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BR9914099A - Teste de atividade in vitro para polimerase de dna do vìrus de hepatite b humana (hbv), e seu uso para triar inibidores de polimerase de dna de hbv - Google Patents

Teste de atividade in vitro para polimerase de dna do vìrus de hepatite b humana (hbv), e seu uso para triar inibidores de polimerase de dna de hbv

Info

Publication number
BR9914099A
BR9914099A BR9914099-3A BR9914099A BR9914099A BR 9914099 A BR9914099 A BR 9914099A BR 9914099 A BR9914099 A BR 9914099A BR 9914099 A BR9914099 A BR 9914099A
Authority
BR
Brazil
Prior art keywords
hbv
dna polymerase
virus
screen
human hepatitis
Prior art date
Application number
BR9914099-3A
Other languages
English (en)
Inventor
Chong Jin Con
Wei Ning Chen
Gek Keow Lim
Ai Lin Leong
Original Assignee
Government Republic Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Government Republic Singapore filed Critical Government Republic Singapore
Publication of BR9914099A publication Critical patent/BR9914099A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Patente de Invenção: "<B>TESTE DE ATIVIDADE IN VITRO PARA POLIMERASE DE DNA DO VìRUS DE HEPATITE B HUMANO (HBV), E SEU USO PARA TRIAR INIBIDORES DE POLIMERASE DE DNA DE HBV"<D>. A presente invenção provê um ensaio de atividade in vitro para polimerase de DNA do virús de hepatite B humana (HBV), que compreende usar, como o oligonucleotídeo 5' em amplificação PCR de polimerase de DNA de HBV de uma amostra, um oligonucleotídeo em que é incorporado o promotor de polimerase viral SP6, traduzindo e transcrevendo diretamente o produto PCR na presença de um agente radiorrotulado e medindo a atividade iniciadora da polimerase de DNA de HBV. A presente invenção provê também o uso deste ensaio para testar atividade de várias amostras de soro, triar inibidores de polimerase de DNA de HBV e testar e/ou triar drogas anti- HBV potenciais para sua capacidade de inibir atividade iniciadora de DNA de polimerase de DNA de HBV humano.
BR9914099-3A 1998-09-29 1999-03-13 Teste de atividade in vitro para polimerase de dna do vìrus de hepatite b humana (hbv), e seu uso para triar inibidores de polimerase de dna de hbv BR9914099A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/SG1998/000077 WO2000018958A2 (en) 1998-09-29 1998-09-29 An in vitro activity assay for human hepatits b virus (hbv) dna polymerase, the use of such an assay to assay activity of various serum samples and to screen for inhibitors of the hbv dna polymerase and a method of producing hbv dna polymerase
PCT/SG1999/000017 WO2000018968A1 (en) 1998-09-29 1999-03-13 An in vitro activity assay for human hepatitis b virus (hbv) dna polymerase, and its use for screening for inhibitors of hbv dna polymerase

Publications (1)

Publication Number Publication Date
BR9914099A true BR9914099A (pt) 2001-07-31

Family

ID=20429877

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9914099-3A BR9914099A (pt) 1998-09-29 1999-03-13 Teste de atividade in vitro para polimerase de dna do vìrus de hepatite b humana (hbv), e seu uso para triar inibidores de polimerase de dna de hbv

Country Status (23)

Country Link
US (1) US6593082B1 (pt)
EP (1) EP1117843A1 (pt)
JP (1) JP2002525130A (pt)
KR (1) KR100445958B1 (pt)
CN (1) CN1197978C (pt)
AR (1) AR030012A1 (pt)
AU (2) AU9372698A (pt)
BR (1) BR9914099A (pt)
CA (1) CA2344282A1 (pt)
HK (1) HK1039161A1 (pt)
HR (1) HRP20010230B1 (pt)
HU (1) HUP0103735A3 (pt)
IL (2) IL142288A0 (pt)
IS (1) IS5900A (pt)
MY (1) MY123274A (pt)
NO (1) NO20011510L (pt)
NZ (1) NZ510704A (pt)
PL (1) PL347026A1 (pt)
RU (1) RU2202620C2 (pt)
TR (1) TR200100899T2 (pt)
UA (1) UA75028C2 (pt)
WO (2) WO2000018958A2 (pt)
YU (1) YU23601A (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7893501A (en) * 2000-07-14 2002-01-30 Replidyne Inc Novel dna polymerase iii holoenzyme delta subunit nucleic acid molecules and proteins
AUPS037002A0 (en) * 2002-02-07 2002-02-28 Melbourne Health Viral variants and uses therefor
KR20030075638A (ko) * 2002-03-20 2003-09-26 (주) 비엔씨바이오팜 Ｂ형 간염바이러스의 프라이밍 저해제의 스크리닝 방법
TW200643171A (en) 2005-06-07 2006-12-16 Temasek Life Sciences Lab Ltd Porcine circovirus type 2 vaccines
WO2008097926A2 (en) 2007-02-02 2008-08-14 Yale University Transient transfection with rna
US10155038B2 (en) 2007-02-02 2018-12-18 Yale University Cells prepared by transient transfection and methods of use thereof
US9249423B2 (en) 2007-02-02 2016-02-02 Yale University Method of de-differentiating and re-differentiating somatic cells using RNA
AU2010306639B2 (en) 2009-10-16 2017-02-16 Glaxo Group Limited HBV antisense inhibitors
CN104316682B (zh) * 2014-10-10 2015-12-30 三峡大学 一种s-腺苷甲硫氨酸脱羧酶的活性检测方法及其应用
RU2763173C1 (ru) * 2020-07-27 2021-12-28 Федеральное бюджетное учреждение науки "Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" (ФБУН НИИ эпидемиологии и микробиологии имени Пастера) Способ выявления ДНК вируса гепатита В в биологическом материале при низкой вирусной нагрузке на основе двухэтапной ПЦР с детекцией по трем мишеням в режиме реального времени

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5492817A (en) 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5360714A (en) * 1992-08-28 1994-11-01 Fox Chase Cancer Center Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof
AU4843893A (en) * 1992-09-02 1994-03-29 Scripps Research Institute, The Coupled isothermal polynucleotide amplification and translation system
US5709998A (en) * 1993-12-15 1998-01-20 The Johns Hopkins University Molecular diagnosis of familial adenomatous polyposis
RU2082759C1 (ru) * 1994-08-22 1997-06-27 Центр "Биоинженерия" Ран Штамм дрожжей saccharomyces cerevisial, содержащий рекомбинантную плазмиду yep 63/ab, - продуцент производного м-белка вируса гепатита в человека
WO1996010649A1 (en) * 1994-09-30 1996-04-11 Southwest Foundation For Biomedical Research Isolated human hepatitis b virus polymerase and uses thereof

Also Published As

Publication number Publication date
WO2000018968A8 (en) 2002-11-07
MY123274A (en) 2006-05-31
HRP20010230A2 (en) 2002-06-30
US6593082B1 (en) 2003-07-15
IS5900A (is) 2001-03-19
HUP0103735A3 (en) 2005-12-28
AU9372698A (en) 2000-04-17
CN1197978C (zh) 2005-04-20
YU23601A (sh) 2005-07-19
EP1117843A1 (en) 2001-07-25
KR100445958B1 (ko) 2004-08-25
JP2002525130A (ja) 2002-08-13
AU772819B2 (en) 2004-05-06
HK1039161A1 (zh) 2002-04-12
TR200100899T2 (tr) 2001-07-23
NO20011510L (no) 2001-05-28
AU2969499A (en) 2000-04-17
WO2000018968A1 (en) 2000-04-06
NZ510704A (en) 2002-10-25
UA75028C2 (en) 2006-03-15
WO2000018958A2 (en) 2000-04-06
PL347026A1 (en) 2002-03-11
CN1320167A (zh) 2001-10-31
IL142288A0 (en) 2002-03-10
HUP0103735A2 (hu) 2002-01-28
IL142288A (en) 2006-12-31
RU2202620C2 (ru) 2003-04-20
NO20011510D0 (no) 2001-03-23
KR20010083879A (ko) 2001-09-03
AR030012A1 (es) 2003-08-13
HRP20010230B1 (en) 2006-05-31
CA2344282A1 (en) 2000-04-06

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AO NAO RECOLHIMENTO DA 9A E 10A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 1999 DE 28/04/2009.