CN114323783B - Sampling method, sampling assembly and sample analyzer - Google Patents
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- CN114323783B CN114323783B CN202011061707.5A CN202011061707A CN114323783B CN 114323783 B CN114323783 B CN 114323783B CN 202011061707 A CN202011061707 A CN 202011061707A CN 114323783 B CN114323783 B CN 114323783B
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Abstract
The application relates to the technical field of medical equipment, and particularly discloses a sampling method, a sampling assembly and a sample analyzer. The sampling method comprises the following steps: before a sampling needle filled with first liquid leaves a first mixing container, driving the sampling needle to absorb the first mixing liquid in the first mixing container so that a part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and the second liquid; the sampling needle is driven to leave the first mixing container. By the above mode, when the spherical liquid beads drop into or are mixed into the first uniform liquid in the first uniform container due to the surface tension being destroyed in the working process of the sample analyzer or when the sampling needle leaves the first uniform container, the type and the concentration of the first uniform liquid in the first uniform container are hardly changed, so that the influence of the spherical liquid beads on an analysis result can be avoided, and the accuracy of the analysis result is improved.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to a sampling method, a sampling assembly and a sample analyzer.
Background
A sample analyzer is an instrument for analyzing data of biological samples such as blood and urine.
In the long-term research and development process, the inventor finds that in the actual operation process of the instrument in the prior art, because the surface tension exists at the liquid outlet of the sampling needle, the sample liquid forms a spherical liquid bead at the liquid outlet, and the spherical liquid bead stays at the liquid outlet for a long time. Because the sample analyzer must have conditions such as mechanical vibration or pipeline extrusion in the working process, the surface tension can be damaged, and the spherical liquid beads drop to the reaction cup to cause the sampling amount or sampling concentration change, so that serious errors exist in the subsequent analysis results.
Disclosure of Invention
Based on the above, the application provides a sampling method, a sampling assembly and a sample analyzer, which can improve the accuracy of analysis results.
In order to solve the technical problems, the application adopts a technical scheme that: there is provided a sampling method comprising: before a sampling needle filled with first liquid leaves a first mixing container, driving the sampling needle to absorb the first mixing liquid in the first mixing container so that a part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and the second liquid; the sampling needle is driven to leave the first mixing container.
In order to solve the technical problems, the application adopts another technical scheme that: there is provided a sampling assembly for performing the sampling method as described above, the sampling assembly comprising: a sampling needle for aspirating at least a first liquid; a first mixing vessel for mixing the first liquid and the second liquid to form a first mixed liquid; the first driving unit is used for driving the sampling needle to absorb the first uniform mixing liquid in the first uniform mixing container so that a part of the cavity of the sampling needle, which is close to one end of the first uniform mixing container, is filled with the first uniform mixing liquid; and the second driving unit is connected with the sampling needle and is used for driving the sampling needle to move in the horizontal direction and/or the vertical direction.
In order to solve the technical problems, the application adopts another technical scheme that: there is provided a sample analyzer comprising a sampling assembly as described above and a measurement assembly connected to the sampling assembly.
The beneficial effects of the application are as follows: in contrast to the prior art, the sampling needle is driven to absorb the first uniform liquid in the first uniform mixing container before the sampling needle filled with the first liquid leaves the first uniform mixing container, so that the part of the cavity of the sampling needle, which is close to one end of the first uniform mixing container, is filled with the first uniform liquid. In this way, if the spherical liquid beads formed at the liquid outlet due to the influence of the surface tension are spherical liquid beads of the first uniform mixing liquid, the spherical liquid beads are dropped or mixed into the first uniform mixing liquid in the first uniform mixing container due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle is separated from the first uniform mixing container, the type and concentration of the first uniform mixing liquid in the first uniform mixing container are hardly changed, and therefore, the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
FIG. 1 is a schematic flow chart of a first embodiment of the sampling method of the present application;
FIG. 2 is a schematic view of the structure of the sampling needle of the present application;
FIG. 3 is a flow chart of a second embodiment of the sampling method of the present application;
FIG. 4 is a flow chart of a third embodiment of the sampling method of the present application;
FIG. 5 is a flow chart of a fourth embodiment of the sampling method of the present application;
FIG. 6 is a flow chart of a fifth embodiment of the sampling method of the present application;
FIG. 7 is a flow chart of a sixth embodiment of the sampling method of the present application;
FIG. 8 is a schematic diagram of the structure of a first embodiment of the sampling assembly of the present application;
FIG. 9 is a schematic diagram of a second embodiment of a sampling assembly of the present application;
fig. 10 is a schematic view of the structure of a third embodiment of the sampling assembly of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
An embodiment of the present application proposes a sampling method, please refer to fig. 1 and fig. 8-10, which includes:
s11: before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, the sampling needle 11 is driven to suck the first mixing liquid in the first mixing container 12, so that a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid.
