CA2394827A1 - Recombinaison avec une sequence specifique d'adn catalysee par resolvase dans des cellules eucaryotes - Google Patents
Recombinaison avec une sequence specifique d'adn catalysee par resolvase dans des cellules eucaryotes Download PDFInfo
- Publication number
- CA2394827A1 CA2394827A1 CA002394827A CA2394827A CA2394827A1 CA 2394827 A1 CA2394827 A1 CA 2394827A1 CA 002394827 A CA002394827 A CA 002394827A CA 2394827 A CA2394827 A CA 2394827A CA 2394827 A1 CA2394827 A1 CA 2394827A1
- Authority
- CA
- Canada
- Prior art keywords
- resolvase
- sequence
- dna
- cell
- recombination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- 239000008207 working material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
L'invention concerne un procédé de recombinaison avec une séquence spécifique d'ADN dans des cellules eucaryotes comprenant l'introduction dans une cellule d'une première séquence d'ADN comprenant elle-même une séquence res, l'introduction d'une copie de la première séquence d'ADN et la réalisation de la recombinaison avec une séquence spécifique au moyen d'une résolvase ou d'un dérivé de celle-ci. L'invention concerne également un procédé pour tester des inhibiteurs de topoisomérase dans une cellule eucaryote, comprenant l'introduction dans une cellule d'une première séquence d'ADN conforme à SEQ ID NO:1, l'introduction dans une cellule d'une copie de la première séquence d'ADN conforme à SEQ ID NO:1 en orientation directe, l'introduction d'une résolvase de type sauvage et l'adjonction des inhibiteurs de topoisomérase à tester.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1999156513 DE19956513A1 (de) | 1999-11-24 | 1999-11-24 | Resolvase-katalysierte, Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
| DE19956513.9 | 1999-11-24 | ||
| PCT/EP2000/011718 WO2001038511A2 (fr) | 1999-11-24 | 2000-11-24 | Recombinaison avec une sequence specifique d'adn catalysee par resolvase dans des cellules eucaryotes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2394827A1 true CA2394827A1 (fr) | 2001-05-31 |
Family
ID=7930159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002394827A Abandoned CA2394827A1 (fr) | 1999-11-24 | 2000-11-24 | Recombinaison avec une sequence specifique d'adn catalysee par resolvase dans des cellules eucaryotes |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1240316A2 (fr) |
| JP (1) | JP2003533176A (fr) |
| AU (1) | AU1823901A (fr) |
| CA (1) | CA2394827A1 (fr) |
| DE (1) | DE19956513A1 (fr) |
| WO (1) | WO2001038511A2 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2739738B1 (fr) | 2011-08-03 | 2018-06-20 | Ramot at Tel Aviv University, Ltd. | Utilisation d'une intégrase pour l'expression génique ciblée |
-
1999
- 1999-11-24 DE DE1999156513 patent/DE19956513A1/de not_active Ceased
-
2000
- 2000-11-24 AU AU18239/01A patent/AU1823901A/en not_active Abandoned
- 2000-11-24 EP EP00977566A patent/EP1240316A2/fr not_active Withdrawn
- 2000-11-24 WO PCT/EP2000/011718 patent/WO2001038511A2/fr not_active Ceased
- 2000-11-24 JP JP2001540261A patent/JP2003533176A/ja active Pending
- 2000-11-24 CA CA002394827A patent/CA2394827A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE19956513A1 (de) | 2001-06-21 |
| AU1823901A (en) | 2001-06-04 |
| JP2003533176A (ja) | 2003-11-11 |
| WO2001038511A8 (fr) | 2002-05-30 |
| WO2001038511A2 (fr) | 2001-05-31 |
| EP1240316A2 (fr) | 2002-09-18 |
| WO2001038511A3 (fr) | 2002-05-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |