BR9913644A - Method of carrying out transgenesis - Google Patents
Method of carrying out transgenesisInfo
- Publication number
- BR9913644A BR9913644A BR9913644-9A BR9913644A BR9913644A BR 9913644 A BR9913644 A BR 9913644A BR 9913644 A BR9913644 A BR 9913644A BR 9913644 A BR9913644 A BR 9913644A
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- offspring
- microinjection
- unfertilized
- oocyte
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 210000000287 oocyte Anatomy 0.000 abstract 3
- 238000003780 insertion Methods 0.000 abstract 2
- 238000000520 microinjection Methods 0.000 abstract 2
- 230000009261 transgenic effect Effects 0.000 abstract 2
- 108091029865 Exogenous DNA Proteins 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 abstract 1
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 102000005936 beta-Galactosidase Human genes 0.000 abstract 1
- 108010005774 beta-Galactosidase Proteins 0.000 abstract 1
- 230000013020 embryo development Effects 0.000 abstract 1
- 210000002257 embryonic structure Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 210000001161 mammalian embryo Anatomy 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000012546 transfer Methods 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/60—New or modified breeds of invertebrates
- A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
- A01K67/63—Genetically modified worms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/60—New or modified breeds of invertebrates
- A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
- A01K67/65—Genetically modified arthropods
- A01K67/67—Genetically modified crustaceans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Patente de Invenção: "MéTODO DE REALIZAçãO DETRANSGêNESE". A invenção provê um método para a geraçãode animais e células transgênicos através da co-inserção de ácidonucléico e um núcleo em um oócito não-fertilizado. De preferência,a co-inserção é através de microinjeção e com mais preferênciaatravés de microinjeção piezoeletricamente atuada. Os embriõesde expressão de transgene (tg) são aqui produzidos seguindo aco-injeção de oócitos de camundongos não-fertilizados comcabeças de espermatozóide e DNA exógeno codificando ou umaproteína verde fluorescente (GFP) ou um repórter ß-galactosidase.O oócito microinjetado pode ser deixado desenvolver em célulasdiferenciadas ou células de origem; em um embrião in vitro antesda transferência a uma mãe hospedeira substituta, ou ele pode sertransferido diretamente para uma mãe hospedeira substituta. Odesenvolvimento embriónico pode ocorrer até o final, de modo quea prole possua modificações transgênicas que possam alterar assuas características (fenótipo) e são, por sua vez, transmitidas paraa prole.Invention Patent: "METHOD OF PERFORMING DETRANSGENESIS". The invention provides a method for the generation of animals and transgenic cells through the co-insertion of nucleic acid and a nucleus in an unfertilized oocyte. Preferably, co-insertion is through microinjection and more preferably through piezoelectrically operated microinjection. Transgene expression (tg) embryos are produced here following the co-injection of unfertilized mice oocytes with sperm heads and exogenous DNA encoding either a fluorescent green protein (GFP) or a ß-galactosidase reporter. The microinjected oocyte can be allowed to develop in differentiated cells or cells of origin; in an in vitro embryo prior to transfer to a surrogate host mother, or it can be transferred directly to a surrogate host mother. Embryonic development can occur until the end, so that the offspring has transgenic modifications that can alter their characteristics (phenotype) and are, in turn, transmitted to the offspring.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9607898P | 1998-08-11 | 1998-08-11 | |
| US13425199P | 1999-05-13 | 1999-05-13 | |
| PCT/US1999/018429 WO2000008924A1 (en) | 1998-08-11 | 1999-08-11 | Method of performing transgenesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9913644A true BR9913644A (en) | 2001-11-20 |
Family
ID=26791041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9913644-9A BR9913644A (en) | 1998-08-11 | 1999-08-11 | Method of carrying out transgenesis |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1111991A4 (en) |
| JP (1) | JP2002524054A (en) |
| CN (1) | CN1316878A (en) |
| BR (1) | BR9913644A (en) |
| CA (1) | CA2340242A1 (en) |
| IL (1) | IL141362A0 (en) |
| MX (1) | MXPA01001586A (en) |
| NZ (1) | NZ509861A (en) |
| WO (1) | WO2000008924A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2340199A1 (en) | 1998-08-11 | 2000-02-24 | University Of Hawaii | Mammalian transgenesis by intracytoplasmic sperm injection |
| AU2103601A (en) * | 1999-12-17 | 2001-06-25 | Oregon Health And Science University | Methods for producing transgenic animals |
| US7053187B2 (en) | 2000-03-28 | 2006-05-30 | Gioagri Corporation | Sperm-specific monoclonal antibody, mAbC |
| US7067308B1 (en) * | 2000-03-28 | 2006-06-27 | Bioagri Corporation | Vector for genetically modifying non-human animals |
| US6846306B1 (en) * | 2000-10-10 | 2005-01-25 | Cold Spring Harbor Laboratory | Single cell electroporation |
| JP4159358B2 (en) * | 2000-12-27 | 2008-10-01 | アクシオジェネシス アーゲー | A system for cell-specific and growth-specific selection of differentiating embryonic stem cells, adult stem cells and embryonic germline cells |
| CN101407807B (en) * | 2008-11-19 | 2013-11-06 | 暨南大学 | Method for modifying cell inherited characteristic by processing chromosome and use thereof |
| FR2969497B1 (en) * | 2010-12-27 | 2013-06-28 | Ceva Sante Animale | LUMINESCENT COMPOSITION AS A BIOMARKER IN AN AVIAN EGG, CORRESPONDING DEVICE AND METHOD. |
| CN104372016A (en) * | 2013-10-19 | 2015-02-25 | 刘立新 | System and method for introducing variation in target genomic sequences of recipient cells |
| CN104911212A (en) * | 2015-05-27 | 2015-09-16 | 中国科学院广州生物医药与健康研究院 | Efficient HaCaT cell transfection method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| US5905042A (en) * | 1996-04-01 | 1999-05-18 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Cultured inner cell mass cell lines derived from bovine or porcine embryos |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US6641526B1 (en) * | 1998-03-20 | 2003-11-04 | University Of Hawaii | Development of normal offspring from oocytes injected with freeze-dried spermatozoa |
-
1999
- 1999-08-11 NZ NZ509861A patent/NZ509861A/en unknown
- 1999-08-11 EP EP99942164A patent/EP1111991A4/en not_active Withdrawn
- 1999-08-11 BR BR9913644-9A patent/BR9913644A/en not_active IP Right Cessation
- 1999-08-11 CA CA002340242A patent/CA2340242A1/en not_active Abandoned
- 1999-08-11 CN CN99810645.3A patent/CN1316878A/en active Pending
- 1999-08-11 JP JP2000564438A patent/JP2002524054A/en active Pending
- 1999-08-11 MX MXPA01001586A patent/MXPA01001586A/en unknown
- 1999-08-11 IL IL14136299A patent/IL141362A0/en unknown
- 1999-08-11 WO PCT/US1999/018429 patent/WO2000008924A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000008924A1 (en) | 2000-02-24 |
| CN1316878A (en) | 2001-10-10 |
| EP1111991A1 (en) | 2001-07-04 |
| NZ509861A (en) | 2004-01-30 |
| JP2002524054A (en) | 2002-08-06 |
| IL141362A0 (en) | 2002-03-10 |
| EP1111991A4 (en) | 2004-03-10 |
| WO2000008924A9 (en) | 2000-05-25 |
| CA2340242A1 (en) | 2000-02-24 |
| MXPA01001586A (en) | 2002-04-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A,7A E 8A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1933 DE 22/01/2008. |