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CN111803138A - Mask type respiratory tract pathogen sampling device - Google Patents

Mask type respiratory tract pathogen sampling device Download PDF

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Publication number
CN111803138A
CN111803138A CN202010794594.3A CN202010794594A CN111803138A CN 111803138 A CN111803138 A CN 111803138A CN 202010794594 A CN202010794594 A CN 202010794594A CN 111803138 A CN111803138 A CN 111803138A
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pipe
gas
mask
air
collecting
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CN111803138B (en
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刘思聪
张增峰
朱华晨
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4055Concentrating samples by solubility techniques
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B2010/0083Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements for taking gas samples
    • A61B2010/0087Breath samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088Concentrating samples by other techniques involving separation of suspended solids filtration

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
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  • Chemical & Material Sciences (AREA)
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  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
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  • Heart & Thoracic Surgery (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a mask type respiratory tract pathogen sampling device which comprises a mask, a gas delivery pipe, a collecting pipe, a pipe cover, a gas dispersion coil pipe, an exhaust pipe and a refrigeration box, wherein a one-way valve air suction port and an air outlet are arranged on the mask; the collecting pipe is detachably provided with a pipe cover, the pipe cover is provided with an air diffusion coil pipe and an exhaust pipe in an inserted mode, and the air diffusion coil pipe penetrates through the bottom of the collecting pipe; one end of the gas pipe is connected to the gas outlet of the mask, and the other end of the gas pipe is connected to the pipe orifice of the gas diffusion coil pipe; pathogen collecting and preserving fluid is contained in the collecting tube; the collection tube may be fittingly placed within the refrigeration cassette. The invention has the innovation points that the mask intercepts the gas exhaled by a person to be sampled, the gas is led into the air diffusion coil pipe immersed with the sampling preservation liquid, and then the gas is exhausted through the micropores on the spiral air diffusion coil pipe, so that the pathogens carried in the bubbles are dissolved in the pathogen collecting preservation liquid, and the purpose of efficiently collecting the respiratory pathogens is achieved.

Description

一种口罩式呼吸道病原体采样装置A mask-type respiratory pathogen sampling device

技术领域technical field

本发明属于病原体采样用具技术领域,尤其涉及一种口罩式呼吸道病原体采样装置。The invention belongs to the technical field of pathogen sampling appliances, and in particular relates to a mask-type respiratory pathogen sampling device.

背景技术Background technique

目前,呼吸道传染病病原体(如新型冠状病毒、流感病毒、麻疹病毒、结核杆菌等)的采样大多使用拭子刮檫口咽部或鼻咽部的黏膜,然后再将拭子上的病毒洗脱下来用于检测分析。但这种方法存在三个缺点:第一,由于大多数呼吸道传染病的病原体的病灶繁殖点都在下呼吸道的肺部,处于上呼吸道的口咽部和鼻咽部黏膜壁上存留的病原体量较少,因此能刮檫到的病原体也不多;第二,拭子头的表面积非常有限,它所能粘附的黏膜细胞及其病原体也十分有限;第三,要想尽可能地刮擦到病原体,就须将拭子延伸尽量到上呼吸道的后部采集,但这会让被采样者感到非常的不适,特别是采用鼻咽部采样时。因此,亟需发明一种采样工具,既能大量地采集到下呼吸道病灶繁殖点排出的病原体,而且不会给被采样者带来太多的不适。At present, most of the sampling of respiratory infectious disease pathogens (such as new coronavirus, influenza virus, measles virus, Mycobacterium tuberculosis, etc.) use swabs to scrape the mucous membranes of the oropharynx or nasopharynx, and then wash the viruses on the swabs. down for detection and analysis. However, this method has three disadvantages: First, since the foci of most pathogens of respiratory infectious diseases are in the lungs of the lower respiratory tract, the amount of pathogens remaining on the mucosal walls of the oropharynx and nasopharynx in the upper respiratory tract is higher than that of the upper respiratory tract. Therefore, there are not many pathogens that can be scratched; second, the surface area of the swab head is very limited, and the mucosal cells and pathogens it can adhere to are also very limited; third, if you want to scratch as much as possible Pathogens, it is necessary to extend the swab as far as possible to the back of the upper respiratory tract for collection, but this will make the sampled person feel very uncomfortable, especially when the nasopharynx is used for sampling. Therefore, there is an urgent need to invent a sampling tool, which can collect a large number of pathogens discharged from the breeding points of lower respiratory tract lesions without causing too much discomfort to the sampled person.

发明内容SUMMARY OF THE INVENTION

本发明针对上述现有技术的不足,发明了一种口罩式呼吸道病原体采样装置。Aiming at the deficiencies of the above-mentioned prior art, the present invention invents a mask-type respiratory pathogen sampling device.

为了实现上述目的,本发明采用如下的技术方案:In order to achieve the above object, the present invention adopts the following technical scheme:

一种口罩式呼吸道病原体采样装置,包括口罩、输气管、收集管、管盖、散气盘管和排气管,所述的口罩上设置了有单向阀吸气口和出气口;所述的收集管上可拆卸地设置有管盖,所述的管盖上安插有散气盘管和排气管,所述的散气盘管延伸到收集管底部;所述的输气管的一端连接在口罩上的出气口上,输气管另一端连接在管盖上的散气盘管的管口上;所述的收集管内盛装有病原体收集保存液。A mask-type respiratory pathogen sampling device, comprising a mask, an air delivery pipe, a collection pipe, a pipe cover, a diffuser coil and an exhaust pipe, and the mask is provided with a one-way valve suction port and an air outlet; the The collecting pipe is detachably provided with a pipe cover, the pipe cover is provided with a gas diffuser coil and an exhaust pipe, and the gas diffuser coil extends to the bottom of the collecting pipe; one end of the gas transmission pipe is connected to On the air outlet on the mask, the other end of the air delivery pipe is connected to the pipe opening of the air diffuser coil on the pipe cover; the collection pipe is filled with pathogen collection and preservation solution.

本发明的创新点在于:第一,采用设计有微孔的螺旋盘管在溶液中大量地和分散地释放被采样者呼出的气体,从而将气体中携带的病原体富集式地洗脱在病原体收集保存液中;第二,通过佩戴专用口罩将被采样者呼出的气体导入病原体收集管,口罩导出的气体都是来自被采样者的肺部呼气,其中含有大量的病原体;第三,被采样者对佩戴口罩有很好的医从性,可让被采样者较长时间佩戴口罩和洗脱病原体,来提高病原体的采集量。The innovations of the present invention are as follows: First, a spiral coil tube designed with micropores is used to release the gas exhaled by the sampler in a large amount and dispersedly in the solution, so as to enrich the pathogens carried in the gas and elute them in the pathogens. Collect the preservation solution; second, the gas exhaled by the sampled person is introduced into the pathogen collection tube by wearing a special mask, and the gas exported by the mask is all from the lung exhalation of the sampled person, which contains a large amount of pathogens; The sampler has a good medical compliance with wearing a mask, which can allow the sampled person to wear a mask for a long time and wash out pathogens to increase the amount of pathogens collected.

进一步,所述的收集管的管体为实验室常用50ml尖底或圆底离心管,其管盖进行改装,使用常规的离心管,可便利地在后续实验中与其它相关仪器设备相匹配。Further, the tube body of the collection tube is a 50ml pointed-bottom or round-bottom centrifuge tube commonly used in laboratories, and the tube cover is modified to use a conventional centrifuge tube, which can be conveniently matched with other related equipment in subsequent experiments.

进一步,所述的散气盘管的下部呈螺旋状盘绕,散气盘管下部的管壁四周密布有散气孔,受检者呼出的气体在排出散气盘管时,通过众多的、细小的散气孔与病原体收集保藏液充分接触混合,让呼出气体中的病原微生物充分溶解在病原体收集保存液中。Further, the lower part of the air diffusing coil is spirally coiled, and the wall of the lower part of the air diffusing coil is densely covered with air diffusing holes. The diffuser holes are fully contacted and mixed with the pathogen collection and preservation solution, so that the pathogenic microorganisms in the exhaled air are fully dissolved in the pathogen collection and preservation solution.

进一步,所述的散气孔的孔径为0.1~0.3mm,孔径小,呼出气体的气泡就越小,相对来说气泡的表面积也就越大,与病原体收集保存液接触的面积也就越多,溶解在病原体保存液中的机会也就越多。另外,小孔径的设计可让有限的螺旋盘管上螺旋盘管上设置更多的散气孔。Further, the pore size of the air diffuser is 0.1-0.3mm, the smaller the pore size, the smaller the bubbles of exhaled air, the larger the surface area of the bubbles, the more the contact area with the pathogen collection and preservation solution. The greater the chance of dissolution in the pathogen preservation solution. In addition, the design of small aperture allows more air diffusion holes to be set on the limited spiral coil.

进一步,还包括冷藏盒,所述的冷藏盒包括中空的盒体、插槽、腰带和注水口,所述的盒体的顶部设置有用于放置收集管的插槽,可将收集管贴合适配地安放在冷藏盒内;所述的盒体上设置有腰带,方便被采样者舒适地悬挂在身上进行长时间采样;所述的注水口设置在盒体的上表面,通过注水口可往冷藏盒内注入水或其他冷媒,这样在冷藏盒预冷的情况,可让溶解到收集盒内的病原微生物在低温中更好地保存其生物活性。Further, it also includes a refrigerated box, the refrigerated box includes a hollow box body, a slot, a waistband and a water injection port, the top of the box body is provided with a slot for placing the collection tube, and the collection tube can be attached to a suitable It is placed in the refrigerated box; the box body is provided with a belt, which is convenient for the sampled person to hang on his body comfortably for long-term sampling; Water or other refrigerants are injected into the refrigerated box, so that the pathogenic microorganisms dissolved into the collection box can better preserve their biological activity at low temperature when the refrigerated box is pre-cooled.

进一步,还包括止回阀,所述的散气盘管的进气端设置有止回阀,避免收集管内的保藏液回流到输气管内。Further, a check valve is also included, and a check valve is provided at the air inlet end of the air diffusing coil to prevent the preservation liquid in the collection pipe from flowing back into the gas transmission pipe.

进一步,所述的排气管上设置有过滤膜和防水透气膜,在保证收集管内的保藏液不会喷出的情况下,可以对收集管内排出的气体进行过滤,防止病原体逃逸造成环境污染。Further, the exhaust pipe is provided with a filter membrane and a waterproof and breathable membrane. Under the condition that the preservation liquid in the collection pipe is not sprayed out, the gas discharged from the collection pipe can be filtered to prevent the escape of pathogens and cause environmental pollution.

本发明的优点在于:本发明采用口罩收集被采样人从肺部呼出的病原体,再使用病原体收集保存液对呼出的气体进行洗脱处理,使呼出气体中夹带的病原体被洗脱到病原体收集保存液中,达到方便和大量地采集下呼吸道感染病原体的目的。该装置具有结构简单、使用方便、医从性好、可采集下呼吸道感染病原微生物、富集采集量大、可保持病原体生物活性等优点;其结构简单,各个部件能够灵活的拆解,方便运输和使用,制作成本低,实用性强,适合在各种呼吸道传染病的病原体采集。The advantages of the present invention are: the present invention uses a mask to collect the pathogens exhaled from the lungs of the sampled person, and then uses the pathogen collection and preservation solution to elute the exhaled air, so that the pathogens entrained in the exhaled air are eluted to the pathogen collection and preservation. In the liquid, the purpose of collecting the pathogens of lower respiratory tract infection in a convenient and large amount is achieved. The device has the advantages of simple structure, convenient use, good medical compliance, can collect pathogenic microorganisms of lower respiratory tract infection, large amount of enrichment and collection, and can maintain the biological activity of pathogens. And use, low production cost, strong practicability, suitable for pathogen collection in various respiratory infectious diseases.

附图说明Description of drawings

图1是本发明一种口罩式呼吸道病原体采样装置的结构示意图;Fig. 1 is the structural representation of a kind of mask-type respiratory pathogen sampling device of the present invention;

图2是本发明一种口罩式呼吸道病原体采样装置中管盖和散气盘管的结构示意图。FIG. 2 is a schematic structural diagram of a tube cover and a diffuser coil in a mask-type respiratory pathogen sampling device of the present invention.

具体实施方式Detailed ways

以下结合附图对本发明进行详细说明:The present invention is described in detail below in conjunction with the accompanying drawings:

在本发明的描述中,需要说明的是,“左”、“右”、“上”、“下”等指示的方位或位置关系为基于附图所示的方位或位置关系,或者是该发明产品使用时惯常摆放的方位或位置关系,仅是为了便于描述本发明和简化描述,而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作,因此不能理解为对本发明的限制。In the description of the present invention, it should be noted that the orientations or positional relationships indicated by "left", "right", "upper", "lower", etc. are based on the orientations or positional relationships shown in the drawings, or the invention The orientation or positional relationship that is usually placed when the product is used is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be used. It is understood as a limitation of the present invention.

如图1-2所示,一种口罩式呼吸道病原体采样装置,包括口罩1、输气管2、收集管3、管盖4、散气盘管5、排气管6、散气孔7、冷藏盒8和止回阀9,所述的口罩1上设置有单向阀吸气口11和出气口12,所述的收集管3上可拆卸地设置有管盖4,收集管3采用50ml的通用尖底或圆底离心管,在管内设置盛装病原体收集保存液。本实施方式其中一个创新点是采用实验室通用的50ml离心管进行改造,只需要在管盖4上安插设置散气盘管5和排气管6就可。在未使用前,散气盘管5和排气管6的管口都会用橡胶盖盖好,避免其中预装的病原体收集保存液被污染或漏出。收集管的各部件装配完后,所述的散气盘管5延伸至收集管3的下部,在排气管6的管口处设置有过滤膜和防水透气膜,过滤膜位于外侧。所述的输气管2的一端套插在出气口12上,输气管2的另一端与散气盘管5套插连接,散气盘管5在与输气管2连端上设置有止回阀9,可以防止保藏液回流到输气管2内。As shown in Figure 1-2, a mask-type respiratory pathogen sampling device includes a mask 1, an air delivery pipe 2, a collection pipe 3, a pipe cover 4, an air diffuser coil 5, an exhaust pipe 6, an air diffuser hole 7, and a refrigerator box. 8 and a check valve 9, the mask 1 is provided with a one-way valve suction port 11 and an air outlet 12, the collection pipe 3 is detachably provided with a pipe cover 4, and the collection pipe 3 adopts a 50ml universal Conical bottom or round bottom centrifuge tube, set in the tube to hold the collection and preservation solution of pathogens. One of the innovative points of this embodiment is to use a 50ml centrifuge tube commonly used in laboratories for transformation, and it is only necessary to install the air diffuser coil 5 and the exhaust pipe 6 on the tube cover 4 . Before not in use, the nozzles of the diffuser coil 5 and the exhaust pipe 6 will be covered with rubber caps to avoid contamination or leakage of the pre-installed pathogen collection and preservation solution therein. After the components of the collecting pipe are assembled, the air diffuser coil 5 extends to the lower part of the collecting pipe 3, and a filter membrane and a waterproof and breathable membrane are arranged at the nozzle of the exhaust pipe 6, and the filter membrane is located on the outside. One end of the gas transmission pipe 2 is sleeved on the air outlet 12, and the other end of the air transmission pipe 2 is sleeved and connected with the air diffuser coil 5, and the air diffuser coil 5 is provided with a check valve on the connecting end of the air transmission pipe 2. 9. It can prevent the preservation liquid from flowing back into the gas pipe 2.

本实施方式的散气盘管5的末端是封闭的,由直管和螺旋盘管构成,螺旋盘管位于散气盘管5的下部,直管从螺旋盘管中间穿过,直管的上端凸出管盖4,下端连接螺旋盘管的底部。在螺旋盘管与直管的下半部分上设置有散气孔7,散气孔7有序地、密集地遍布其上,散气孔7的孔径为0.2mm。所述的冷藏盒8包括中空的盒体81、插槽82、腰带83和注水口84,所述的盒体81的上部设置有用于放置收集管3的插槽82;所述的盒体81上设置有腰带83;所述的注水口84设置在盒体81的上表面。当使用者需要长时间佩戴收集病原体时,可以从冷藏柜中把预冷的冷藏盒8取出,挂在身上然后把收集管3插上,并连接好输气管2即可。The end of the air diffusing coil 5 in this embodiment is closed, and is composed of a straight pipe and a spiral coil. The spiral coil is located at the lower part of the air diffusing coil 5, and the straight pipe passes through the middle of the spiral coil. The tube cover 4 is protruded, and the lower end is connected to the bottom of the spiral coil tube. Air diffuser holes 7 are arranged on the lower half of the spiral coil tube and the straight tube, and the air diffuser holes 7 are distributed in an orderly and densely manner, and the diameter of the air diffuser holes 7 is 0.2 mm. The refrigerated box 8 includes a hollow box body 81, a slot 82, a waistband 83 and a water injection port 84. The upper part of the box body 81 is provided with a slot 82 for placing the collecting pipe 3; the box body 81 A waist belt 83 is arranged on the upper surface; the water filling port 84 is arranged on the upper surface of the box body 81 . When the user needs to wear it for a long time to collect pathogens, he can take out the pre-cooled refrigerated box 8 from the refrigerator, hang it on his body, plug in the collection tube 3, and connect the air delivery tube 2.

尽管上文对本发明的具体实施方案进行了详细的描述和说明,但应该指明的是,我们可以对上述实施方案进行各种改变和修改,但这些都不脱离本发明的精神和所附的权利要求所记载的范围。Although the specific embodiments of the present invention have been described and illustrated in detail above, it should be noted that various changes and modifications can be made to the above-mentioned embodiments without departing from the spirit of the present invention and the appended rights The scope of the requirement is stated.

Claims (7)

1. A mask type respiratory tract pathogen sampling device is characterized by comprising a mask (1), a gas conveying pipe (2), a collecting pipe (3), a pipe cover (4), a gas diffusion coil pipe (5) and an exhaust pipe (6), wherein a one-way valve gas suction port (11) and a gas outlet (12) are arranged on the mask (1), and the pipe cover (4) is detachably arranged on the collecting pipe (3); the pipe cover (4) is inserted with an air-dispersing coil pipe (5) and an exhaust pipe (6), and the air-dispersing coil pipe (5) extends to the bottom of the collecting pipe (3); one end of the gas pipe (2) is connected to the gas outlet (12) of the mask, and the other end of the gas pipe (2) is connected to the pipe orifice of the gas diffusion coil pipe (5); pathogen collection and preservation solution is contained in the collection pipe (3).
2. The mask type respiratory pathogen sampling device according to claim 1, wherein the collection tube (3) is a conventional pointed-bottom or round-bottom centrifuge tube.
3. The mask type respiratory tract pathogen sampling device according to claim 2, wherein the lower part of the air diffusion coil (5) is spirally coiled, and air diffusion holes (7) are distributed on the periphery of the tube wall.
4. The mask type respiratory tract pathogen sampling device according to claim 3, wherein the aperture of the air diffusion hole (7) is 0.1-0.3 mm
5. The mask type respiratory tract pathogen sampling device according to claim 4, further comprising a refrigeration box (8), wherein the refrigeration box (8) comprises a hollow box body (81), a slot (82), a waistband (83) and a water injection port (84), and the slot (82) for placing the collection tube (3) is arranged on the upper surface of the box body (81); the slot (82) is matched and matched with the collecting pipe (3); a waistband (83) is arranged on the box body (81); the water injection port (84) is arranged on the upper surface of the box body (81).
6. The mask respiratory pathogen sampling device according to claim 5, further comprising a check valve (9), wherein the air inlet end of the air dispersion coil (5) is provided with the check valve (9).
7. The mask type respiratory pathogen sampling device according to claim 6, wherein the exhaust pipe (6) is provided with a filtering membrane and a waterproof breathable membrane.
CN202010794594.3A 2020-08-10 2020-08-10 A mask-type respiratory pathogen sampling device Active CN111803138B (en)

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