CN1094309A - Mineral absorption factor peptide calcium and preparation method of preparation thereof - Google Patents
Mineral absorption factor peptide calcium and preparation method of preparation thereof Download PDFInfo
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- CN1094309A CN1094309A CN 93111085 CN93111085A CN1094309A CN 1094309 A CN1094309 A CN 1094309A CN 93111085 CN93111085 CN 93111085 CN 93111085 A CN93111085 A CN 93111085A CN 1094309 A CN1094309 A CN 1094309A
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- calcium
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- Cosmetics (AREA)
Abstract
A peptide calcium of mineral absorption factor and its preparation method, in the field of bioengineering, the peptide calcium of mineral absorption factor is the mixture chelated with polypeptide, calcium and phosphoric acid, contain a large amount of amino acid existing in the form of active peptide, contain Ca 2-7%, P0.5-2%, have high solubility and stability under the extensive pH value, said substance and preparation have promoting the mineral substance, especially absorption of Ca, Fe, Zn, P, the absorption utilization rate of calcium can be up to 88%, the food conversion utilization rate can be up to 32%, have affinity to hydroxyapatite, calcium phosphate, it has effects of obviously resisting carious tooth and repairing the hole, have certain curative effect to osteoporosis, fracture, infantile chondropathy, said substance is mainly prepared by immobilized protease bioreactor, the cost of this method is low, can be industrialized continuously.
Description
The preparation method of a kind of mineral nitrogen absorption factor peptide calcium and preparation thereof, belong to bioengineering field, be particularly related to a kind of mineral nitrogen absorption factor peptide calcium and preparing preparation thereof, mineral nitrogen absorption factor peptide calcium, it is the mixture of polypeptide and calcium, phosphoric acid chelating, contain the aminoacid that exists with the small peptide form in a large number, extensively under the pH value extremely strong solubility is being arranged, therefore very easily be absorbed by the body, this material has special affinity to hydroxyapatite, so tangible osteoporosis and anti-caries effect are arranged, be the desirable material that human body replenishes calcium, phosphorus.
Be used for replenishing the various preparations of human body mineral at present, such as replenish the calcium, the phosphorus supplement preparation, be inorganic salt and organic salt mostly, commonly used have passive calcium-supplementing preparations such as calcium chloride, calcium phosphate, calcium gluconate, activated calcium, is subjected to the many-sided factor affecting of body, absorption rate is very low, market activated calcium preparation more salable, body absorption rate only are 59%, and Remineralization nutrition merely, do not possess the effect that replenishes other nutrition, also can influence body proteinic absorption.Another purpose that human body replenishes calcium phosphorus preparation is to prevent dental caries, at present the calcium phosphate that uses, calcium hydrogen phosphate because dissolubility is low, absorption difference, therefore anti-tooth demineralization ability a little less than, the effect of repairing cavity is very little.
The objective of the invention is for a kind of easily molten, easy absorption being provided and having the mineral nitrogen absorption factor peptide calcium that suppresses tooth demineralization effect more by force and the preparation method of preparation.
Mineral nitrogen absorption factor peptide calcium among the present invention, be the mixture of polypeptide and calcium and phosphoric acid chelating, contain phosphoserine calcium, phosphothreonine calcium structure in the peptide chain, this material is soluble in water in the scope of PH2-12, meet carbonic acid and do not precipitate, its component is (g/100g):
Calcium 1.5～7 phosphorus 0.5～2 total amino acid content 60～90 is wherein:
Asparatate 4～6.5 threonines 2～4 serines 3～18 proline 3s～5 glutamic acid, 15～22 alanine, 2～3 valines, 4～5 arginine, 1～3 cystine 0～micro-methionine 0.4～1 leucine 2～5 isoleucines 3～5 phenylalanines 1～3 lysine 1～5 histidine trace～1 glycine, 1～2 tyrosine trace～2.
With above-mentioned mineral nitrogen absorption factor peptide calcium, be aided with other adjuvant, can be made into various preparations, become health food or directly make health care medicine usefulness.