The first uniform mixing liquid at least comprises a first liquid and a second liquid, and the first uniform mixing liquid is liquid to be detected or configured. Optionally, the volume of the first liquid in the first homogenized liquid is substantially smaller than the volume of the second liquid in the first homogenized liquid, e.g. the volume ratio of the first liquid to the second liquid may be 1:100-1:500, e.g. 1:100, 1:200, 1:300, 1:400, 1:450, 1:500.
Optionally, the first liquid may be a sample liquid such as blood, urine, etc., and the first liquid may also be a reagent or a diluent to be disposed; the second liquid may be a reagent or diluent for pre-reaction with the sample liquid prior to detection. When the first liquid is a sample liquid and the second liquid is a reagent for pre-reacting with the sample liquid before detection, the first mixing container 12 is a cuvette. It will be appreciated that the reagents described above for pre-reacting with the sample fluid prior to entry into the measurement assembly may be a variety of reagents, such as hemolysis agents, buffers or staining agents, and are not limited in this regard.
Referring to fig. 2, the inner cavity of the sampling needle 11 is an L-shaped hollow cavity, and the L-shaped hollow cavity includes a first horizontal subcavity 101 and a first vertical subcavity 102 that are communicated, where the first horizontal subcavity 101 is close to the first mixing container 12. After the sampling needle 11 sucks the first mixing liquid in the first mixing container 12, the first mixing liquid is at least filled into the corner of the L-shaped hollow cavity (i.e., the connection between the first horizontal subchamber 101 and the first vertical subchamber 102). In this way, when the surface tension is broken and the first uniform liquid in the first horizontal subcavity 101 drops into the first uniform mixing container 12, the first liquid in the first vertical subcavity 102 is not mixed into the first uniform mixing container 12. It should be noted that this embodiment is applicable to any sampling needle, and the structure of the sampling needle 11 in fig. 2 is merely an example and is not limited thereto.
Further, since the inner diameter of the sampling needle 11 is only 0.2 to 0.8mm, even if the surface tension is broken, only a small amount of the first mixing liquid is dropped from the liquid outlet of the sampling needle 11 or mixed into the first mixing container 12. The volume of the first uniform liquid sucked by the sampling needle 11 can be set according to the user's requirement, and is not limited herein.
S12: the sampling needle 11 is driven out of the first mixing container 12.
Specifically, after the portion of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid, the sampling needle 11 is driven to leave the first mixing container 12. The spherical liquid beads formed at the liquid outlet of the sampling needle 11 due to the influence of the surface tension are spherical liquid beads of the first uniform mixing liquid, and if the spherical liquid beads drop into or mix into the first uniform mixing liquid in the first uniform mixing container 12 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 leaves the first uniform mixing container 12, the type and concentration of the first uniform mixing liquid in the first uniform mixing container 12 are hardly changed.
In contrast to the prior art, the present application drives the sampling needle 11 to suck the first mixing liquid in the first mixing container 12 before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, so that a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid. In this way, if the spherical liquid beads formed at the liquid outlet due to the influence of the surface tension are spherical liquid beads of the first uniform mixing liquid, the spherical liquid beads are dropped or mixed into the first uniform mixing liquid in the first uniform mixing container 12 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 is separated from the first uniform mixing container 12, the type and concentration of the first uniform mixing liquid in the first uniform mixing container 12 are hardly changed, and therefore, the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
In some embodiments, referring to fig. 3 and fig. 8-10, before S11, the method further includes:
s13: the sampling needle 11 is driven into the first mixing container 12, and the liquid outlet of the sampling needle 11 is positioned below the liquid surface of the first mixing container 12 filled with the second liquid.
Specifically, before step S13, the sampling needle 11 sucks the first liquid in a test tube (not shown), and after the sampling needle 11 sucks the first liquid in the test tube, the outer wall of the sampling needle 11 is cleaned. Simultaneously, the second liquid is injected into the first mixing container 12. The sampling needle 11 is then extended into the second liquid of the first mixing container 12.
S14: the sampling needle 11 is driven to inject the first liquid into the first mixing container 12 to form the first mixed liquid.
Specifically, the sampling needle 11 may inject the first liquid several times below the liquid surface of the first mixing container 12. In addition, a mixing mechanism (e.g., a stirring magnet or a mechanical stirring rod) (not shown) may be installed in the first mixing container 12 to improve the mixing efficiency of the first liquid and the second liquid.
In some embodiments, referring to fig. 4 and fig. 8-10, when there are at least two mixing containers in the sample analyzer, after step S12, the method further includes:
s15: the sampling needle 11 filled with the first liquid is driven into the second mixing container 13, and the liquid outlet of the sampling needle 11 is positioned below the liquid surface of the second mixing container 13 filled with the third liquid.
Specifically, before step S15, the third liquid is injected into the second mixing container 13. The sampling needle 11 is then extended into the third liquid of the second mixing container 13.
S16: the sampling needle 11 is driven to inject the first liquid into the second mixing container 13 to form a second mixing liquid, wherein the second mixing liquid at least comprises the first liquid and the third liquid.
Specifically, the sampling needle 11 may inject the first liquid several times below the liquid surface of the second mixing container 13. In addition, a mixing mechanism (e.g., a stirring magnet or a mechanical stirring rod) (not shown) may be installed in the second mixing container 13 to improve the mixing efficiency of the first liquid and the third liquid.
S17: before the sampling needle 11 filled with the first liquid leaves the second mixing container 13, the sampling needle 11 is driven to suck the second mixing liquid in the second mixing container 13, so that the part of the cavity of the sampling needle 11 near one end of the second mixing container 13 is filled with the second mixing liquid.
The second uniform mixing liquid at least comprises a first liquid and a third liquid, and the first uniform mixing liquid is liquid to be detected or configured. Optionally, the volume of the first liquid in the second homogenized liquid is much smaller than the volume of the third liquid in the second homogenized liquid, e.g. the volume ratio of the first liquid to the third liquid may be 1:100-1:500, e.g. 1:100, 1:200, 1:300, 1:400, 1:450, 1:500.
Optionally, the first liquid may be a sample liquid such as blood, urine, etc., and the first liquid may also be a reagent or a diluent to be disposed; the third liquid may be a reagent or diluent for pre-reaction with the sample liquid prior to detection. When the first liquid is a sample liquid and the third liquid is a reagent for pre-reacting with the sample liquid before detection, the second mixing container 13 is a cuvette. It will be appreciated that the reagent that is pre-reacted with the sample fluid prior to detection may be a variety of reagents, such as a hemolyzing agent or a buffer, and is not limited thereto.
It should be noted that, referring to fig. 2, the inner cavity of the sampling needle 11 is an L-shaped hollow cavity, and the L-shaped hollow cavity includes a first horizontal subcavity 101 and a first vertical subcavity 102 that are communicated, where the first horizontal subcavity 101 is close to the second mixing container 13. After the sampling needle 11 sucks the second mixing liquid in the second mixing container 13, the second mixing liquid is at least filled into the corner of the L-shaped hollow cavity (i.e., the connection between the first horizontal subchamber 101 and the first vertical subchamber 102). In this way, when the surface tension is broken and the second uniform mixing liquid in the first horizontal subcavity 101 drops into the second uniform mixing container 13, the first liquid in the first vertical subcavity 102 is not mixed into the second uniform mixing container 13.
S18: the sampling needle 11 is driven out of the second mixing container 13.
Specifically, after the second mixing liquid is filled in a part of the cavity of the sampling needle 11 near one end of the second mixing container 13, the sampling needle 11 is driven to leave the second mixing container 13. The spherical liquid beads formed at the liquid outlet of the sampling needle 11 due to the influence of the surface tension are spherical liquid beads of the second uniform mixing liquid, and if the spherical liquid beads drop into or mix into the second uniform mixing liquid in the second uniform mixing container 13 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 leaves the second uniform mixing container 13, the type and concentration of the second uniform mixing liquid in the second uniform mixing container 13 are hardly changed.
In the above manner, before the sampling needle 11 filled with the first liquid leaves the second mixing container 13, the sampling needle 11 is driven to suck the second mixing liquid in the second mixing container 13, so that a part of the cavity of the sampling needle 11, which is close to one end of the second mixing container 13, is filled with the second mixing liquid. In this way, if the spherical liquid beads formed at the liquid outlet due to the influence of the surface tension are spherical liquid beads of the second uniform mixing liquid, the spherical liquid beads are dropped or mixed into the second uniform mixing liquid in the second uniform mixing container 13 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 is separated from the second uniform mixing container 13, the type and concentration of the second uniform mixing liquid in the second uniform mixing container 13 are hardly changed, and therefore, the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
Referring to fig. 5 and fig. 8-10, in some embodiments, before step S15, the method further includes:
s19: the first uniform liquid and part of the first liquid in the sampling needle 11 are discharged.
Since the composition and/or concentration of the first homogenized liquid is different from that of the second homogenized liquid, the first homogenized liquid in the sampling needle 11 needs to be discharged before step S15, and furthermore, since the first liquid is contained in the sampling needle 11, the inner wall of the sampling needle 11 cannot be cleaned, and the purpose of flushing the first transverse subchamber 101 is achieved by discharging part of the first liquid.
Wherein the first mixing liquid in the sampling needle 11 and part of the first liquid may be discharged through the first mixing container 12 or a swab (not shown).
After step S19, the process advances to step S20 or S21.
S20: the outer wall of the sampling needle 11 is cleaned with a cleaning liquid.
Since the liquid outlet of the sampling needle 11 is located below the liquid surface of the first mixing container 12 containing the second liquid in step S13, the second liquid and the first mixed liquid are adhered to the outer wall of the sampling needle 11, and the outer wall of the sampling needle 11 needs to be cleaned by using a cleaning liquid in order to avoid contamination of the second mixed liquid.
S21: the outer surface of the sampling needle 11 is air-dried.
It will be appreciated that the outer surface of the sampling needle 11 is air-dried to eliminate the effect of the cleaning fluid or other agent on the second mixed liquid.
If there are multiple mixing containers in the sample analyzer to separate samples, the steps S15-S21 may be repeated to achieve the purpose of multiple sample separation.
Referring to fig. 6-7 and 8-10, in some embodiments, after step S12 or S18, the method further includes:
s22: the outer wall and the inner wall of the sampling needle 11 are cleaned by cleaning liquid.
Specifically, after the sampling needle 11 leaves the first mixing container 12 or the second mixing container 13, if the sampling needle 11 is not separating, the outer wall and the inner wall of the sampling needle 11 are cleaned with a cleaning liquid.
An embodiment of the present application proposes a sampling assembly 10 for performing the sampling method of the above embodiment, referring to fig. 8, the sampling assembly 10 includes: a sampling needle 11, a first mixing container 12, a first drive unit 14 and a second drive unit (not shown).
