CN117017834A - Flos Caryophylli and cortex Cinnamomi essential oil composition, and its preparation method and application - Google Patents

Abstract

The invention belongs to the technical field of oral care, and particularly relates to a flos caryophylli and cortex cinnamomi essential oil composition, and a preparation method and application thereof. The composition is prepared by mixing flos caryophylli essential oil and cinnamon bark essential oil according to the volume ratio of 1:1-4, and the two produce synergistic effects, can be used as an oral antibacterial agent and an anticaries agent, and has inhibition effects on growth, acid production, biofilm formation and adhesion of streptococcus mutans, and the essential oil composition has potential for preventing dental caries caused by the streptococcus mutans.

Description

A kind of lilac flower and cinnamon bark essential oil composition and its preparation method and application

Technical field

The invention belongs to the technical field of oral care, and specifically relates to a lilac and cinnamon bark essential oil composition and its preparation method and application.

Background technique

Caries is a disease in which the hard tissues of the teeth are chronically destroyed under the influence of multiple factors, mainly bacteria. The main manifestations are tooth demineralization, staining, and the formation of cavities.

The occurrence of dental caries is mainly related to the expression of virulence factors of Streptococcus mutans, its acid production and biofilm formation. Streptococcus mutans (S.mutans) is the main cariogenic bacterium in the oral cavity, also known as dental plaque. It is a variety of biofilms that form on the hard surfaces of teeth. Streptococcus mutans can create a favorable living space for other acidogenic and acidic species by forming rich exopolysaccharides (EPS) and a low pH environment.

Streptococcus mutans (S. mutans) initially adheres to the tooth surface, producing an insoluble glucan layer. Glucans are synthesized by glycosyltransferases (Gtfs) and contribute to the formation of plaque matrix polysaccharides, thereby accelerating the maturation of dental plaque. Streptococcus mutans also has the ability to metabolize carbohydrates in food and release organic acids such as lactic acid as by-products. The released organic acids can lower the pH of plaque and dissolve tooth enamel. The development of dental caries is mainly related to the content of carbohydrates in the diet and the frequency of consumption. Sucrose is the most cariogenic carbohydrate because it is the major metabolite of Streptococcus mutans. Streptococcus mutans utilizes the production of acidic metabolites of carbohydrates, causing acidic damage and demineralization, removal of mineral substances, leading to dental caries. This sucrose-dependent mechanism is also based on Gtfs and involves some virulence factors related to cariogenicity, such as glucan-binding proteins (Gbps). gbpB, which encodes the surface-associated glucan-binding protein (Gbp), is also essential for bacterial adhesion, as this protein mediates the interaction between the cell surface and glucan. In addition, Streptococcus mutans expresses the spaP gene, which helps the adhesion of Streptococcus mutans. Therefore, reducing the number of cariogenic bacteria, reducing acid synthesis and preventing plaque biofilm formation have become the most effective methods to prevent and treat caries. strategy.

At present, the prevention and treatment methods for dental caries usually include laser caries prevention, immune caries prevention, pit and fissure sealant treatment, drug prevention and daily care prevention and treatment. Among them, daily care prevention and treatment combined with drug prevention and treatment are the most commonly used. The currently widely used technology is to add anti-caries compounds/compositions to oral care agents. Fluoride toothpaste is one of the commonly used methods of maintaining oral hygiene. It has many advantages. High clinical efficacy, but the use of fluoride-containing chemicals can cause changes in intestinal and oral flora, and even lead to tooth staining, vomiting and oral cancer. Triclosan (TCS) is a commonly used antibacterial/antifungal agent found in some toothpastes and mouthwashes. Although an effective antibacterial agent, the widespread use of triclosan in everyday products will lead to antibiotic resistance. Ethanol is one of the main active ingredients in most mouthwashes on the market, with some products containing ethanol concentrations as high as 27%. Using such high concentrations of ethanol in these products can cause irritation and dryness in the mouth. Therefore, there is an urgent need to develop a new antibacterial and anti-caries oral care composition.

