CN102870977A - Wheat middling-corn type compound feed for lactating sow and preparation method of compound feed - Google Patents

Abstract

The invention discloses a secondary meal-corn type compound feed for lactating sows, which contains the following components in parts by weight: corn 9-12; secondary meal 50-60; biscuit powder 3.5-4.2; wheat bran 5-7; 10-14 soybean meal; 1.8-2.5 chocolate; 1.3-1.8 fish meal; 1.2-1.7 wheat protein; , one or more of Bacillus. The characteristics of the present invention are: the price of the secondary powder-corn type ration is lower than that of the corn-soybean meal type ration on the market, and a certain amount of beneficial microorganisms are added to the premix, which can solve the problem that the secondary powder type ration is not easily digested by pigs Contradictory, it can also promote lactation of lactating sows, increase feed intake and shorten the estrus interval after weaning.

Description

A kind of secondary flour-corn type compound feed for lactating sows and its preparation method

technical field

The invention belongs to the field of feed, and in particular relates to a lactating sow meal-corn type compound feed and a preparation method thereof.

Background technique

Most of China's traditional pig farming industry uses corn-soybean meal-based rations. Although my country is a large planting country, due to its large population, corn and soybeans still need to be imported in large quantities from foreign countries. In recent years, the problem of human and livestock competing for food has become increasingly prominent, so a diet that can meet the growth of livestock and poultry and is reasonably priced needs to be developed urgently. Secondary flour is one of the by-products obtained after grinding various flours with wheat seeds as a raw material, and it has a poor taste when it is eaten by people. Secondary flour is cheap and easy to obtain, rich in nutrition, and its protein level is 6% higher than that of corn. After adding xylanase, its digestibility level is equivalent to that of corn, and its cost performance is significantly higher than that of corn. There is a flour mill near the farm to meet the ration preparation.

Sows consume a lot of feed. Sows in the peak lactation period can eat up to 10 kg of feed a day. It is the most important production factor in large-scale pig production. The quality of feeding sows is directly related to the farm. economic benefits. The lactation, re-estrus after weaning and utilization years of lactating sows are all directly related to the feed intake and nutritional level during lactation.

Contents of the invention

In order to solve the above-mentioned problems, the present invention provides a kind of secondary meal-corn type compound feed for lactating sows and a preparation method thereof.

The compound feed provided by the invention contains the following components in parts by weight: 9-12 parts of corn; 50-60 parts of secondary powder; 3.5-4.2 parts of biscuit powder; 5-7 parts of wheat bran; ; Fish meal 1.3-1.8; Wheat protein 1.2-1.7; Bean flour 3.8-4.4; Compound microbial agent 0.05-0.1, wherein, the compound microbial agent includes one or more of Enterococcus, yeast, and Bacillus .

In one embodiment of the present invention, described compound feed also can contain the premix I of 2.3-2.7 parts by weight, and described premix I comprises:

Rice husk powder 14-17%, zeolite powder 15-17%, vitamin E 0.38-0.45%, xylanase 0.75-0.85%, phytase 1.0-1.4%, 50% carnitine 0.35-0.45%, hydrogen phosphate Calcium 55-70%, multidimensional 3.8-4.5%. Among them, the multidimensional includes: one or more of VA, VD3, VK3, VB1, VB2, VB6, VB12, VE50, nicotinamide, calcium pantothenate, 2% biotin, α-lipoic acid, and folic acid.

In another embodiment of the present invention, the compound feed can also contain 2.2-2.6 parts by weight of premix II, and the premix II includes: rice husk powder 4-5%, zeolite powder 4-5% , organic chromium 1.8-2.2%, stone powder 50-55%, 50% choline 5.5-7.0%, salt 12-16%, 98.5% lysine 6.6-7.2%, 98% threonine 1.0-1.4%, 99% %Valine 0.7-0.9%, 98%DL-Methionine 0.75-0.85%, Polymine 6.8-7.5%, Magnesium Sulfate 0.3-0.5%, Potassium Chloride 0.35-0.45%. Wherein, the composite minerals include one or more of copper sulfate pentahydrate, ferrous sulfate monohydrate, zinc sulfate monohydrate, manganese sulfate monohydrate, potassium iodide, sodium selenite, and cobalt chloride.

Wherein, the enterococcus is preferably Enterococcus faecalis and/or Enterococcus faecium, and the yeast is preferably Saccharomyces cerevisiae and/or Candida utilis, The bacillus is preferably Bacillus subtilis and/or Bacillus licheniformis.

Wherein, the Enterococcus faecalis is Enterococcus faecalis CGMCC No.5092, the Saccharomyces cerevisiae is Saccharomycescerevisiae CGMCC No.2388, and the Bacillus subtilis is Bacillus subtilis ) CGMCC No.4628, the Bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094.

Among them, the viable count of Enterococcus faecalis is not less than 1.0×10 ⁸ cfu/g, the viable count of Enterococcus faecium is not lower than 1.0×10 ⁸ cfu/g, and the viable count of Saccharomyces cerevisiae is not lower than 1.0×10 ⁷ cfu/g gram, the viable count of Candida utilis not less than 1.0×10 ⁷ cfu/g, the viable count of Bacillus subtilis not less than 1.0×10 ⁸ cfu/g, the viable count of Bacillus licheniformis not less than 1.0×10 ⁸ cfu/gram.

The present invention also provides a preparation method of the compound feed, which comprises weighing various raw materials by weight, putting them into a pulverizer for pulverization with a pulverizing force of 20-40 mesh, and then pulverizing them into a mixer for uniform mixing.

The price of the sub-flour type ration of the present invention is lower than that of the corn-soybean meal type ration on the market, and a certain amount of beneficial microorganisms are added. Compared with the common corn-soybean meal type ration, the feed of the present invention is used to feed lactating sows, It can increase the feed intake of lactating sows, promote the digestion of sows, increase the milk production, reduce constipation, and lay a good foundation for the future growth of piglets. production days.

The Bacillus subtilis used in the present invention is isolated by the applicant from traditional fermented soybean meal, and its biological characteristics are as follows: the surface of the colony is rough, opaque, dirty white, the colony is round, the edges are jagged, and Gram-positive Bacteria; spores are oval to columnar in shape, located in the center of the thallus or slightly off, and the thallus does not expand after spore formation. The Bacillus subtilis of the present invention is significantly different from the existing Bacillus subtilis in that it can tolerate pH 2.0, 1% pepsin artificial gastric juice, can tolerate 0.3% artificial bile salt, and can withstand a granulation temperature of 80°C , can inhibit pathogenic Escherichia coli K88, K99 and Staphylococcus aureus, has a strong ability to produce cellulase, and can degrade cellulose. The Bacillus subtilis of the present invention has been preserved on March 2, 2011 in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, referred to as CGMCC, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences, deposit number is CGMCC No.4628.

The Saccharomyces cerevisiae of the present invention has good resistance to gastric acid, bile salts, high temperature, and common antibiotics, and probiotic functions such as acid production, enzyme production, and inhibition of pathogenic bacteria. It was isolated and selected from the soil or feces, and was identified by the China Agricultural Microorganism Culture Collection Management Center, and was preserved in the General Microbiology Center of the China Microbiology Culture Collection Management Committee on March 3, 2008, referred to as CGMCC, address: Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, Institute of Microbiology, Chinese Academy of Sciences, the preservation number is CGMCC No.2388.

The Bacillus licheniformis of the present invention is obtained by the applicant from the intestinal tract of cattle, and is obtained through directional primary screening and re-screening. Its vegetative cells are rod-shaped, and its spores are oval or oblong, mesomorphic or subterminal, and the cysts are slightly enlarged. Solitary or in short chains with semicircular ends. Colonies can be formed within 12 to 16 hours. The colonies are round or irregular with hairy edges. The colonies are white, opaque, and wrinkle-free. Gram positive. The applicant has conducted tests on acid resistance, bile salt resistance, high temperature resistance, fermentation characteristics and antibacterial activity on this strain of Bacillus licheniformis. This strain of Bacillus licheniformis has strong tolerance to adverse environments, strong adhesion, and growth Fast, large biomass, good antibacterial activity and other characteristics. The Bacillus licheniformis of the present invention has been preserved by the applicant of the Bacillus licheniformis on July 26, 2011 in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, referred to as CGMCC, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No.5094.

