BRPI0904560A2 - método para multiplicação celular em tecidos a partir de jatropha curcas - Google Patents
método para multiplicação celular em tecidos a partir de jatropha curcas Download PDFInfo
- Publication number
- BRPI0904560A2 BRPI0904560A2 BRPI0904560-0A BRPI0904560A BRPI0904560A2 BR PI0904560 A2 BRPI0904560 A2 BR PI0904560A2 BR PI0904560 A BRPI0904560 A BR PI0904560A BR PI0904560 A2 BRPI0904560 A2 BR PI0904560A2
- Authority
- BR
- Brazil
- Prior art keywords
- culture medium
- seed
- jatropha curcas
- derived
- explants
- Prior art date
Links
- 241001048891 Jatropha curcas Species 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 66
- 239000001963 growth medium Substances 0.000 claims abstract description 114
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 18
- 241000196324 Embryophyta Species 0.000 claims description 17
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 12
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 12
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 12
- 239000001110 calcium chloride Substances 0.000 claims description 12
- 235000011148 calcium chloride Nutrition 0.000 claims description 12
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 12
- 239000005556 hormone Substances 0.000 claims description 12
- 229940088597 hormone Drugs 0.000 claims description 12
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 12
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 12
- 108010059892 Cellulase Proteins 0.000 claims description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 11
- 229940106157 cellulase Drugs 0.000 claims description 11
- 229940059442 hemicellulase Drugs 0.000 claims description 11
- 108010002430 hemicellulase Proteins 0.000 claims description 11
- 108010059820 Polygalacturonase Proteins 0.000 claims description 10
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 10
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 10
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 claims description 10
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 claims description 10
- 229940023877 zeatin Drugs 0.000 claims description 10
- 239000003617 indole-3-acetic acid Substances 0.000 claims description 9
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 9
- 229960000367 inositol Drugs 0.000 claims description 9
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 7
- 239000007836 KH2PO4 Substances 0.000 claims description 7
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- 229960003495 thiamine Drugs 0.000 claims description 7
- 235000019157 thiamine Nutrition 0.000 claims description 7
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 7
- 239000011721 thiamine Substances 0.000 claims description 7
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 7
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 7
- 239000011686 zinc sulphate Substances 0.000 claims description 7
- 235000009529 zinc sulphate Nutrition 0.000 claims description 7
- 229930091371 Fructose Natural products 0.000 claims description 6
- 239000005715 Fructose Substances 0.000 claims description 6
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 6
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 6
- 229960002685 biotin Drugs 0.000 claims description 6
- 235000020958 biotin Nutrition 0.000 claims description 6
- 239000011616 biotin Substances 0.000 claims description 6
- 235000010216 calcium carbonate Nutrition 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 6
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 6
- 229960003512 nicotinic acid Drugs 0.000 claims description 6
- 235000001968 nicotinic acid Nutrition 0.000 claims description 6
- 239000011664 nicotinic acid Substances 0.000 claims description 6
- 235000010333 potassium nitrate Nutrition 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 6
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 6
- 239000011684 sodium molybdate Substances 0.000 claims description 6
- 235000015393 sodium molybdate Nutrition 0.000 claims description 6
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 5
- 229960002743 glutamine Drugs 0.000 claims description 5
- 235000004554 glutamine Nutrition 0.000 claims description 5
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 5
- 229960001669 kinetin Drugs 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000003960 organic solvent Substances 0.000 claims description 5
- 235000008160 pyridoxine Nutrition 0.000 claims description 5
- 239000011677 pyridoxine Substances 0.000 claims description 5
- 229940011671 vitamin b6 Drugs 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 4
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 229960002449 glycine Drugs 0.000 claims description 4
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 4
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 238000000527 sonication Methods 0.000 claims description 3
- 239000005971 1-naphthylacetic acid Substances 0.000 claims description 2
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 235000019198 oils Nutrition 0.000 claims 4
- QOPBEBWGSGFROG-UHFFFAOYSA-N 2-(1h-indol-2-yl)acetic acid Chemical compound C1=CC=C2NC(CC(=O)O)=CC2=C1 QOPBEBWGSGFROG-UHFFFAOYSA-N 0.000 claims 3
- 235000015112 vegetable and seed oil Nutrition 0.000 claims 1
- 239000004744 fabric Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 54
- 239000003921 oil Substances 0.000 description 35
- 239000000203 mixture Substances 0.000 description 12
- 238000011534 incubation Methods 0.000 description 7
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- 241000221089 Jatropha Species 0.000 description 4
- 239000003225 biodiesel Substances 0.000 description 4
- 244000020518 Carthamus tinctorius Species 0.000 description 3
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 3
- 244000127993 Elaeis melanococca Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- -1 veterinary Substances 0.000 description 3
- 244000144725 Amygdalus communis Species 0.000 description 2
- 235000011437 Amygdalus communis Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
- 235000001950 Elaeis guineensis Nutrition 0.000 description 2
- 235000018060 Elaeis melanococca Nutrition 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 244000020551 Helianthus annuus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 244000179886 Moringa oleifera Species 0.000 description 2
- 235000011347 Moringa oleifera Nutrition 0.000 description 2
- 240000007817 Olea europaea Species 0.000 description 2
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 235000004426 flaxseed Nutrition 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 244000233513 Brassica perviridis Species 0.000 description 1
- 235000008744 Brassica perviridis Nutrition 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001608549 Elaeis oleifera Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 235000002725 Olea europaea Nutrition 0.000 description 1
- 240000000528 Ricinus communis Species 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 244000044822 Simmondsia californica Species 0.000 description 1
- 235000004433 Simmondsia californica Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000001866 Vernicia fordii Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- YTZIISLVLINEJT-UHFFFAOYSA-N disodium dihypochlorite Chemical compound [Na+].[Na+].[O-]Cl.[O-]Cl YTZIISLVLINEJT-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000004692 intercellular junction Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CO09040167A CO6210117A1 (es) | 2009-04-21 | 2009-04-21 | Metodo para multiplicar tejido celular de jatropha curcas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0904560A2 true BRPI0904560A2 (pt) | 2011-02-08 |
Family
ID=42667890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0904560-0A BRPI0904560A2 (pt) | 2009-04-21 | 2009-11-19 | método para multiplicação celular em tecidos a partir de jatropha curcas |
Country Status (34)
| Country | Link |
|---|---|
| EP (1) | EP2275566B1 (es) |
| JP (1) | JP5818124B2 (es) |
| KR (1) | KR101521325B1 (es) |
| CN (1) | CN101870889B (es) |
| AP (1) | AP2916A (es) |
| AR (1) | AR074378A1 (es) |
| AU (1) | AU2009238335B2 (es) |
| BR (1) | BRPI0904560A2 (es) |
| CA (1) | CA2686141C (es) |
| CL (1) | CL2009002098A1 (es) |
| CO (1) | CO6210117A1 (es) |
| CR (1) | CR20110543A (es) |
| CU (1) | CU20110197A7 (es) |
| DO (1) | DOP2011000257A (es) |
| EA (1) | EA020172B1 (es) |
| EC (1) | ECSP11011238A (es) |
| EG (1) | EG26457A (es) |
| HN (1) | HN2011002181A (es) |
| IL (1) | IL214510A (es) |
| MA (1) | MA33266B1 (es) |
| MX (1) | MX2009012611A (es) |
| MY (1) | MY158131A (es) |
| NI (1) | NI201100153A (es) |
| NZ (1) | NZ581305A (es) |
| PE (1) | PE20120841A1 (es) |
| RS (1) | RS20110433A1 (es) |
| SG (1) | SG174852A1 (es) |
| SV (1) | SV2011004006A (es) |
| TN (1) | TN2011000367A1 (es) |
| TW (1) | TW201038731A (es) |
| UA (1) | UA105519C2 (es) |
| UY (1) | UY32563A (es) |
| WO (1) | WO2010122372A1 (es) |
| ZA (1) | ZA201105559B (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7772002B1 (en) * | 2009-04-21 | 2010-08-10 | Universidad De Antioquia | Method for cellular tissue multiplication from Jatropha curcas |
| CN104012212A (zh) * | 2013-03-03 | 2014-09-03 | 四川农业大学 | 一种适用于本生烟种子快速萌发的消毒方法 |
| CN103601555A (zh) * | 2013-11-27 | 2014-02-26 | 黄永成 | 绿萝无土栽培营养液 |
| CN103601557A (zh) * | 2013-11-27 | 2014-02-26 | 申燕林 | 金边富贵竹水培营养液 |
| CN103755418A (zh) * | 2013-12-30 | 2014-04-30 | 夏家志 | 一种水培观赏型植物营养液的制备方法 |
| CN104402620A (zh) * | 2014-11-27 | 2015-03-11 | 遵义市石锐花卉种植园 | 一种花肥 |
| CN110106137B (zh) * | 2019-04-17 | 2023-02-21 | 华南农业大学 | 一种辣木叶肉原生质体及其制备方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4306022A (en) * | 1980-03-11 | 1981-12-15 | Cornell Research Foundation, Inc. | Cocoa bean cell culture |
| JPH0789804B2 (ja) * | 1986-12-18 | 1995-10-04 | 三井石油化学工業株式会社 | 球根植物の増殖方法 |
| SU1761799A1 (ru) * | 1990-07-30 | 1992-09-15 | Центральный Ботанический Сад Ан Бсср | Питательна среда дл культивировани калуссной ткани бегонии краснолистной |
| GB9110855D0 (en) * | 1991-05-20 | 1991-07-10 | Zaadunie Bv | Improvements in or relating to organic compounds |
| JPH06239719A (ja) * | 1993-02-17 | 1994-08-30 | Tama Seikagaku Kk | トウダイグサ科植物の種子の抽出油、当該抽出油の製造方法、及び当該抽出油を有効成分とする化粧料 |
| RU2147608C1 (ru) * | 1998-04-07 | 2000-04-20 | Закрытое акционерное общество "Второй цех" | Штамм культивируемых клеток растений panax ginseng c.a.mey - продуцент биологически активных веществ |
| WO2006129988A1 (en) * | 2005-06-03 | 2006-12-07 | Samyang Genex Corporation | Mass production of secondary metabolite in plant cell culture by treatment of saccharide mixture in medicum |
| CN1723770A (zh) * | 2005-07-19 | 2006-01-25 | 南京九康科技发展有限公司 | 一种楝树杂交组织培养技术 |
| RU2308484C2 (ru) * | 2005-12-07 | 2007-10-20 | Иван Юрьевич Лимбах | Способ получения биомассы клеток растений |
| CN100477906C (zh) * | 2006-11-23 | 2009-04-15 | 上海光兆植物速生技术有限公司 | 采用甲基磺酸乙酯诱变木本植物的方法 |
| CN101225413A (zh) * | 2007-01-18 | 2008-07-23 | 肇东爱尔乳酸有限公司 | 一种非钙盐法自动循环连续发酵生产乳酸的方法 |
| US7932086B2 (en) | 2007-02-14 | 2011-04-26 | Reliance Life Sciences Pvt. Ltd. | Commercially viable process for in vitro mass culture of Jatropha curcas |
| JP2008278759A (ja) * | 2007-05-08 | 2008-11-20 | Hirosaki Univ | 植物組織培養を利用したｃｉｓ−イソプレノイド類の合成法 |
| CN101225416A (zh) * | 2008-02-25 | 2008-07-23 | 贵州大学 | 一种用微生物发酵法制备麻疯树籽油脂的方法 |
| GB0807619D0 (en) * | 2008-04-28 | 2008-06-04 | Whitton Peter A | Production of bio fuels from plant tissue culture sources |
-
2009
- 2009-04-21 CO CO09040167A patent/CO6210117A1/es active IP Right Grant
- 2009-11-17 CL CL2009002098A patent/CL2009002098A1/es unknown
- 2009-11-17 CA CA2686141A patent/CA2686141C/en not_active Expired - Fee Related
- 2009-11-19 NZ NZ581305A patent/NZ581305A/en not_active IP Right Cessation
- 2009-11-19 SG SG2011056785A patent/SG174852A1/en unknown
- 2009-11-19 UA UAA201112099A patent/UA105519C2/uk unknown
- 2009-11-19 MY MYPI20094924A patent/MY158131A/en unknown
- 2009-11-19 MA MA34339A patent/MA33266B1/fr unknown
- 2009-11-19 RS RS20110433A patent/RS20110433A1/sr unknown
- 2009-11-19 EP EP09275110.6A patent/EP2275566B1/en not_active Not-in-force
- 2009-11-19 BR BRPI0904560-0A patent/BRPI0904560A2/pt not_active Application Discontinuation
- 2009-11-19 AP AP2011005858A patent/AP2916A/xx active
- 2009-11-19 AR ARP090104467A patent/AR074378A1/es unknown
- 2009-11-19 KR KR1020117022014A patent/KR101521325B1/ko not_active Expired - Fee Related
- 2009-11-19 PE PE2011001639A patent/PE20120841A1/es not_active Application Discontinuation
- 2009-11-19 AU AU2009238335A patent/AU2009238335B2/en not_active Ceased
- 2009-11-19 WO PCT/IB2009/007517 patent/WO2010122372A1/en not_active Ceased
- 2009-11-20 JP JP2009265017A patent/JP5818124B2/ja not_active Expired - Fee Related
- 2009-11-20 MX MX2009012611A patent/MX2009012611A/es active IP Right Grant
- 2009-11-20 EA EA200970994A patent/EA020172B1/ru not_active IP Right Cessation
- 2009-11-20 CN CN200910223983.4A patent/CN101870889B/zh not_active Expired - Fee Related
-
2010
- 2010-04-19 TW TW099112217A patent/TW201038731A/zh unknown
- 2010-04-20 UY UY0001032563A patent/UY32563A/es not_active Application Discontinuation
-
2011
- 2011-07-26 TN TN2011000367A patent/TN2011000367A1/fr unknown
- 2011-07-26 EC EC2011011238A patent/ECSP11011238A/es unknown
- 2011-07-28 ZA ZA2011/05559A patent/ZA201105559B/en unknown
- 2011-08-05 NI NI201100153A patent/NI201100153A/es unknown
- 2011-08-08 IL IL214510A patent/IL214510A/en active IP Right Grant
- 2011-08-10 DO DO2011000257A patent/DOP2011000257A/es unknown
- 2011-08-16 HN HN2011002181A patent/HN2011002181A/es unknown
- 2011-08-19 SV SV2011004006A patent/SV2011004006A/es unknown
- 2011-10-18 CR CR20110543A patent/CR20110543A/es unknown
- 2011-10-19 EG EG2011101754A patent/EG26457A/en active
- 2011-10-21 CU CU20110197A patent/CU20110197A7/es unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BRPI0904560A2 (pt) | método para multiplicação celular em tecidos a partir de jatropha curcas | |
| CN102559376B (zh) | 一种提取异养小球藻中性油脂的方法 | |
| BRPI0911529B1 (pt) | método para a produção de pelo menos um ácido graxo e/ou óleo, meio de cultura celular vegetal em suspensão tamponado, uso do mesmo e uso de uma cultura celular de células vegetais produtoras de óleo em suspensão | |
| CN114208616B (zh) | 一种天麻的有机种植方法 | |
| KR20130048234A (ko) | 왁스에스테르 고함유 유글레나의 생산방법 및 왁스에스테르 제조방법 | |
| Suthar et al. | Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb | |
| Huang et al. | Mechanism of the allelopathic effect of macroalgae Gracilaria bailiniae on Nitzschia closterium | |
| US7772002B1 (en) | Method for cellular tissue multiplication from Jatropha curcas | |
| Nahar et al. | In vitro plant regeneration from shoot tip explants of Jatropha curcas L: A biodiesel plant | |
| Sánchez et al. | Lipids extraction from microalgae for biodiesel production | |
| Hakim et al. | The optimum yield of Nannochloropsis sp microalgae from the lipid cultivation and extraction process with Soxhlet method | |
| Nahar et al. | Jatropha curcas L. and Ricinus communis L: in vitro plant propagation from shoot tip explants for commercial cultivation and biofuel production | |
| CN103952310A (zh) | 一种快速批量靶向化筛选生物柴油微藻的方法及系统 | |
| CN1046658A (zh) | 使用合成品9-β-L(+)腺苷刺激生长的方法 | |
| HK1150059B (en) | Method for cellular tissue multiplication from jatropha curcas | |
| Bala | In vitro Seed Germination Behaviour of Simmondsia chinensis | |
| Sari et al. | Moisture Reduction on Essential Oil with Batch Distillation Process from A Polianthes Tuberos | |
| Chinweuba et al. | Synthesis and Characterization of Biodieselfrom Micro Algae (Oscillotoria Formosa) | |
| JP2545359B2 (ja) | 植物培養細胞 | |
| Shrivastava et al. | A Complete Protocol for the Native Biodiesel Plant-Pongamia Pinnata Using Low Cost Alternatives for Development of high Frequency micropropagation | |
| Tomita | Effect of Complex Supplements on Growth of Ophrys Seedlings In Vitro | |
| Kumar et al. | Explant dependent, high frequency, repetitive somatic embryogenesis and plan | |
| Boothalingam et al. | Characterization of Algal Oil from Chlorella sp., and its growth optimization using Co-solvent systems | |
| Bhargava et al. | IN VITRO REGENERATION OF JATROPHA CURCAS | |
| WO2017095212A1 (es) | Proceso biotecnológico para la detoxificación y obtención concomitante de biocarburantes/biolubricantes a partir de pastas de oleaginosas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B03A | Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL |