MX2018008447A - Modulacion de especies afucosilados en una composicion de anticuerpo monoclonal. - Google Patents
Modulacion de especies afucosilados en una composicion de anticuerpo monoclonal.Info
- Publication number
- MX2018008447A MX2018008447A MX2018008447A MX2018008447A MX2018008447A MX 2018008447 A MX2018008447 A MX 2018008447A MX 2018008447 A MX2018008447 A MX 2018008447A MX 2018008447 A MX2018008447 A MX 2018008447A MX 2018008447 A MX2018008447 A MX 2018008447A
- Authority
- MX
- Mexico
- Prior art keywords
- species
- level
- monoclonal antibodies
- culture
- modulation
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 abstract 2
- 238000001802 infusion Methods 0.000 abstract 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 abstract 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 abstract 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 230000004075 alteration Effects 0.000 abstract 1
- 239000012526 feed medium Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Las alteraciones en la alimentación de cultivo celular en biorreactor a una alimentación prolongada o continua después de un periodo inicial de no alimentación reduce el nivel de alto peso molecular, carga ácida, y las especies de fragmentos de anticuerpos monoclonales expresados en el cultivo, y mejora el nivel de las especies afucosiladas de los anticuerpos monoclonales expresados en el cultivo. Las infusiones de fucosa regulares después de un periodo inicial de no infusión de medio de alimentación reducen el nivel de especies afucosiladas de anticuerpos monoclonales expresados en el cultivo. La manipulación del cultivo celular puede utilizarse para modular el nivel de las especies de anticuerpos monoclonales.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662275384P | 2016-01-06 | 2016-01-06 | |
| PCT/US2017/012349 WO2017120347A1 (en) | 2016-01-06 | 2017-01-05 | Modulation of afucosylated species in a monoclonal antibody composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018008447A true MX2018008447A (es) | 2019-05-30 |
Family
ID=57944513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018008447A MX2018008447A (es) | 2016-01-06 | 2017-01-05 | Modulacion de especies afucosilados en una composicion de anticuerpo monoclonal. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20190161543A1 (es) |
| EP (1) | EP3400241B1 (es) |
| JP (1) | JP6674028B2 (es) |
| CN (1) | CN109153716A (es) |
| AU (1) | AU2017206006B2 (es) |
| CA (1) | CA3010598A1 (es) |
| MX (1) | MX2018008447A (es) |
| WO (1) | WO2017120347A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014337263B2 (en) | 2013-10-16 | 2019-12-12 | Outlook Therapeutics, Inc. | Buffer formulations for enhanced antibody stability |
| US10696735B2 (en) | 2015-01-21 | 2020-06-30 | Outlook Therapeutics, Inc. | Modulation of charge variants in a monoclonal antibody composition |
| CN109563161A (zh) | 2016-02-03 | 2019-04-02 | 安口生物公司 | 用于提高抗体稳定性的缓冲制剂 |
| CA3093853A1 (en) | 2018-03-26 | 2019-10-03 | Amgen Inc. | Total afucosylated glycoforms of antibodies produced in cell culture |
| HU231514B1 (hu) * | 2018-11-07 | 2024-07-28 | Richter Gedeon Nyrt. | Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer |
| CA3123591A1 (en) * | 2018-12-19 | 2020-06-25 | Seagen Inc. | Controlled fucosylation of antibodies |
| WO2021066772A1 (en) * | 2019-10-01 | 2021-04-08 | Arven Ilac Sanayi Ve Ticaret Anonim Sirketi | Cell culture medium for reducing fucosylation and basic variants in the production of antibodies |
| EP4291675A1 (en) * | 2021-02-12 | 2023-12-20 | Lonza Bend Inc. | Method for modulating afucoslyation of an antibody product |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6090382A (en) | 1996-02-09 | 2000-07-18 | Basf Aktiengesellschaft | Human antibodies that bind human TNFα |
| US8227241B2 (en) | 2004-03-12 | 2012-07-24 | Unigene Laboratories, Inc. | Bacterial host cell for the direct expression of peptides |
| CN101213294B (zh) | 2005-05-26 | 2013-03-27 | 赛托斯生物技术公司 | 可放大的发酵方法 |
| US7846724B2 (en) | 2006-04-11 | 2010-12-07 | Hoffmann-La Roche Inc. | Method for selecting CHO cell for production of glycosylated antibodies |
| JP5624535B2 (ja) * | 2008-05-02 | 2014-11-12 | シアトル ジェネティクス，インコーポレーテッド | 低いコアフコシル化を有する抗体及び抗体誘導体を調製するための方法並びに組成物 |
| KR101641526B1 (ko) | 2009-06-02 | 2016-07-21 | 리제너론 파마슈티칼스 인코포레이티드 | 푸코실화-결핍 세포 |
| AU2010256455A1 (en) * | 2009-06-05 | 2012-01-19 | Momenta Pharmaceuticals, Inc. | Methods of modulating fucosylation of glycoproteins |
| US20120258496A1 (en) | 2010-09-27 | 2012-10-11 | Boehringer Ingelheim International Gmbh | Production of low fucose antibodies in h4-ii-e rat cells |
| WO2013114165A1 (en) * | 2012-01-30 | 2013-08-08 | Dr Reddy's Laboratories Limited | Process of obtaining glycoprotein composition with increased afucosylation content |
| NO2760138T3 (es) * | 2012-10-01 | 2018-08-04 | ||
| WO2015140700A1 (en) * | 2014-03-19 | 2015-09-24 | Dr. Reddy's Laboratories Limited | Cell culture process |
| EP3371206B1 (en) * | 2015-11-02 | 2021-04-14 | F. Hoffmann-La Roche AG | Methods of making fucosylated and afucosylated forms of a protein |
-
2017
- 2017-01-05 MX MX2018008447A patent/MX2018008447A/es unknown
- 2017-01-05 CA CA3010598A patent/CA3010598A1/en active Pending
- 2017-01-05 US US16/067,193 patent/US20190161543A1/en not_active Abandoned
- 2017-01-05 EP EP17702444.5A patent/EP3400241B1/en not_active Revoked
- 2017-01-05 CN CN201780015362.XA patent/CN109153716A/zh active Pending
- 2017-01-05 JP JP2018535126A patent/JP6674028B2/ja active Active
- 2017-01-05 AU AU2017206006A patent/AU2017206006B2/en active Active
- 2017-01-05 WO PCT/US2017/012349 patent/WO2017120347A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP3400241A1 (en) | 2018-11-14 |
| US20190161543A1 (en) | 2019-05-30 |
| JP2019500876A (ja) | 2019-01-17 |
| CN109153716A (zh) | 2019-01-04 |
| JP6674028B2 (ja) | 2020-04-01 |
| CA3010598A1 (en) | 2017-07-13 |
| AU2017206006A1 (en) | 2018-07-19 |
| EP3400241B1 (en) | 2020-07-22 |
| AU2017206006B2 (en) | 2019-08-29 |
| WO2017120347A1 (en) | 2017-07-13 |
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