Enterococcus faecalis of the present invention is isolated from pig large intestine by the applicant, and its biological characteristics are as follows: the colony is round, the surface is smooth, opaque, milky white, with smooth edges, and it is a Gram-positive bacterium; a single thalline is Spherical or elliptical. Enterococcus faecalis of the present invention is screened through artificial gastric juice, bile liquid, and heat resistance; and screened for enzyme production ability and antibacterial ability; % of artificial bile liquid, can withstand the granulation temperature of 85 ℃, and can also inhibit pathogenic Escherichia coli K88, K99 and Staphylococcus aureus. It has strong acid production and inhibits mold in soybean meal, cotton meal and corn straw Ability. The Enterococcus faecalis of the present invention has been deposited by the applicant on July 26, 2011 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, referred to as CGMCC, with the same address as above, and the preservation number is CGMCC No.5092.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

Example 1

Raw material composition of secondary meal-corn type compound feed for lactating sows:

100 kg of corn, 540 kg of flour, 40 kg of biscuit flour, 60 kg of wheat bran, 120 kg of soybean meal, 20 kg of chocolate, 40 kg of soybean flour, 15 kg of imported fish meal (65%), 15 kg of wheat protein (80%) , 25 kg of premix I, 25 kg of premix II, and 0.5 kg of compound microbial agent, totaling 1000.5 kg.

Premix I composition: calcium hydrogen phosphate 15 kg, xylanase 0.2 kg, phytase 0.3 kg, multivitamin 1 kg, vitamin E 0.1 kg, 50% carnitine 0.1 kg, rice husk powder 4.15 kg, zeolite powder 4.15 kg. Rice husk powder and zeolite powder are used as dilution carriers.

Composition of multivitamins: VA 99.01g, VD3 14.14g, VE50 388.97g, VK3 15.56g, VB1 5.66g, VB2 35.36g, VB6 21.22g, VB12 9.90g, Niacinamide 134.37g, Calcium pantothenate 77.79g, 2% biotin 141.44 grams, 28.29 grams of α-lipoic acid, and 28.29 grams of folic acid.

Premix II composition: 1.075 kg of rice husk powder, 1.075 kg of zeolite powder, 13 kg of stone powder, 1.5 kg of 50% choline, 1.75 kg of compound minerals, 3.5 kg of table salt, 0.5 kg of organic chromium, 1.7 kg of 98.5% lysine , 0.3 kg of 98% threonine, 0.2 kg of 99% valine, 0.2 kg of 98% DL-methionine, 0.1 kg of magnesium sulfate, and 0.1 kg of potassium chloride. Rice husk powder and zeolite powder are used as dilution carriers.

Compound mineral composition: 50 grams of copper sulfate pentahydrate, 100 grams of ferrous sulfate monohydrate, 400 grams of zinc sulfate monohydrate, 125 grams of manganese sulfate monohydrate, 100 grams of 1% potassium iodide, 150 grams of 1% sodium selenite, 1 % cobalt chloride 75 grams, stone powder 350 grams. Among them, stone powder provides 38% calcium.

Composition of complex microbial agent: Enterococcus faecalis (Enterococcus faecalis) CGMCCNo.5092 bacteria powder 100 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Enterococcus faecium (purchased from China Food Fermentation Industry Research Institute) bacterial powder 100 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Saccharomycescerevisiae (Saccharomycescerevisiae) CGMCC No.2388 bacterial powder 100 grams, the number of viable bacteria is not less than 1.0×10 ⁷ cfu/g, the product Candida utilis (purchased from China Food and Fermentation Industry Research Institute) bacterial powder 100 grams, the number of live bacteria is not less than 1.0×10 ⁷ cfu/g, Bacillus subtilis (Bacillus subtilis) CGMCC No.4628 bacterial powder 50 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram, and the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram for 50 grams of Bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094 bacteria powder.

Take various raw materials by weight, put them into a pulverizer for pulverization, and the pulverization force is 30 mesh, and then put them into a mixer to mix evenly.

