CN1207033C - Traditional Chinese medicine composition for curing wind-heat type cold and its preparing method - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for treating anemopyretic cold and a preparation method thereof. The composition is composed of bupleurum, honeysuckle, scutellaria baicalensis, kudzu root, nepeta, artemisia annua, forsythia, bellflower, bitter almond, mint and houttuynia cordata. When the traditional Chinese medicine composition is prepared, the methods of distillation, decoction and ethanol extraction are adopted for different components, so as to fully exert the effective medicine. The composition has good effect on treating wind-heat and cold.

Description

A kind of traditional Chinese medicine composition for treating anemopyretic cold and preparation method thereof

field of invention

The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating anemopyretic cold.

Background technique

Anemopyretic cold is one of the most common diseases of human beings, with a high incidence rate, which can easily cause other diseases such as myocarditis, which brings a lot of inconvenience to people's life and work.

Flu is a common exogenous disease caused by pathogenic wind attacking the human body. The records about colds were first seen in the "Nei Jing", "Su Wen Gu Kong Lun" "The wind enters from the outside, making people feel cold, sweating, headache, and body weight with aversion to cold." It shows that a cold is mainly caused by exogenous pathogenic wind, which is consistent with the view in "Su Wen·Tai Yin Yang Ming Lun": "Those who are injured by the wind should be affected first". In the later Han Dynasty, Zhang Zhongjing divided the external syndromes into external syndromes and external syndromes in "Treatise on Febrile Diseases: Distinguishing Taiyang Disease Pulse Syndrome and Treating", which laid a theoretical foundation for the dialectical treatment of colds. The etiology and characteristics of colds were discussed in depth in Sui Dynasty's "On the Origin and Symptoms of Various Diseases · Seasonal Diseases and Disorders". "The Origin and Symptoms of Various Sores on Wind-Heat Diseases" has also described the causes and clinical characteristics of wind-heat due to external pathogenesis. The Yuan Dynasty's "Danxi Xinfa · Moderate Cold Appendices" put forward the two principles of not mistaking colds for typhoid fever, and treating colds with pungent warmth and pungent coolness. "Rejuvenation of all diseases · typhoid with wind" pointed out: "Those who have colds and colds at four seasons should relieve the exterior." "Zheng Zhi Hui Bu · Cold" pointed out that colds are not only attacked by wind and evil, but also related to the strength and nature of physical fitness. Ye Tianshi pointed out that "to suffer from warm evils, the first choice is to attack the lungs". To sum up, it can be seen that the etiology of a cold is mainly due to the feeling of wind evil, but it is often closely related to the strength of the human body's righteousness. The location of the disease is mainly on the surface of the lung and guard muscles, and the treatment should be mainly to relieve the surface and diverge. In dialectics, the wind-cold and wind-heat syndromes should be differentiated first, and then according to the physical quality and symptoms, it should be classified as deficiency or reality. He Xinliang explained the appearance as a rule.

Contents of the invention

One object of the present invention is to disclose a new Chinese medicine composition for treating Fengreganmao; another object of the present invention is to disclose a method for preparing a new Chinese medicine composition for treating Fengreganmao.

The crude drug composition and proportioning of pharmaceutical composition of the present invention are as follows (by weight):

Bupleurum 50-150 parts by weight, Honeysuckle 40-110 parts by weight, Scutellaria baicalensis 30-90 parts by weight

Pueraria 25-75 parts by weight Nepeta 25-75 parts by weight Artemisia annua 40-110 parts by weight

Forsythia 40-110 parts by weight

The bulk drug of the following components can also be added in the bulk drug of the pharmaceutical composition of the present invention:

25-75 parts by weight of bitter almonds, 40-110 parts by weight of peppermint, and 40-110 parts by weight of Houttuynia cordata.

25-75 parts by weight of Platycodon grandiflorum can also be added to the crude drug of the pharmaceutical composition of the present invention.

The raw material composition and the proportioning ratio of the pharmaceutical composition of the present invention can also be (by weight):

Bupleurum 80-120 parts by weight, Honeysuckle 60-90 parts by weight, Scutellaria baicalensis 50-70 parts by weight

Pueraria 40-60 weight parts Nepeta 40-60 weight parts Artemisia annua 60-90 weight parts

Forsythia 60-90 parts by weight Bitter almond 40-60 parts by weight Mint 60-90 parts by weight

Houttuynia cordata 60-90 weight parts Bellflower 40-60 weight parts

The optimum weight proportion of above-mentioned raw material is:

100 parts by weight of bupleurum 75 parts by weight of honeysuckle 60 parts by weight of baicalin

50 parts by weight of kudzu root 50 parts by weight of Nepeta 75 parts by weight of Artemisia annua

75 parts by weight of forsythia 50 parts by weight of bitter almonds 75 parts by weight of peppermint

Houttuynia cordata 75 parts by weight Bellflower 50 parts by weight

According to the conventional preparation process, the above raw materials can be prepared into common clinical pharmaceutical dosage forms, such as: pills, tablets, granules, oral liquids, capsules, ointments, emulsions, chewing agents, injections and traditional Chinese medicine films, etc.

The medicine of the present invention can also be added with conventional pharmaceutical excipients, such as solvents, disintegrants, flavoring agents, preservatives, colorants, dispersants, binders, thickeners, lubricants, diluents, etc., as required.

The preparation method of this pharmaceutical composition:

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Artemisia annua, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1-2 hours, heat and distill, collect the distillate for Re-distill, collect the heavy distillate and store it in another container, combine the medicinal residue with the three flavors of baicalin, pueraria radix, and platycodon, add water and decoct 3-4 times, each time for 1-3 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter After that, the filtrate is concentrated; add ethanol, stir well, refrigerate for 22-26 hours, take the supernatant to recover ethanol under reduced pressure, add the above red distillate, and finally go through conventional procedures directly or add pharmaceutically acceptable excipients to make clinical Acceptable dosage forms, such as tablets, oral liquid preparations, capsules, granules, etc.

The composition of the present invention has very good antibacterial and antiviral effects, and has good curative effect for treating diseases such as fever and cough caused by exogenous wind-heat, influenza, viral cold, etc. The composition has very little toxicity and is suitable for clinical application. Safe and reliable.

The composition of the invention has obvious antibacterial effect on common respiratory bacteria in vitro, and has obvious antiviral effect on both types A and B of influenza virus. It has obvious antipyretic effect on rats and rabbits, and has obvious antitussive effect on mice and guinea pigs. It can obviously promote the non-specific immunity of mice. The following experimental examples are used to further illustrate the present invention. The following experimental materials and methods are applicable to each experimental example.

One. Experimental material: 1. Tested medicine: composition preparation of the present invention (chaiyin oral liquid, is a compound preparation). Shandong Institute of Traditional Chinese Medicine manufacture room provides, every contains crude drug 7.35g/10ml, according to institute's during the experiment Concentration dilution is required. Batch number: 960216. Cold and cough syrup, product of Guangxi Yulin Pharmaceutical Factory, batch number: 951021; 2 animals: mouse, Kunming species, provided by Shandong Provincial Medical Experimental Animal Center, certificate number, Lushi dynamic quality character: 950101 .Wistar species of rat, Experimental Animal Center of Shandong Medical University, certificate number: 950102.

2. Experimental methods and results: According to the requirements of the "New Drug Approval Measures" and referring to the "Guiding Principles for Preclinical Research of New Chinese Medicines (Discussion Draft)", the main pharmacodynamic experimental observations were conducted, and the results were statistically compared between groups by T or X ² test.

Experimental Example 1 The effect of eliminating pathogenic factors

1.1 Antibacterial test: 1.1.1 Experimental therapeutic effect in animals: 64 mice weighing 20-22g, half ♂♀ in half, were randomly divided into four groups, each group had 8 ♂♀ each, and after 24 hours of grouping, they were given Chaiyin The oral liquid is 3.68g/kg, 7.36g/kg (dosage is calculated according to the amount of the original drug), and the 3.68g/kg group is administered after doubling the Chaiyin oral liquid. The cold and cough syrup is 7.36g/kg, the control group is water with the same volume, and the administration volume is 0.2ml/kg. After 24 hours of administration, according to the pre-test results, give 2.5×10 ⁸ 5% yeast solution per ml, 0.2ml.ip per 10g. Sun Qihua, the technician in charge, provided and assisted in the experiment. At the same time, the drug was still given ig, once a day, for a total of 4 times. The death of the mice was observed, and the results are shown in Table 1, and the X ² test was used for statistics between groups.

Table 1 Protective effect on mice infected with pneumococcus

Group Dose Rat number Death Total mortality %

                                                                                                                                             

Water control 16 4 13 92.86

Oral solution 3.68 16 1 5 31.25

Oral solution 7.36 16 1 3 18.75

Syrup 7.36 16 2 6 32.5

The experimental results showed that Chaiyin Oral Liquid and Ganmao Zhike Syrup had a significant protective effect on pneumococcus-infected mice, reducing their mortality within 48 hours. After 48h-7 days, the mice in each group did not die again.

1.1.2 Antibacterial test in vitro (determination of the minimum inhibitory concentration MIC): Cui Shuyu and Sun Qihua, the chief technicians of the strain room of the Shandong Provincial Epidemic Prevention Station, used the test tube double dilution method to test the effect of Chaiyin oral liquid and cold cough syrup on common respiratory diseases. The measurement result of the minimum inhibitory concentration MLC of bacteria is shown in Table 2:

Table 2 MLC (Minimum Inhibitory Concentration) to common respiratory pathogens

Strains Chaiyin Oral Liquid Cold Cough Syrup

Beta hemolytic strep 1:4(-)1:8(-)1:16(-)1:32(+) 1:4(-)1:8(-)1:16(-)1:32 (+)

Pneumococcus 1:4(-)1:8(-)1:16(+) 1:4(-)1:8(+)

Staphylococcus aureus 1:4(-)1:8(-)1:16(-)1:32(+) 1:4(-)1:8(+)

Escherichia coli 1:2(-)1:4(-)1:16(+) 1:1(-)1:2(+)

The experimental results showed that Chaiyin Oral Liquid and Syrup had a certain antibacterial effect on common pathogenic bacteria in the respiratory tract.

1.2 Antiviral test

1.2.1 Experimental treatment in animals: 64 mice with a body weight of 20-22g, ♂♀ in half, divided into four groups at random, the group administration is the same as in 1.1, 24 hours after the first administration, and 30 minutes after the second administration according to the pre-test LD90 influenza A virus H3N2 strain, intranasally dripped (10ul per mouse), the experimental virus was provided by the Department of Virology, Department of Health, Shandong Medical University, and lecturers Han Guangbin and Wei Yu assisted in the experiment. After the administration of the virus, the administration was continued, once a day, for 4 consecutive days, and the mortality rate of the infected mice in each group was observed and recorded at different times. The results are shown in Table 3, and the comparison between the groups was carried out by the X ² test.

Table 3. The protective effect of Chaiyin Oral Liquid on mice infected with influenza virus

Group Dose (g/kg) Rat number Death Total mortality rate (%)

Total

Control 16 4 4 7 0 15 93.75

Oral solution 3.68 16 1 3 4 0 8 50.00

Oral solution 7.36 16 0 1 4 0 5 31.25

Syrup 7.36 16 2 2 4 0 8 50

After 72 hours, the infected mice in each group did not die again. The experimental results showed that Chaiyin Oral Liquid had a significant protective effect on influenza virus-infected mice and significantly reduced their mortality.

1.2.2 Antiviral test in vitro: The test was assisted by the Department of Health and Virology Department of Shandong Medical University. The virus is influenza virus type A H3N2 and type B standard strain, and the cells are primary human embryonic kidney cells. For drug cytotoxicity test, select well-differentiated human embryonic kidney cell plates covered with a single layer, add different concentrations of oral liquid and syrup to dilute the drug solution to 7 concentrations, 3 experimental plates for each concentration, and culture them at 37°C for 7 days. Days, observed cell lesions every day, calculated the maximum non-toxic concentration TCO, the half toxic concentration TC50, and set up a cell control without adding drugs. The results are shown in Table 4 below:

        Table 4 Drug Cytotoxicity Determination

TC90 TC50 TCO

Syrup 0.04 0.006 0.0006

Oral solution 0.28 0.06 0.006

Note: TC90 is the 90% toxic concentration, TC50 is the 50% toxic concentration, and TCO is the maximum non-toxic concentration; the concentration is represented by crude drug per milliliter, ie g/ml.

Anti-virus test, cytopathic inhibition method, select vigorously growing human embryonic kidney cells, add 100TCID50 virus solution, absorb 2 hours at 37°C 5% _CO2 , suck the virus, add 7 times the maximum non-toxic concentration of the drug solution 2 times dilution Concentrations (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128) were cultured at 37°C for 96 hours, observed cell lesions every day, and recorded the degree of lesions. Cytopathies were observed in the control group and the experimental group at the same time. Calculate the 50% inhibitory lesion concentration IC50, the minimum effective concentration MIEC,

And the therapeutic index TI is shown in Table 5 below:

                                     

IC50 MIEC TI IC50 MIEC TI

Sugar 0.000038 0.000075 8 0.00038 0.00075 8

Oral solution 0.00014 0.000168 32 0.00019 0.000375 16

The experimental results show that the antiviral therapeutic index of Chaiyin Oral Liquid is higher than that of Cold and Cough Syrup.

Experimental example 2, heat-clearing effect

1.1 The antipyretic effect on rats with fever caused by 2.4 dinitrophenol: 40 rats of 150-170g, half of ♂♀, divided into four groups randomly according to body weight and sex, and given Chaiyin oral liquid 2.5g/kg, 5g/kg cold and cough syrup 5g/kg, the control group was given the same volume of water for gavage, the administration volume was 1m, 1/100kg, and the administration was given once. Measure the normal body temperature 10 minutes after the drug, and after 40 minutes, give 2.5% 2.4 dinitrophenol 25mg/kgSC, measure the body temperature of the rats at 0.5, 1, 2, and 4 hours after injection, and compare the body temperature of the rats at different times in each group and inject 2.4 The difference between rat body temperature and normal body temperature after dinitrophenol, the results are shown in Tables 6 and 7:

Table 6 Effect on body temperature of rats induced by 2.4 dinitrophenol X±SD

Dosage Body temperature (°C)

Group (g/kg) Normal 0.5h 1h 2h 4h

Control 37.86±0.47 38.88±0.60 39.62±0.66 39.81±0.67 38.46±0.56

Oral solution 2.5 37.79±0.43 38.76±0.25 39.34±0.29 39.24±0.33* 38.28±0.42

Oral solution 5 37.87±0.36 38.64±0.75 38.80±0.58 39.20±0.20 38.68±0.29

Syrup 5 37.76±0.56 38.76±0.77 39.33±0.45 39.42±0.59 38.36±0.31

Compared with water control group *P<0.05 Compared with syrup group ΔP<0.05

      Table 7 Effects on difference in body temperature of febrile rats X±SD

Dosage Body temperature (°C)

Group (g/kg) 0.5h 1h 2h 4h

Control 1.02±0.33 1.76±.0.43 1.95±0.53 0.8±0.38

Oral solution 2.5 0.97±0.37 1.55±0.37 1.55±0.43 0.49±0.43

Oral solution 5 0.87±0.39 0.93±0.63 1.33±0.30* 0.31±0.35

Syrup 5 1.00±0.34 1.57±0.41 1.66±0.40 0.60±0.40

Compared with water control group *P<0.01

The experimental results show that Chaiyin Oral Liquid has a significant antipyretic effect on rats with 2.4 dinitrophenol fever, and significantly reduces the peak temperature.

2.2 The antipyretic effect on the fever caused by the triple bacterin vaccine in rabbits: 2-2.5kg rabbits (New Zealand species), provided by Shandong Medical Experimental Animal Center, certificate number Lu Shidong 950101. 40 animals that passed the screening were used for both ♂♀. After fasting for 12 hours, they were given triple vaccines of typhoid fever and typhoid fever A and B (produced by Shanghai Institute of Biology, Ministry of Health, batch number 951012) by ear vein injection of 0.75ml/kg. Pre-injection records Normal body temperature, measure the body temperature of each rabbit 60 minutes after injection, select 32 rabbits with a temperature increase of 0.8-1.2°C, and randomly divide them into 4 groups, give Chaiyin oral liquid 0.9g/kg, 1.8g/kg, syrup 1.8g/kg, and the control group Give the same volume of water 4ml/kg once, record the body temperature of the rabbits 1, 3, and 5 hours after the medicine, and calculate the difference between the body temperature of each rabbit and the normal temperature. The results are as follows:

Table 8-1 Effects on body temperature of feverish rabbits X±SD℃

Dosage Body temperature (°C)

Group (g/kg) Normal Injection vaccine 1h 3h 5h

Control 39.04±0.36 40.02±0.42 40.40±0.56 40.41±0.82 39.46±0.62

Oral solution 0.98 39.11±0.44 40.10±0.37 40.21±0.76 39.93±0.74 39.37±0.67

Oral solution 1.8 39.07±0.41 40.01±0.26 40.12±0.64 39.49±0.78 39.18±0.67

Syrup 1.8 39.12±0.38 40.07±0.44 40.18±0.71 39.96±0.67 39.36±0.76

The data of each group in the table were compared by T test. One hour after the injection of the vaccine, the body temperature of each group was significantly higher than the normal body temperature before injection, P<0.05. The body temperature of the water control group was still significantly higher than that before the pyrogenicity, indicating that the triple vaccine had obvious pyrogenic effect. The body temperature of rabbits in the three groups showed a downward trend 2-4 hours after administration.

     Table 8-2 The antipyretic effect of the triple vaccine "fever" in rabbits X±SD

Group Body temperature rise after dose of medicine ℃

(g/kg) 1 2 3 4h

Water control 1.12±0.21 0.81±0.24 1.06±0.26 0.74±0.28

Oral solution 0.9 0.94±0.36 0.32±0.41* 0.53±0.28* 0.40±0.34*

Oral solution 1.8 0.88±0.26 0.12±0.47* 0.36±0.46* 0.28±0.44*

Syrup 1.8 0.94±0.36 0.40±0.41* 0.54±0.21* 0.43±0.41*

Compared with water control group *P<0.05

The results showed that Chaiyin Oral Liquid had obvious cooling effect.

Experimental Example 3. Antitussive effect

3.1 Ammonia-induced cough in mice: 48 mice with a size of 20-22 g passed the screening, half of them for ♂♀, divided into groups and administered the same as 1.1.1, administered once. After 90 minutes of administration, the mice were put into a 1L beaker, given 60% ammonia water, sprayed with ultrasonic atomization for 15 seconds, and recorded the incubation period of the first cough of the mice and the number of times within 2 minutes. The results are as follows:

Table 9 Antitussive effect on mice induced by ammonia water X±SD

Group Dose Number of Rats Incubation Period S Times

Control 12 47.83±23.46 11.83±8.36

Oral solution 3.68 12 80.16±16.24*** 4.78±2.41**

Oral solution 7.36 12 88.48±28.40*** 2.06±2.35***ΔΔ

Syrup 7.36 12 70.45±26.22* 9.00±7.55

Compared with water control group *P<0.05**P<0.01***P<0.001 Compared with syrup group ΔΔ P<0.01

The experimental results show that Chaiyin oral liquid has obvious antitussive effect, and its effect is significantly better than cold cough syrup at the same dose, reducing the number of coughs.

3.2 Cough induction test of guinea pigs with 17.5% citric acid: 40 guinea pigs with a weight of 150-200g were screened and used for both ♂♀. They were randomly divided into 4 groups. kg gavage, the control group was given the same volume of water, and the administration volume was 1ml/100g. After 90 minutes of administration, the guinea pigs were put into a 4L glass desiccator and given 17.5% citric acid ultrasonic atomization spray for 15S. Record the incubation period of the first cough and the number of coughs within 5 minutes of the genus, and the results are shown in the table below.

       Table 10 Antitussive effect on guinea pigs induced by citric acid

Group Dose g/kg Number of rats Incubation period S Cough times

Water control 10 50.16±16.32 15.88±4.76

Oral solution 1.5 10 76.48±29.02* 7.47±3.64**Δ

Oral solution 3 10 70.00±20.31* 7.84±2.36**Δ

Syrup 3 10 63.89±14.4 10.94±2.47*

Compared with water control group *P<0.05**P<0.01, compared with syrup group ΔP<0.05

The experimental results prove that Chaiyin oral liquid has obvious antitussive effect on irritating cough, and its effect is obviously better than cold cough syrup at the same dose.

Experimental example 4. Anti-inflammatory effect

4.1 The effect on ear swelling of mice: 48 mice with a weight of 20-22g, both for ♂♀, were randomly divided into 4 groups. Give 2% compound croton oil 20ul to apply to both sides of the ears (front and back), respectively. After 4 hours, use a hole punch with a diameter of 9mm to punch the left and right ear pieces of the mouse, and weigh them with an electronic balance of 1/10,000. The degree of swelling is obtained by subtracting the weight of the left ear piece. The results are shown in the table below.

      Table 11 Effects on mouse ear swelling X±SD

Group Dose g/kg Number of mice Swelling

Control 12 27.07±4.16

Oral solution 3.68 12 17.08±3.43*

Oral solution 7.36 12 17.42±4.27*

Syrup 7.36 12 18.55±3.15

Compared with the control group *P<0.05,

The experimental results prove that Chaiyin Oral Liquid has a significant inhibitory effect on acute exudative inflammation at an equivalent dose (3.68g/kg).

Experimental example 5. Promoting effect on non-specific immunity of mice: 56 mice of 8-20g, half and half ♂♀, administered in groups as in 1.1.1, administered continuously for 8 days, 60 minutes after the last administration, administered via tail vein 20% Chinese ink 0.1ml/10g, injection, 2, 17min after injection, take blood 10ul from the bulbar venous plexus, add 2ml of 0.1% Na ₂ CO ₃ solution, at 640nm, 722 grating spectrophotometer For the OD value of the specimen, calculate the K value of the mouse carbon particle clearance rate according to the formula LogOD1-LogOD2/t2-t1. The results are shown in the table below.

       Table 12 Effects on the carbon clearance rate of mice

Group Dose g/kg Number of mice Clearance index K value (×10 ^-3 )

Control 14 2.40±0.94

Oral solution 3.68 14 4.76±1.32Δ**

Oral solution 7.36 14 5.27±1.86Δ**

Syrup 7.3 14 3.48±1.06*

Compared with the control group *P<0.05**P<0.001, compared with the syrup group ΔP<0.05

The experimental results confirmed that Chaiyin Oral Liquid can significantly promote the phagocytosis of reticulocytes, the non-specific immunity of mice, and its effect is significantly better than cold cough syrup at the same dose.

The following embodiments all can realize the effect of above-mentioned experimental example:

Example 1:

Bupleurum 50g Honeysuckle 40g Scutellaria baicalensis 30g Pueraria 25g

Nepeta 25g Artemisia annua 40g Forsythia 40g Bitter almond 25g

Mint 40g Houttuynia cordata 40g Bellflower 25g

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Artemisia annua, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect the distillate for redistillation , collect the heavy distilled liquid, store it in another container, combine the medicine dregs with the three flavors of Scutellaria baicalensis, Pueraria root, and Campanulaceae, add water to decoct three times, each time for 2 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add Ethanol, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate, add the above red distillate, and finally go through conventional procedures to make tablets directly or by adding pharmaceutically acceptable excipients.

Example 2:

Bupleurum 150g Honeysuckle 110g Scutellaria baicalensis 90g Pueraria 75g

Nepeta 75g Artemisia annua 110g Forsythia 110g Bitter almond 75g

Mint 110g Houttuynia cordata 110g Bellflower 75g

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Artemisia annua, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect the distillate for redistillation , collect the heavy distilled liquid, store it in another container, combine the medicine dregs with the three flavors of Scutellaria baicalensis, Pueraria root, and Campanulaceae, add water to decoct three times, each time for 2 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add Ethanol, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate, add the above red distillate, and finally make granules directly or by adding pharmaceutically acceptable excipients through conventional procedures.

Example 3:

Bupleurum 80g Honeysuckle 60g Scutellaria baicalensis 50g Pueraria 40g

Nepeta 40g Artemisia annua 60g Forsythia 60 Bitter almond 40g

Mint 60g Houttuynia cordata 60g Bellflower 40g

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Artemisia annua, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect the distillate for redistillation , collect the heavy distilled liquid, store it in another container, combine the medicine dregs with the three flavors of Scutellaria baicalensis, Pueraria root, and Campanulaceae, add water to decoct three times, each time for 2 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add Ethanol, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate, add the above red distilled liquid, and finally make capsules directly or by adding pharmaceutically acceptable excipients through conventional procedures.

Example 4:

Bupleurum 120g Honeysuckle 90g Scutellaria baicalensis 70g Pueraria 60g

Nepeta 60g Artemisia annua 90g Forsythia 90g Bitter almond 60g

Mint 90g Houttuynia cordata 90g Bellflower 60g

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Artemisia annua, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect the distillate for redistillation , collect the heavy distilled liquid, store it in another container, combine the medicine dregs with the three flavors of Scutellaria baicalensis, Pueraria root, and Campanulaceae, add water to decoct three times, each time for 2 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add Ethanol, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate, add the above red distilled liquid, and finally make capsules directly or by adding pharmaceutically acceptable excipients through conventional procedures.

Example 5:

Bupleurum 100g Honeysuckle 75g Scutellaria baicalensis 60g Pueraria 50g

Nepeta 50g Artemisia annua 75g Forsythia 75g Bitter almond 50g

Mint 75g Houttuynia cordata 75g Bellflower 50g

For the above eleven flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Qinghao, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect 2875ml of distillate for re- Distill, collect 575ml of the heavy distilled liquid, store in another container, combine the medicinal dregs with the three flavors of baicalin, kudzu root, and bellflower, add water to decoct three times, each time for 2 hours, combine the decoction with the above-mentioned distilled medicinal liquid, filter, and concentrate the filtrate When the relative density is 1.11-1.14 at 50°C, add ethanol until the alcohol content is 60%, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate until the relative density is 1.13-1.15 at 50°C, add the above weight Distilled liquid, stevioside 2g and sodium benzoate 3g, adjust the total amount of liquid medicine to 1000ml with distilled water, stir well, refrigerate for 24 hours, filter, adjust the pH value of the liquid medicine to 5.5-7.5 with sodium hydroxide solution, fill , and sterilized to obtain an oral liquid preparation. Each bottle is filled with 20ml, which is equivalent to 14.7g of crude drug, and it is taken orally, 20ml each time, 3 times a day.

Embodiment 6:

Bupleurum 100g Honeysuckle 75g Scutellaria baicalensis 60g Pueraria 50g

Nepeta 50g Artemisia annua 75g Forsythia 75g Bitter almond 50g

Mint 75g Houttuynia cordata 75g

For the above ten flavors, take bitter almonds and crush them into coarse particles, mix them with seven flavors such as Bupleurum, honeysuckle, Qinghao, forsythia, Nepeta, mint, and Houttuynia cordata, soak in water for 1 hour, heat and distill, collect the distillate for redistillation, Collect the heavy distilled liquid and store it in another container. Combine the medicinal dregs with Scutellaria baicalensis and Pueraria lobata root, add water and decoct three times for 2 hours each time, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add ethanol, Stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, concentrate, add the above red distillate, and finally make granules directly or by adding pharmaceutically acceptable excipients through conventional procedures.

Embodiment 7:

Bupleurum 100g Honeysuckle 75g Scutellaria baicalensis 60g Pueraria 50g

Nepeta 50g Artemisia annua 75g Forsythia 75g

The above seven flavors, Bupleurum, Honeysuckle, Artemisia annua, Forsythia, Nepeta and other five flavors, add water to soak for 1 hour, heat and distill, collect the distillate for redistillation, collect the double distillate, and store it in a separate container. Combine the two flavors, add water and decoct three times, 2 hours each time, combine the decoction with the above-mentioned distilled medicinal liquid, filter, concentrate the filtrate, add ethanol, stir well, refrigerate for 24 hours, take the supernatant to recover ethanol under reduced pressure, Concentrate, add the above red distillate, and finally make soft capsules directly or by adding pharmaceutically acceptable excipients through conventional procedures.

Claims (8)

1, a kind of pharmaceutical composition for the treatment of anemopyretic cold is characterized in that this pharmaceutical composition made by following raw material medicaments:

Radix Bupleuri 50-150 weight portion Flos Lonicerae 40-110 weight portion Radix Scutellariae 30-90 weight portion

Radix Puerariae 25-75 weight portion Herba Schizonepetae 25-75 weight portion Herba Artemisiae Annuae 40-110 weight portion

Fructus Forsythiae 40-110 weight portion.

2, a kind of pharmaceutical composition for the treatment of anemopyretic cold is characterized in that this pharmaceutical composition made by following method:

Take by weighing following crude drug:

Radix Bupleuri 50-150 weight portion Flos Lonicerae 40-110 weight portion Radix Scutellariae 30-90 weight portion

Radix Puerariae 25-75 weight portion Herba Schizonepetae 25-75 weight portion Herba Artemisiae Annuae 40-110 weight portion

Fructus Forsythiae 40-110 weight portion Semen Armeniacae Amarum 25-75 weight portion Herba Menthae 40-110 weight portion

Herba Houttuyniae 40-110 weight portion Radix Platycodonis 25-75 weight portion;

Get Semen Armeniacae Amarum and be broken for coarse granule, with Radix Bupleuri, Flos Lonicerae, Herba Artemisiae Annuae, Fructus Forsythiae, Herba Schizonepetae, Herba Menthae, Herba Houttuyniae seven flavors were soaked 1 hour, added thermal distillation, collecting distillate distills again, collect re-distilled liquid, device is preserved in addition, medicinal residues and Radix Scutellariae, Radix Puerariae, Radix Platycodonis three flavors merge, decoct with water three times, each 2 hours, the medicinal liquid after decocting liquid and the above-mentioned distillation merged, and filtered, filtrate concentrates, add ethanol, stir evenly, cold preservation 24 hours, get the supernatant decompression recycling ethanol, concentrate, add above-mentioned re-distilled liquid, excipient direct through conventional operation at last or that adding is pharmaceutically accepted is made the dosage form of clinical acceptance.

3, pharmaceutical composition as claimed in claim 2 is characterized in that the crude drug of this pharmaceutical composition is:

Radix Bupleuri 80-120 weight portion Flos Lonicerae 60-90 weight portion Radix Scutellariae 50-70 weight portion

Radix Puerariae 40-60 weight portion Herba Schizonepetae 40-60 weight portion Herba Artemisiae Annuae 60-90 weight portion

Fructus Forsythiae 60-90 weight portion Radix Platycodonis 40-60 weight portion Semen Armeniacae Amarum 40-60 weight portion

Herba Menthae 60-90 weight portion Herba Houttuyniae 60-90 weight portion.

4, pharmaceutical composition as claimed in claim 3 is characterized in that the crude drug of this pharmaceutical composition is:

Radix Bupleuri 100 weight portion Flos Loniceraes 75 weight portion Radix Scutellariaes 60 weight portions

Radix Puerariae 50 weight portion Herba Schizonepetae 50 weight portion Herba Artemisiae Annuaes 75 weight portions

Fructus Forsythiae 75 weight portion Radix Platycodoniss 50 weight portion Semen Armeniacae Amarums 50 weight portions

Herba Menthae 75 weight portion Herba Houttuyniae 75 weight portions.

5, pharmaceutical composition as claimed in claim 1 is characterized in that adding excipient and makes the dosage form of clinical acceptance in this pharmaceutical composition.

6,, it is characterized in that said excipient is one or more in the middle of solvent, coloring agent, correctives, dispersant, binding agent, thickening agent, lubricant, diluent, disintegrating agent, the antiseptic according to the said pharmaceutical composition of claim 5.

7, pharmaceutical composition as claimed in claim 5, the dosage form that it is characterized in that this pharmaceutical composition are pill, tablet, granule, oral liquid, capsule, unguentum, Emulsion, masticatory, injection or middle medicine film.

8, the application of pharmaceutical composition as claimed in claim 1 in the medicine of preparation treatment anemopyretic cold.