BR9911074A - Coding genes for tomato ß-galactosidase polypeptides - Google Patents
Coding genes for tomato ß-galactosidase polypeptidesInfo
- Publication number
- BR9911074A BR9911074A BR9911074-1A BR9911074A BR9911074A BR 9911074 A BR9911074 A BR 9911074A BR 9911074 A BR9911074 A BR 9911074A BR 9911074 A BR9911074 A BR 9911074A
- Authority
- BR
- Brazil
- Prior art keywords
- galactosidase
- present
- dna
- genes
- plants
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title 1
- 240000003768 Solanum lycopersicum Species 0.000 title 1
- 102000005936 beta-Galactosidase Human genes 0.000 title 1
- 108010005774 beta-Galactosidase Proteins 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 239000013598 vector Substances 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
"GENES CODIFICADORES PARA POLIPETìDIOS DE <255>-GALACTOSIDASE DE TOMATES". A presente invenção fornece uma nova família de genes de <255>-galactosidase e seq³ências de DNA derivadas da clonagem de produtos codificadores de cDNAs desses genes, conforme exemplificado por uma proteína de <255>-galactosidase II, que é codificada por um clone de cDNA, pZBG-1-4. Um método para modificação do metabolismo da parede celular que envolve a modificação da atividade de pelo menos uma <255>-galactosidase e, portanto, modificando a qualidade do fruto é também fornecido. Também fornecido pela presente invenção é uma construção de DNA incluindo alguma ou toda a seq³ência de DNA de <255>-galactosídase, sob controle de uma região de iniciação transcricional operativa em plantas, de modo que a construção pode gerar DNA e, opcionalmente, polipetídio de <255>-galactosidase nas células das plantas. A presente invenção também se refere a vetores recombinantes, que incluem moléculas de ácido nucléico isoladas da presente invenção, e a células hospedeiras contendo os vetores recombinantes, assim como a métodos de produzir tais vetores e células hospedeiras e para uso dos mesmos para produção de polipetídios ou peptídios de <255>-galactosidase por técnicas recombinantes. A presente invenção também fornece células de plantas contendo construções de DNA da presente invenção; plantas derivadas das mesmas tendo expressão de gene de <255>-galactosidase modificada e sementes produzidas a partir de tais plantas."ENCODING GENES FOR TOMATOES <255> -GALACTOSIDASE POLYPETHYDES". The present invention provides a new family of <255> -galactosidase genes and DNA sequences derived from the cloning of products encoding cDNAs from these genes, as exemplified by a <255> -galactosidase II protein, which is encoded by a clone of cDNA, pZBG-1-4. A method for modifying cell wall metabolism that involves modifying the activity of at least one <255> -galactosidase and therefore modifying the quality of the fruit is also provided. Also provided by the present invention is a DNA construct including some or all of the <255> -galactosidase DNA sequence, under control of a transcriptional initiation region operative in plants, so that the construct can generate DNA and, optionally, polypeptide. of <255> -galactosidase in plant cells. The present invention also relates to recombinant vectors, which include isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as methods of producing such vectors and host cells and for use in them for the production of polypeptides. or <255> -galactosidase peptides by recombinant techniques. The present invention also provides plant cells containing DNA constructs of the present invention; plants derived therefrom having modified β-galactosidase gene expression and seeds produced from such plants.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8880598P | 1998-06-09 | 1998-06-09 | |
| PCT/US1999/012697 WO1999064564A1 (en) | 1998-06-09 | 1999-06-08 | GENES CODING FOR TOMATO β-GALACTOSIDASE POLYPEPTIDES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9911074A true BR9911074A (en) | 2002-01-02 |
Family
ID=22213590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9911074-1A BR9911074A (en) | 1998-06-09 | 1999-06-08 | Coding genes for tomato ß-galactosidase polypeptides |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP1084228A1 (en) |
| JP (1) | JP2002517226A (en) |
| CN (1) | CN1311816A (en) |
| AU (1) | AU778691B2 (en) |
| BR (1) | BR9911074A (en) |
| CA (1) | CA2330233A1 (en) |
| CZ (1) | CZ20004594A3 (en) |
| HU (1) | HUP0102605A2 (en) |
| MX (1) | MXPA00012210A (en) |
| NZ (1) | NZ508968A (en) |
| PL (1) | PL345269A1 (en) |
| WO (1) | WO1999064564A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT102511B (en) * | 2000-08-22 | 2007-08-01 | Inst De Ciencia Aplic E Tecnol | B-GALACTOSIDASE ENCODING GENES, PECTINAMETHYSTERASE, POLIGALACTURONASE PEX ISOLATED EEXPANSINS |
| CN100370029C (en) * | 2005-10-11 | 2008-02-20 | 山东大学 | A high-efficiency transglycosyl β-galactosidase gene |
| GB0601901D0 (en) * | 2006-01-31 | 2006-03-08 | Product and Process | |
| GB0606112D0 (en) * | 2006-03-28 | 2006-05-03 | Product and process | |
| CN114457097A (en) * | 2021-12-27 | 2022-05-10 | 河南农业大学 | Apple β-galactosidase gene Mdβ-gal18 and its application in regulating fruit ripening and softening |
| CN115807029B (en) * | 2022-11-23 | 2025-05-20 | 广东省农业科学院农业生物基因研究中心 | Application of rice OsBGAL gene in regulating seed germination |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9320930D0 (en) * | 1993-10-12 | 1993-12-01 | Zeneca Ltd | Modified fruit |
| GB9403423D0 (en) * | 1994-02-23 | 1994-04-13 | Unilever Plc | Novel exo-(1-4)- beta-D galactanase |
-
1999
- 1999-06-08 HU HU0102605A patent/HUP0102605A2/en unknown
- 1999-06-08 AU AU45498/99A patent/AU778691B2/en not_active Ceased
- 1999-06-08 MX MXPA00012210A patent/MXPA00012210A/en unknown
- 1999-06-08 BR BR9911074-1A patent/BR9911074A/en not_active Application Discontinuation
- 1999-06-08 PL PL99345269A patent/PL345269A1/en not_active Application Discontinuation
- 1999-06-08 NZ NZ508968A patent/NZ508968A/en unknown
- 1999-06-08 JP JP2000553554A patent/JP2002517226A/en active Pending
- 1999-06-08 WO PCT/US1999/012697 patent/WO1999064564A1/en not_active Ceased
- 1999-06-08 EP EP99928433A patent/EP1084228A1/en not_active Withdrawn
- 1999-06-08 CA CA002330233A patent/CA2330233A1/en not_active Abandoned
- 1999-06-08 CN CN99809249.5A patent/CN1311816A/en active Pending
- 1999-06-08 CZ CZ20004594A patent/CZ20004594A3/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002517226A (en) | 2002-06-18 |
| HUP0102605A2 (en) | 2001-11-28 |
| CN1311816A (en) | 2001-09-05 |
| MXPA00012210A (en) | 2002-10-17 |
| NZ508968A (en) | 2003-10-31 |
| CZ20004594A3 (en) | 2002-05-15 |
| AU4549899A (en) | 1999-12-30 |
| CA2330233A1 (en) | 1999-12-16 |
| EP1084228A1 (en) | 2001-03-21 |
| WO1999064564A1 (en) | 1999-12-16 |
| PL345269A1 (en) | 2001-12-03 |
| AU778691B2 (en) | 2004-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA10 | Dismissal: dismissal - article 33 of industrial property law | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |