CN106174165A - A kind of short flash extracting method of lycopene - Google Patents

Abstract

The invention discloses a quick flash extraction method of lycopene. The steps are as follows: firstly, fresh tomatoes are pretreated with absolute ethanol, and then ethyl acetate is used as a solvent to carry out the flash extraction process; The standard curve was used to calculate the average extraction rate. The invention has short extraction time and high extraction efficiency, and the extraction rate is 80% higher than that of the traditional solvent extraction method for 6 hours.

Description

A kind of rapid flash extraction method of lycopene

technical field

The invention relates to a method for extracting lycopene, in particular to a method for extracting lycopene in a flash.

Background technique

More than 80% of the human body's lycopene intake comes from tomatoes. Tomatoes, also known as tomatoes and persimmons, belong to the family Solanaceae. Native to America, Peru, Ecuador, Mexico and other countries, it is widely cultivated in the north and south of my country. Tomatoes are rich in vitamins, minerals, sugars, calcium, potassium, iron, zinc and other elements. Tomatoes can be used as both vegetables and fruits. Lycopene is a kind of carotenoid without oxygen. It is a functional natural pigment. It has strong anti-oxidation and the ability to scavenge free radicals. It also has anti-aging functions and can improve immunity. Preventing cardiovascular and cerebrovascular diseases and other effects, not only that, lycopene also has good effects on preventing and treating tumor patients, prostate cancer, uterine cancer, and middle-aged and elderly people with low immunity. It is a hot spot in the field of food research. Lycopene, a world-recognized functional food additive, has dual effects of nutritional coloring.

Lycopene is a fat-soluble pigment, insoluble in water, hardly soluble in strong polar solvents such as methanol, ethanol, etc., soluble in organic solvents such as benzene, chloroform, ether, petroleum ether, ethane, etc., soluble in aliphatic hydrocarbons, aromatic hydrocarbons and Chlorinated hydrocarbons, lycopene is a natural red pigment in plants, with a strong absorption peak at 485nm wavelength. When the traditional solvent extraction method uses fresh tomatoes to extract lycopene, fresh tomatoes contain a lot of water, and the solvent is not easy to penetrate into the cells, resulting in low extraction efficiency. The most common way is to dry the tomatoes, crush them into powder and then extract them. , Since lycopene is a heat-sensitive and photosensitive substance, the drying process will lead to the loss of lycopene content.

Contents of the invention

The purpose of the present invention is to provide a quick flash extraction method of lycopene, by establishing a process combining ethanol dehydration pretreatment and organic solvent flash extraction, control the tomato pretreatment method, extraction solvent, extraction time, and extraction machine gear , Solid-liquid ratio to increase the extraction rate of lycopene.

To achieve the above object, the present invention adopts the following technical solutions:

A kind of fast flash type extraction method of lycopene among the present invention, specifically comprises following specific process steps:

(1) Take samples of fresh tomatoes, wash and cut them into smoothie machines, add ethanol solution containing 0.05% BHT at a volume ratio of 1:2, stir for 20 minutes, centrifuge at 4800r/min for 10 minutes and filter, then add ethanol solution to the filter residue, and again Stir for 20min, centrifuge at 4800r/min for 10min and filter to obtain filtrate and filter residue;

(2) Put the tomato filter residue and solvent obtained in step (1) into the flash extractor at a volume ratio of 1:35-45, the solvent is ethyl acetate or chloroform, and adjust the working gear to medium speed (3 gear) to extract for 25-35s, and centrifuge to obtain filtrate and filter residue;

(3) measure the volume and the absorbance thereof of the centrifuged filtrate of step (2), measure and calculate the content and the extraction rate of lycopene in the filtrate;

(4) Get the tomato filter residue after centrifugation in step (2) and repeat the extraction process of step (2), obtain the tomato filtrate and filter residue extracted for the second time after centrifugation;

(5) Measure the volume and absorbance of the filtrate extracted for the second time in step (4), measure and calculate the content and extraction rate of lycopene in the filtrate.

Further, the ethanol solution in the step (1) is absolute ethanol solution.

Further, the volume ratio of the tomato filter residue to the solvent in the step (2) is 1:40, and the solvent is ethyl acetate.

Further, the calculation method of filtrate lycopene content and extraction rate in described step (3) and step (5) is:

A. Prepare lycopene standard curve with Sudan Ⅰ pigment: Weigh 0.0250g of Sudan Ⅰ pigment, transfer it into a 50mL volumetric flask with absolute ethanol, and set the volume to the mark, shake well to obtain lycopene stock solution; accurately draw the Put 10mL of the stock solution in a 50mL volumetric flask, adjust the volume to the mark with absolute ethanol, and shake well; respectively draw 0.5, 1.0, 1.5, 2.0, and 2.5mL into a 50mL volumetric flask, dilute with absolute ethanol to the mark, and shake well to obtain the concentrations of Lycopene standard solutions of 0.5, 1.0, 1.5, 2.0, 2.5 μg/mL. With absolute ethanol as a blank sample, the maximum absorption wavelength is determined by spectral scanning, and the absorbance of the standard solution is measured under the maximum absorption wavelength condition, and the lycopene standard curve is drawn with the concentration as the abscissa and the absorbance as the ordinate;

B. After diluting the centrifuged filtrate obtained in steps (2) and (4), obtain the concentration of lycopene according to the absorbance value measured by the diluted filtrate lycopene against the lycopene standard curve;

C. Calculate the lycopene extraction rate according to the following formula:

Among them, C—the concentration of lycopene converted from the absorbance after appropriate dilution of the filtrate, μg/mL;

V—the volume of filtrate obtained by suction filtration, mL;

m—the mass of the sample fresh tomato, g.

The beneficial effects of the present invention are that, in the step (1) of the present invention, the use of absolute ethanol to treat twice is beneficial to the removal of water and alcohol-soluble impurities without causing a large amount of loss of lycopene, which in turn is conducive to improving the concentration of lycopene. Extraction efficiency and purity; the extraction rate of lycopene in ethyl acetate and chloroform is significantly higher than that of other organic solvents, and the use of ethyl acetate has less toxicity and lower cost; solid-liquid ratio, extraction time and extraction gear Reasonable, will not cause loss of lycopene due to overheating. Therefore, the present invention has short extraction time and high extraction efficiency, and its extraction rate is 80% higher than that of the traditional solvent extraction method for 6 hours.

Description of drawings

Fig. 1 is a standard curve diagram of lycopene in a quick flash extraction method of lycopene according to the present invention.

detailed description

The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and through specific implementation methods.

A kind of fast flash type extraction method of lycopene among the present invention, specifically comprises following specific process steps:

(1) Take dozens of fresh tomatoes, wash and cut them into smoothie machines, add an absolute ethanol solution containing 0.05% BHT at a volume ratio of 1:2, stir for 20 minutes, centrifuge at 4800r/min for 10 minutes, and filter to obtain the filter residue. Add absolute ethanol solution, stir again for 20min, centrifuge at 4800r/min for 10min and filter to obtain filtrate and filter residue;

(2) Take 5 grams of the tomato filter residue obtained in step (1) and put it into a flash extractor at a volume ratio of 1:40 with a solvent, the solvent is ethyl acetate, adjust the working gear to the third gear and extract for 30 seconds, and centrifuge to obtain Tomato filtrate and residue;

(3) measure the volume and the absorbance thereof of the filtrate after step (2) centrifugation, measure and calculate the content and the extraction rate of lycopene in the filtrate:

(4) Get the tomato filter residue after centrifugation in step (2) and repeat the extraction process of step (2), obtain tomato filtrate and filter residue after secondary centrifugation;

(5) Measure the volume and absorbance of the filtrate after secondary centrifugation in step (4), measure and calculate the content and extraction rate of lycopene in the filtrate.

In this instance, the specific steps of the method for calculating the extraction rate in steps (3) and (5) are:

A. Prepare lycopene standard curve with Sudan Ⅰ pigment: Weigh 0.0250g of Sudan Ⅰ pigment, transfer it into a 50mL volumetric flask with absolute ethanol, and set the volume to the mark, shake well to obtain lycopene stock solution; accurately draw the Put 10mL of the stock solution in a 50mL volumetric flask, adjust the volume to the mark with absolute ethanol, and shake well; respectively draw 0.5, 1.0, 1.5, 2.0, and 2.5mL into a 50mL volumetric flask, dilute with absolute ethanol to the mark, and shake well to obtain the concentrations of Lycopene standard solutions of 0.5, 1.0, 1.5, 2.0, 2.5 μg/mL. With absolute ethanol as a blank sample, the maximum absorption wavelength is determined by spectral scanning, and the absorbance of the standard solution is measured under the maximum absorption wavelength condition, and the lycopene standard curve is drawn with the concentration as the abscissa and the absorbance as the ordinate;

B. After diluting the centrifuged filtrate obtained in steps (2) and (4), obtain the concentration of lycopene according to the absorbance value measured by the diluted filtrate lycopene against the lycopene standard curve;

C. Calculate the lycopene extraction rate according to the following formula:

Among them, C—the concentration of lycopene converted from the absorbance after appropriate dilution of the filtrate, μg/mL;

V—the volume of filtrate obtained by suction filtration, mL;

m—the mass of the sample fresh tomato, g.

Based on the above, the measured average extraction rate of lycopene is 88.037 μg/g, while the extraction rate of traditional solvent extraction method is generally 47 μg/g. It can be seen that the flash extraction method in the present invention has the characteristics of fast and high efficiency.

Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (4)

1. the short flash extracting method of a lycopene, it is characterised in that include following concrete technology step:

(1) taking sample fresh tomato to clean stripping and slicing and put into Ice-making machine, 1:2 adds the ethanol solution containing 0.05%BHT by volume, Stirring 20min, 4800r/min filter after being centrifuged 10min, obtain filtering residue and add ethanol solution again, are again stirring for 20min, 4800r/min filters to get filtrate and filtering residue after being centrifuged 10min；

(2) Fructus Lycopersici esculenti filtering residue step (1) obtained and solvent are during 1:35-45 puts into flash extracter by volume, and solvent is Ethyl acetate or chloroform, extract 25-35s under regulation operation range to middling speed, be centrifuged and obtain filtrate and filtering residue；

(3) determination step (2) is centrifuged volume and the absorbance thereof of rear filtrate, calculates content and the extraction of lycopene in filtrate Rate；

(4) take the Fructus Lycopersici esculenti filtering residue after being centrifuged in step (2) and repeat the extraction process of step (2), after secondary centrifuging, obtain Fructus Lycopersici esculenti filter Liquid and filtering residue；

(5) volume of filtrate after secondary centrifuging and absorbance thereof in determination step (4), calculates containing of lycopene in filtrate Amount and extraction ratio.

The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step (1) in, ethanol solution is ethanol solution.

The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step (2) in, Fructus Lycopersici esculenti filtering residue is 1:40 with the volume ratio of solvent, and described solvent is ethyl acetate.

The short flash extracting method of a kind of lycopene the most according to claim 1, it is characterised in that described step (3) measuring method of filtrate content of lycopene and extraction ratio is and in (5):

A. lycopene standard curve is made with S Ⅰ pigment: weigh 0.0250g S Ⅰ pigment, move into dehydrated alcohol 50mL volumetric flask, and it is settled to scale, shake up to obtain lycopene storing solution；Accurately draw this storing solution 10mL in 50mL capacity Bottle, dehydrated alcohol is settled to scale, shakes up；Draw 0.5 respectively, 1.0,1.5,2.0,2.5mL is in 50mL volumetric flask, dehydrated alcohol Be diluted to scale, shake up concentration is respectively the lycopene standard solution of 0.5,1.0,1.5,2.0,2.5 μ g/mL.With anhydrous Ethanol is blank sample, and spectral scan determines maximum absorption wavelength, and under the conditions of this maximum absorption wavelength, bioassay standard solution is inhaled Luminosity, with concentration as abscissa, absorbance be vertical coordinate draw lycopene standard curve；

B. after the filtrate dilution after being centrifuged in step (2) and (4), the suction measured according to filtrate lycopene after dilution Shading value comparison lycopene standard curve draws the concentration of lycopene；

C. according to equation below calculating lycopene:

Wherein, C suitably dilute according to filtrate after the absorbance concentration of lycopene that converts out, μ g/mL；

The filtrate volume that V sucking filtration obtains, mL；

The quality of m sample fresh tomato, g.