(1) fruity peptide calcium beverage, its component: (g%)
Peptide calcium 2～4 skim milks 5 CMC0.3 flavoring agents, an amount of fruit juice 90 of essence
(2) carbonated beverage, its component: (g%)
Peptide calcium 0.5～2 fruit juice, sugar, an amount of filling carbon dioxide of essence
(3) bone strengthening electuary, its component: (g%)
Peptide calcium 5～10 ferrous lactates 0.2 vitamin C is equipped with saccharic acid in right amount and is adjusted to 100
(4) peptide calcium tablet or capsule, its component (g%)
Peptide calcium 1g/ sheet, grain are equipped with sheet, the grain that magnesium stearate 0.4-0.6 starch 0.4-0.8 makes 2-3g
(5) chewing gum, its component: (g%)
Peptide calcium 2～5 mannitol, an amount of gum base 20-30 of sorbitol aspartame 30-60 flavoring agent are an amount of
(6) toothpaste, its component: (g%)
Peptide calcium 1～5 glycerol 30 phosphides 0.5 SDS0.5 starch 30
(7) toothpaste with fluoride, its component: (g%)
An amount of microcrystalline Cellulose of peptide calcium 2～5 sodium fluoride, 0.5 SDS, an amount of flavoring agent of starch are an amount of
(8) mouthful Su liquid, its component: (g%)
Peptide calcium 1～5 SDS0.5 hibitane 0.1 spice suitable quantity of water 90
(9) milk sugar-tablet, its component: (g%)
An amount of aspartame 70-90 of peptide calcium 1～5 milk powder 3 gelatin
Gene engineering method is generally adopted in the preparation of polypeptides matter at present, native protein acid, alkali hydrolysis method or enzyme hydrolysis method, and gene engineering research cost height, the acid and alkali hydrolysis method is wayward, the easy inactivation of its product.Utilize enzyme hydrolysis comparatively desirable, adopt solution enzyme direct hydrolysis usually, but industrial continuous production is comparatively difficult, the cost height especially uses expensive enzyme.
The preparation method of mineral nitrogen absorption factor peptide calcium comprises the steps: among the present invention
(1) with natural phosphoprotein, be suspended in the deionized water as yolk, frumentum, beans, newborn class, adopt isoelectric point precipitation to prepare pure phosphoprotein;
(2) pure phosphoprotein placed contain fixing bioreactor, adds 2-10 times of deionized water, make suspension, regulate PH6～11, in 30～60 ℃ of temperature ranges, react 0.5～8hr with glucose or fructose calcium salt than protease,
(3) the micropore filter filter pressing that is 0.45 μ with reacted liquid filter membrane, filtrate is the dual ultrafiltration of 500～1000 filter membranes through molecular cut off, with the trapped substance lyophilization, makes powdered substance-mineral nitrogen absorption factor peptide calcium.
Calcium is measured and is adopted network and titrimetry, phosphorus to measure the molybdic acid method that adopts among the present invention, and determined amino acid use automatic amino acid analyzer (Hitachi-835-50).
Mineral nitrogen absorption factor peptide calcium among the present invention, contain the aminoacid that exists with the small peptide form in a large number, extensively has high dissolution and stability under the pH value, very easily absorbed by body, in small intestinal film active absorption process, with its calcium, the bonded albumen form of phosphorus is served as carrier, make Ca, Fe, Zn, the turn-over capacity of P obviously strengthens, improve its absorption rate and food conversion utilization rate, wherein the absorbance of calcium can be up to 88%, the food conversion utilization rate can reach 32%, simultaneously other bivalent metal ion there is stronger absorption function, in addition, this material is to calcium phosphate and hydroxyapatite, has special affinity, can be in bony surface with calcium, phosphorus is converted into the bone mineral calmness, osteoporosis impels union of fracture, and this material also can be with calcium, phosphorus atoms conducts to tooth hydroxyapatite surface and permeates in tooth, make it be converted into the part of tooth, can also alleviate dental lamina acidity, bacteria growing inhibiting, and the inhibition demineralization is arranged, promote the function of remineralization, damaged tooth is reinforced, repair, in addition, also have of the effect of promotion fluoride in the dental lamina surface combination.This material energy enhancing human body immunity power, no any poison are paid effect, can add in the numerous food, make plurality kinds of health care food or directly do health medicine usefulness, and this material mainly adopts the immobilization proteinase preparation method, and this method cost is low, but continuous industry production.
Fig. 1 immobilization proteinase bioreactor and process chart.
Embodiment 1: with fresh skim milk 200g, place the 1000ml deionized water, adding lactic acid accent pH value is 2～5, centrifugal 8000rpm, remove lactic acid, supernatant is reduced pressure, recovery albumen is standby, precipitation is suspended in the 500ml deionized water, adding 0.1～0.5% glucose calcium salt adjusting pH value is 7.5, place it in bioreactor (2) of band hot water jacket layer from charging aperture (1), make tryptic carrier with chitin deacetylase chitin mixture (1: 1), adopt investment to be made into the graininess immobilized enzyme, pack with Nylon Bag, be contained on the stirrer (3) in the bioreactor (2), temperature in the bioreactor (2) is 55 ℃, reaction 0.5hr, with the centrifugal 1000rpm of reactant liquor, gained supernatant filter membrane is micropore filter (5) filter pressing of 0.45 μ, and unfiltered material returns through returning charge pipeline (4) and continue reaction in the bioreactor (2), filterable clear liquid is abandoned liquid outlet (7) through having, the dual ultrafilter (6) of molecular cut off 1000 enters freezer dryer (8), as Fig. 1, make finished product, its yield 25%, calcic 2.8g/100g, phosphorous 1.1g/100g, amino acid content is as follows:
Asparatate 5.2 threonines 3.3 serines 10.8 glutamic acid 15.27 isoleucines 3.16 leucines 4.5 tyrosine 1.85 glycine 1.30 phenylalanines 2.99 alanine 2.02 lysines 4.86 cystines trace arginase 12 .12 valine 4.08 proline 3 .53 methionine 0.99 histidine 0.84;
Wherein, as the protease carrier except that chitin deacetylase chitin mixture (1: 1, also can adopt cellular glass, resin anion (R.A.), ABSE-cellulose, enzyme preparation is removed trypsin also can select neutrality or alkaline protease for use to mend, as protease 1398, protease 2709, pancreatin, papain, process for fixation also can adopt investment or coupling method according to different carriers.
Embodiment 2: with fresh skim milk 200g, place the 1000ml deionized water, adding lactic acid accent pH value is 2～5, centrifugal 8000rpm, remove lactic acid, supernatant is reduced pressure, recovery albumen is standby, and precipitation is suspended in the 500ml deionized water, and adding 0.1～0.5% glucose calcium salt adjusting pH value is 7.5, place it in bioreactor (2) of band hot water jacket layer from charging aperture (1), make tryptic carrier with chitin deacetylase chitin mixture (1: 1), adopt investment to be made into the granulated immobilized enzyme, pack with Nylon Bag, be contained on the stirrer (3) in the bioreactor (2), 55 ℃ of temperature in the bioreactor (2), reaction 0.5hr adds 0.5 times 95% frozen alcohol in the reactant liquor, centrifugal 1000rpm, gained material drying device (8) vacuum drying, its yield 5%, calcic 7g/100g, phosphorous 2g/100g, aminoacid total content are 88g/100g.
Embodiment 3, with embodiment 1, but in course of reaction, can be with on-fixed protease preparation method in the bioreactor (2) of band hot water jacket layer.
Embodiment 4, are mineral nitrogen absorption factor peptide calcium preparation fruity peptide calcium beverage with the finished product that makes, its component: (g%)
Peptide calcium 3 skim milks 5 CMC0.3 fruit juice 90 flavoring agents, essence are an amount of
Embodiment 5, carbonated beverage, its component: (g%)
Peptide calcium 1.5 fruit juice, sugar, an amount of filling carbon dioxide of essence
Embodiment 6, its component of bone strengthening instant drink: (g%)
An amount of saccharic acid of peptide calcium 8 ferrous lactates, 0.2 Vc0.1 flavoring agent is adjusted to 100
Embodiment 7, peptide calcium capsule or tablet, its component: (g%)
Peptide calcium 1g magnesium stearate 0.5g starch 0.5g makes 2g sheet, grain.
Embodiment 8, chewing gum, its component: (g%)
Peptide calcium 3 mannitol, an amount of gum base 20 disproportionation Saccharum Sinensis Roxb. of sorbitol 40 flavoring agents are an amount of
Embodiment 9, fluoride dental cream, its component: (g%)
An amount of hibitane of peptide calcium 3 sodium fluoride, 0.5 SDS, Oleum menthae, microcrystalline Cellulose, starch flavoring agent are an amount of
Embodiment 10, its component of toothpaste: (g%)
Peptide calcium 2 glycerol 30 phosphides 0.5 SDS0.5 starch 30 deionized waters 30
Embodiment 11, milk slab sugar, its component: (g%)
Peptide calcium 2 milk powder 3 gelatin 0.1 disproportionation Saccharum Sinensis Roxb. 70
Embodiment 12, mouthful Su liquid, its component: (g%)
Peptide calcium 3 SDS0.5 hibitane 0.1 Oleum menthae suitable quantity of water 90
Embodiment 13, ice cream, its component: (g%)
Peptide calcium 8 starch 20 sugar 30 are aided with an amount of citric acid and emulsifying agent
Embodiment 14, cake, its component: (g%)
Peptide calcium 28 starch, egg are an amount of
Adnexa:
Test the test of 1. dental caries:
Round 4 pairs of the abnormal institute teeth of transferring from others, remove root of the tooth, ultrasonic waves for cleaning, emedullate, use 2molHCLO
4Soaked 1 minute, distilled water washes down, and smears with nial polish, reserves the district of windowing of 4mm * 4mm at labial surface.Be divided into lactic acid group, sugared salt group, each organizes experimental period is 8 days, and water temperature is 37 ℃, and tooth soaks every day 8 times, each 0.5hr.
The lactic acid group: (1) a pair of tooth is dipped in the lactic acid demineralization liquid, and demineralization liquid is formed: 0.1mol lactic acid, PH4.1, deionized water 100ml.
(2) a pair of tooth is immersed in and adds peptide calcium group, solution composition: 0.1mol lactic acid, 2% peptide calcium, deionized water 100ml.
Sugar salt group: (1) a pair of tooth is immersed in the sugared saline solution.The sugar saline solution is formed: 0.1g sodium chloride, 1g potassium chloride, 2g glucose, 2g sucrose, 0.1g calcium chloride, 100ml deionized water.
(2) a pair of tooth is immersed in and adds peptide calcium group, and solution composition: other composition is the same, only adds peptide calcium 2%.
Result of the test, the lactic acid group: (1) group tooth is windowed to distinguish and the Chalk shape occurred, depression, tarnish is touched softer.Easy cut, microscopic test have also proved the infringement of dental caries sample.(2) group is windowed and distinguished no significant change, and is glossy, harder, is difficult to cut, and microscopic test does not have the infringement of dental caries sample.
Sugar salt group: (1) group is windowed to distinguish and is blured, the Chalk shape, and slight depression, facing is softer, and easy cut, microscopic test have the infringement of dental caries sample.(2) group is windowed and distinguished no significant change, and is glossy, harder, and microscopic test does not have the infringement of dental caries sample.
Test 2, the remineralization test
Learnt from else's experience cleaning, remove two pairs of whole abnormal people's teeth of root of the tooth, dental pulp, be dipped in artificial dental caries liquid, handled five, take out and clean.Be dipped in the remineralization liquid, every day 4 times, each 1hr, coprocessing 10 days is in 37 ℃ of water-baths.
Remineralization liquid: 2% peptide calcium, 50% saliva, deionized water is made into 100ml, regulates PH7.0.
Result of the test, the carious lesions case hardness significantly increases, and light Tianjin occurs, and the top layer slightly thickens, and it is flat that part has the position of ditch to mend substantially, microscopic test, there is crystal growth on the surface.
