CN111903697A - a bactericidal composition - Google Patents

Abstract

The invention provides a bactericidal composition. The bactericidal composition comprises active compounds tetrazolium and cyazofamid, and the weight ratio of tetrazolium and cyazofamid is 10:1-1:10. The invention also provides the use of the bactericidal composition in preventing and treating plant pathogenic bacteria such as Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Soft Rot and the like. The invention also relates to a method for controlling plants, plant parts, plant propagation material and subsequently grown plant organs from Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Soft-Rot fungi and bacteria Methods.

Description

a bactericidal composition

technical field

The present invention relates to a bactericidal composition, in particular to a bactericidal composition for the protection of plants or seeds from the attack of Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium and Soft Rot. method.

Background technique

With regard to pesticide activity, especially for crop protection, one of the core issues of research carried out in this technical field is the improvement of properties, especially in terms of biological activity and the maintenance of this activity over a certain period of time.

Tetrazopyridine, test code NF-171; CAS: 500207-04-5; chemical formula is [6-[[[[(z)-(1-methyl-1H-tetrazol-5-yl) (benzene base) methylene] amino] oxy] methyl] pyridin-2-yl] tert-butyl carbamate; molecular structural formula is:

Tetrazopyramide is an oxime ether fungicide developed by Japan's Caoda Company, which has a good control effect on downy mildew and blight. Tetrazopyridine is known from CN02817805.X.

Due to the current environmental and economic requirements for fungicides, such as requirements for activity spectrum, toxicity, selectivity, application rate, residue composition and favorable production feasibility, and also due to the possible presence of, for example, drug resistance The problem, therefore, is the ongoing task of developing new fungicides that are in some ways superior to existing fungicides.

SUMMARY OF THE INVENTION

It is an object of the present invention to provide a fungicidal composition which is effective in reducing the activity in terms of reducing the application rate and improving the activity spectrum of the known compounds tetrazolium and cyazofamid at a reduced total amount of active compound applied. Compounds have improved activity (synergistic) against phytopathogenic bacteria at the total amount of compound applied.

We have found that simultaneous, i.e. combined or separate application of tetrazopyramid and cyfamid, or sequential application of tetrazopyramid and cyfamid, provides better control of harmful phytopathogens than application of the individual compounds alone; in particular It is an unexpected synergistic effect on the prevention and control of plant pathogenic bacteria such as Pythium, Fusarium, Alternaria, Black Star, Clubroot, and Soft Rot.

The invention provides a bactericidal composition. The composition is binary compounded by tetrazolium and cyazofamid, so that the obtained composition has a gain effect in the control effect, and expands the bactericidal spectrum. The multi-purpose effect of one drug can effectively slow down or prevent bacteria from developing drug resistance. Surprisingly, the fungicidal activity of the fungicidal compositions of the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, real synergistic effect, not just a supplement of activity.

The synergistic effect is particularly pronounced when the active compounds are present in the germicidal compositions of the present invention in specific weight ratios. However, the weight ratio of the active compounds in the germicidal compositions of the present invention may vary within a certain range.

A kind of bactericidal composition of the present invention adopts the following technical scheme to realize:

A bactericidal composition, characterized in that it contains active ingredients tetrazolium and cyfamid, wherein the weight percentages of tetrazolium and cyfamid are 10:1-1:10, preferably 5:1 -1:5, more preferably 3:1-1:3, more preferably 1:1.

The weight ratio of tetrazolium and cyazofamid in the present invention can also be, for example, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3 :1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10.

Said bactericidal composition further comprises filler and/or surfactant.

A method of controlling phytopathogenic bacteria by applying a fungicidal composition to the pathogenic bacteria and/or their environment, or to plants, plant parts, seeds, soil, areas, materials or spaces.

A method for preventing and controlling phytopathogenic bacteria, wherein tetrazolium and cyazofamid are applied simultaneously, or separately, or successively.

A germicidal composition comprising tetrazolium and cyazofamid together with fillers and/or surfactants.

A bactericidal composition can be formulated into any agriculturally acceptable dosage form. The dosage forms of the bactericidal composition are suspending agent, seed coating agent, suspoemulsion, wettable powder, water dispersible granule, microcapsule suspending agent, coated granule, extruded granule, emulsifiable concentrate, microemulsion, water emulsion , Effervescent tablets, ultra-low volume liquid.

Use of the bactericidal composition for controlling fungi and bacteria on cereals, fruits and vegetables.

The bactericidal composition is used for preventing and treating Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Soft Rot fungi and bacteria on cereals, fruits and vegetables.

The bactericidal composition is used for protecting plant propagation material and subsequently growing plant organs against attack by Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Softrot fungi and bacteria.

Use of the fungicidal composition to control soil pathogenic or saprophytic fungi and bacteria at the site of desired control.

The bactericidal composition is used in various crop plants such as rapeseed, tomato, Chinese cabbage, banana, cotton, vegetable varieties (such as cucumbers, beans, tomatoes and cucurbits), barley, grasses, oats, coffee, potatoes, It is especially important to control a large number of fungi in corn, apples, pears, rice, rye, soybeans, grapevines, wheat, ornamental plants, sugarcane, and many seeds.

A method for controlling plants, plant parts, plant propagation material and subsequently grown plant organs from Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Soft Rot fungi and bacterial phytopathogens A method comprising applying the fungicidal composition of the present invention to a seed treatment, foliar application, stem application, saturating, dripping, pouring, spraying, spraying, dusting, spreading or spreading the fungicidal composition of the present invention at an agronomically effective and substantially non-phytotoxic application rate Methods such as smoke are applied to plants, plant parts, plant propagation material, or the soil or cultivation medium in which the plants are growing or need to be grown.

The present invention provides a bactericidal composition which can have higher activity and maintain the activity for a longer time. The bactericidal composition has a lower dosage, and at the same time, the toxicity of the bactericidal composition is also low, and is suitable for plants with fungal diseases such as Pythium, Fusarium, Alternaria, Black Star, Plasmorhizium, Soft Rot Fungus, Seed and soil for control.

Detailed ways

The bactericidal composition of the present invention has strong activity against various phytopathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by phytopathogenic bacteria. The bactericidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as Plasmodiomycetes, Oomycetes, Chytridiomycetes, Hypomycetes, Ascomycetes, Basidiomycetes, Deuteromycetes, and the like.

The bactericidal composition of the present invention also has very good bactericidal properties and can be used to control phytopathogenic bacteria. Such as Pseudomonas, Rhizobacteria, Enterobacteriaceae, Corynebacterium and Streptomyces.

The fungicidal composition of the present invention can be used as a foliar fungicide in crop protection, as a fungicide for seed dressing and as a soil fungicide.

The bactericidal composition of the present invention is especially suitable for controlling the following phytopathogenic bacteria:

Podosphaeradiseases, eg caused by Podosphaeraleucotricha;

Sphaerothecadiseases, eg caused by Sphaerothecafuliginea;

Uncinuladiseases, for example caused by grape husks (Uncinulanecator);

Rust diseases, for example: Gymnosporangium diseases, for example caused by Gymnosporangium sabinae;

Hemileiadiseases, for example caused by Hemileiavastatrix;

Phakopsoradiseases, for example caused by Phakopsorameibomiae;

Pucciniadiseases, for example caused by Puccinia recondite, Pucciniagraminis or Puccinia striiformis;

Uromyces diseases, for example caused by Uromyces appendiculatus;

Oomycetediseases, such as:

Albugodiseases, for example caused by Albugocandida;

Bremiadiseases, eg caused by Bremialactucae;

powderymildew, for example: Blumeriadiseases, for example caused by Blumeriagraminis;

Downy mildews (Peronosporadiseases), for example caused by Peronosporapisi and Peronosporabrassicae;

Phytophthora infestans, for example caused by Phytophthorainfestans;

Plasmoparadiseases, eg caused by Plasmoparaviticola;

Pseudoperonosporadiseases, eg caused by Pseudoperonosporahumuli or Pseudoperonosporacubensis;

Pythium diseases, for example caused by Pythium ultimum;

Alternaria diseases, eg caused by Alternaria solani;

Cercosporadiseases, for example caused by Cercosporabeticola;

Cladiosporum diseases, eg caused by Cladiosporium cucumerinum;

Cochliobolusdiseases, eg caused by Cochliobolussativus (Conidiaform: Drechslera, Syn: Helminthosporium) or Cochliobolusmiyabeanus;

Colletotrichumdiseases, eg caused by Colletotrichumlindemuthanium;

Cycloconium diseases, for example caused by Cycloconium oleaginum;

Diaporthediseases, eg caused by Diaportthecirri;

Elsinoediseases, eg caused by Elsinoefawcettii;

Gloeosporium diseases, eg caused by Gloeosporium laeticolor;

Glomerelladiseases, eg caused by Glomerellacingulata;

Guignardiadiseases, eg caused by Guignardiabidwelli;

Leptosphaeria diseases, for example caused by Leptosphaeria maculans; Leptosphaerianodorum;

Magnaporthediseases, eg caused by the fungus Magnaporthegrisea;

Mycosphaerelladiseases, eg caused by Mycosphaerellagraminicola; Mycosphaerellaarachidicola; Mycosphaerellafijiensisi;

Phaeosphaeria diseases, eg caused by Phaeosphaerianodorum;

Pyrenophoradiseases, eg caused by Pyrenophorateres or Pyrenophoratriticirepentis;

Rhynchosporium diseases, eg caused by Rhynchosporium secalis;

Septoriadiseases, eg caused by Septoriaapii in celery and Septorialycopercisi in tomato;

Typhuladiseases, for example caused by Typhulaincarnata;

Venturiadiseases, for example caused by Venturiainaequalis;

Root, sheath and stem diseases such as:

Corticium diseases, eg caused by Corticium graminearum;

Fusarium diseases, for example caused by Fusarium oxysporum;

Sturgeon diseases (Gaeumannomyces diseases), for example caused by Gaeumannomyces graminis;

Rhizoctonia diseases, eg caused by Rhizoctonia solani;

Sarocladiumdiseases, eg caused by Sarocladiumoryzae;

Sclerotium diseases, eg caused by Sclerotiumoryzae;

Thielaviopsis diseases, eg caused by Thielaviopsis basica;

Ear and panicle diseases, including corn on the cob, such as:

Alternaria diseases, eg caused by Alternaria spp.;

Aspergillus diseases, eg caused by Aspergillus flavus;

Cladosporium diseases, eg caused by Cladosporium cladosporioides;

Clavicepsdiseases, e.g. caused by Claviceps purpurea;

Fusarium diseases, for example caused by Fusarium culmorum;

Gibberella diseases, eg caused by Gibberellazeae;

rice disease (Monographella), for example caused by Monographellanivalis;

Smut and smut, for example: Sphacelothecadiseases, for example caused by Sphacelothecareiliana;

Tilletiadiseases, eg caused by Tilletiacaries;

Urocystisdiseases, for example caused by Urocystisocculta;

Ustilagodiseases, for example caused by Ustilagonuda;

Fruit rot and fungal diseases such as:

Aspergillus diseases, eg caused by Aspergillus flavus;

Botrytis diseases, eg caused by Botrytis cinerea;

Penicillium diseases, eg caused by Penicillium expansum and Penicillium purpurogenum;

Sclerotiniadiseases, eg caused by Sclerotiniasclerotiorum;

Verticilium diseases, eg caused by Verticilium al boatrum;

Seed and soil-borne rot, mold, blight, rot and damping off:

Alternaria diseases, eg caused by Alternaria brassicicola;

Aphanomyces diseases, eg caused by Aphanomyceseuteiches;

Ascochytadiseases, eg caused by Ascochytalentis;

Aspergillus diseases, eg caused by Aspergillus flavus;

Cladosporium diseases, eg caused by Cladosporium herbarum;

Cochliobolusdiseases, eg caused by Cochliobolussativus (Conidiaform: Drechslera, BipolarisSyn: Helminthosporium);

Colletotrichumdiseases, eg caused by Colletotrichumcoccodes;

Fusarium diseases, for example caused by Fusarium culmorum;

Gibberella diseases, for example caused by Gibberellazeae;

Macrophominadiseases, eg caused by Macrophomina phaseolina;

Microdocium disease, for example caused by Microdochiumnivale;

Monographelladiseases, eg caused by Monographellanivalis;

Penicillium diseases, eg caused by Penicillium expansum;

Phomadiseases, for example caused by Phomalingam;

Erwinia disease (Erutima), such as vegetable soft rot caused by E. carafavoratear, saro Eoriora;

Phomopsis diseases, for example caused by Phomopsissojae;

Phytophthoradiseases, for example caused by Phytophthoracactorum;

Pyrenophoradiseases, eg caused by Pyrenophoragraminea;

Pyriculariadiseases, eg caused by Pyriculariaoryzae;

Pythium diseases, for example caused by Pythium ultimum;

Rhizoctonia diseases, eg caused by Rhizoctonia solani;

Rhizopus diseases (Rhizopusdiseases), such as by rice seedling blight (Rhizopusoryzae, Rhizopuschinesnsis, Rhizopus Javanicus, Rhizopusarrhizus), sweet potato root rot (Rhizopusnodosus, Rhizopusoryzae), pepper stem rot (Rhizopusstolonifer), melon black root mold (Rhizopusnodosus, Rhizopusoryzae) Rhizopusstolonifer), strawberry soft rot (Rhizopusnigricans), lily soft rot (Rhizopusoryzae), etc.;

Sclerotium diseases, eg caused by Selerotium rolfsii;

Septoriadiseases, eg caused by Septorianodorum;

Typhuladiseases, for example caused by Typhulaincarnata;

Verticillium diseases, eg caused by Verticillium dahliae;

Canker, broom and dieback, such as:

Nectriadiseases, eg caused by Nectriagalligena;

Fusarium wilt, such as:

Moniliniadiseases, e.g. caused by the drupe Monilinialaxa;

Leaf blisters or leaf shrinkage, including deformities of flowers and fruits, such as:

Exobasidium diseases, for example caused by damaging Exobasidium vexans;

Taphrinadiseases, for example caused by Taphrinadeformans;

Diseases of flowers and seeds, such as:

Botrytis diseases, eg caused by Botrytis cinerea;

Root and shoot diseases, such as:

Rhizoctonia diseases, eg caused by Rhizoctonia solani;

Helminthosporium diseases, for example caused by Helminthosporium solani;

Clubroot, eg Plasmodiophoradiseases, eg caused by Plasmodiophorabrassicae.

The fungicidal composition of the present invention is particularly effective against seed diseases or diseases in early stages of growth of various plants caused by the following: Aspergillus spp., Penicillium spp., Fusarium ( Fusariumspp.), Gibberellaspp., Tricodermaspp., Thielaviopsisspp., Rhizopusspp., Mucorspp., Corticiumspp.), Rhomaspp., Rhizoctoniaspp., Diplodiaspp..

The fungicidal compositions of the present invention can also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soil or cultivation media. Examples of soil-borne fungal pathogens include Alternariaspp., Ascochytaspp., Botrytiscinerea, Cercosporaspp., Claviceps purpurea, Claviceps purpurea Fungi (Cochliobolussativus), Colletotrichumspp., Epicoccus (Epicoccumspp., Fusarium graminearum), Fusarium moniliforme, Fusarium oxysporum, Fusarium proliferatum , Fusarium solani, Fusarium subglitinans, Helminthosporiumspp, Microdochiumnivale, Pencilliumspp, Phomaspp., Pyrenophoragraminea, Pyriculariaoryzae, Rhizoctoniasolani, Rhizoctonia cerealis, Sclerotiniaspp. Septoriaspp.), Sphacelothecareilliana, Tilletiaspp., Typhulaincarnate, Urocystisocculta, Ustilagospp. or Verticillium spp..

Soil pathogens include blight, Fusarium, Phytophthora, damping off, root rot, Pythium, Botrytis, and soft rot. Under normal circumstances, soil pathogens can produce a large number of bacteria. As long as the conditions are favorable for the growth and development of the bacteria and the host is infected, the bacteria can multiply and infect the host. In the presence of the infected host, these bacteria can Entering the continuous pathogenic stage, with the continuous cropping of crops, the bacteria multiply and spread, but after the nutrients are consumed or the soil conditions such as temperature and humidity are unfavorable for the bacteria, the bacteria can enter the dormant period. In the absence of susceptible hosts, soil-borne pathogens can also survive in the soil. In addition to soil pathogens having a wide range of hosts, they can also survive on non-host root surfaces or stumps and leaves. Their ability to compete with saprophytes is inseparable. But different germs are different, like Fusarium can survive almost indefinitely in the soil.

The crops treated by the fungicidal compositions of the present invention are, for example, but not limited to, cereals, vegetables, alfalfa, soybeans, turf, wood, trees, fruit trees or horticultural plants.

The crops mainly include field crops such as corn, soybean, cotton, canola oilseeds, such as Brassicanapus (eg canola), turnip (Brassicarapa), mustard (B. juncea) ( such as mustard (mustard) and Ethiopian mustard (Brassica carinata), rice, wheat, sugar beet, sugar cane, oats, brown wheat, barley, millet, triticale, flax, vines and fruit or vegetable crops of various plant classes such as roses Families (Rosaceae sp.) (for example, pome fruits such as apples and pears, and stone fruits such as apricots, cherries, almonds and peaches, berries such as strawberries), Ribesioidae sp., Juglandaceae sp. ), Betulaceae (Betulaceaesp.), Anacardiaceae (Anacardiaceaesp.), Beech (Fagaceaesp.), Moraceae (Moraceaesp.), Oleaceae (Oleaceaesp.), Actinidaceae (Actinidaceaesp.), Lauraceae (Lauraceaesp.) , Musaceae sp. (e.g. banana trees and plantings), Rubiaceae sp. (e.g. coffee), Theaceae sp., Sterculiceae sp., Rutaceae sp. (eg lemons, oranges, and grapefruits); Solanaceae sp. (eg, tomatoes, potatoes, peppers, eggplants), Liliaceae (Liliaceae sp.), Compostiae sp. (eg lettuce, artichokes, and chicory- Including root chicory, endive or common chicory), Umbelliferae sp. (e.g. carrot, parsley, celeriac and celeriac), Cucurbitaceae sp. (e.g. cucumber) -Including pickling cucumber, squash, watermelon, gourd and melon), Alliaceae (e.g. onion and leek), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, Cauliflower, Brussels sprouts, greens, cabbage, radishes, horseradish, solitaire, Chinese cabbage), Leguminosaesp. (eg fodder beet, spinachbeet, spinach, beetroot), Malvaceae (eg okra), Asparagaceae (A sparagaceae) (eg, asparagus); horticultural and forest crops; ornamental plants; and genetically modified homologs of these crops.

The bactericidal composition of the invention is especially suitable for controlling apple scab, pear scab, beet brown spot, cruciferous vegetable clubroot, vegetable soft rot, tomato root rot, corn stalk base rot, rice root rot Blight, tomato early blight, etc.

The fungicidal compositions of the present invention can be used for the protection of plants, plant parts, plant propagation material and subsequently grown plant organs against attack by phytopathogenic fungi; in particular against Pythium, Fusarium, Alternaria, Black Uses for Astral, Plasmorhizium, Soft-Rot Fungal and Bacterial Infestations.

Another object of the present invention is to provide a method for controlling plants, plant parts, plant propagation material and subsequently grown plant organs from phytopathogenic bacteria, in particular from Pythium, Fusarium, Alternaria, Black Star, A method of infestation with Phytophthora, soft-rot fungi and bacteria, comprising applying the fungicidal composition of the present invention at an agronomically effective and substantially non-phytotoxic application rate to seed treatment, foliar application, stem application, drenching, dripping , pouring, spraying, spraying, dusting, spreading or smoking to plants, plant parts, plant propagation material or the soil or cultivation medium in which the plant is growing or needs to be grown.

The fungicidal composition of the present invention can treat all plants and plant parts. "Plants" refers to all plants and plant populations, such as desirable and undesirable wild plants, cultivated plants and plant varieties (whether or not protected by plant varieties or plant breeders' rights). Cultivated plants and plant varieties may be those obtained by conventional propagation and breeding methods, which may be supplemented or supplemented by one or more biotechnological methods, such as the use of double haploids, protoplast fusion, random and directed mutagenesis, molecular Or genetic markers, or using bioengineering and genetic engineering methods. Plant parts refer to all aerial and underground parts and organs of plants, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruiting bodies, fruits and seeds as well as roots, bulbs and rhizomes. Crops as well as vegetative and generative propagation material such as cuttings, bulbs, rhizomes, runners and seeds also belong to plant parts.

The term "plant propagation material" is understood to mean all reproductively capable plant parts, such as seeds, which can be used to propagate the latter, as well as plant material such as cuttings or tubers (eg potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated and effective plants to be inhibited post-emergence or post-emergence from the soil. Young plants can be protected by full or partial treatment by dipping before transplanting.

Plant parts and subsequently grown plant organs are any part of a plant that is produced from plant propagation material such as seeds. Plant parts, plant organs and plants can also benefit from protection from pathogen damage obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs grown after certain loci can also be regarded as plant propagation material, which themselves can be applied (or treated) with a fungicidal composition; thus the resulting Plants, other plant parts and other plant organs may also benefit from application of the fungicidal composition.

The preferred propagation material of the present invention is seed. The method of seed treatment includes, for example, a method of diluting a liquid or solid chemical, or directly immersing the seed in a liquid state solution without diluting, and immersing the chemical in the seed, mixing a solid chemical or a liquid chemical with the seed, and encapsulating the seed. Methods of coating the surface of seeds with chemicals, spraying them near the seeds at the same time of planting, etc.

The cultivation medium described in the present invention refers to a support capable of rooting and growing crops, such as soil, water, etc. Specific raw materials can be, for example, sand, pumice, vermiculite, diatomaceous earth, agar, and gelatinous materials. , polymer substances, asbestos, sawdust, bark, etc. The preferred growing medium is soil.

Methods of applying chemicals to soil, for example, diluting liquid chemicals in water or directly applying them without dilution to the roots of plants or seedling fields for raising seedlings. The method of spraying powder, water-dispersible granules, etc. into the soil before sowing and mixing with the soil as a whole. Before sowing or before planting plants, the powder and water-dispersible granules are diluted and sprayed in the planting holes and sowing trenches. method of sowing, etc.

The present invention provides a method for preventing and treating plant pathogenic bacteria, in particular, preventing and controlling plant pathogenic bacteria such as Pythium, Fusarium, Alternaria, Black Star Fungus, Plasma rhizogenes, and soft rot fungi. eradicated.

The tetrazopyramide of the present invention is administered in combination/combination with cyazofamid. This includes separate, sequential or simultaneous administration of tetrazolium and cyazofamid. Preferably, the combination of tetrazolium and cyfamid is in the form of a composition comprising tetrazolium and cyfamid.

The composition of the present invention can be mainly in the form of a preparation, that is, the various substances in the composition have been mixed, and the components of the composition can also be provided in the form of a single dose, which is mixed in a bucket or tank before use, and then diluted to a desired concentration. Among them, the preparation form provided by the present invention is preferred.

As a further improvement of the present invention, the fungicidal composition of the present invention can be formulated into any agriculturally acceptable dosage form.

As a further improvement of the present invention, the dosage forms of the bactericidal composition of the present invention are suspending agent, seed coating agent, wettable powder, water dispersible granule, microcapsule suspending agent, coated granule, extruded granule, emulsifiable concentrate, microgranular Emulsion, water emulsion, effervescent tablet, ultra-low volume liquid, suspoemulsion.

The bactericidal composition of the present invention comprises tetrazolium and cyazofamid, fillers and/or surfactants.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject, such as a plant, crop or grass. Therefore, the filler is preferably inert, and at least should be agriculturally acceptable. The filler can be solid or liquid.

The inactive vehicle that can be used in the present invention can be either solid or liquid, and can be used as a solid vehicle material, such as: plant powders (such as soybean meal, starch, grain powder, wood powder, bark powder, Sawdust, walnut shell powder, bran, cellulose powder, coconut shell, particles of corn cobs and tobacco stems, residues after extraction of plant essence, etc.), paper, sawdust, synthetic polymers such as crushed synthetic resins, clays ( Such as kaolin, bentonite, acid china clay, etc.), talc. Silicas (such as diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (pumice, attapulgite and zeolite, etc.), fired diatomaceous earth, sand, plastic media, etc. (such as polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, and soil fertilizers, These substances may be used alone or in combination of two or more.

The materials that can be used as liquid vehicles can be selected from the following materials, such as water, alcohols (such as methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (such as acetone, methyl ethyl ketone, Diisobutyl ketone, cyclohexanone, etc.), ethers (such as diethyl ether, dioxane, methylcellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (such as kerosene, mineral oil, etc.), aromatic hydrocarbons (such as benzene, toluene, xylene, mineral spirits, alkyl naphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, Mineral and vegetable oils, animal oils, etc.

