority
- CN
- China
- Prior art keywords
- carbendazim
- osthole
- complex composition
- apple
- compound composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 239000006013 carbendazim Substances 0.000 title claims abstract description 52
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 title claims abstract description 51
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 title claims abstract description 50
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical group C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 title claims abstract description 50
- 239000000203 mixture Substances 0.000 title claims abstract description 37
- 150000001875 compounds Chemical class 0.000 title abstract description 11
- 239000000375 suspending agent Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 16
- 241000220225 Malus Species 0.000 description 15
- 230000003449 preventive effect Effects 0.000 description 14
- 235000013399 edible fruits Nutrition 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 7
- 239000003899 bactericide agent Substances 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000000575 pesticide Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 231100000419 toxicity Toxicity 0.000 description 6
- 230000001988 toxicity Effects 0.000 description 6
- 239000003905 agrochemical Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 235000010603 pastilles Nutrition 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000001018 virulence Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 244000296102 Selinum monnieri Species 0.000 description 2
- 235000019084 Selinum monnieri Nutrition 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 206010023683 lagophthalmos Diseases 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000002420 orchard Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241001254604 Angelica pubescens Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000239080 Botryosphaeria berengeriana f. sp. pyricola Species 0.000 description 1
- 241000125183 Crithmum maritimum Species 0.000 description 1
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000702321 Microvirus Species 0.000 description 1
- 244000303512 Peucedanum ostruthium Species 0.000 description 1
- 235000007155 Peucedanum ostruthium Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- -1 carbendazim compound Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 231100000584 environmental toxicity Toxicity 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000009781 safety test method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001550 time effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- BKOOMYPCSUNDGP-UHFFFAOYSA-N trimethyl-ethylene Natural products CC=C(C)C BKOOMYPCSUNDGP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of a compound composition of osthole and carbendazim in the agricultural field, in particular to application research on prevention and treatment of apple ring rot. The invention provides application of osthole and a compound composition of osthole and carbendazim in preventing and treating apple ring spot, wherein the weight ratio of the osthole and the carbendazim in the compound composition is 1: 25-40, the preferred ratio is 1: 30-40, and the optimal ratio is 1: 35. It is prominent in the aspect of preventing and treating apple ring spot.
Description
One, technical field
The present invention relates to Osthole and complex composition thereof, especially the composite back of Osthole and carbendazim is applied to prevent and treat ring rot of apple as bactericide, belongs to the complex composition technical field of bactericide.
Two, background technology
Ring rot of apple is that disease wide, that generation is heavy takes place in the apple orchard, can cause a large amount of decayed fruits.Ring spot took place since August, before gathering, continued to increase.The decayed fruit rate is generally 20～50%, and heavy person can reach 70～90%, directly influences orchard worker's income.
The coumarin compound Osthole is a botanical pesticide; Chemical name is 8-(3-methyl-2-butene base) methyl ether San ketone; It is a kind of samphire cnidium monnieri (Cnidium monnieri (L.) Cuss) plant extracts that derives from; Also be present in Imperatoria ostruthium, the root of justifying Radix Angelicae Sinensis, Angelica pubescens, bolt wing celery, the rhizome, the method for distilling of multiple maturation is arranged.Originally be applied in medically, found on agricultural, to can be used as insecticidal bactericide (license publication number: CN 1119930C, CN 1332603C, CN 1244275C, CN 1586198A, CN 100409747C, CN 100356851C etc.) afterwards.But Osthole and also do not appear in the newspapers aspect the ring rot of apple preventing and treating with the complex composition of carbendazim.
Carbendazim is a kind of efficient, low toxicity, the systemic fungicide that is widely used in the agricultural production, and the control spectrum is wide, has solved the environmental toxicity problem of other bactericide.But because carbendazim height specialization, action site is single, adds and produces medium-term and long-term single use, causes pesticide resistance colony constantly to occur, and induces germ to develop immunity to drugs easily.It is being main bactericide kind aspect the apple safety in production disease control, but recent years, the phenomenon of downslide rapidly appearred in preventive effect.