Chinese invention patent application No. 01129070.6 discloses a composition for treating toothache caused by dental caries. It uses asarum, Zanthoxylum bungeanum, Ligusticum chuanxiong, mint, lead and camphor as raw materials to make a medicine for treating toothache caused by dental caries. It contains lead and is suitable for long-term use. Can cause side effects such as lead poisoning. Chinese invention patent application 201010173234.8 discloses a multifunctional mouthwash that uses disinfectants, anti-caries agents, detergents, fragrances, sweeteners, stabilizers and deionized water to form a mouthwash that can inhibit and remove oral bacteria. A mouthwash that effectively prevents tooth decay and removes bad breath. The anti-caries agent is one or more of potassium fluoride, sodium fluoride, and sodium monofluorophosphate. These substances all contain fluorine, and long-term use may cause fluorosis. There are dangers of side effects, and the first two substances are corrosive, so this mouthwash still has certain safety risks.

Contents of the invention

In view of the shortcomings of the existing technology, the technical problem actually solved by the present invention is to provide a lilac and cinnamon bark essential oil composition and its preparation method and application. The composition has good antibacterial activity and has good dental caries treatment. Effective and fragrant, without any toxic or side effects.

The properties of plant essential oils have huge advantages in antibacterial and anti-biofilm. At the same time, they are safe and have no side effects, which just meets the requirements of oral care products. Essential oils can simultaneously have anti-inflammatory, analgesic, antibacterial, hemostatic and anti-pathogenic microbial effects, and are very effective in protecting the health of teeth and oral cavity.

The invention provides a composite essential oil composition, which includes lilac essential oil and cinnamon bark essential oil.

Preferably, the volume ratio of the lilac essential oil and the cinnamon bark essential oil is 1:0.8-10.

Further preferably, the volume ratio of the lilac essential oil and cinnamon bark essential oil is 1:1:1-4.

Preferably, in terms of mass percentage, the active ingredients of the lilac essential oil include 72%-73% eugenol and 16%-18% caryophyllene, and further preferably 72.88% eugenol and 16.98% caryophyllene.

Preferably, in terms of mass percentage, the active ingredients of the cinnamon bark essential oil include (E)-cinnamic aldehyde 55%-65% and cinnamyl acetate 9%-11%, and further preferably (E)-cinnamic aldehyde 60.98% and Cinnamyl acetate 10.95%.

Preferably, the lilac essential oil and cinnamon bark essential oil are recovered through hydrodistillation.

The present invention also relates to a preparation method of a composition, which is obtained by mixing the lilac essential oil and the cinnamon bark essential oil.

The invention also relates to the application of the composition in the preparation of drugs or oral care products for inhibiting oral bacteria.

Preferably, the oral bacteria include Streptococcus mutans and the composition is capable of treating dental caries.

Preferably, the drug or product inhibits the growth, acid production, biofilm formation and adhesion ability of Streptococcus mutans.

Compared with the existing technology, the beneficial effects of the present invention are:

(1) Clove essential oil and cinnamon bark essential oil have a synergistic effect when mixed;

(2) The composition in this technical solution can be added to oral products (such as toothpaste, mouthwash, tooth powder, etc.) and has a significant inhibitory effect on the oral pathogenic bacteria Streptococcus mutans (S.mutans). It has inhibitory effects on its growth, acid production, biofilm formation and adhesion, and the essential oil composition has the potential to be used to prevent dental caries caused by Streptococcus mutans;

(3) Essential oil is a mixture of a variety of volatile aromatic components. Compared with other extracts, it has a small molecular weight, is fat-soluble, and is highly volatile. It can quickly penetrate or destroy the bacterial phospholipid bilayer membrane and enter the interior of cells to exert its effects. Fast; the compound essential oil provided by this technical solution has obvious synergistic effects compared with single essential oils.

