CL2013001596A1 - Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). - Google Patents
Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3).Info
- Publication number
- CL2013001596A1 CL2013001596A1 CL2013001596A CL2013001596A CL2013001596A1 CL 2013001596 A1 CL2013001596 A1 CL 2013001596A1 CL 2013001596 A CL2013001596 A CL 2013001596A CL 2013001596 A CL2013001596 A CL 2013001596A CL 2013001596 A1 CL2013001596 A1 CL 2013001596A1
- Authority
- CL
- Chile
- Prior art keywords
- gadh
- enzyme
- gluconate dehydrogenase
- stabilization
- purification
- Prior art date
Links
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 title 3
- 101710088194 Dehydrogenase Proteins 0.000 title 3
- 102000004190 Enzymes Human genes 0.000 title 3
- 108090000790 Enzymes Proteins 0.000 title 3
- 229940050410 gluconate Drugs 0.000 title 3
- 238000000034 method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 230000006641 stabilisation Effects 0.000 title 1
- 238000011105 stabilization Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
- C12H1/14—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
- C12Y101/99003—Gluconate 2-dehydrogenase (acceptor) (1.1.99.3)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201031819A ES2387661B1 (es) | 2010-12-10 | 2010-12-10 | Proceso de purificación y estabilización de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2013001596A1 true CL2013001596A1 (es) | 2014-04-11 |
Family
ID=46206647
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2013001596A CL2013001596A1 (es) | 2010-12-10 | 2013-06-05 | Proceso de purificacion y estabilizacion de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20130260407A1 (es) |
| EP (1) | EP2650363A4 (es) |
| CL (1) | CL2013001596A1 (es) |
| ES (1) | ES2387661B1 (es) |
| WO (1) | WO2012076738A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148257B (zh) * | 2015-04-13 | 2019-08-13 | 中国科学院上海高等研究院 | 改造的克雷伯氏肺炎杆菌及其生产葡萄糖酸的应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3928209A1 (de) * | 1989-08-25 | 1991-02-28 | Boehringer Mannheim Gmbh | D-malat-bestimmung |
| DE3936757A1 (de) * | 1989-11-04 | 1991-05-08 | Forschungszentrum Juelich Gmbh | Verfahren zur gewinnung von sorbit und gluconsaeure bzw. gluconat und dafuer geeignete zellmassen |
| US5861295A (en) * | 1997-01-02 | 1999-01-19 | Life Technologies, Inc. | Nucleic acid-free thermostable enzymes and methods of production thereof |
| KR100257117B1 (ko) * | 1997-09-25 | 2000-05-15 | 박호군 | 세포막 결합 글루코네이트 탈수소 효소 및 그 유전자와 형질전환된 재조합 대장균을 이용한 2-케토-d-글루코네이트로의 생산 |
| WO2001042456A2 (en) * | 1999-12-10 | 2001-06-14 | Genespan Corporation | Isolation and purification of nucleic acids |
| EP1254944B1 (en) * | 1999-12-24 | 2007-03-28 | Otsuka Pharmaceutical Co., Ltd. | Dry compositions containing hydrophobic amino acid |
| DE60229050D1 (de) * | 2001-04-04 | 2008-11-06 | Genencor Int | Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen |
| KR101227226B1 (ko) * | 2004-07-07 | 2013-01-28 | 디에스엠 아이피 어셋츠 비.브이. | 개선된 효소 |
| US7928052B2 (en) * | 2004-12-09 | 2011-04-19 | Dow Global Technologies Llc | Enzyme stabilization |
| US20080003628A1 (en) * | 2006-03-31 | 2008-01-03 | Toyo Boseki Kabushiki Kaisha | Method for enhancing stability of a composition comprising soluble glucose dehydrogenase (gdh) |
| RU2470996C2 (ru) * | 2007-01-11 | 2012-12-27 | ДАНИСКО ЮЭс ИНК., ДЖЕНЕНКОР ДИВИЖН | Способ получения белка |
| ES2312282B1 (es) | 2007-08-02 | 2010-02-08 | Universidad De Cadiz | Metodo de atrapamiento e inmovilizacion de enzimas oxidoreductasas y sus usos. |
| ES2315197B1 (es) | 2007-09-14 | 2010-02-08 | Universidad De Cadiz. | Nuevo biosensor amperometrico, procedimiento de fabricacion y usos. |
| GB0812041D0 (en) * | 2008-07-02 | 2008-08-06 | Enigma Diagnostics Ltd | Compositions |
| EP2379733A2 (en) * | 2008-12-19 | 2011-10-26 | Novozymes Inc. | Methods for increasing hydrolysis of cellulosic material |
| CA2748232A1 (en) * | 2008-12-24 | 2010-07-22 | Comvita New Zealand Limited | Medical and nutritional formulations |
-
2010
- 2010-12-10 ES ES201031819A patent/ES2387661B1/es active Active
-
2011
- 2011-11-18 US US13/992,980 patent/US20130260407A1/en not_active Abandoned
- 2011-11-18 EP EP11846384.3A patent/EP2650363A4/en not_active Withdrawn
- 2011-11-18 WO PCT/ES2011/070794 patent/WO2012076738A1/es not_active Ceased
-
2013
- 2013-06-05 CL CL2013001596A patent/CL2013001596A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2387661A1 (es) | 2012-09-27 |
| EP2650363A4 (en) | 2014-10-15 |
| EP2650363A1 (en) | 2013-10-16 |
| US20130260407A1 (en) | 2013-10-03 |
| WO2012076738A1 (es) | 2012-06-14 |
| ES2387661B1 (es) | 2013-10-31 |
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