Example 2

Raw material composition of secondary meal-corn type compound feed for lactating sows:

90 kg of corn, 575 kg of flour, 42 kg of biscuit powder, 50 kg of wheat bran, 100 kg of soybean meal, 25 kg of chocolate, 38 kg of soybean flour, 13 kg of imported fish meal (65%), 17 kg of wheat protein (80%) , 25 kg of premix I, 25 kg of premix II, 1 kg of compound microbial agent, a total of 1001 kg.

Premix I composition: calcium hydrogen phosphate 15 kg, xylanase 0.2 kg, phytase 0.3 kg, multivitamin 1 kg, vitamin E 0.1 kg, 50% carnitine 0.1 kg, rice husk powder 4.15 kg, zeolite powder 4.15 kg. Rice husk powder and zeolite powder are used as dilution carriers.

Composition of multivitamins: VA99.01g, VD3 14.14g, VE50 388.97g, VK3 15.56g, VB1 5.66g, VB2 35.36g, VB6 21.22g, VB12 9.90g, niacinamide 134.37g, calcium pantothenate 77.79g, 2% biological Vitamin 141.44 grams, α-lipoic acid 28.29 grams, folic acid 28.29 grams.

Premix II composition: 1.075 kg of rice husk powder, 1.075 kg of zeolite powder, 13 kg of stone powder, 1.5 kg of 50% choline, 1.75 kg of compound minerals, 3.5 kg of table salt, 0.5 kg of organic chromium, 1.7 kg of 98.5% lysine , 0.3 kg of 98% threonine, 0.2 kg of 99% valine, 0.2 kg of DL-methionine, 0.1 kg of magnesium sulfate, and 0.1 kg of potassium chloride. Rice husk powder and zeolite powder are used as dilution carriers.

Compound mineral composition: 50 grams of copper sulfate pentahydrate, 100 grams of ferrous sulfate monohydrate, 400 grams of zinc sulfate monohydrate, 125 grams of manganese sulfate monohydrate, 100 grams of 1% potassium iodide, 150 grams of 1% sodium selenite, 1 % cobalt chloride 75 grams, stone powder 350 grams. Among them, stone powder provides 38% calcium.

Composition of complex microbial agent: Enterococcus faecalis (Enterococcus faecalis) CGMCCNo.5092 bacteria powder 200 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Enterococcus faecium (purchased from China Food Fermentation Industry Research Institute) bacteria powder 200 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Saccharomycescerevisiae (Saccharomycescerevisiae) CGMCC No.2388 bacteria powder 200 grams, the number of viable bacteria is not less than 1.0×10 ⁷ cfu/g, the product Candida utilis (purchased from China Food and Fermentation Industry Research Institute) bacterial powder 200 grams, the number of live bacteria is not less than 1.0×10 ⁷ cfu/g, Bacillus subtilis (Bacillus subtilis) CGMCC No.4628 bacterial powder 100 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram, and the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram for 100 grams of Bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094 bacterial powder.

Take various raw materials by weight, put them into a pulverizer for pulverization, and the pulverization force is 30 mesh, and then put them into a mixer to mix evenly.

Example 3

Raw material composition of secondary meal-corn type compound feed for lactating sows:

120 kg of corn, 500 kg of flour, 42 kg of biscuit powder, 70 kg of wheat bran, 125 kg of soybean meal, 25 kg of chocolate, 38 kg of soybean flour, 13 kg of imported fish meal (65%), 17 kg of wheat protein (80%) , 25 kg of premix I, 25 kg of premix II, 0.8 kg of compound microbial agent, a total of 1000.8 kg.

Premix I composition: calcium hydrogen phosphate 15 kg, xylanase 0.2 kg, phytase 0.3 kg, multivitamin 1 kg, vitamin E 0.1 kg, 50% carnitine 0.1 kg, rice husk powder 4.15 kg, zeolite powder 4.15 kg. Rice husk powder and zeolite powder are used as dilution carriers.

Composition of multivitamins: VA99.01g, VD3 14.14g, VE50 388.97g, VK3 15.56g, VB1 5.66g, VB2 35.36g, VB6 21.22g, VB12 9.90g, niacinamide 134.37g, calcium pantothenate 77.79g, 2% biological Vitamin 141.44 grams, α-lipoic acid 28.29 grams, folic acid 28.29 grams.

Premix II composition: 1.075 kg of rice husk powder, 1.075 kg of zeolite powder, 13 kg of stone powder, 1.5 kg of 50% choline, 1.75 kg of compound minerals, 3.5 kg of table salt, 0.5 kg of organic chromium, 1.7 kg of 98.5% lysine , 0.3 kg of 98% threonine, 0.2 kg of 99% valine, 0.2 kg of DL-methionine, 0.1 kg of magnesium sulfate, and 0.1 kg of potassium chloride. Rice husk powder and zeolite powder are used as dilution carriers.

Compound mineral composition: 50 grams of copper sulfate pentahydrate, 100 grams of ferrous sulfate monohydrate, 400 grams of zinc sulfate monohydrate, 125 grams of manganese sulfate monohydrate, 100 grams of 1% potassium iodide, 150 grams of 1% sodium selenite, 1 % cobalt chloride 75 grams, stone powder 350 grams. Among them, stone powder provides 38% calcium.

Composition of complex microbial agent: Enterococcus faecalis (Enterococcus faecalis) CGMCCNo.5092 bacteria powder 150 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Enterococcus faecium (purchased from China Food Fermentation Industry Research Institute) bacteria powder 150 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/g, Saccharomycescerevisiae (Saccharomycescerevisiae) CGMCC No.2388 bacteria powder 200 grams, the number of viable bacteria is not less than 1.0×10 ⁷ cfu/g, the product Candida utilis (purchased from China Food and Fermentation Industry Research Institute) bacterial powder 200 grams, the number of live bacteria is not less than 1.0×10 ⁷ cfu/g, Bacillus subtilis (Bacillus subtilis) CGMCC No.4628 bacterial powder 50 grams, the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram, and the number of viable bacteria is not less than 1.0×10 ⁸ cfu/gram for 50 grams of Bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094 bacteria powder.

Take various raw materials by weight, put them into a pulverizer for pulverization, and the pulverization force is 30 mesh, and then put them into a mixer to mix evenly.

Test example 1

Experimental animals and diets:

Select 48 healthy late-pregnancy sows (Jia Dabai) with similar parity, age, and weight (about 107 days of gestation), and divide them into four treatments. 4. Feeding the rations of Example 1 to 3.

The formula of corn-soybean meal type diet is as follows: 620kg corn, 50kg bran, 120kg soybean meal, 130kg puffed soybean, 40kg 4% premix.

Test procedure:

The test began when pregnant sows entered the perinatal period (1 week before delivery to weaning), and the feed intake of sows throughout lactation was recorded. One week before delivery, the sows are transferred to the farrowing room, and the feeding amount is 1.8-2.5 kg/head/day. After the sows gave birth, the number of piglets born and their health status were recorded. Gradually increase the amount of feed 7 days after delivery, and reach ad libitum intake after 7 days, with an average daily intake of 7-10 kg per pig. Within 3 days after delivery, the cross-fostering method within the group was adopted to achieve the same litter size. Each sow had 8-10 piglets, and the adjusted piglet number and litter weight were recorded. During the lactation period, observe and record the diarrhea and death of piglets. Piglets are weaned at an average age of 21 days. After weaning, the number of piglets in each litter and the litter weight were recorded. The number of sows with constipation during lactation was recorded, and the estrus interval (days) of each sow was recorded after weaning.

test results:

Table 1 Statistical Table of Production Performance of Lactating Sows Feeding Different Types of Diets

Note: Daily gain of piglets = (average weight of piglets at weaning - average weight of piglets after adjustment)/days of lactation

Table 2 Statistical table of production performance of lactating sows fed with different types of diets to produce piglets

According to the test results in Table 1 and Table 2, the performance of sows fed the secondary meal-corn diet was not lower than that of the normal corn-soybean meal diet, and the feed intake of sows during lactation increased instead. Larger, this is because a certain amount of live microbial preparations are added to the diet to promote the digestion of sows, increase lactation, reduce constipation, and the weaning weight of piglets is higher, which lays a good foundation for the growth of piglets in the future. The interval between estrus after weaning of sows is also within the normal range, and the sows consuming the sub-milk diet have a tendency to shorten, which is also helpful to reduce the number of non-productive days of sows.

The performance of lactating sows fed the sub-meal-corn diet was better than that of the pigs fed the corn-soybean meal diet. The addition of beneficial microbial preparations in the diet can alleviate the indigestion caused by the sub-meal to a certain extent. Conditioned farms can feed pigs a conventional diet.

The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the technical principle of the present invention, some improvements and modifications can also be made. These improvements and modifications It should also be regarded as the protection scope of the present invention.

Claims (7)

1. milking sow time powder-corn type mixed feed is characterized in that, contains the component of following weight parts:

Corn 9-12;

Inferior powder 50-60;

Cracker meal 3.5-4.2;

Wheat bran 5-7;

Soybean Meal 10-14;

Chocolate 1.8-2.5;

Fish meal 1.3-1.8;

Wheat gluten 1.2-1.7;

Bean flour 3.8-4.4;

Complex micro organism fungicide 0.05-0.1,

Wherein, described complex micro organism fungicide comprises one or more in enterococcus, saccharomycete, the bacillus.

2. mixed feed as claimed in claim 1, it is characterized in that, the premix compound I that also contains the 2.3-2.7 weight portion, described premix compound I comprises: powdered rice hulls 14-17%, zeolite powder 15-17%, vitamin E 0.38-0.45%, zytase 0.75-0.85%, phytase 1.0-1.4%, 50% carnitine 0.35-0.45%, calcium monohydrogen phosphate 55-70%, B B-complex 3.8-4.5%, wherein B B-complex comprises: one or more in VA, VD3, VK3, VB1, VB2, VB6, VB12, VE50, niacinamide, calcium pantothenate, 2% biotin, alpha-lipoic acid, the folic acid.

3. mixed feed as claimed in claim 2, it is characterized in that, the premix compound II that also contains the 2.2-2.6 weight portion, described premix compound II comprises: powdered rice hulls 4-5%, zeolite powder 4-5%, Organic Chromium 1.8-2.2%, stone flour 50-55%, 50% choline 5.5-7.0%, salt 12-16%, 98.5% lysine 6.6-7.2%, 98% threonine 1.0-1.4%, 99% valine 0.7-0.9%, 98%DL-methionine 0.75-0.85%, composite mineral matter 6.8-7.5%, magnesium sulfate 0.3-0.5%, potassium chloride 0.35-0.45%, wherein, described composite mineral matter comprises cupric sulfate pentahydrate, ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate, KI, sodium selenite, in the cobalt chloride one or more.

4. such as each described mixed feed of claim 1 ~ 3, it is characterized in that, described enterococcus is enterococcus faecalis (Enterococcus faecalis) and/or VREF (Enterococcus faecium), described yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae) and/or candida utili (Candida utilis), and described bacillus is bacillus subtilis (Bacillus subtilis) and/or bacillus licheniformis (Bacillus licheniformis).

5. mixed feed as claimed in claim 4, it is characterized in that, described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) CGMCC No.5092, described saccharomyces cerevisiae is saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC No.2388, described bacillus subtilis is bacillus subtilis (Bacillus subtilis) CGMCC No.4628, and described bacillus licheniformis is bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094.

6. mixed feed as claimed in claim 5 is characterized in that, the enterococcus faecalis viable count is not less than 1.0 * 10 ⁸The cfu/ gram, the VREF viable count is not less than 1.0 * 10 ⁸The cfu/ gram, the saccharomyces cerevisiae viable count is not less than 1.0 * 10 ⁷The cfu/ gram, the candida utili viable count is not less than 1.0 * 10 ⁷The cfu/ gram, the bacillus subtilis viable count is not less than 1.0 * 10 ⁸The cfu/ gram, the bacillus licheniformis viable count is not less than 1.0 * 10 ⁸The cfu/ gram.

7. method for preparing each described mixed feed of claim 1 ~ 6, it drops into pulverizer and pulverizes for taking by weighing by weight various raw materials, and the pulverizing dynamics is the 20-40 order, drops into mixer again and mixes.