The sampling needle 11 is used for sucking at least a first liquid. It should be noted that, referring to fig. 2, the inner cavity of the sampling needle 11 is an L-shaped hollow cavity, and the L-shaped hollow cavity includes a first horizontal subcavity 101 and a first vertical subcavity 102 that are communicated, and the first horizontal subcavity 101 is close to the first mixing container 12. After the sampling needle 11 sucks the first mixing liquid in the first mixing container 12, the first mixing liquid is at least filled into the corner of the L-shaped hollow cavity (i.e., the connection between the first horizontal subchamber 101 and the first vertical subchamber 102). In this way, when the surface tension is broken and the first uniform liquid in the first horizontal subcavity 101 drops into the first uniform mixing container 12, the first liquid in the first vertical subcavity 102 is not mixed into the first uniform mixing container 12. It should be noted that this embodiment is applicable to any sampling needle, and the structure of the sampling needle 11 in fig. 2 is merely an example and is not limited thereto.
The first mixing vessel 12 is used to mix the first liquid and the second liquid to form a first mixed liquid.
The first drive unit 14 is used to provide driving power for the liquid in the sampling needle.
Specifically, the first driving unit 14 includes: the first voltage supply device 141 and the control circuit 143. The first pressure supply device 141 is connected to the sampling needle 11 through the fourth electromagnetic valve 174, and the control circuit 143 is electrically connected to the first pressure supply device 141 and controls the first pressure supply device 141. The control circuit 143 is configured to control the first pressure supply device 141 to provide driving power for the liquid in the sampling needle 11 before the sampling needle 11 containing the first liquid leaves the first mixing container 12, so that the sampling needle 11 can suck the first mixing liquid in the first mixing container 12, so that a portion of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid. The first pressure supply device 141 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited herein.
The sampling needle 11 is fixed to a second drive unit for moving the sampling needle 11 filled with the first liquid in a horizontal direction and/or a vertical direction so that the sampling needle 11 enters or leaves the first drive unit 14.
Unlike the prior art, the control circuit 143 of the present application is used for controlling the first pressure supply device 141 to pull the first pressure supply device 141 to drive the sampling needle 11 to suck the first mixing liquid in the first mixing container 12 before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, so that a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid. In this way, if the spherical liquid beads formed at the liquid outlet due to the influence of the surface tension are spherical liquid beads of the first uniform mixing liquid, the spherical liquid beads are dropped or mixed into the first uniform mixing liquid in the first uniform mixing container 12 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 is separated from the first uniform mixing container 12, the type and concentration of the first uniform mixing liquid in the first uniform mixing container 12 are hardly changed, and therefore, the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
Referring to fig. 9, in some embodiments, the sampling assembly 10 further comprises: a second mixing container 13.
The second mixing container 13 is used for mixing the first liquid and the third liquid to form a second mixed liquid.
The second driving unit is further used for driving the sampling needle 11 filled with the first liquid to enter the second mixing container 13, and enabling the liquid outlet of the sampling needle 11 to be located below the liquid level of the second mixing container 13.
The control circuit 143 is configured to control the first pressure supply device 141 to provide driving power for the liquid in the sampling needle 11 before the sampling needle 11 containing the first liquid leaves the second mixing container 13, so that the sampling needle 11 can suck the second mixing liquid in the second mixing container 13, so that a portion of the cavity of the sampling needle 11 near one end of the second mixing container 13 is filled with the second mixing liquid. The first pressure supply device 141 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited herein.
The second driving unit is also used for driving the sampling needle 11 to leave the second mixing container 13.
The sampling assembly 10 further comprises: a reservoir for storing a diluent or other reagents required for the reaction, the first mixing vessel 12 and/or the second mixing vessel 13 being controllably connected to the reservoir.
With continued reference to fig. 9, the reservoir may be a reactant reservoir 15. The reaction liquid pool 15 is used for storing the reaction liquid. In some embodiments, the first liquid and the second liquid may be the same type and concentration of the reaction liquid, where the reaction liquid tank 15 is connected to the first mixing container 12 through the first electromagnetic valve 171, the reaction liquid tank 15 is connected to the second mixing container 13 through the second electromagnetic valve 172, and the reaction liquid tank 15 is connected to the cleaning unit 18 through the third electromagnetic valve 173, so that the reaction liquid is injected into the first mixing container 12, the second mixing container 13, and the cleaning unit 18, respectively.
Further, with continued reference to fig. 9, the first mixing container 12 and the second mixing container 13 are respectively connected to the second pressure supplying device 121, and the second pressure supplying device 121 is configured to supply driving power to the liquid in the first mixing container 12 and the second mixing container 13, so as to inject the reaction liquid in the reaction liquid tank 15 into the first mixing container 12 and the second mixing container 13 when the first electromagnetic valve 171 and the second electromagnetic valve 172 are opened. The second pressure supply device 121 may be an air pump, a liquid pump, a syringe, a negative pressure tank, etc., and is not limited herein.
Referring to fig. 10, in certain embodiments, the sampling assembly 10 further comprises: a cleaning unit 18. The cleaning unit 18 is used for cleaning the outer wall of the sampling needle 11 with a cleaning liquid. Further, the cleaning unit 18 is in communication with a third pressure supply device 19 via a sixth electromagnetic valve 176, and the third pressure supply device 19 is configured to provide driving power for the cleaning liquid in the cleaning unit 18.
With continued reference to fig. 10, in some embodiments, the first pressure supply device 141 may be a syringe, the liquid container may be a diluent tank 16, the diluent tank 16 is used for storing diluent, and the diluent tank 16 is connected to a liquid inlet of the syringe through a fifth electromagnetic valve 175 to inject the diluent into the syringe, so as to clean the inner wall of the sampling needle 11 through the syringe.
The embodiment of the application provides a sample analyzer, which can be used for analyzing biological samples, wherein the biological samples can be blood, urine and the like. The sample analyzer may further include a drive assembly, a measurement assembly, a waste treatment assembly, and a controller in addition to the sampling assembly 10 of the above embodiments. The drive assembly is used to drive various flow paths (including gas and liquid paths) in the sample analyzer. The measuring assembly is connected with the sampling assembly and is used for detecting the first uniform mixing liquid to form detection information. The waste liquid treatment assembly is used for collecting and discharging waste liquid in the sample analyzer. The controller is used for controlling the working flow of the sample analyzer and processing the detection information to obtain a result.
In contrast to the prior art, the present application drives the sampling needle 11 to suck the first mixing liquid in the first mixing container 12 before the sampling needle 11 filled with the first liquid leaves the first mixing container 12, so that a part of the cavity of the sampling needle 11 near one end of the first mixing container 12 is filled with the first mixing liquid. In this way, if the spherical liquid beads formed at the liquid outlet due to the influence of the surface tension are spherical liquid beads of the first uniform mixing liquid, the spherical liquid beads are dropped or mixed into the first uniform mixing liquid in the first uniform mixing container 12 due to the surface tension being destroyed during the operation of the sample analyzer or when the sampling needle 11 is separated from the first uniform mixing container 12, the type and concentration of the first uniform mixing liquid in the first uniform mixing container 12 are hardly changed, and therefore, the influence of the spherical liquid beads on the analysis result can be avoided, and the accuracy of the analysis result can be improved.
In the description of the present application, it should be noted that the azimuth or positional relationship indicated by the terms "upper", "lower", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element in question must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application or directly or indirectly applied to other related technical fields are included in the scope of the present application.
Claims (9)
1. A sampling method, the sampling method comprising:
driving a sampling needle filled with first liquid into a first mixing container, wherein a liquid outlet of the sampling needle is positioned below the liquid level of the first mixing container filled with second liquid;
driving the sampling needle to inject the first liquid into the first mixing container so as to form first mixing liquid;
before a sampling needle filled with the first liquid leaves the first mixing container, driving the sampling needle to absorb the first mixing liquid in the first mixing container so that a part of a cavity of the sampling needle, which is close to one end of the first mixing container, is filled with the first mixing liquid, wherein the first mixing liquid at least comprises the first liquid and the second liquid;
the sampling needle is driven to leave the first mixing container.