Agricultural chemicals is faced with huge predicament at present.The novel pesticide development cost is high, the cycle is long, and can catch up with the speed that the germ pesticide resistance produces never, and it is extremely urgent to solve pesticide resistance.In the agricultural production actual mechanical process, different agricultural chemicals wait measure to be difficult to really play obvious effects by turns; Shift of crops is unpractical for perennial apple.Different classes of pesticide species carries out composite, be the prevention or solve drug-fast common methods, also be the important channel that obtains vitality like some old kind agricultural chemicals such as carbendazim once more, make full use of existing bactericide resource.
Three, summary of the invention
The present invention is on the basis of above-mentioned prior art; The application of complex composition in agriculture field through further investigation Osthole and carbendazim; The research of especially using to ring rot of apple control is accomplished, and purpose is to provide and is exclusively used in the Osthole of preventing and treating ring rot of apple and the complex composition of carbendazim.
The technical scheme that the present invention adopts is following:
Complex composition of the present invention contains two kinds of compositions of Osthole and carbendazim, and both weight ratios are 1: 25～40, and preferred proportion is 1: 30～40, and optimal proportion is 1: 35;
The application under diluting 400 times, 500 times of the complex composition of Osthole of the present invention and carbendazim prevents and treats ring rot of apple and has preventive effect preferably.
Osthole of the present invention and carbendazim complex composition have the following advantages:
1, has synergistic effect.Osthole and carbendazim are to have synergistic effect in 1: 25～40 ratio ranges, and wherein proportioning synergy in 1: 35 is maximum, and coefficient of synergism is 3.34;
2, high security.Osthole is a plant extracts, has Environmental compatibility; The 36% Osthole carbendazim suspending agent that we make contains 35% carbendazim; Than carbendazim content is that 50% city pin product has reduced 15 percentage points; Thereby further reduced the medicament toxicity of carbendazim, so complex composition of the present invention is safer to crop, environment and fresh fruit eater;
3, preventive effect is remarkable.We process 36% Osthole carbendazim suspending agent and in field control effectiveness test, have outstanding performance; Dilute 400 times, 500 times processing in the preventive effect of picking time all more than 85%; The preventive effect of storage period all is significantly higher than the preventive effect of 500 times of 200 times of 1% osthole water-emulsion agent and 50% carbendazim all more than 82%.36% Osthole carbendazim suspending agent makes old kind fungicide carbendazim bring into play remarkable effect once more aspect the ring rot of apple preventing and treating;
4, reduced the germ risk that develops immunity to drugs.Of advantage 3, complex composition carbendazim content is than 15 percentage points of the minimizings of market sale, and this has significantly alleviated the selection pressure of ring rot of apple bacterium to carbendazim; The Osthole that contains in the complex composition has a plurality of action sites, can avoid germ that it is developed immunity to drugs;
5, reduced dependence to plant resources.Of advantage 3; The 36% Osthole carbendazim suspending agent field dilution that contains 1% Osthole has obtained good preventive effect for 400 times, 500 times; And preventive effect picking time of 200 times of 1% osthole water-emulsion dilution agents is 76.31%, storage period preventive effect all be lower than 65%.
Four, embodiment
The present invention is elaborated with following instantiation, but limitation of the present invention absolutely not.Percentage all is weight percentage in all proportionings.
Embodiment 1
Toxicity Determination and compound proportion screening
1.1 supply the reagent agent
94.7% Osthole, academy of agricultural sciences, Jiangsu Province food quality safety research institute provides.Be dissolved in 95% alcohol, it is subsequent use to be made into the 10000mg/L mother liquor.
98% carbendazim, Huayang Agricultural Chemial Group Co Ltd, Shandong provides.It is subsequent use to be made into the 10000mg/L mother liquor with 0.1mol/L hydrochloric acid.
The content of producers such as rich natural products Co., Ltd of China city far away development in science and technology Co., Ltd, Shaanxi standing grain, the three-dimensional Bioisystech Co., Ltd in Xi'an is the requirement that 10～90% Osthole product all meets the used Osthole of complex composition among the present invention; And with the carbendazim of fructus cnidii complex be commercially available Pesticidal products, as long as it meets compound proportion and application conditions among the present invention.
1.2 strains tested and host plant
Sick fruit goes up the separation acquisition to ring rot of apple bacterium (Botryosphaeria berengeriana f.sp.pyricola) from the field, and the first generation bacterial strain that the single cell culture purifying obtains is preserved subsequent use in 4 ℃ of refrigerators.
1.3 experimental technique
The biomass growth rate method (Mycelium growth rate test) that adopts FAO to recommend.
1.3.1 the preparation that pastille is dull and stereotyped
1.3.1.1 composite reagent scheme
Table 1 Osthole and carbendazim compound proportion
Each medicine series diluted concentration gradient of table 2
1.3.1.2 the preparation that pastille is dull and stereotyped
Drug concentration gradient according to 1.3.1.1 sets adds medicament in the PDA medium that quantitatively dissolves, and processes the pastille flat board, is blank with pastille flat board not, and every concentration repeats 3 times.
1.3.2 connecing bacterium cultivates
Beat along cultured colony edge with diameter 0.6cm card punch and to get bacterium dish, with the sterilization transfer needle bacterium dish is reversed and be transplanted on the flat board, placed under 25 ℃ of conditions constant temperature culture 7 days.
1.3.3 result's inspection
Measure bacterium colony expansion diameter with ruler intersection mensuration, expand diameter with not dispenser contrast bacterium dish and compare, obtain medicament and suppress growth rate.
1.3.4 statistical analysis
Do regression analysis according to each drug concentration logarithm value and corresponding mycelial growth inhibition rate probability value, calculate each medicament virulence regression equation, correlation coefficient (r) and EC
50
When carrying out the medicament joint toxicity measuring,, estimate the synergy type of complex composition according to the coefficient of synergism (SR) of Wadley method calculating complex composition.Estimate the synergistic effect of composite reagent according to coefficient of synergism (SR), promptly SR＜0.5 is an antagonism, and 0.5≤SR≤1.5 are summation action, and SR＞1.5 are synergistic effect.Coefficient of synergism (SR) calculates by formula (1), (2):
In the formula:
X
1----complex composition EC
50Theoretical value, unit is mg/L;
P
A----percentage composition of A in the complex composition, unit is percentage (%);
P
B----percentage composition of B in the complex composition, unit is percentage (%);
A----complex composition EC
50Theoretical value, unit is mg/L;
B----complex composition EC
50Theoretical value, unit is mg/L.
In the formula:
The coefficient of synergism of SR----complex composition;
X
1----complex composition EC
50Theoretical value, unit is mg/L;
X
2----complex composition EC
50Measured value, unit are mg/L.
1.4 experimental result
Through indoor virulence experiment, can draw Osthole with carbendazim weight ratio 1: 10～20 has summation action, in 1: 25～40 scopes synergistic effect is arranged all, 1: 30～40 is preferred compound proportion, be optimum compound proportion routine at 1: 35.
Table 3 Osthole and the screening of carbendazim optimum compound proportion example
| Medicament | Virulence regression equation | Correlation coefficient r | EC 50Measured value (mg/L) | EC 50Theoretical value (mg/L) | Coefficient of synergism (SR) |
| 94.7% Osthole (A) | y＝2.18+1.50x | 0.9614 | 75.3819 | -- | -- |
| 98% carbendazim (B) | y＝5.65+1.29x | 0.9989 | 0.3128 | -- | -- |
| A∶B＝1∶10 | y＝6.56+2.73x | 0.9791 | 0.2668 | 0.3439 | 1.29 |
| A∶B＝1∶15 | y＝6.57+2.55x | 0.9860 | 0.2430 | 0.3336 | 1.37 |
| A∶B＝1∶20 | y＝6.11+1.77x | 0.9860 | 0.2367 | 0.3284 | 1.39 |
| A∶B＝1∶25 | y＝6.50+2.18x | 0.9952 | 0.2065 | 0.3253 | 1.58 |
| A∶B＝1∶30 | y＝6.89+2.56x | 0.9903 | 0.1451 | 0.3232 | 2.23 |
| A∶B＝1∶35 | y＝6.81+1.85x | 0.9966 | 0.1059 | 0.3217 | 3.34 |
| A∶B＝1∶40 | y＝6.37+1.57x | 0.9817 | 0.1344 | 0.3206 | 2.39 |
Embodiment 2
Field plot trial
Filter out Osthole according to indoor compound proportion screening experiment: carbendazim=be best proportioning at 1: 35, during Osthole 1mg, carbendazim is 35mg.We have processed 36% Osthole carbendazim suspending agent.
