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BRPI1010035A2 - Células deficientes em fucosilação - Google Patents

Células deficientes em fucosilação

Info

Publication number
BRPI1010035A2
BRPI1010035A2 BRPI1010035-0A BRPI1010035A BRPI1010035A2 BR PI1010035 A2 BRPI1010035 A2 BR PI1010035A2 BR PI1010035 A BRPI1010035 A BR PI1010035A BR PI1010035 A2 BRPI1010035 A2 BR PI1010035A2
Authority
BR
Brazil
Prior art keywords
fucosylation
deficient cells
deficient
cells
Prior art date
Application number
BRPI1010035-0A
Other languages
English (en)
Inventor
Gang Chen
Darya Burakov
James P Fandl
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BRPI1010035A2 publication Critical patent/BRPI1010035A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271GDP-L-fucose synthase (1.1.1.271)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
BRPI1010035-0A 2009-06-02 2010-06-01 Células deficientes em fucosilação BRPI1010035A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18340009P 2009-06-02 2009-06-02
US34885810P 2010-05-27 2010-05-27
PCT/US2010/036914 WO2010141478A1 (en) 2009-06-02 2010-06-01 Fucosylation-deficient cells

Publications (1)

Publication Number Publication Date
BRPI1010035A2 true BRPI1010035A2 (pt) 2015-08-25

Family

ID=42338231

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1010035-0A BRPI1010035A2 (pt) 2009-06-02 2010-06-01 Células deficientes em fucosilação

Country Status (16)

Country Link
US (8) US8409838B2 (pt)
EP (3) EP3279326B1 (pt)
JP (4) JP5744856B2 (pt)
KR (2) KR101641526B1 (pt)
CN (1) CN102459603B (pt)
AU (1) AU2010256753B2 (pt)
BR (1) BRPI1010035A2 (pt)
CA (1) CA2764370C (pt)
DK (3) DK3279326T3 (pt)
ES (2) ES2661074T3 (pt)
HU (1) HUE038596T2 (pt)
IL (1) IL216624A (pt)
PL (3) PL2808393T3 (pt)
SG (1) SG176251A1 (pt)
TW (1) TWI513818B (pt)
WO (1) WO2010141478A1 (pt)

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TWI513818B (zh) 2009-06-02 2015-12-21 Regeneron Pharma 岩藻糖基化(fucosylation)-缺乏之細胞
CN109797138A (zh) 2011-03-06 2019-05-24 默克雪兰诺有限公司 低岩藻糖细胞系及其应用
WO2013013013A2 (en) * 2011-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for producing modified glycoproteins
TW201823460A (zh) 2012-05-29 2018-07-01 美商再生元醫藥公司 生產細胞株增強子
EP2722673B1 (en) 2012-10-17 2017-07-12 Hexal AG Improved method of mapping glycans of glycoproteins
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
US20160251410A1 (en) 2013-09-03 2016-09-01 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions and methods for expressing recombinant polypeptides
US20150093782A1 (en) * 2013-10-01 2015-04-02 The University Of Wyoming Compositions and methods for reducing fucosylation of glycoproteins in insect cells and methods of use thereof for production of recombinant glycoproteins
MY178160A (en) 2014-03-11 2020-10-06 Regeneron Pharma Anti-egfrviii antibodies and uses thereof
KR20250012733A (ko) * 2014-05-30 2025-01-24 뉴 잉글랜드 바이오랩스, 인크 탈당화 시약 및 방법
JP2017525340A (ja) * 2014-07-07 2017-09-07 ターガザイム,アイエヌシー. 治療的用途のためのフコシル化細胞の製造および凍結保存
CN105671109B (zh) * 2014-11-20 2019-08-23 中国人民解放军军事医学科学院生物工程研究所 用糖基工程酵母制备具有动物细胞糖基化修饰流感血凝素糖蛋白的方法
TWI710573B (zh) 2015-01-26 2020-11-21 美商再生元醫藥公司 抗伊波拉病毒醣蛋白之人類抗體
TWI797060B (zh) 2015-08-04 2023-04-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
MX2018004831A (es) * 2015-11-02 2018-08-01 Genentech Inc Metodos para confeccionar formas fucosiladas y afucosiladas de una proteina.
MX2018008447A (es) * 2016-01-06 2019-05-30 Oncobiologics Inc Modulacion de especies afucosilados en una composicion de anticuerpo monoclonal.
US11285210B2 (en) 2016-02-03 2022-03-29 Outlook Therapeutics, Inc. Buffer formulations for enhanced antibody stability
SG10202010155YA (en) * 2016-04-20 2020-11-27 Regeneron Pharma Compositions and methods for making antibodies based on use of expression-enhancing loci
CA3015371A1 (en) 2016-04-20 2017-10-26 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing locus
EP3339444A1 (en) * 2016-12-20 2018-06-27 Mabxience Research, S.L. Method for obtaining a glycoprotein with an increased percentage of afucosylated glycans
US12325737B2 (en) 2018-03-26 2025-06-10 Amgen Inc. Total afucosylated glycoforms of antibodies produced in cell culture
US20210317499A1 (en) * 2018-08-29 2021-10-14 United Biopharma Inc. Afucosylated antibodies and manufacture thereof
US20220002389A1 (en) 2018-10-29 2022-01-06 Immuno-Biological Laboratories Co., Ltd. Anti-hiv antibody and method for producing same
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
CA3179819A1 (en) 2020-05-26 2021-12-02 Alina Baum Anti-sars-cov-2-spike glycoprotein antibodies and antigen-binding fragments
KR20230019166A (ko) 2020-06-03 2023-02-07 리제너론 파아마슈티컬스, 인크. 항-SARS-CoV-2 스파이크 당단백질 항체를 이용한 SARS-CoV-2 감염 및 COVID-19 치료 또는 예방 방법
CN112843234B (zh) * 2021-01-20 2022-09-06 四川大学华西第二医院 一种调控生物机体内gdp-甘露糖的浓度的装置
EP4352094A1 (en) 2021-06-07 2024-04-17 Amgen Inc. Using fucosidase to control afucosylation level of glycosylated proteins
EP4359440A1 (en) 2021-06-22 2024-05-01 Regeneron Pharmaceuticals, Inc. Anti-egfrviii antibody drug conjugates and uses thereof
BR112024000744A2 (pt) 2021-07-14 2024-04-30 Regeneron Pharma Anticorpos de glicoprotéina spike anti-sars-cov-2 e fragmentos de ligação a antígeno
WO2023081434A2 (en) 2021-11-07 2023-05-11 Regeneron Pharmaceuticals, Inc. Methods for treating or preventing sars-cov-2 infections and covid-19 with anti-sars-cov-2 spike glycoprotein antibodies
KR20240167715A (ko) 2022-03-02 2024-11-27 리제너론 파아마슈티컬스, 인크. 고역가 항체의 제조 방법

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WO2010018847A1 (ja) 2008-08-13 2010-02-18 協和発酵キリン株式会社 遺伝子組換えプロテインs組成物
TWI513818B (zh) 2009-06-02 2015-12-21 Regeneron Pharma 岩藻糖基化(fucosylation)-缺乏之細胞
CN109797138A (zh) 2011-03-06 2019-05-24 默克雪兰诺有限公司 低岩藻糖细胞系及其应用

Also Published As

Publication number Publication date
HK1249547A1 (en) 2018-11-02
AU2010256753B2 (en) 2013-11-28
PL2438171T3 (pl) 2015-04-30
US20100304436A1 (en) 2010-12-02
AU2010256753A1 (en) 2012-01-12
HUE038596T2 (hu) 2018-10-29
HK1163745A1 (en) 2012-09-14
EP2438171B1 (en) 2014-12-17
EP3279326B1 (en) 2020-10-14
US20190024129A1 (en) 2019-01-24
KR20120029443A (ko) 2012-03-26
US20170211115A1 (en) 2017-07-27
US8409838B2 (en) 2013-04-02
JP5744856B2 (ja) 2015-07-08
EP2808393A2 (en) 2014-12-03
TWI513818B (zh) 2015-12-21
JP6211642B2 (ja) 2017-10-11
CN102459603B (zh) 2013-11-06
IL216624A0 (en) 2012-02-29
US20200080124A1 (en) 2020-03-12
EP2438171A1 (en) 2012-04-11
US20210254022A1 (en) 2021-08-19
US20160090410A1 (en) 2016-03-31
US10006070B2 (en) 2018-06-26
KR20140039340A (ko) 2014-04-01
JP2016104045A (ja) 2016-06-09
HK1198180A1 (en) 2015-03-13
DK2438171T3 (en) 2015-01-26
TW201107469A (en) 2011-03-01
US11560550B2 (en) 2023-01-24
US9206455B2 (en) 2015-12-08
US20130164786A1 (en) 2013-06-27
CA2764370A1 (en) 2010-12-09
PL3279326T3 (pl) 2021-03-08
US12054751B2 (en) 2024-08-06
JP2012528594A (ja) 2012-11-15
IL216624A (en) 2015-02-26
SG176251A1 (en) 2011-12-29
US20230295583A1 (en) 2023-09-21
KR101641526B1 (ko) 2016-07-21
KR101441437B1 (ko) 2014-09-25
EP2808393A3 (en) 2015-03-11
US9550823B2 (en) 2017-01-24
CA2764370C (en) 2018-08-21
DK2808393T3 (en) 2018-03-12
CN102459603A (zh) 2012-05-16
PL2808393T3 (pl) 2018-04-30
ES2661074T3 (es) 2018-03-27
WO2010141478A1 (en) 2010-12-09
JP2015051021A (ja) 2015-03-19
EP2808393B1 (en) 2018-01-10
ES2527173T3 (es) 2015-01-21
JP2017006147A (ja) 2017-01-12
DK3279326T3 (da) 2020-12-21
EP3279326A1 (en) 2018-02-07

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time