To emulsify, disperse, and/or wet the active ingredient compound, surfactants can be used, for example, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl aryl ether, polyoxyethylene higher fatty acid ester, polyoxyethylene Phosphate esters of alcohols or phenols, fatty acid esters of polyhydric alcohols, alkyl aromatic sulfonic acids, naphthalene sulfonic acid polymers, lignosulfonic acid salts, polymer comb-shaped branched copolymers, butyl naphthalene sulfonic acid salts, alkyl sulfonates Aryl sulfonates, sodium alkyl sulfosuccinates, oils and fats, condensates of fatty alcohols and ethylene oxide, polyacrylates such as alkyl taurates, and protein hydrolyzates. Suitable oligosaccharides or polymers are for example based on vinyl monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with eg (poly)ols or (poly)amines.

For dispersion stabilization, attachment and/or bonding of the active ingredient compounds, for example, xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate, and natural phospholipids (such as brain) can be used. phospholipids and lecithin) and auxiliary agents such as synthetic phospholipids, bentonite, and sodium lignosulfonate.

Among them, the antifreeze can be selected from ethylene glycol, propylene glycol, glycerol and sorbitol. Adjuvants such as naphthalene sulfonic acid polymers, polymeric phosphates and the like can be used as deflocculants for suspending products.

As the defoaming agent, a silicone defoaming agent can be used.

Colorants that can be used, such as inorganic pigments such as iron oxide, titanium oxide and Prussian blue; and organic pigments/dyes: alizarin dyes, azo dyes and metal phthalocyanine dyes; and trace elements such as iron salts, manganese salts, Boron, copper, cobalt, molybdenum and zinc salts.

Optionally, other additional components such as protective colloids, binders, thickeners, thixotropic agents, penetrants, stabilizers, masking agents may also be included.

The formulations according to the invention can be prepared by mixing the active compounds with conventional additives in a known manner. Said conventional additives such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents and, if desired, driers and colorants , stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

These compositions include not only concentrated commercial compositions that can be applied immediately to the subject to be treated by means of suitable equipment, such as spraying or dusting equipment, but also to be diluted prior to application to the subject.

The tetrazopyramid- and cyfamid-containing compounds of the present invention can also be administered in combination with other active ingredients, for example to broaden the spectrum of activity or prevent the development of resistance. Such other active ingredients are, for example, fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.

Biological test case

A synergistic effect exists when the effect of the active compound combination exceeds the sum of the effects when the individual active compounds are administered individually. The expected effect of a particular combination of two active compounds can be calculated using the so-called "Colby formula" (see S.R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22) as follows: If

X is the activity when active compound A is used in mg/ha or mppm concentration;

Y is the activity when active compound B is used at ng/ha or nppm concentration, expressed as a percentage of the untreated control;

E is the activity when active compounds A and B are used in m and ng/ha or m and nppm concentrations,

So

If the actual observed activity (O) is greater than the expected activity (E), then the composition is synergistic.

The following biological test examples are used to illustrate the present invention. However, the present invention is not limited to these Examples.

Test 1: Alternaria test (tomato)

Solvent: 24.5 parts by weight of acetone

24.5 parts by weight of dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

In order to obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a certain amount of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test for protective activity, young tomato plants are sprayed with the active compound preparation at certain application rates. After the sprayed coating has dried, the plants are inoculated with an aqueous spore suspension of Alternaria. The plants are then placed in an incubator at about 20°C and 100% relative atmospheric humidity.

Test results were evaluated 3 days after incubation. 0% means efficacy equivalent to the control, and 100% efficacy means no symptoms.

Table 1

Table 1 clearly shows that the actual efficacy of the bactericidal composition of the present invention on Alternaria is higher than that calculated by the Colby formula, that is, there is a synergistic effect.