2.1 arrange test medicine and sub-district
" " pesticide field efficacy medicine test criterion (two) " the 118th part: bactericidal agent for preventing and treating ring rot of apple " according to the chief editor of The Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC designs the field control effectiveness test of 36% Osthole carbendazim suspending agent to ring rot of apple.
2.1.1 supply the reagent agent
200 times of 1% osthole water-emulsion agent.(academy of agricultural sciences, Jiangsu Province provides)
500 times of 50% carbendazim suspending agents.(granary, Jiangsu insecticide factory provides)
400,500,600 times of 36% Osthole carbendazim suspending agents.(academy of agricultural sciences, Yantai City makes)
2.1.2 contrast medicament
2000 times of 10% generation high-moisture shot agent.(just reaching (China) investment company earlier provides)
2.1.3 sub-district design
6 processing are established in test altogether, and 24 sub-districts are established in 4 repetitions of every processing altogether, and 4 of every sub-districts become the strain tree.Each sub-district randomized arrangement.Spraying equipment adopts workers and peasants' 26 type high pressure power driven sprayers, with in the tree crown all the branch blades even medicine, till dripping.
2.2 control time and drug effect computational methods
2.2.1 control time and method
Picking time, centre 2 strains were chosen in every sub-district, investigated whole fruits, and the total fruit of record number, sick fruit number calculate preventive effect with diseased fruit rate; Storage period, after respectively handling fruit after will gathering and rejecting disease, worm, wound fruit, the same day, the dress basket was put under the natural room temperature, store back 10 days, 20 days, the 30 days once sick fruit numbers of investigation respectively, with accumulation diseased fruit rate calculating preventive effect.
2.2.2 drug effect computational methods
2.3 result of the test
The 36% Osthole carbendazim suspending agent of on the basis of lab screening, processing is had outstanding performance in field trial; 400 times, the 500 times preventive effects in picking time are all more than 85%; The preventive effect of storage period all is significantly higher than the preventive effect of 500 times of 200 times of 1% Ostholes and 50% carbendazim all more than 82%.
Table 4 36% Osthole carbendazim is prevented and treated the ring rot of apple field efficacy
Embodiment 3
Safety testing
In the complex composition carbendazim content than the minimizing of commercially available prod 15 percentage points, so it is satisfactory to people and animals' toxicity with safety to crop; Osthole content is 1% in the complex composition, through assert that the toxicity test result of mechanism shows that 1% osthole water-emulsion agent belongs to the micro-virus kind agricultural chemicals:
Rat acute per os toxicity
♀＞5000mg/kg;
Rat acute percutaneous toxicity
♀＞5000mg/kg;
It is 0 (1h) that lagophthalmos stimulates integration, is 0 (1: 100) dilution behind the 48h, to the eye nonirritant;
It is 0 (4h) that lagophthalmos stimulates integration, to the skin nonirritant.
Claims (5)
1. the complex composition of Osthole and carbendazim is characterized in that containing Osthole and carbendazim, and both weight ratios are 1: 25～40.
2. according to the complex composition of described Osthole of claim 1 and carbendazim, the weight ratio that it is characterized in that Osthole and carbendazim is 1: 30～40.
3. according to the complex composition of described Osthole of claim 1 and carbendazim, the weight ratio that it is characterized in that Osthole and carbendazim is 1: 35.
4. the complex composition of claim 1 or 2 or 3 described Ostholes and carbendazim is applied to prevent and treat ring rot of apple.