2. The sampling method of claim 1, wherein after the driving the sampling needle out of the first blending container, the method further comprises:
discharging the first uniform mixed liquid and part of the first liquid in the sampling needle;
driving a sampling needle filled with the first liquid into a second mixing container, wherein a liquid outlet of the sampling needle is positioned below the liquid level of the second mixing container filled with the third liquid;
driving the sampling needle to inject the first liquid into the second mixing container so as to form second mixing liquid, wherein the second mixing liquid at least comprises the first liquid and the third liquid;
before a sampling needle filled with the first liquid leaves the second mixing container, driving the sampling needle to absorb the second mixing liquid in the second mixing container so that a part of a cavity of the sampling needle, which is close to one end of the second mixing container, is filled with the second mixing liquid;
and driving the sampling needle to leave the second mixing container.
3. The method of sampling according to claim 2, wherein,
the ratio of the volume of the first liquid in the first uniform mixing liquid to the volume of the second liquid in the first uniform mixing liquid is 1:100-1:500;
the ratio of the volume of the first liquid in the second uniform mixing liquid to the volume of the third liquid in the second uniform mixing liquid is 1:100-1:500.
4. The sampling method of claim 2, wherein after the draining of the first homogenized liquid and a portion of the first liquid within the sampling needle, the sampling method further comprises:
and cleaning the outer wall of the sampling needle by adopting cleaning liquid, or air-drying the outer wall of the sampling needle.
5. The sampling method of claim 2, wherein after driving the sampling needle away from the first blending container or the second blending container, the sampling method further comprises:
and cleaning the outer wall and the inner wall of the sampling needle by adopting cleaning liquid.
6. A sampling assembly for performing the method of any one of claims 1-5, the sampling assembly comprising:
a sampling needle for aspirating at least a first liquid;
a first mixing vessel for mixing the first liquid and the second liquid to form a first mixed liquid;
the first driving unit is used for driving the sampling needle to absorb the first uniform mixing liquid in the first uniform mixing container so that a part of the cavity of the sampling needle, which is close to one end of the first uniform mixing container, is filled with the first uniform mixing liquid;
and the second driving unit is connected with the sampling needle and is used for driving the sampling needle to move in the horizontal direction and/or the vertical direction.
7. The sampling assembly of claim 6, further comprising:
a second mixing vessel for mixing the first liquid and the third liquid to form a second mixed liquid;
the second driving unit is used for driving the sampling needle filled with the first liquid to enter or leave the first driving unit, and is also used for driving the sampling needle filled with the first liquid to enter the second mixing container, and the liquid outlet of the sampling needle is positioned below the liquid level of the second mixing container;
the first driving unit is also used for driving the sampling needle to absorb the second uniform mixing liquid in the second uniform mixing container, so that the second uniform mixing liquid is filled in a part of the cavity of the sampling needle, which is close to one end of the second uniform mixing container.
8. The sampling assembly of claim 7, further comprising:
and the liquid storage container is used for storing a diluent or reagents required by other reactions, and the first mixing container and/or the second mixing container are controllably connected with the liquid storage container.
9. A sample analyzer comprising a sampling assembly according to any one of claims 6 to 8 and a measurement assembly coupled to the sampling assembly.
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| CN202011061707.5A CN114323783B (en) | 2020-09-30 | 2020-09-30 | Sampling method, sampling assembly and sample analyzer |
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| CN202011061707.5A CN114323783B (en) | 2020-09-30 | 2020-09-30 | Sampling method, sampling assembly and sample analyzer |
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| CN114323783B true CN114323783B (en) | 2023-11-24 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CA1087070A (en) * | 1978-02-23 | 1980-10-07 | David R. Friswell | Sample injection with automatic cleaning of sampling conduit |
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| CN114323783A (en) | 2022-04-12 |
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