Experiment 2: Scab Test (Apple) / Efficacy Test

Solvent: 24.5 parts by weight of acetone

24.5 parts by weight of dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

In order to obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a certain amount of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test for protective activity, young apple tree plants are sprayed with the active compound preparation at certain application rates. After the sprayed coating dries, young leaves are inoculated with an aqueous spore suspension of A. apple spores. The plants were then placed in an incubator at about 20°C and 100% relative atmospheric humidity for one day; the plants were then placed in a greenhouse at about 20°C and about 90% relative atmospheric humidity. Test results were evaluated 10 days after inoculation. 0% means efficacy equivalent to the control, and 100% efficacy means no symptoms.

Table 2

Table 2 clearly shows that the actual efficacy of the bactericidal composition of the present invention on scab is higher than the efficacy calculated by the Colby formula, that is, there is a synergistic effect.

Experiment 3: Corn Stem Foundation Rot Test

Solvent: 24.5 parts by weight of acetone

24.5 parts by weight of dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with certain amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration of the test drug solution.

Contaminated soil was prepared by mixing the cultured cells of Pythium and Fusarium mixed bacteria cultured in soil wheat bran medium into the test soil.

The above-mentioned test chemical solution was poured into the contaminated soil. After infusion, corn seeds are sown into and covered with contaminated soil. One week after corn sowing, the emergence of corn was observed at any time. After 40 days, the occurrence of corn stalk base rot was observed and recorded.

Calculation method of drug efficacy

table 3

Table 3 clearly shows that the actual efficacy of the bactericidal composition of the present invention on Pythium and Fusarium is higher than that calculated by the Colby formula, that is, there is a synergistic effect.

Test 4: Clubroot test (Chinese cabbage)

Solvent: 24.5 parts by weight of acetone

24.5 parts by weight of dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

In order to obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a certain amount of solvent and emulsifier, and the concentrate is diluted with water to a spray solution of 50 ppm.

A spore suspension of P. rhizogenes (fungal density 1×10 ⁵ fungi/g dry soil) was inoculated into the sterilized soil. Transplant healthy Chinese cabbage (2 true-leaf seedlings) into soil contaminated with P. rhizogenes. On the day of transplanting, 250ml/plant of the above-mentioned test liquid was poured into the contaminated soil. Set clear water control. After 50 days of planting, the roots of cabbage were pulled out and washed, and the clubroot disease of the root system was observed, and the disease index and control effect were calculated.

Calculation method of drug efficacy

Chinese cabbage clubroot disease grading standard:

Grade 0 means no root tumor attached;

Grade 1 is the root tumor attached to the lateral root; the number of root tumor accounts for 1%-25%;

Grade 2: The root swelling on the main root is attached, and the number of root swelling on the lateral root accounts for more than 25%;

Grade 3: There is a root tumor attached to the main root, and the number of root tumor accounts for 50%-75%;

Grade 4 showed that there was swollen root attached to the main root, and the number of swollen roots accounted for more than 75%.

Table 4

Table 4 clearly shows that the actual efficacy of the bactericidal composition of the present invention on P. rhizogenes is higher than that calculated by the Colby formula, that is, there is a synergistic effect.

Claims (6)

1. A germicidal composition, characterized by: the bactericidal composition comprises active compounds of fenpyrazamine and cyazofamid in a weight ratio of 10:1-1:10, preferably 5:1-1:5, and more preferably 1: 1.

2. The germicidal composition of claim 1, wherein: also comprises a filler and/or a surfactant.

3. Use of the fungicidal composition according to claim 1 for controlling pythium species, fusarium species, alternaria species, plasmodiophora species, leptospora species, phytopathogenic fungi and bacteria.

4. A method of controlling plants, plant parts, plant propagation material and subsequently emerging plant organs against the attack by pythium, fusarium, alternaria, plasmodiophora, soft rot fungi and bacteria, characterized in that a fungicidal composition according to claim 1 is applied to the plants, plant parts, plant propagation material or the soil or cultivation medium in which the plants are growing or in need of growth.

5. The method of claim 4, comprising applying the fungicidal composition of claim 1 in an agronomically effective and substantially non-phytotoxic application rate to a plant, plant part, plant propagation material, or soil or cultivation medium in which the plant is growing or in which it is desired to grow, in the form of a seed treatment, foliar application, stem application, drench, pour-on, spray, mist, dusting, scattering, or fuming.

6. The method according to claim 4, characterized in that fenpyrazamine and cyazofamid according to claim 1 are applied simultaneously, or separately, or sequentially.