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BR9914299A - Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas - Google Patents

Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas

Info

Publication number
BR9914299A
BR9914299A BR9914299-6A BR9914299A BR9914299A BR 9914299 A BR9914299 A BR 9914299A BR 9914299 A BR9914299 A BR 9914299A BR 9914299 A BR9914299 A BR 9914299A
Authority
BR
Brazil
Prior art keywords
new
amylase
stimulation
nucleic acid
target
Prior art date
Application number
BR9914299-6A
Other languages
English (en)
Inventor
Thomas Anthony Kavanagh
Nga Thi Lao
Original Assignee
Cambridge Advanced Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9817959.1A external-priority patent/GB9817959D0/en
Priority claimed from GBGB9817963.3A external-priority patent/GB9817963D0/en
Priority claimed from GBGB9913014.8A external-priority patent/GB9913014D0/en
Application filed by Cambridge Advanced Tech filed Critical Cambridge Advanced Tech
Publication of BR9914299A publication Critical patent/BR9914299A/pt

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"UMA NOVA SEQUêNCIA DE áCIDO NUCLéICO DE UMPLASTìDIO-ALVO, UMA NOVA SEQuêNCIA <225>-AMILASE,UM PROMOTOR SUSCEPTìVEL A ESTIMULO E USO DASMESMAS". A invenção provê um novo cloroplasto com uma novaseq³ência de <255>-amilase alvo (ct-<255>-amilase), uma novaseq³ência de ácido nucléico alvo do cloroplasto e uma novaseq³ência de <255>-amilase. Também é descrito umpromotor-indutor susceptìvel independentemente de estímulos deluz ou de açúcar. Métodos de transformação das plantas atravésdo uso de ditas seq³ências também são descritos, bem como ascélulas transformadas das plantas, plantas transformadas, esementes de ditas plantas transformadas, e ainda genes quiméricoscontendo as referidas seq³ências. A modificação do nível deamido nas plantas pode ser conseguida, bem como a marcaçãodos genes através da degradação ou da biosíntese do amido,doenças ou resistência a pesticidas ou variação da expressão dogene devido a estímulo são descritas.
BR9914299-6A 1998-08-19 1999-08-13 Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas BR9914299A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9817959.1A GB9817959D0 (en) 1998-08-19 1998-08-19 A novel plastid-targeting nucleic acid sequence and a novel B-amylase sequence and uses therefor
GBGB9817963.3A GB9817963D0 (en) 1998-08-19 1998-08-19 A plastid-targeting nucleic acid sequence and uses therefor
GBGB9913014.8A GB9913014D0 (en) 1999-06-05 1999-06-05 A plastid-targeting nucleic acid sequence,a stimulus-responsive promoter,and uses thereof
PCT/GB1999/002697 WO2000011144A2 (en) 1998-08-19 1999-08-13 Plastid-targeting nucleic acid sequence, beta-amylase sequence, a stimulus-responsive promoter and uses thereof

Publications (1)

Publication Number Publication Date
BR9914299A true BR9914299A (pt) 2001-11-27

Family

ID=27269441

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9914299-6A BR9914299A (pt) 1998-08-19 1999-08-13 Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas

Country Status (18)

Country Link
US (2) US6489540B1 (pt)
EP (1) EP1105468B1 (pt)
JP (2) JP2002523040A (pt)
CN (2) CN1528904B (pt)
AT (1) ATE527346T1 (pt)
AU (1) AU773492B2 (pt)
BR (1) BR9914299A (pt)
CA (1) CA2336249C (pt)
CZ (1) CZ303574B6 (pt)
ES (1) ES2375034T3 (pt)
HU (1) HU228696B1 (pt)
MX (1) MXPA01001815A (pt)
NZ (1) NZ509642A (pt)
PL (1) PL205558B1 (pt)
SK (1) SK288125B6 (pt)
TR (1) TR200100625T2 (pt)
WO (1) WO2000011144A2 (pt)
ZA (1) ZA200100736B (pt)

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JP3546337B2 (ja) * 1993-12-21 2004-07-28 ゼロックス コーポレイション 計算システム用ユーザ・インタフェース装置及びグラフィック・キーボード使用方法
BR9914299A (pt) 1998-08-19 2001-11-27 Cambridge Advanced Tech Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas
CA2438388A1 (en) 2001-02-22 2002-09-06 Pioneer Hi-Bred International, Inc. Manipulation of sucrose synthase genes to improve stalk and grain quality
EP1381676A2 (en) * 2001-04-25 2004-01-21 Max-Planck-Gesellschaft Zur Förderung Der Wissenschaften E.V. Nucleic acid molecules encoding starch degrading enzymes
RU2312144C2 (ru) * 2001-08-27 2007-12-10 Синджента Партисипейшнс Аг Аутопроцессирующиеся растения и части растений
US20060233930A1 (en) * 2002-11-08 2006-10-19 Bayer Cropscience Gmbh Process for reducing the acrylamide content of heat-treated foods
JP2007151435A (ja) * 2005-12-02 2007-06-21 Niigata Univ デンプン集積能力の高い形質転換植物およびその製造方法
MX2010004960A (es) * 2007-11-05 2010-05-20 Syngenta Participations Ag Metodos para incrementar el contenido de almidon en las plantas.
WO2010099134A1 (en) * 2009-02-25 2010-09-02 Syngenta Participations Ag Methods for increasing starch content in plant cobs
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
WO2011163659A2 (en) 2010-06-25 2011-12-29 Agrivida, Inc. Plants with altered levels of vegetative starch
WO2015021600A1 (en) * 2013-08-13 2015-02-19 Danisco Us Inc. Beta-amylase and methods of use
CN104232681B (zh) * 2014-09-28 2017-02-15 浙江大学 一种植物表达载体及在制备磷酸化改性水稻淀粉中的应用
CN117481005B (zh) * 2023-09-21 2024-08-02 中国烟草总公司广东省公司 一种坡地烟与山旱稻双优融合的种植方法

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BR9914299A (pt) * 1998-08-19 2001-11-27 Cambridge Advanced Tech Uma nova sequência de ácido nucléico de umplastìdio-alvo, uma nova sequência ß-amilase, umpromotor susceptìvel a estìmulo e uso das mesmas

Also Published As

Publication number Publication date
SK2042001A3 (en) 2002-01-07
ATE527346T1 (de) 2011-10-15
CN1323349A (zh) 2001-11-21
AU773492B2 (en) 2004-05-27
HU228696B1 (en) 2013-05-28
JP2002523040A (ja) 2002-07-30
EP1105468A2 (en) 2001-06-13
JP4673431B2 (ja) 2011-04-20
WO2000011144A2 (en) 2000-03-02
US6489540B1 (en) 2002-12-03
CZ2001460A3 (cs) 2001-11-14
WO2000011144A3 (en) 2000-09-08
CA2336249C (en) 2011-03-08
NZ509642A (en) 2003-08-29
MXPA01001815A (es) 2002-06-21
HUP0200732A2 (hu) 2002-07-29
SK288125B6 (sk) 2013-10-02
PL346704A1 (en) 2002-02-25
CA2336249A1 (en) 2000-03-02
HUP0200732A3 (en) 2004-10-28
ZA200100736B (en) 2002-06-26
US20030074690A1 (en) 2003-04-17
CZ303574B6 (cs) 2012-12-19
PL205558B1 (pl) 2010-05-31
CN1528904A (zh) 2004-09-15
JP2010131029A (ja) 2010-06-17
TR200100625T2 (tr) 2001-06-21
CN1234868C (zh) 2006-01-04
ES2375034T3 (es) 2012-02-24
AU5432699A (en) 2000-03-14
EP1105468B1 (en) 2011-10-05
CN1528904B (zh) 2010-05-26

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