Description of the drawings

Figure 1 is a diagram of the inhibitory zone diameter effect of the essential oils of Examples 1-3 and Comparative Examples 1-4 on Streptococcus mutans;

Figure 2 is a time-sterilization curve of the essential oil of Example 1 against Streptococcus mutans;

Figure 3 is a diagram of the influence of the essential oil of Example 1 on Streptococcus mutans biofilm;

Figure 4 is a diagram showing the effect of the essential oil of Example 1 on the expression of Streptococcus mutans virulence factors vicR and relA;

Figure 5 is a diagram showing the effect of the essential oil of Example 1 on the expression of Streptococcus mutans virulence factors gtfB and gbpB;

Figure 6 is a diagram showing the effect of the essential oil of Example 1 on the expression of Streptococcus mutans virulence factors gtfD and gtfC;

Figure 7 is a diagram showing the effect of the essential oil of Example 1 on the expression of virulence factors spaP and brpA of Streptococcus mutans.

Detailed ways

The technical solutions in the embodiments of the present invention are further clearly described. The described embodiments are only part of the present invention and are used to explain the present invention, but are not used to limit the present invention. Therefore, other people skilled in the art can do so without creative work. Other embodiments obtained below all belong to the protection scope of the present invention.

Lilac (Syzygium aromaticum) essential oil was purchased from Shanghai Poly Co., Ltd., China, product number: PEC1601;

Cinnamon bark (Cinnamomum zeylanicum) essential oil was purchased from Shanghai Poly Co., Ltd., China, product number: PEC2201;

Streptococcus mutans was purchased from the Microbial Culture Collection Center of the Chinese Academy of Sciences, preservation number: ATCC700610;

Brain heart infusion broth (BHI) medium, BR, was purchased from OXOID;

Nutrient agar, BR, was purchased from Beijing Aoboxing Biotechnology Co., Ltd.

Example 1

The essential oil composition is calculated by volume: 1 part of lilac essential oil and 4 parts of cinnamon bark essential oil.

Example 2

The essential oil composition, calculated by volume, is: 2 parts of lilac essential oil and 3 parts of cinnamon bark essential oil.

Example 3

The essential oil composition is calculated by volume: 1 part of lilac essential oil and 1 part of cinnamon bark essential oil.

Comparative example 1

The essential oil composition is calculated by volume: 3 parts of lilac essential oil and 2 parts of cinnamon bark essential oil.

Comparative example 2

The essential oil composition is calculated by volume: 4 parts of lilac essential oil and 1 part of cinnamon bark essential oil.

Comparative example 3

Single ingredient: lilac essential oil.

Comparative example 4

Single Ingredient: Cinnamon Bark Essential Oil.

Test Example 1 Antibacterial Activity Test

1. Medium preparation

Preparation of liquid culture medium: Weigh 37g of Brain Heart Infusion Broth (BHI) culture base into a beaker, add distilled water to dissolve it, and adjust the volume to 1L, and finally distribute it into appropriate Erlenmeyer flasks with seals Membrane sealing; sterilize at 120°C for 20 minutes before use.

Preparation of solid culture medium: Weigh 37g of Brain Heart Infusion Broth (BHI) culture medium and 15g of agar into a beaker, add an appropriate amount of distilled water and heat to dissolve, stir evenly, adjust the pH value, and distribute into appropriate sizes. Put it in an Erlenmeyer flask and seal it with a sealing film; after sterilization at 120°C for 20 minutes, wait until the temperature drops below 50°C, then distribute it into sterile petri dishes (perform this operation on a clean workbench) and cool it for later use.

2. Preparation of bacterial suspension

Pick the bacterial liquid and streak it on nutrient agar for 24 hours, then select a single colony with good growth, inoculate it into the liquid medium, and culture it under anaerobic conditions at 37°C for 16 hours. Then, after stepwise dilution, the plate is coated and the bacterial colonies are counted, the concentration of the bacterial solution is calculated, and a suspension of 10 ⁶ -10 ⁷ CFU/mL is made.

