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BR9913644A - Método de realização de transgênese - Google Patents

Método de realização de transgênese

Info

Publication number
BR9913644A
BR9913644A BR9913644-9A BR9913644A BR9913644A BR 9913644 A BR9913644 A BR 9913644A BR 9913644 A BR9913644 A BR 9913644A BR 9913644 A BR9913644 A BR 9913644A
Authority
BR
Brazil
Prior art keywords
cells
offspring
microinjection
unfertilized
oocyte
Prior art date
Application number
BR9913644-9A
Other languages
English (en)
Inventor
Anthony C F Perry
Teruhiko Wakayama
Original Assignee
Anthony C F Perry
Teruhiko Wakayama
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anthony C F Perry, Teruhiko Wakayama filed Critical Anthony C F Perry
Publication of BR9913644A publication Critical patent/BR9913644A/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • A01K67/63—Genetically modified worms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • A01K67/65—Genetically modified arthropods
    • A01K67/67—Genetically modified crustaceans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Patente de Invenção: "MéTODO DE REALIZAçãO DETRANSGêNESE". A invenção provê um método para a geraçãode animais e células transgênicos através da co-inserção de ácidonucléico e um núcleo em um oócito não-fertilizado. De preferência,a co-inserção é através de microinjeção e com mais preferênciaatravés de microinjeção piezoeletricamente atuada. Os embriõesde expressão de transgene (tg) são aqui produzidos seguindo aco-injeção de oócitos de camundongos não-fertilizados comcabeças de espermatozóide e DNA exógeno codificando ou umaproteína verde fluorescente (GFP) ou um repórter ß-galactosidase.O oócito microinjetado pode ser deixado desenvolver em célulasdiferenciadas ou células de origem; em um embrião in vitro antesda transferência a uma mãe hospedeira substituta, ou ele pode sertransferido diretamente para uma mãe hospedeira substituta. Odesenvolvimento embriónico pode ocorrer até o final, de modo quea prole possua modificações transgênicas que possam alterar assuas características (fenótipo) e são, por sua vez, transmitidas paraa prole.
BR9913644-9A 1998-08-11 1999-08-11 Método de realização de transgênese BR9913644A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9607898P 1998-08-11 1998-08-11
US13425199P 1999-05-13 1999-05-13
PCT/US1999/018429 WO2000008924A1 (en) 1998-08-11 1999-08-11 Method of performing transgenesis

Publications (1)

Publication Number Publication Date
BR9913644A true BR9913644A (pt) 2001-11-20

Family

ID=26791041

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9913644-9A BR9913644A (pt) 1998-08-11 1999-08-11 Método de realização de transgênese

Country Status (9)

Country Link
EP (1) EP1111991A4 (pt)
JP (1) JP2002524054A (pt)
CN (1) CN1316878A (pt)
BR (1) BR9913644A (pt)
CA (1) CA2340242A1 (pt)
IL (1) IL141362A0 (pt)
MX (1) MXPA01001586A (pt)
NZ (1) NZ509861A (pt)
WO (1) WO2000008924A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2340199A1 (en) 1998-08-11 2000-02-24 University Of Hawaii Mammalian transgenesis by intracytoplasmic sperm injection
AU2103601A (en) * 1999-12-17 2001-06-25 Oregon Health And Science University Methods for producing transgenic animals
US7053187B2 (en) 2000-03-28 2006-05-30 Gioagri Corporation Sperm-specific monoclonal antibody, mAbC
US7067308B1 (en) * 2000-03-28 2006-06-27 Bioagri Corporation Vector for genetically modifying non-human animals
US6846306B1 (en) * 2000-10-10 2005-01-25 Cold Spring Harbor Laboratory Single cell electroporation
JP4159358B2 (ja) * 2000-12-27 2008-10-01 アクシオジェネシス アーゲー 分化中の胚幹細胞、成体幹細胞および胚生殖系細胞を細胞特異的および成長特異的に選択するためのシステム
CN101407807B (zh) * 2008-11-19 2013-11-06 暨南大学 对染色体进行处理而改变细胞遗传特性的方法及其应用
FR2969497B1 (fr) * 2010-12-27 2013-06-28 Ceva Sante Animale Composition luminescente comme biomarqueur dans un oeuf aviaire, dispositif et procede correspondants.
CN104372016A (zh) * 2013-10-19 2015-02-25 刘立新 在受体细胞的目的基因组序列引入变异的系统和方法
CN104911212A (zh) * 2015-05-27 2015-09-16 中国科学院广州生物医药与健康研究院 一种高效转染HaCaT细胞的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6641526B1 (en) * 1998-03-20 2003-11-04 University Of Hawaii Development of normal offspring from oocytes injected with freeze-dried spermatozoa

Also Published As

Publication number Publication date
WO2000008924A1 (en) 2000-02-24
CN1316878A (zh) 2001-10-10
EP1111991A1 (en) 2001-07-04
NZ509861A (en) 2004-01-30
JP2002524054A (ja) 2002-08-06
IL141362A0 (en) 2002-03-10
EP1111991A4 (en) 2004-03-10
WO2000008924A9 (en) 2000-05-25
CA2340242A1 (en) 2000-02-24
MXPA01001586A (es) 2002-04-08

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A,7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1933 DE 22/01/2008.