Test 3, the calcium absorption utilization rate is measured
Get 12 of wean back rats, be divided into two groups, put into metabolic cage, single only raising, the drink deionized water, ad lib is weighed before and after the experiment, collects excrement, urine, uses network and titration measuring calcium content, and 4 is an experimental period.The activated calcium group is a matched group.Activated calcium is the commercial preparation, calcic 10.16%.(1) group feedstuff: A 45g+ peptide calcium 6.65g(every day); (2) group feedstuff: A 45g+ activated calcium powder 10g(every day).Wherein the A component is: beautiful material powder 90%, sugar 5%, sodium chloride 0.2%.Experimental result sees Table 1,2.
Table 1
The calcium metabolism result
Group is taken in Ca (mg) urine Ca (mg) excrement Ca (mg) absorbance (%) utilization rate (%)
(1) 382 5.1 44.8 88.2 86.9
6
(2) 375 40.1 110.3 70.5 59.8
6
Absorbance=(taking in Ca-excrement Ca)/(taking in Ca) utilization rate=(taking in Ca-(excrement Ca+ urine Ca))/(taking in Ca)
Table 2
Mus body weight increment measurement result
Body weight (g) experiment back body weight (g) weightening finish (g) efficiency of feed utilization (%) before the group experiment
(1) 445 510.2 65 31.46
6
(2) 450 454 4 1.8
6
Efficiency of feed utilization=(gain in weight)/(dietary intake) * 100%
Claims (7)
1, a kind of mineral nitrogen absorption factor peptide calcium, by natural phosphor protein through enzyme hydrolysis, be prepared into powdered substance, it is the mixture of polypeptide and calcium, phosphoric acid chelating, have phosphoserine calcium, phosphothreonine calcium structure in the peptide chain, this material is in the scope of PH2-12, and is soluble in water, meet carbonic acid and do not precipitate, its component is: (g/100g)
Calcium 1.5～7 phosphorus 0.5～2 total amino acid content 60～90 is wherein:
Asparatate 4～6.5 threonines 2～4 serines 3～18 proline 3s～5 glutamic acid, 15～22 alanine, 2～3 valines, 4～5 arginine, 1～3 cystine 0～micro-methionine 0.4～1 leucine 2～5 isoleucines 3～5 phenylalanines 1～3 lysine 1～5 histidine trace～1 glycine, 1～2 tyrosine trace～2.
2, mineral nitrogen absorption factor peptide calcium according to claim 1 is characterized in that a certain proportion of mineral nitrogen absorption factor peptide calcium is equipped with other adjuvant, and making various preparations becomes health food, also can directly use as the health care medicine.
(1) fruity peptide calcium beverage, its component: (g%)
Peptide calcium 2～4 skim milks 5 CMC0.3 flavoring agents, an amount of fruit juice 90 of essence
(2) carbonated beverage, its component: (g%)
Peptide calcium 0.5～2 fruit juice, sugar, an amount of filling carbon dioxide of essence
(3) bone strengthening electuary, its component: (g%)
Peptide calcium 5～10 ferrous lactates 0.2 vitamin C is equipped with saccharic acid in right amount and is adjusted to 100
(4) peptide calcium tablet or capsule, its component (g%)
Peptide calcium 1g/ sheet, grain are equipped with sheet, the grain that magnesium stearate 0.4-0.6 starch 0.4-0.8 makes 2-3g
(5) chewing gum, its component: (g%)
Peptide calcium 2～5 mannitol, an amount of gum base 20-30 of sorbitol aspartame 30-60 flavoring agent are an amount of
(6) toothpaste, its component: (g%)
Peptide calcium 1～5 glycerol 30 phosphides 0.5 SDS0.5 starch 30
(7) toothpaste with fluoride, its component: (g%)
An amount of microcrystalline Cellulose of peptide calcium 2～5 sodium fluoride, 0.5 SDS, an amount of flavoring agent of starch are an amount of
(8) mouthful Su liquid, its component: (g%)
Peptide calcium 1～5 SDS0.5 hibitane 0.1 spice suitable quantity of water 90
(9) milk sugar-tablet, its component: (g%)
An amount of aspartame 70-90 of peptide calcium 1～5 milk powder 3 gelatin
3, a kind of preparation method of mineral nitrogen absorption factor peptide calcium comprises the steps:
(1) with natural phosphoprotein, be suspended in the deionized water as yolk, frumentum, beans, newborn class, adopt isoelectric point precipitation to prepare pure phosphoprotein;
(2) pure phosphoprotein is placed the bioreactor that contains immobilization proteinase, add 2-10 times of deionized water, make suspension, regulate PH6～11 with glucose or fructose calcium salt, in 30～60 ℃ of temperature ranges, reaction 0.5～8hr;
(3) the micropore filter filter pressing that is 0.45 μ with reacted liquid filter membrane, filtrate is the dual ultrafiltration of 500～1000 filter membranes through molecular cut off, opens dryly with trapped substance is freezing, makes powdered substance-mineral nitrogen absorption factor peptide calcium.
4, the preparation method of mineral nitrogen absorption factor peptide calcium according to claim 3, it is characterized in that will be in the bioreactor through 0.5～8hr reaction, the reactant liquor of gained adds 0.5～10 times 95% frozen alcohol, centrifugal again vacuum drying makes finished product.
5, the mineral nitrogen factor according to claim 3 absorbs the preparation method of peptide calcium, the bioreactor (2) that it is characterized in that said band hot water jacket layer, include charging aperture (1), the stirrer (3) of immobilization proteinase is housed, this bioreactor communicates with microfilter (5) by the pipe connection, its microfilter (5) communicates with bioreactor (2) returning charge mouth by returning charge pipeline (4), and the dual ultrafilter (6) that microfilter (5) is abandoned liquid outlet (7) through pipeline and band is connected with freezer dryer (8).
6, the mineral nitrogen factor according to claim 5 absorbs the preparation method of peptide calcium, it is characterized in that said immobilization proteinase is to adopt protease 2709, also can adopt protease 1398, trypsin, pancreatin, papain, with cellular glass or resin anion (R.A.), ABSE-cellulose, chitin deacetylase chitin mixture is carrier, with coupling method or investment, be made into the graininess immobilization proteinase, pack, be installed on the stirrer (3) with Nylon Bag.
7, the preparation method of mineral nitrogen absorption factor peptide calcium according to claim 3 is characterized in that said reaction in the bioreactor (2) of band hot water jacket layer can adopt on-fixed enzyme preparation method.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1295979C (en) * | 2001-12-12 | 2007-01-24 | 高丽大学校 | Soybean phosphopeptide calcium and method for producing thereof |
| CN1305530C (en) * | 1996-03-05 | 2007-03-21 | 纽约州立大学研究基金会 | Anti-caries oral compositions |
| CN101015580B (en) * | 2006-12-12 | 2010-04-14 | 广东医学院 | pH-dependent compound bone peptide preparation and its preparation method and use |
| CN106420437A (en) * | 2016-07-07 | 2017-02-22 | 张义淦 | Plant mothproof bacteriostatic toothpaste and preparing method thereof |
| CN109363950A (en) * | 2018-11-27 | 2019-02-22 | 吉林省登泰克牙科材料有限公司 | A kind of gel combination, preparation method and application removing detritus for tooth root canal |
-
1993
- 1993-04-27 CN CN 93111085 patent/CN1094309A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305530C (en) * | 1996-03-05 | 2007-03-21 | 纽约州立大学研究基金会 | Anti-caries oral compositions |
| CN1295979C (en) * | 2001-12-12 | 2007-01-24 | 高丽大学校 | Soybean phosphopeptide calcium and method for producing thereof |
| CN101015580B (en) * | 2006-12-12 | 2010-04-14 | 广东医学院 | pH-dependent compound bone peptide preparation and its preparation method and use |
| CN106420437A (en) * | 2016-07-07 | 2017-02-22 | 张义淦 | Plant mothproof bacteriostatic toothpaste and preparing method thereof |
| CN109363950A (en) * | 2018-11-27 | 2019-02-22 | 吉林省登泰克牙科材料有限公司 | A kind of gel combination, preparation method and application removing detritus for tooth root canal |
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