3. Measurement of inhibition zone diameter

The diameter of the inhibition zone was measured using the filter paper method. Take 200 μL of the prepared bacterial suspension (2×10 ⁶ CFU/mL) and spread it evenly on the BHI agar medium. Then, put three sterile filter paper pieces with a diameter of 6 mm in each dish, and drop 3 μL of essential oil. In the center of the filter paper, use an equal volume of sterile water as a blank control, incubate at 37°C for 24 hours, and measure the diameter of the inhibition zone. Each essential oil is measured three times and the average value is taken. The criterion for the inhibition zone test is the antibacterial index calculated from the positive control chlorhexidine.

4. Statistical processing

All experiments were performed in triplicate. The experimental data were analyzed using SPSS19.0 software, and one-way analysis of variance (ANOVA) was used to compare the overall means of each group. Mapping is performed using Origin 8.5.1.

5. Antibacterial activity test against Streptococcus mutans

The filter paper method was used to detect the antibacterial activity of the essential oils of Examples 1-3 and Comparative Examples 1-4. The measurement results of the diameters of all inhibition zones are shown in Figure 1. The results showed that lilac essential oil and cinnamon bark essential oil had a synergistic antibacterial effect when compounded at volume ratios of 1:4, 2:3 and 1:1. The diameter of the inhibition zone of the compound essential oil in the present invention is larger than the diameter of the inhibition zone of the single essential oil, and the compound essential oil with a volume ratio of 4:1 and 3:2 of lilac essential oil and cinnamon bark essential oil shows good synergy, and specifically inhibits The bacterial effect data are shown in Table 1.

Table 1 Antibacterial activity test results

Test Example 2 Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)

The MIC of the compound essential oil in Example 1 against Streptococcus mutans was determined using the broth microdilution method. Since the essential oil is insoluble in water, it is mixed with a 1:1 (v/v) DMSO:Tween20 and BHI liquid medium (which acts as an emulsifier for the essential oil and promotes contact with bacteria). Add the essential oil solution of known concentration to the bacterial suspension of 1×10 ⁶ CFU/mL, and culture it on a shaking table at 37°C for 24 hours. The positive control group was 1% chlorhexidine, with 1:1 (v/v) DMSO:Tween20 and BHI liquid medium. The negative control group was DMSO:Tween20 and BHI liquid culture medium containing 0.1%, 1:1 (v/v). The concentration of essential oil corresponding to the ability to inhibit the growth of bacteria in the culture medium by naked eye observation is the minimum inhibitory concentration, and the growth of bacteria is expressed as turbidity.

After determining the MIC, take 100 μL each of the bacterial suspension with the minimum inhibitory concentration and the bacterial suspension above the minimum inhibitory concentration, spread it on a BHI agar petri dish, and incubate anaerobically at 37°C for 24 hours. The concentration of essential oil corresponding to the bacterial liquid in which no colonies are grown in the solid medium is the MBC value. The test results are shown in Table 2.

Table 2 Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oils

Test Example 3 Time-killing effect test on Streptococcus mutans

The agar dilution method was used to determine the minimum bactericidal concentration of lilac + cinnamon bark essential oil to be 0.625 μL/mL, and the time-bactericidal curve of the essential oil composition in Example 1 was measured to reflect the bacterial killing effect of MBC concentrations at different times. Streptococcus mutans (1×10 ⁶ CFU/mL) was cultured in BHI medium, and the mixed bacterial solution was placed in a shaking incubator at 37°C and 180 rpm at a concentration of MBC. Every other day, take the bacterial suspension and spread it on the solid BHI agar medium, incubate for 24 hours, and record the changes in the number of single colonies in the dish. The experiment was repeated three times and the average value was calculated. Draw the sterilization curve with the action time as the abscissa and the natural logarithm of the viable bacterial count as the ordinate.

The bacterial suspension without adding essential oil composition was used as the negative control group, the bacterial suspension adding 1% chlorhexidine was used as the positive control group, and the 1:4 ratio of lilac + cinnamon bark essential oil composition in Example 1 of the present invention was used as Experimental group; the time-sterilization curve of the essential oil composition with a concentration of 0.625 μL/mL against Streptococcus mutans within 10 hours is shown in Figure 2. Throughout the culture period, the number of Streptococcus mutans in the blank control group showed a gradual increasing trend. The compound essential oil of lilac + cinnamon bark in a ratio of 1:4 in Example 1 maintains a sustained killing effect on Streptococcus mutans. As time goes by, more bacteria are killed, and the bactericidal effect is slightly enhanced as time goes by.

Test Example 4 Antibacterial Mechanism Test on Streptococcus mutans

1. Effect on acid production ability of Streptococcus mutans

Taking the composition of Example 1 as the research object, the effect of MIC, 1/2MIC, 1/4MIC and 1/8MIC concentration on the acid production ability of Streptococcus mutans was measured. Essential oils of different concentrations were added to 2mL containing 1% glucose. The concentration is 1×10 ⁷ CFU/mL in the Streptococcus mutans suspension. First, use a pH meter to measure the initial pH1. After incubating anaerobically for 24 hours in a 37°C incubator, measure pH2 again. The difference between the two pH values, △pH, is the effect on acid production. Three repeated experiments were performed for each group. The results are as shown in the table. 3 shown. There were 3 parallel controls for each concentration.

Table 3 Effects of different concentrations of compound essential oils on the acid-producing ability of Streptococcus mutans

test group Composition concentration pH1 pH2 △pH 1 MIC 7.04±0.00 6.99±0.02 0.05±0.02 2 1/2MIC 7.04±0.00 7.01±0.03 0.03±0.03 3 1/4MIC 7.04±0.00 7.01±0.01 0.03±0.01 4 1/8MIC 7.04±0.00 5.39±0.06 1.65±0.06 control group 0μL/mL 7.04±0.00 4.75±0.03 2.29±0.03

By measuring changes in pH value, we determined whether different concentrations of compound essential oil inhibited the acid production of Streptococcus mutans at a concentration of 1% sucrose. After 24 hours of culture, the pH value of the blank control group without compound essential oil decreased from 7.04 to 4.75. The compositions of lilac essential oil + cinnamon bark essential oil at concentrations of MIC, 1/2MIC, 1/4MIC and 1/8MIC all had a significant inhibitory effect on the decrease in pH value.

2. Effect on Streptococcus mutans biofilm

(1) Safranin staining method to observe the morphology of biofilms

Biofilm formation was measured by safranin staining, observed with the naked eye, and photographed. In a 35mm polystyrene petri dish, the 1:4 ratio of lilac + cinnamon bark compound essential oil in Example 1 was used as the research object, and different concentrations of essential oils were added to BHI broth containing 1% sucrose. The broth was inoculated with Streptococcus mutans seed culture (2.0×10 ⁷ CFU/mL) and cultured for 24 hours. After incubation, remove the supernatant and rinse the Petri dish with distilled water.

(2) Crystal violet staining to observe the impact of biofilm formation

Mix the bacterial suspension and the prepared medicinal solutions of different concentrations at a ratio of 1:1 (v/v) and inoculate 100 μL of each in a 96-well plate, with 5 duplicate wells of each concentration. After gently blowing with a sterile pipette to mix evenly, place the 96-well plate in a constant-temperature bacterial incubator for anaerobic cultivation at 37°C. BHI liquid medium inoculated with only bacteria but without essential oil was used as a control group. After 24 hours of culture, take out the 96-well plate, aspirate the mixture, rinse twice with sterile water, and stain with 0.1 mL of 0.4% crystal violet for 15 min. Rinse three times with sterile water, place 100 μL of 95% ethanol solution in each well, shake to decolorize, and measure the OD value at 540 nm with a UV spectrophotometer. The inhibition rate was calculated according to the formula, and the results are shown in Table 4.

Inhibition rate = (1-OD experimental group/OD control group)*100%

The effect of 1:4 ratio of lilac + cinnamon bark compound essential oil on Streptococcus mutans biofilm was determined at MIC, 1/2MIC, 1/4MIC and 1/8MIC concentrations. The biofilm formed by Streptococcus mutans on the surface of the petri dish was observed by safranin staining and crystal violet staining, as shown in Figure 3. The results showed that at MIC, the biofilm structure was destroyed, and surface bacteria died and fell off, reducing the activity of S. mutans biofilm in a concentration-dependent manner.

Table 4 Effects of different concentrations of compound essential oils on Streptococcus mutans biofilm

test group Composition concentration (μL/mL) OD value Inhibition rate(%) 1 MIC 0.26 86.41 2 1/2MIC 0.45 76.54 3 1/4MIC 0.56 70.48 4 1/8MIC 0.82 56.76 control group 0μL/mL 1.90 -

3. Effect on virulence factors of Streptococcus mutans

3.1 RNA extraction method

(1) Collect Streptococcus mutans that has grown to the logarithmic phase and prepare a Streptococcus mutans suspension with a concentration of 10 ⁵ CFU/mL;

(2) Add three different concentrations of essential oils of 1/8MIC, 1/4MIC and 1/2MIC of the compound essential oil of Example 1;

(3) Cultivate at 37°C for 24 hours and centrifuge at 5000r/min for 5 minutes;

(4) Wash with PBS, transfer the bacteria to an RNase-free centrifuge tube, centrifuge at 5000r/min for 5 minutes, and then carefully remove the supernatant;

(5) Grind and mash the sample in liquid nitrogen, add 1mL Transzol, and pipette evenly to completely disrupt the cells. After leaving it for 5 minutes, add chloroform to remove protein, let it stand for 5-10 minutes, and centrifuge at 10000g, 4°C. 15 minutes, then take the supernatant into a new RNase-free centrifuge tube, add an equal amount of ethanol, add it to the RNase-free column and pass through the column, centrifuge at 12000g, 4°C for 30 seconds, and discard the supernatant;

(6) Add 500 μL CB9 solution, centrifuge at 12000g for 1 min, repeat once;

(7) Add 500 μL WB9 solution, centrifuge at 12000g for 1 min, repeat once;

(8) Idle centrifuge for 2 minutes, 12000g;

(9) Place the RNase-free column into a new RNase-free centrifuge tube. After drying at room temperature for 10 minutes, add 20 μL RNase-free water and let stand for 2 minutes. Centrifuge at 12000g for 1 minute.

3.2 Reverse transcription

Table 5 shows the reverse transcription reaction system.

Table 5 Reverse transcription reaction system

After the addition of reagents is completed, place the centrifuge tube into the PCR machine at 37°C and react for 5 minutes.

Synthesis of cDNA: After adding reagents according to Table 6, put the centrifuge tube into a PCR machine for reaction at 37°C for 15 minutes; 50°C for 5 minutes; and 98°C for 5 minutes.

Table 6 Reverse transcription reaction system

3.3 Real-time fluorescence quantification

Synthesis and design of primers: Obtain the full-length mRNA sequence of each target gene from GenBank, and use the software Primer 5.0 to design primers. The primer sequences of the primers used for the target gene and the internal reference gene 16S rRNA are shown in 7.

Table 7 Primers used for the target gene and primer sequences of the internal reference gene 16S rRNA

RT-PCR: According to Table 8, add various substances in the table to 200 μL centrifuge tubes, mix evenly and put them into the PCR machine. Settings: 95°C pre-denaturation for 2 minutes; 95°C deformation for 30 seconds; 57°C annealing for 30 seconds. The reaction was carried out under the reaction conditions: 72°C extension for 30 seconds; ×38 cycles; 72°C extension for 2 minutes.