5. the complex composition according to said Osthole of claim 3 and carbendazim is applied to prevent and treat ring rot of apple, it is characterized in that the 36% Osthole carbendazim suspending agent field dilution 400-500 that processes doubly prevents and treats ring rot of apple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102498764A CN101455203B (en) | 2008-12-28 | 2008-12-28 | Compound composition of osthole and carbendazim and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102498764A CN101455203B (en) | 2008-12-28 | 2008-12-28 | Compound composition of osthole and carbendazim and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101455203A CN101455203A (en) | 2009-06-17 |
| CN101455203B true CN101455203B (en) | 2012-05-02 |
Family
ID=40766523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102498764A Expired - Fee Related CN101455203B (en) | 2008-12-28 | 2008-12-28 | Compound composition of osthole and carbendazim and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101455203B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108378041A (en) * | 2018-02-10 | 2018-08-10 | 山东省烟台市农业科学研究院 | A kind of biogas slurry and bactericide compounded composition and the application in preventing Apple disease |
| CN114903060A (en) * | 2022-05-31 | 2022-08-16 | 长江师范学院 | A kind of Chinese herbal medicine and compound pesticide for preventing and treating black spot disease of stem nodule |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3577110B2 (en) * | 1994-08-29 | 2004-10-13 | 株式会社ヤクルト本社 | ACAT inhibitor |
| CN1586198A (en) * | 2004-07-06 | 2005-03-02 | 江苏省农业科学院 | Crop powdery mildew control by osthole and its compound composition |
| CN101036461A (en) * | 2007-04-26 | 2007-09-19 | 江苏省农业科学院 | Eugenol, osthol complex biological bactericide |
-
2008
- 2008-12-28 CN CN2008102498764A patent/CN101455203B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3577110B2 (en) * | 1994-08-29 | 2004-10-13 | 株式会社ヤクルト本社 | ACAT inhibitor |
| CN1586198A (en) * | 2004-07-06 | 2005-03-02 | 江苏省农业科学院 | Crop powdery mildew control by osthole and its compound composition |
| CN101036461A (en) * | 2007-04-26 | 2007-09-19 | 江苏省农业科学院 | Eugenol, osthol complex biological bactericide |
Non-Patent Citations (2)
| Title |
|---|
| Toshihiro Okamoto,et al..Synthetic Derivatives of Osthole for the Prevention of Hepatitis.《Medicinal Chemistry》.2007,第3卷(第1期),第35-44页. * |
| 王春梅,等.蛇床子素防治黄瓜白粉病研究.《江苏农业科学》.2005,(第4期),第57-58页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101455203A (en) | 2009-06-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101955393A (en) | Process for preparing bamboo vinegar liquid fertilizer | |
| CN104322572B (en) | A kind of plant insecticide | |
| CN101589716B (en) | Osthole and matrine compound pesticide and application thereof | |
| CN105284864A (en) | Forest fruit trunk-injection insecticide and preparation method and application thereof | |
| CN101717303B (en) | Biological organic water-soluble fertilizer and preparation method thereof | |
| Kumar et al. | Eco-friendly management of Alternaria leaf spot of brinjal (Solanum melongena L.) | |
| CN105284917B (en) | A kind of plant pesticide composition containing nimbin and caryophyllus oil | |
| CN101455203B (en) | Compound composition of osthole and carbendazim and application thereof | |
| CN104286031B (en) | A kind of biological combination sterilization composition and application thereof | |
| CN103891754B (en) | A kind of composition pesticide of clothianidin-containing and fluazinam and its production and use | |
| CN102388873A (en) | Downy mildew fungicide for promoting crop growth and application thereof | |
| CN101496518B (en) | Eugenol and myclobutanil compositional bactericidal agent for preventing and treating anthracnose of crop | |
| CN103918657A (en) | Fungicidal composite for preventing and controlling apple diseases and application of fungicidal composite | |
| CN103478129B (en) | Agricultural compound bactericide and application thereof | |
| CN102643145A (en) | Active biological medicine fertilizer specially used for Dangshan pear | |
| CN107372556A (en) | A kind of suspension seed-coating agent containing pyraclostrobin, amino-oligosaccharide and Diacloden | |
| CN106689140A (en) | Pyraziflumid-containing bactericidal composition and application thereof | |
| CN102726394A (en) | Sterilization composition containing propamidine and chitosan oligosaccharides | |
| CN103548849B (en) | Synergistic compound biological bactericide and application thereof | |
| CN103875670B (en) | Compound composition of eugenol and osthole and application thereof | |
| CN103918659A (en) | Pesticide composite including tolfenpyrad and fluazinam and preparation method and application of pesticide composite | |
| CN103875675B (en) | The complex composition of Osthole and imazalil and application thereof | |
| CN103858885A (en) | Bactericidal composition for controlling apple diseases and application thereof | |
| CN104798794A (en) | Captan and imazalil compound composition and application thereof | |
| CN103651395B (en) | Sterilization composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120502 Termination date: 20171228 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |