[go: up one dir, main page]

BRPI0918875A2 - Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura - Google Patents

Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura

Info

Publication number
BRPI0918875A2
BRPI0918875A2 BRPI0918875A BRPI0918875A BRPI0918875A2 BR PI0918875 A2 BRPI0918875 A2 BR PI0918875A2 BR PI0918875 A BRPI0918875 A BR PI0918875A BR PI0918875 A BRPI0918875 A BR PI0918875A BR PI0918875 A2 BRPI0918875 A2 BR PI0918875A2
Authority
BR
Brazil
Prior art keywords
microorganism
genetically modified
making
culture system
conversion
Prior art date
Application number
BRPI0918875A
Other languages
English (en)
Inventor
d lynch Michael
P Mercogliano Christopher
Original Assignee
Opx Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Opx Biotechnologies Inc filed Critical Opx Biotechnologies Inc
Publication of BRPI0918875A2 publication Critical patent/BRPI0918875A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MÉTODO PARA FAZER UM MICROORGANISMO GENETICAMENTE MODIFICADO, MICROORGANISMO E SISTEMA DE CULTURA. A presente invenção se refere a métodos, sistemas e composições, incluindo microorganismos geneticamente modificados, direcionados a obter redução de conversão microbiana de ácido 3-hidroxipropiônico (3-HP) para aldeídos de 3-HP. Em várias concretizações isto é obtido é obtido pela interrupção de genes particulares de aldeído desidrogenases, incluindo múltiplas deleções de genes. Entre os ácidos nucléicos específicos deletados em que a desejada redução de conversão é obtida estão aldA, aldB, puuC e usg do microorganismo E. Coli geneticamente modificado.
BRPI0918875A 2008-09-15 2009-09-15 Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura BRPI0918875A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9693708P 2008-09-15 2008-09-15
PCT/US2009/057058 WO2010031083A2 (en) 2008-09-15 2009-09-15 Methods, systems and compositions related to reduction of conversions of microbially produced 3-hydroxypropionic acid (3-hp) to aldehyde metabolites

Publications (1)

Publication Number Publication Date
BRPI0918875A2 true BRPI0918875A2 (pt) 2017-08-08

Family

ID=42005832

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0918875A BRPI0918875A2 (pt) 2008-09-15 2009-09-15 Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura

Country Status (4)

Country Link
US (2) US20130189787A1 (pt)
EP (1) EP2326709A4 (pt)
BR (1) BRPI0918875A2 (pt)
WO (1) WO2010031083A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2480673B1 (en) 2009-09-27 2018-05-23 OPX Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
US8809027B1 (en) * 2009-09-27 2014-08-19 Opx Biotechnologies, Inc. Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase
EP2529023A4 (en) * 2010-01-27 2015-06-10 Opx Biotechnologies Inc MICRO-ORGANISM MANUFACTURE FROM HIGH-FLOW CHEMICAL PRODUCTS AND CORRESPONDING COMPOSITIONS, METHODS AND SYSTEMS
KR20130119945A (ko) 2010-11-22 2013-11-01 노보자임스 인코포레이티드 3-히드록시프로피온산 생산을 위한 조성물 및 방법
US8728788B1 (en) 2011-09-30 2014-05-20 Novozymes A/S Dehydrogenase variants and polynucleotides encoding same
JP2015524283A (ja) 2012-08-10 2015-08-24 オーピーエックス バイオテクノロジーズ， インコーポレイテッド 脂肪酸および脂肪酸由来生成物の生成のため微生物および方法
US9868966B2 (en) 2012-10-11 2018-01-16 Technical University Of Denmark Genetically engineered yeast
CA2891130A1 (en) 2012-11-30 2014-06-05 Novozymes, Inc. 3-hydroxypropionic acid production by recombinant yeasts
US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
WO2014145096A1 (en) 2013-03-15 2014-09-18 Cindy Hoppe Flash evaporation for production purification and recovery
JP2016512688A (ja) 2013-03-15 2016-05-09 カーギル・インコーポレイテッド ３−ヒドロキシプロピオン酸の回収
US10337038B2 (en) 2013-07-19 2019-07-02 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN105431520A (zh) 2013-07-31 2016-03-23 诺维信公司 利用表达昆虫天冬氨酸1-脱羧酶的重组酵母的3-羟基丙酸生产
GB2528177B (en) 2014-06-11 2019-08-28 Univ Duke Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
US20180265902A1 (en) 2015-08-24 2018-09-20 Novozymes A/S Beta-Alanine Aminotransferases For The Production of 3-Hydroxypropionic Acid
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
US11268111B2 (en) * 2017-02-21 2022-03-08 Duke University Compositions and methods for robust dynamic metabolic control of a biofermentation process
US11203744B2 (en) 2018-06-21 2021-12-21 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL129722A0 (en) * 1996-11-13 2000-02-29 Du Pont Method for the production of 1,3-propanediol by recombinant organisms
US6852517B1 (en) * 1999-08-30 2005-02-08 Wisconsin Alumni Research Foundation Production of 3-hydroxypropionic acid in recombinant organisms
US7309597B2 (en) * 2002-01-18 2007-12-18 Cargill, Incorporated Alanine 2,3-Aminomutase
AU2003256480B2 (en) * 2002-07-10 2008-03-06 The Penn State Research Foundation Method for determining gene knockout strategies
US7826975B2 (en) * 2002-07-10 2010-11-02 The Penn State Research Foundation Method for redesign of microbial production systems
CA2501574C (en) * 2002-10-04 2013-12-03 E. I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
BRPI0416546A (pt) * 2003-12-04 2007-01-09 Cargill Inc produção de ácido 3-hidroxipropiÈnico usando beta-alanina/piruvato aminotransferase
WO2005093060A1 (ja) * 2004-03-26 2005-10-06 Nippon Shokubai Co., Ltd. １，３−プロパンジオール及び／又は３−ヒドロキシプロピオン酸を製造する方法
DE102005048818A1 (de) * 2005-10-10 2007-04-12 Degussa Ag Mikrobiologische Herstellung von 3-Hydroxypropionsäure
US8673601B2 (en) * 2007-01-22 2014-03-18 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20110144377A1 (en) * 2009-06-16 2011-06-16 E. I. Du Pont Nemours And Company Process for the biological production of 3-hydroxypropionic acid with high yield

Also Published As

Publication number Publication date
US20130189787A1 (en) 2013-07-25
WO2010031083A3 (en) 2010-07-08
EP2326709A4 (en) 2013-01-23
US20150072399A1 (en) 2015-03-12
WO2010031083A2 (en) 2010-03-18
EP2326709A2 (en) 2011-06-01

Similar Documents

Publication Publication Date Title
BRPI0918875A2 (pt) Método para fazer um microorganismo geneticamente modificado, microorganismo e sistema de cultura
Li et al. Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression
JP2012010715A5 (pt)
BRPI0920650A2 (pt) métodos para produzir um aldeído graxo, microrganismo geneticamente modificado, aldeído graxo
WO2010017230A3 (en) Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
JP2017517268A5 (pt)
WO2010011874A3 (en) Methods, systems and compositions for increased microorganism tolerance to and production of 3-hydroxypropionic acid (3-hp)
WO2011140386A3 (en) Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
WO2010083250A3 (en) Single-cell nucleic acid analysis
WO2010093262A8 (en) Alcohol production process
JP2015535181A5 (pt)
AR091726A1 (es) Metodo para la preparacion de 2,4-dihidroxibutirato
UA108468C2 (en) Microorganism that expresses xylose isomerase
IN2012DN00302A (pt)
WO2012088479A3 (en) Composition to overcome inhibitors in pcr and growth cultures
NZ728287A (en) Method for producing acetoin
BR112018009195A8 (pt) fermentação
WO2016050654A1 (en) Process for converting ferulic acid into vanillin
Sabet-Azad et al. Production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde by recombinant Escherichia coli co-expressing Lactobacillus reuteri propanediol utilization enzymes
WO2011037414A3 (ko) 알코올 생성능이 증가된 재조합 변이 미생물 및 이를 이용한 알코올의 제조방법
WO2013043758A3 (en) Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products
NZ700609A (en) Enzyme-altered metabolite activity
EP2663646A2 (en) Method for the preparation of nicotinic acid
WO2014041011A3 (en) Method for the production of primary amines
JP2019531758A5 (pt)

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]