CN111450166A - New use of Chaihingzhi in resisting enterovirus - Google Patents

Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a new application of a Chailian preparation in resisting enterovirus. The CHAIHUANG preparation comprises bupleuri radix, flos Lonicerae, Scutellariae radix, radix Puerariae, herba Schizonepetae, herba Artemisiae Annuae, and fructus forsythiae. Clinical research feedback shows that the Bupleurum-silver preparation is simple in preparation process and has already been subjected to large-scale production of an amplification process in reducing the lung index of the lung attacked by enterovirus.

Description

New use of Chaihingzhi in resisting enterovirus

Technical Field

The invention relates to a new application of a traditional Chinese medicine composition, in particular to a new application of a Chaihin preparation in resisting enterovirus.

Background

The Chaihingzhiji oral liquid, Chaihingling granule and other oral medicinal preparation are prepared with ten kinds of Chinese medicinal materials including bupleurum root, honeysuckle, skullcap root, kudzu vine root, schizonepeta, sweet wormwood, forsythia, balloonflower root, bitter apricot seed, mint and cordate houttuynia, and have the functions of clearing away heat, relieving exterior syndrome, dispersing lung qi, clearing away heat, eliminating phlegm, relieving sore throat and stopping cough, and may be used in treating wind-heat exterior syndrome of pathogenic wind attacking lung and defending lung, such as fever, aversion to cold, headache, sore throat, red tongue edge and floating pulse. In the prescription, the bupleurum root is used as a monarch drug for relieving the heat retention and dispersing the dispersing function of the lung and dispersing the exterior heat; honeysuckle, scutellaria, forsythia, kudzu root, sweet wormwood and schizonepeta, and the six medicines have the effects of clearing heat, relieving exterior syndrome, ventilating lung, discharging heat, ventilating muscle and relieving fever and are used as ministerial medicines; mint, houttuynia cordata and bitter apricot kernel, which are used as adjuvant drugs for relieving sore throat, quenching thirst, clearing lung-heat, eliminating heat, reducing phlegm and relieving cough; the root of balloonflower is specialized in entering lung meridian, dispersing lung qi and resolving phlegm, relieving sore throat and stopping cough, and all the herbs in the carrier formula directly enter lung system to reach the effect of dispersing pathogenic factors and act as guiding drugs. The whole formula takes the pungent and cool dispersing powder as the main component and is matched with the medicinal flavors of sweet cold, bitter cold and pungent warm, thereby not only enhancing the effect of dispersing and clearing, but also having no disadvantage of bitter cold over-lingering the evil into the interior; the combination of pungent-cool, bitter-cold and pungent-warm herbs in the recipe strengthens the actions of ventilating the lung, dispersing pathogenic factors and relieving exterior syndrome, and can correct the bias of bitter-cold, sweet-cold and over-cold without the disadvantage of warm-dryness.

Disclosure of Invention

In the clinical medication process of years, the bupleurum-silver preparation, especially the bupleurum-silver particle/oral liquid has obvious treatment effect on enterovirus, especially viruses causing hand-foot-and-mouth disease and obvious curative effect on the hand-foot-and-mouth disease, wherein the application of the externally applied medicine for treating the hand-foot-and-mouth disease is disclosed.

The traditional Chinese medicine composition related to the patent is patented at 22.06.2005 (No. CN 1207033C), and a series of Chaiyin preparations including Chaiyin granules, Chaiyin oral liquid and the like which are on the market are prepared by relying on the patent technology, and the preparation products are prepared from radix bupleuri, honeysuckle, scutellaria baicalensis, radix puerariae, herba schizonepetae, sweet wormwood herb, fructus forsythiae, platycodon grandiflorum, bitter almond, mint and herba houttuyniae.

Specifically, the present invention is such that:

the first purpose of the invention is to provide the application of a traditional Chinese medicine composition mainly prepared from radix bupleuri, honeysuckle, scutellaria baicalensis, radix puerariae, schizonepeta, sweet wormwood, fructus forsythiae, bitter apricot seed, mint, houttuynia cordata and platycodon grandiflorum in preparing a medicine for treating enterovirus.

