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BRPI0812920A2 - Sistema de expressão de celulase heteróloga e homóloga - Google Patents

Sistema de expressão de celulase heteróloga e homóloga

Info

Publication number
BRPI0812920A2
BRPI0812920A2 BRPI0812920-7A2A BRPI0812920A BRPI0812920A2 BR PI0812920 A2 BRPI0812920 A2 BR PI0812920A2 BR PI0812920 A BRPI0812920 A BR PI0812920A BR PI0812920 A2 BRPI0812920 A2 BR PI0812920A2
Authority
BR
Brazil
Prior art keywords
heterologous
expression system
cellular expression
homologous cellular
homologous
Prior art date
Application number
BRPI0812920-7A2A
Other languages
English (en)
Inventor
Benjamin S Bower
Edmund A Larenas
Original Assignee
Danisco Us Inc Genencor Div
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc Genencor Div filed Critical Danisco Us Inc Genencor Div
Publication of BRPI0812920A2 publication Critical patent/BRPI0812920A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0812920-7A2A 2007-06-08 2008-06-05 Sistema de expressão de celulase heteróloga e homóloga BRPI0812920A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93389407P 2007-06-08 2007-06-08
PCT/US2008/007077 WO2008153903A2 (en) 2007-06-08 2008-06-05 Heterologous and homologous cellulase expression system

Publications (1)

Publication Number Publication Date
BRPI0812920A2 true BRPI0812920A2 (pt) 2014-10-14

Family

ID=39767012

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0812920-7A2A BRPI0812920A2 (pt) 2007-06-08 2008-06-05 Sistema de expressão de celulase heteróloga e homóloga

Country Status (11)

Country Link
US (2) US20100323426A1 (pt)
EP (1) EP2171039A2 (pt)
JP (1) JP5651466B2 (pt)
CN (2) CN104726350A (pt)
BR (1) BRPI0812920A2 (pt)
CA (1) CA2693084A1 (pt)
EA (2) EA201300048A1 (pt)
IL (1) IL202304A (pt)
MX (2) MX344810B (pt)
UA (1) UA104279C2 (pt)
WO (1) WO2008153903A2 (pt)

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US8097445B2 (en) * 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
CN104726350A (zh) * 2007-06-08 2015-06-24 丹尼斯科美国公司 异源和同源纤维素酶表达体系
US9181537B2 (en) * 2009-07-03 2015-11-10 Meiji Seika Pharma Co., Ltd. Cellulase preparation comprising endoglucanases derived from two different types of microorganisms
EP3296394B1 (en) 2009-09-23 2020-11-04 Danisco US Inc. Novel glycosyl hydrolase enzymes and uses thereof
CN107287250A (zh) 2009-12-23 2017-10-24 丹尼斯科美国公司 提高同步糖化发酵反应效率的方法
EP2534244A1 (en) 2010-02-11 2012-12-19 DSM IP Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
US8759064B2 (en) 2010-05-14 2014-06-24 Codexis, Inc. Cellobiohydrolase variants
UA115306C2 (uk) 2011-03-17 2017-10-25 Даніско Юес Інк. Спосіб зменшення в'язкості в процесі оцукрювання
US20120276594A1 (en) * 2011-04-27 2012-11-01 Codexis, Inc. Cellobiohydrolase variants
US8945903B2 (en) 2011-08-23 2015-02-03 Codexis, Inc. Cellobiohydrolase variants
CN102304540B (zh) * 2011-08-26 2013-10-09 华东理工大学 一种在里氏木霉分泌表达外源蛋白的表达设备及其应用
BR112014006676A2 (pt) * 2011-09-20 2018-09-04 Codexis Inc endoglucanase 1b
JP6105590B2 (ja) 2011-09-22 2017-03-29 ダニスコ・ユーエス・インク Ｄｎａ含有量を減少させる内因性ｄｎアーゼ活性
WO2013177153A1 (en) 2012-05-21 2013-11-28 Danisco Us Inc. Trichoderma hydrophobin production
WO2014192647A1 (ja) * 2013-05-27 2014-12-04 独立行政法人産業技術総合研究所 培養細胞および糖液の製造方法
EP3419992B1 (en) 2016-02-22 2020-11-18 Danisco US Inc. Fungal high-level protein production system
EP4095152A3 (en) 2016-03-04 2022-12-28 Danisco US Inc. Engineered ribosomal promoters for protein production in microorganisms
US10428334B2 (en) * 2016-04-05 2019-10-01 Wisconsin Alumni Research Foundation Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates
JP7384035B2 (ja) * 2017-09-25 2023-11-21 味の素株式会社 タンパク質の製造法および二糖の製造法
JP2021035330A (ja) * 2017-09-29 2021-03-04 Ｓｐｉｂｅｒ株式会社 発現カセット
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536655A (en) * 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase
US5475101A (en) * 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5677151A (en) * 1994-06-24 1997-10-14 Cornell Research Foundation, Inc. Thermostable cellulase from a thermomonospora gene
DK0857216T3 (en) * 1995-10-17 2014-12-15 Ab Enzymes Oy Cellulases, GENES ENCODING THEM AND USES THEREOF
FI964692A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammansaettning foer bioefterbehandling av textilmaterial som innehaoller cellulosa
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US7074608B1 (en) * 1998-05-12 2006-07-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis
CA2331199C (en) * 1998-06-10 2012-10-23 Markus Sakari Kauppinen Isolated mannanases for use in treating cellulosic or synthetic fibers
US6358715B1 (en) * 1998-12-04 2002-03-19 Genencor International, Inc. Production of ascorbic acid
WO2002024882A2 (en) * 2000-09-25 2002-03-28 Iogen Energy Corporation Method for glucose production with a modified cellulase mixture
WO2004016760A2 (en) * 2002-08-16 2004-02-26 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
US7279321B2 (en) * 2003-01-17 2007-10-09 E.I. Du Pont De Nemours And Company Methods for increasing the resistance of a host cell to aromatic carboxylic acids
US7449550B2 (en) * 2003-02-27 2008-11-11 Alliance For Sustainable Energy, Llc Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
WO2004081201A1 (en) * 2003-03-12 2004-09-23 Vib Vzw Improved protein secretion in eukaryotic cells
EP2311942A1 (en) * 2003-03-21 2011-04-20 Genecor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
ES2430825T3 (es) * 2003-04-01 2013-11-21 Danisco Us Inc. CBH1.1 de Humicola grisea variante
ES2393058T3 (es) * 2003-05-02 2012-12-18 Novozymes Inc. Variantes de beta-glucosidasa
US8097445B2 (en) * 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
EP1727903B1 (en) * 2004-03-25 2011-10-05 Iogen Bio-Products Corporation Modified xylanases exhibiting improved expression
US7413887B2 (en) * 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
JP5148288B2 (ja) * 2004-12-30 2013-02-20 ジェネンコー・インターナショナル・インク 新規変異体ハイポクレアジェコリーナｃｂｈ２セルラーゼ
US8206970B2 (en) * 2006-05-02 2012-06-26 Butamax(Tm) Advanced Biofuels Llc Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
CN104726350A (zh) * 2007-06-08 2015-06-24 丹尼斯科美国公司 异源和同源纤维素酶表达体系
ZA201007564B (en) * 2008-04-23 2012-01-25 Goodyear Tire & Rubber Isoprene synthase variants for improved microbial production of isoprene
EP2601300A1 (en) * 2010-08-06 2013-06-12 Danisco US Inc. Production of isoprene under neutral ph conditions
CN103476926A (zh) * 2010-12-22 2013-12-25 丹尼斯科美国公司 使用两种类型的ispg酶改善异戊二烯产量的组合物和方法

Also Published As

Publication number Publication date
CA2693084A1 (en) 2008-12-18
IL202304A (en) 2016-08-31
IL202304A0 (en) 2011-08-01
JP5651466B2 (ja) 2015-01-14
CN104726350A (zh) 2015-06-24
UA104279C2 (uk) 2014-01-27
EP2171039A2 (en) 2010-04-07
EA018049B1 (ru) 2013-05-30
WO2008153903A2 (en) 2008-12-18
WO2008153903A3 (en) 2009-03-05
US20100184138A1 (en) 2010-07-22
CN101772570A (zh) 2010-07-07
MX344810B (es) 2017-01-09
MX2009013329A (es) 2010-02-24
EA200971142A1 (ru) 2010-04-30
JP2010528655A (ja) 2010-08-26
HK1145851A1 (en) 2011-05-06
EA201300048A1 (ru) 2013-09-30
US20100323426A1 (en) 2010-12-23
CN101772570B (zh) 2015-03-18

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]