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CL2016001134A1 - Celula t o celula asesina natural (nk) que expresa dos receptores de antigeno quimerico (car), donde un car comprende un endodominio activador y el otro un dominio inhibitorio de proteina tirosina fosfatasa que contiene el dominio src (sh2); acido nucleico; vector; metodo de produccion; composicion; uso para preparar medicamento. - Google Patents

Celula t o celula asesina natural (nk) que expresa dos receptores de antigeno quimerico (car), donde un car comprende un endodominio activador y el otro un dominio inhibitorio de proteina tirosina fosfatasa que contiene el dominio src (sh2); acido nucleico; vector; metodo de produccion; composicion; uso para preparar medicamento.

Info

Publication number
CL2016001134A1
CL2016001134A1 CL2016001134A CL2016001134A CL2016001134A1 CL 2016001134 A1 CL2016001134 A1 CL 2016001134A1 CL 2016001134 A CL2016001134 A CL 2016001134A CL 2016001134 A CL2016001134 A CL 2016001134A CL 2016001134 A1 CL2016001134 A1 CL 2016001134A1
Authority
CL
Chile
Prior art keywords
car
domain
cell
endodomain
nucleic acid
Prior art date
Application number
CL2016001134A
Other languages
English (en)
Inventor
Martin Pule
Khai Kong
Shaun Cordoba
Original Assignee
Ucl Business Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201320573A external-priority patent/GB201320573D0/en
Priority claimed from GB201410934A external-priority patent/GB201410934D0/en
Application filed by Ucl Business Plc filed Critical Ucl Business Plc
Publication of CL2016001134A1 publication Critical patent/CL2016001134A1/es

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Abstract

Se describe una célula T que coexpresa un primer receptor antigénico (CAR) y un segundo CAR en la superficie celular, y cada CAR comprende: (i) un dominio de unión a antígenos, (ii) un espaciador, (iii) un dominio transmembranal; y (iv) un endodominio, donde los dominios de unión a antígeno del primer y segundo CAR se unen a diferentes antígenos, y en donde uno del primer o segundo CAR es un CAR activador que comprende un endodominio activador, y el otro CAR es un CAR inhibitorio que comprende un endodominio inhibitorio de ligación encendida que comprende un dominio de tirosina fosfatasa de una proteína tirosina fosfatasa que contiene el dominio homologo Src (SH2); una secuencia de ácido nucleico que codifica dichos primer y segundo CAR; un kit que comprende las secuencia de ácido nucleico que codifican dichos primer y segundo CAR; un kit que comprende un primer y un segundo vector que comprenden dichas secuencias de ácido nucleico; un método para elaborar dicha célula T; una composición farmacéutica que comprende dichas células T y un vehículo, diluyente o excipiente farmacéuticamente aceptable; el uso de diha composición farmacéutica para preparar un medicamento útil para tratar y/o prevenir el cáncer; el uso de dichas células T para preparar un medicamento para tratar y/o prevenir el cáncer; una célula asesina natural (NK) que coexpresa un primer receptor antigénico (CAR) y un segundo CAR en la superficie celular, y cada CAR comprende: (i) un dominio de unión a antígenos, (ii) un espaciador, (iii) un dominio transmembranal; y (iv) un endodominio, donde los dominios de unión a antígenos del primer y segundo CAR se unen a diferentes antígenos, y en donde uno del primer o segundo CAR es un CAR activador que comprende un endodominio activador, y el otro CAR es un CAR inhibitorio que comprende un endodominio inhibitorio de ligación encendida que comprende un dominio de tirosina fosfatasa de una proteína tirosina fosfatasa que contiene el dominio homologo Src (SH2); y una composición de células que comprende las células T que expresan CAR y/o células NK que expresan CAR de acuerdo con la reivindicación 20, elaborada al transducir una muestra de sangre ex vivo con un ácido nucleico que codifica el primer y segundo CAR.
CL2016001134A 2013-11-21 2016-05-12 Celula t o celula asesina natural (nk) que expresa dos receptores de antigeno quimerico (car), donde un car comprende un endodominio activador y el otro un dominio inhibitorio de proteina tirosina fosfatasa que contiene el dominio src (sh2); acido nucleico; vector; metodo de produccion; composicion; uso para preparar medicamento. CL2016001134A1 (es)

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GB201320573A GB201320573D0 (en) 2013-11-21 2013-11-21 T Cell
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CL2016001135A CL2016001135A1 (es) 2013-11-21 2016-05-12 Celula que coexpresa un primer receptor antigenico quimerico (car) y un segundo car en la superficie celular, y cada car comprende un dominio de unión a antígenos, un espaciador, un dominio transmembranal y un endodominio.
CL2016001134A CL2016001134A1 (es) 2013-11-21 2016-05-12 Celula t o celula asesina natural (nk) que expresa dos receptores de antigeno quimerico (car), donde un car comprende un endodominio activador y el otro un dominio inhibitorio de proteina tirosina fosfatasa que contiene el dominio src (sh2); acido nucleico; vector; metodo de produccion; composicion; uso para preparar medicamento.

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CL2016001135A CL2016001135A1 (es) 2013-11-21 2016-05-12 Celula que coexpresa un primer receptor antigenico quimerico (car) y un segundo car en la superficie celular, y cada car comprende un dominio de unión a antígenos, un espaciador, un dominio transmembranal y un endodominio.

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