CA2978123A1 - Systeme de microscopie sted multicanal en temps reel - Google Patents
Systeme de microscopie sted multicanal en temps reel Download PDFInfo
- Publication number
- CA2978123A1 CA2978123A1 CA2978123A CA2978123A CA2978123A1 CA 2978123 A1 CA2978123 A1 CA 2978123A1 CA 2978123 A CA2978123 A CA 2978123A CA 2978123 A CA2978123 A CA 2978123A CA 2978123 A1 CA2978123 A1 CA 2978123A1
- Authority
- CA
- Canada
- Prior art keywords
- imaging
- light
- sted
- radiation
- beamlets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000010870 STED microscopy Methods 0.000 title claims abstract description 64
- 238000003384 imaging method Methods 0.000 claims abstract description 78
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- 238000009987 spinning Methods 0.000 claims description 26
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- 230000011218 segmentation Effects 0.000 claims description 21
- 150000001875 compounds Chemical class 0.000 claims description 15
- 238000000799 fluorescence microscopy Methods 0.000 claims description 15
- 238000000386 microscopy Methods 0.000 claims description 15
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/0044—Scanning details, e.g. scanning stages moving apertures, e.g. Nipkow disks, rotating lens arrays
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2978123A CA2978123A1 (fr) | 2017-09-05 | 2017-09-05 | Systeme de microscopie sted multicanal en temps reel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2978123A CA2978123A1 (fr) | 2017-09-05 | 2017-09-05 | Systeme de microscopie sted multicanal en temps reel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2978123A1 true CA2978123A1 (fr) | 2019-03-05 |
Family
ID=65632965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2978123A Abandoned CA2978123A1 (fr) | 2017-09-05 | 2017-09-05 | Systeme de microscopie sted multicanal en temps reel |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2978123A1 (fr) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111122526A (zh) * | 2019-12-18 | 2020-05-08 | 中国科学院南海海洋研究所 | 一种基于激光诱导荧光及流体动态显微摄影的水体藻类检测方法 |
| CN111474150A (zh) * | 2020-04-08 | 2020-07-31 | 华南师范大学 | 一种sted超分辨图像背景噪声差分抑制方法 |
| CN111653378A (zh) * | 2020-06-05 | 2020-09-11 | 桂林电子科技大学 | 基于多光纤光镊的sted超分辨显微成像装置 |
| EP3888838A1 (fr) * | 2020-03-12 | 2021-10-06 | Rohr, Inc. | Système et procédé de perforation de substrat utilisant des mini-faisceaux et un scanner |
| CN114280766A (zh) * | 2021-12-24 | 2022-04-05 | 哈尔滨工业大学 | 基于梯度折射率透镜的七目微型三维显微成像装置和方法 |
| CN116075763A (zh) * | 2020-08-07 | 2023-05-05 | 阿贝锐纳仪器有限公司 | 估计样品中发射体位置的方法、器件、显微镜和计算机程序 |
| CN116068745A (zh) * | 2023-02-24 | 2023-05-05 | 广东粤港澳大湾区协同创新研究院 | 一种角度连续可调超分辨显微镜照明装置及相应成像方法 |
| CN116235093A (zh) * | 2020-08-07 | 2023-06-06 | 阿贝锐纳仪器有限公司 | 用于定位样本中的发射体的方法、设备和计算机程序 |
| CN116337829A (zh) * | 2023-02-16 | 2023-06-27 | 之江实验室 | 一种高通道荧光辐射差分显微成像方法和装置 |
| EP4258036A1 (fr) * | 2022-04-07 | 2023-10-11 | Till I.D. GmbH | Dispositif de microscope à disque tournant avec une résolution d'image potentiellement améliorée |
| DE102023128709B3 (de) * | 2023-10-19 | 2025-03-20 | Abberior Instruments Gmbh | Verfahren, lichtmikroskop und computerprogramm zur lokalisierung oder zum verfolgen von emittern in einer probe |
| CN120760860A (zh) * | 2025-08-25 | 2025-10-10 | 武汉慧观生物科技有限公司 | 一种高光谱显微成像系统 |
-
2017
- 2017-09-05 CA CA2978123A patent/CA2978123A1/fr not_active Abandoned
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111122526A (zh) * | 2019-12-18 | 2020-05-08 | 中国科学院南海海洋研究所 | 一种基于激光诱导荧光及流体动态显微摄影的水体藻类检测方法 |
| EP3888838A1 (fr) * | 2020-03-12 | 2021-10-06 | Rohr, Inc. | Système et procédé de perforation de substrat utilisant des mini-faisceaux et un scanner |
| US12064830B2 (en) | 2020-03-12 | 2024-08-20 | Rohr, Inc. | Substrate perforation system and method using beamlets |
| CN111474150A (zh) * | 2020-04-08 | 2020-07-31 | 华南师范大学 | 一种sted超分辨图像背景噪声差分抑制方法 |
| CN111474150B (zh) * | 2020-04-08 | 2022-03-22 | 华南师范大学 | 一种sted超分辨图像背景噪声差分抑制方法 |
| CN111653378A (zh) * | 2020-06-05 | 2020-09-11 | 桂林电子科技大学 | 基于多光纤光镊的sted超分辨显微成像装置 |
| CN116075763A (zh) * | 2020-08-07 | 2023-05-05 | 阿贝锐纳仪器有限公司 | 估计样品中发射体位置的方法、器件、显微镜和计算机程序 |
| CN116235093A (zh) * | 2020-08-07 | 2023-06-06 | 阿贝锐纳仪器有限公司 | 用于定位样本中的发射体的方法、设备和计算机程序 |
| CN114280766A (zh) * | 2021-12-24 | 2022-04-05 | 哈尔滨工业大学 | 基于梯度折射率透镜的七目微型三维显微成像装置和方法 |
| EP4258036A1 (fr) * | 2022-04-07 | 2023-10-11 | Till I.D. GmbH | Dispositif de microscope à disque tournant avec une résolution d'image potentiellement améliorée |
| CN116337829A (zh) * | 2023-02-16 | 2023-06-27 | 之江实验室 | 一种高通道荧光辐射差分显微成像方法和装置 |
| CN116337829B (zh) * | 2023-02-16 | 2025-08-29 | 之江实验室 | 一种高通道荧光辐射差分显微成像方法和装置 |
| CN116068745A (zh) * | 2023-02-24 | 2023-05-05 | 广东粤港澳大湾区协同创新研究院 | 一种角度连续可调超分辨显微镜照明装置及相应成像方法 |
| DE102023128709B3 (de) * | 2023-10-19 | 2025-03-20 | Abberior Instruments Gmbh | Verfahren, lichtmikroskop und computerprogramm zur lokalisierung oder zum verfolgen von emittern in einer probe |
| CN120760860A (zh) * | 2025-08-25 | 2025-10-10 | 武汉慧观生物科技有限公司 | 一种高光谱显微成像系统 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |
Effective date: 20200908 |