Table 8 Fluorescence quantitative PCR reaction system

Note: Fluorescent dyes include 10× buffer, Green 1, Taq enzyme, dNTPs.

Taking the compound essential oil of Example 1 as the research object, the essential oil composition with sub-minimum inhibitory concentration (1/8-1/2MIC) and the negative control group (chlorhexidine 1%) were used to treat Streptococcus mutans and cultured for 24 hours, using real-time Fluorescence quantitative PCR method was used to detect the effect of compound essential oil on the expression of virulence factors of Streptococcus mutans. As shown in Figure 4-7, after treatment with compound essential oil, the mRNA expression of brpA, gbpB, gtfB, gtfC, gtfD, vicR, spaP and relA genes was significantly reduced.

The results show that when the concentration of the compound essential oil in Example 1 is 1/2 MIC, the number of bacteria decreases, the colony effect between bacteria weakens, and the expression of virulence factors decreases. Inhibits biofilm formation by inhibiting gtfB, gtfC, gtfD and vicR, and inhibits acid production by inhibiting the expression of brpA and relA. spaP and gbpB play key roles in the adhesion of Streptococcus mutans to the tooth surface.

The invention provides a composite essential oil composition of clove essential oil and cinnamon bark essential oil. The final preferred ratio of lilac essential oil and cinnamon bark essential oil is 1:1-4. The specific implementation ratios are 1:4, 2:3, and 1:1 respectively, and the optimal ratio is 1:4. The active ingredients of lilac essential oil are 72.88% eugenol and 16.98% caryophyllene. The active ingredients of cinnamon bark essential oil include (E)-cinnamaldehyde 60.98% and cinnamyl acetate 10.95%. The lilac essential oil and cinnamon bark essential oil are recovered through water distillation.

The composition of lilac essential oil and cinnamon bark essential oil shows good antibacterial effect on Streptococcus mutans, and the two have obvious synergistic effect; the essential oil composition in the present invention has a good effect on the growth, acid production ability and biofilm formation of Streptococcus mutans. Both the ability and adhesion ability have obvious inhibitory effects, so the essential oil composition of the present invention has the potential to be used to prevent dental caries caused by Streptococcus mutans.

The above detailed description is a specific description of one of the possible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. All equivalent implementations or changes that do not depart from the present invention should be included in the present invention. within the scope of technical solutions.

Claims (10)

1. The composite essential oil composition is characterized in that the raw materials of the composition comprise flos caryophylli essential oil and cortex cinnamomi essential oil.

2. The essential oil composition according to claim 1, wherein the volume ratio of the lilac flower essential oil to the cinnamon bark essential oil is 1:0.8-10.

3. The essential oil composition according to claim 1, wherein the volume ratio of the lilac flower essential oil to the cinnamon bark essential oil is 1:1-4.

4. The essential oil composition according to claim 1, wherein the effective components of the lilac flower essential oil comprise, in mass percent, 72% -73% of eugenol and 16% -18% of caryophyllene.

5. The essential oil composition according to claim 1, wherein the effective components of the cinnamon bark essential oil comprise (E) -cinnamaldehyde 55% -65% and cinnamyl acetate 9% -11% by mass.

6. The essential oil composition of claim 1, wherein the lilac flower essential oil and the cinnamon bark essential oil are both recovered by water distillation.

7. A method for preparing the composition according to any one of claims 1 to 6, wherein the lilac flower essential oil and the cinnamon bark essential oil are mixed.

8. Use of a composition according to any one of claims 1 to 6 or a composition obtainable by a process according to claim 7 for the preparation of a medicament or oral care product for inhibiting oral bacteria.

9. The use according to claim 8, wherein the oral bacteria comprise streptococcus mutans and the composition is capable of treating dental caries.

10. The use according to claim 8, wherein the medicament or care product is capable of inhibiting the growth, acid production, biofilm formation and/or adhesion capacity of streptococcus mutans.