The traditional Chinese medicine composition comprises the following components in parts by weight:

25-75 parts of bitter almond, 40-110 parts of mint, 40-110 parts of houttuynia cordata

25-75 parts of platycodon grandiflorum.

The traditional Chinese medicine composition of the invention can also comprise the following raw material medicines in parts by weight:

80-120 parts of bupleurum root, 60-90 parts of honeysuckle flower, 50-70 parts of scutellaria root

40-60 parts of kudzu root, 40-60 parts of schizonepeta, 60-90 parts of sweet wormwood herb

60-90 parts of fructus forsythiae, 40-60 parts of bitter almond, 60-90 parts of mint

60-90 parts of houttuynia cordata and 40-60 parts of orange stem.

The traditional Chinese medicine composition is prepared from the following raw material medicines in percentage by weight:

bupleurum root 100 weight portions, honeysuckle 75 weight portions, scutellaria 60 weight portions

50 parts of kudzuvine root, 50 parts of schizonepeta, 75 parts of artemisia apiacea

75 parts of fructus forsythiae, 50 parts of platycodon grandiflorum and 50 parts of bitter apricot kernel

75 parts of mint and 75 parts of houttuynia cordata.

The enterovirus may be one of Cox a16 and EV 71.

The enterovirus is any one of herpes myocarditis, pulmonary edema, aseptic meningoencephalitis, herpangina, recurrent oral ulcer and herpes systemically.

The Chinese medicinal composition comprises the following medicinal ingredients:

bupleurum root: bitter in property and slightly cold in nature.

Meridian tropism: it enters liver and gallbladder meridians.

The functional indications are as follows: harmonize exterior and interior, soothe liver and raise yang. Can be used for treating common cold, fever, alternating chills and fever, distending pain in chest and hypochondrium, menoxenia, prolapse of son and sense organs, and proctoptosis.

Fructus forsythiae: bitter in property and slightly cold in nature.

Meridian tropism: it enters lung, heart and small intestine meridians.

The functional indications are as follows: clear heat and remove toxicity, resolve swelling and dissipate nodulation. Can be used for treating carbuncle, cellulitis, scrofula, acute mastitis, erysipelas, wind-heat type common cold, early epidemic febrile disease, warm heat entering nutrient system, hyperpyrexia, polydipsia, coma, macula, and stranguria with heat.

Bitter apricot seeds: bitter in nature and slightly warm in nature; has little toxicity.

Meridian tropism: it enters lung and large intestine meridians.

The functional indications are as follows: direct qi downward to relieve cough and dyspnea, moisten intestines to relieve constipation. Can be used for treating cough, asthma, chest fullness, excessive phlegm, blood deficiency, dry body fluid, intestinal dryness, and constipation.

Mint: pungent and cool in nature and taste.

Meridian tropism: it enters lung and liver meridians.

The functional indications are as follows: disperse wind-heat. Clear head and eyes, promote eruption. Can be used for treating wind-heat type common cold, headache, conjunctival congestion, pharyngitis, aphtha, rubella, measles, and chest and hypochondrium swelling.

Houttuynia cordata: pungent in nature and slightly cold in flavor.

Meridian tropism: it enters lung meridian.

The functional indications are as follows: clearing away heat and toxic material, curing abscess and discharging pus, inducing diuresis and treating stranguria. Can be used for treating lung abscess with purulent vomiting, phlegm heat, cough and asthma, dysentery, pyretic stranguria, carbuncle, swelling, and sore.

Balloon flower: bitter, pungent and mild in nature.

Meridian tropism: it enters lung meridian.

The functional indications are as follows: disperse lung qi, relieve sore throat, dispel phlegm, and expel pus. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, suppuration, and pyocutaneous disease with pus formation.

Honeysuckle flower: sweet in nature and taste and cold in nature.

Meridian tropism: it enters lung, heart and stomach meridians.

The functional indications are as follows: clear heat and remove toxicity, cool and disperse wind-heat. Can be used for treating carbuncle, furuncle, sore throat, erysipelas, toxic heat, dysentery, wind-heat type common cold, epidemic febrile disease, and fever.

Scutellaria baicalensis: bitter in property and cold in nature.

Meridian tropism: it enters lung, gallbladder, spleen, large intestine and small intestine meridians.

The functional indications are as follows: clear heat and dry dampness, purge fire and remove toxicity, stop bleeding, prevent abortion. Can be used for treating chest distress, nausea, damp-heat, dysentery, jaundice, cough due to lung heat, hyperpyrexia, polydipsia, hematemesis, carbuncle, swelling, sore, and threatened abortion.

Kudzu root: sweet, pungent and cool in nature and taste.

Meridian tropism: it enters spleen and stomach meridians.

The functional indications are as follows: expelling pathogenic factors from muscles and skin, allaying fever, promoting salivation, promoting eruption, invigorating yang, and relieving diarrhea. Can be used for treating fever, headache, stiffness and pain of neck and back, thirst, diabetes, measles without adequate eruption, dysentery, and diarrhea; hypertension neck and neck pain.

Herba schizonepetae: pungent in nature and slightly warm in flavor.

Meridian tropism: it enters lung and liver meridians.

The functional indications are as follows: relieve exterior syndrome, dispel wind and promote eruption. Can be used for treating common cold, headache, measles, rubella, and initial stage of pyocutaneous disease. Stir-baked into charcoal is indicated for hematochezia, metrorrhagia and metrostaxis and postpartum anemic fainting.

Sweet wormwood herb: bitter, pungent and cold in nature.

Meridian tropism: it enters liver and gallbladder meridians.

The functional indications are as follows: clear away summer-heat, remove heat and stop malaria. Can be used for treating fever due to summer-heat, fever due to yin deficiency, night fever with early coolness, hectic fever due to yin deficiency, malaria with cold-heat, and jaundice due to damp-heat.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The invention is further illustrated by the following examples, which should be properly understood: the examples of the present invention are merely illustrative and not restrictive, and therefore, the present invention may be modified in a simple manner without departing from the scope of the invention as claimed.

The following examples can achieve the effects of the above experimental examples:

test example 1 therapeutic Effect of drugs on Enterovirus

In order to research the therapeutic effect of the silver particles on enteroviruses, two different viruses Cox A16 and EV 71 are respectively selected to carry out related experiments, the inventor carries out animal and in vitro experimental research, experiments BA L B/c mice and male and female halves respectively, and animal experimental research methods and results are as follows.

1 test Material

1.1 test drugs

Name (R)	Providing a unit
		Chaiyin oral liquid	LUNAN HOPE PHARMACEUTICAL Co.,Ltd.

1.2 Experimental animals

BA L B/c mice

1.3 strains and cells

1.3.1 viral strains: cox A16 and EV 71 viruses, provided by institute of medical and biotechnology, of Chinese academy of medical sciences, were passaged in the laboratory and stored in a refrigerator at-80 ℃ for later use.

1.3.2 cell lines: human lung cancer cell A549, purchased from Beijing Beinan Chuanglian union of Industrial science, laboratory passage, and stored in liquid nitrogen for later use.

2 items of examination

In vitro virus inhibition rate, mouse peripheral blood T lymphocyte and B lymphocyte ratio (flow cytometry detection), lung tissue pathological examination and other items

2.1 statistical analysis

Statistical analysis is carried out by using SPSS17.0 software, differences among groups are subjected to ANOVA, L SD test is adopted when the variances are uniform, Dunnett T3 test is adopted when the variances are irregular, and P <0.05 has statistical significance.

3 results of the experiment

General sign observation, lung index and inhibition rate, the influence of mouse peripheral blood lymphocyte percentage and the influence of mouse lung tissue pathology prove that the Chaihingyin oral liquid has the obvious effect of treating fever and cough caused by two viruses of Cox A16 and EV 71, and in vitro experiments show that the Chaihingyin oral liquid has the effect of obviously inhibiting two viruses of Cox A16 and EV 71.

Experimental example 2 clinical cases demonstrated therapeutic effects of Chailian oral liquid on patients with enterovirus

1 test Material

1.1 test drugs

Name (R)	Providing a unit
		Chaiyin oral liquid	LUNAN HOPE PHARMACEUTICAL Co.,Ltd.

The health recovery condition of the patient is observed, and the externally-washing or internally-taking of the Chaihingyin oral liquid is proved to have obvious treatment effect.

Example 1:

bupleurum root 250g honeysuckle flower 187.5g baikal skullcap root 150g

Kudzu root 125g Schizonepeta mustard 125g artemisia green 187.5g

187.5g of fructus forsythiae, 125g of platycodon grandiflorum, 125g of bitter apricot kernel

187.5g mint, 187.5g houttuynia cordata

Crushing bitter apricot kernel into coarse particles, soaking seven materials such as radix bupleuri, honeysuckle, sweet wormwood, fructus forsythiae, herba schizonepetae, mint, houttuynia cordata and the like in water for 1 hour, heating and extracting volatile oil, storing the extracted volatile oil in another container, filtering out liquid medicine, and storing in another container; mixing the residue with Scutellariae radix, radix Puerariae, and radix Platycodi, decocting in water for three times, adding semen Armeniacae amarum decoction 3 times after boiling, each for 2 hr, mixing the decoction with the above medicinal liquid, concentrating to relative density of 1.11-1.14 (50 deg.C), filtering, concentrating the filtrate, adding ethanol, stirring, refrigerating for 24 hr, collecting supernatant, recovering ethanol under reduced pressure, concentrating to relative density of 1.35-1.40 (50 deg.C), oven drying (70 deg.C), adding pharmaceutically acceptable adjuvants, and granulating by conventional method.

Claims (9)

1. A Chinese medicinal composition for treating diseases caused by enterovirus is prepared from bupleuri radix, flos Lonicerae, Scutellariae radix, radix Puerariae, herba Schizonepetae, herba Artemisiae Annuae, fructus forsythiae, semen Armeniacae amarum, herba Menthae, herba Houttuyniae, and radix Platycodi.

2. The use of claim 1, wherein the Chinese medicinal composition is prepared from the following raw material medicines in parts by weight:

80-120 parts of bupleurum root, 60-90 parts of honeysuckle flower, 50-70 parts of scutellaria root

40-60 parts of kudzuvine root, 40-60 parts of schizonepeta, 60-90 parts of sweet wormwood herb

60-90 parts of fructus forsythiae, 40-60 parts of bitter almond, 60-90 parts of mint

60-90 parts of houttuynia cordata, 40-60 parts of platycodon grandiflorum.

3. The use of claim 1, wherein the Chinese medicinal composition is prepared from the following raw material medicines in parts by weight:

100 parts by weight of radix bupleuri, 75 parts by weight of honeysuckle flower, 60 parts by weight of radix scutellariae

50 parts of kudzuvine root, 50 parts of fineleaf schizonepeta herb, 50 parts of sweet wormwood herb and 75 parts of sweet wormwood herb

75 parts of fructus forsythiae, 50 parts of bitter almond and 75 parts of mint

75 parts of houttuynia cordata and 50 parts of platycodon grandiflorum.

4. The use of claim 1, wherein the enterovirus is one of Cox a16 and EV 71.

5. The use according to claim 1, wherein the disease caused by enterovirus is any one of herpes myocarditis, pulmonary edema, aseptic meningoencephalitis, herpangina, recurrent oral ulcer, and systemic herpes.

6. A pharmaceutical preparation for treating enterovirus, which is prepared from the traditional Chinese medicine composition of any one of claims 1 to 3, or is prepared from the traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.

7. The pharmaceutical preparation according to claim 6, wherein the dosage form of the pharmaceutical preparation is any one selected from oral liquid, granules, tablets, capsules, lotion, mixture, pills, dripping pills, solid dispersion, ointment, emulsion, chewable agent, injection, traditional Chinese medicine film or microcapsules.

8. The pharmaceutical preparation according to claim 6, wherein the dosage form of the pharmaceutical preparation is one of oral liquid and granules.

9. The pharmaceutical preparation of claim 7 or 8, wherein the oral liquid is Chaihsilver oral liquid, and the granules are Chaihsilver granules.