[go: up one dir, main page]

MX9206979A - Metodo de clonacion de proteinas en levaduras - Google Patents

Metodo de clonacion de proteinas en levaduras

Info

Publication number
MX9206979A
MX9206979A MX9206979A MX9206979A MX9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A MX 9206979 A MX9206979 A MX 9206979A
Authority
MX
Mexico
Prior art keywords
interest
proteins
protein
host cells
yeast
Prior art date
Application number
MX9206979A
Other languages
English (en)
Inventor
Henrik Dalboge
Hans Peter Heldt-Hansen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of MX9206979A publication Critical patent/MX9206979A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Esta invención describe un método de selección para una secuencia de ADN que codifica para una proteína de interés, el método comprende: (a) Clonar, en vectores adecuados, una biblioteca de ADN de un organismo que se sospecha produce una o más proteínas de interes. (b) Transformar células huésped de levadura adecuadas con los vetores. (c) Cultivar las células huesped bajo condiciones adecuadas,para expresar cualquier proteína de interés codificada por un clon en la biblioteca ADN y (d) Seleccionar clones positivos, determinando cualquier actividad de una proteína expresada en el paso (c).
MX9206979A 1991-12-04 1991-12-04 Metodo de clonacion de proteinas en levaduras MX9206979A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK9100379 1991-12-04
DK9100378 1991-12-04

Publications (1)

Publication Number Publication Date
MX9206979A true MX9206979A (es) 1993-07-01

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9206979A MX9206979A (es) 1991-12-04 1991-12-04 Metodo de clonacion de proteinas en levaduras

Country Status (7)

Country Link
EP (1) EP0618974A1 (es)
JP (1) JPH08504560A (es)
BR (1) BR9206866A (es)
CA (1) CA2124939A1 (es)
FI (1) FI942644A0 (es)
MX (1) MX9206979A (es)
WO (1) WO1993011249A1 (es)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0759073A1 (en) * 1994-05-11 1997-02-26 Novo Nordisk A/S AN ENZYME WITH ENDO-1,3(4)-$g(b)-GLUCANASE ACTIVITY
EP1995303A3 (en) * 1994-10-06 2008-12-31 Novozymes A/S Enzyme preparation with endoglucanase activity
AU3697995A (en) * 1994-10-26 1996-05-23 Novo Nordisk A/S An enzyme with lipolytic activity
CN1182451A (zh) 1995-03-17 1998-05-20 诺沃挪第克公司 新的内切葡聚糖酶
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
EP0796328A2 (en) * 1995-10-13 1997-09-24 Gist-Brocades B.V. Protein detection
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
EP0877799A1 (en) * 1996-01-29 1998-11-18 Novo Nordisk A/S Process for desizing cellulosic fabric
EP0882123B1 (en) * 1996-01-29 2004-09-22 Novozymes A/S Process for removal or bleaching of soiling or stains from cellulosic fabric
WO1997043409A2 (en) * 1996-05-10 1997-11-20 Novo Nordisk A/S Method of providing novel dna sequences
EP0922109A1 (en) 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
EP1616580B1 (en) 1996-07-05 2007-09-19 Novozymes A/S Alpha-amylase transcription factor
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
AU4699797A (en) * 1996-10-28 1998-05-22 Novo Nordisk A/S Extracellular expression of cellulose binding domains (cbd) using bacillus
DK0869167T4 (da) 1996-12-09 2010-03-08 Novozymes As Reduktion af phosphor-indeholdende bestanddele i spiseolier; som omfatter en stor mængde ikke-hydrerbart phosphor, ved anvendelse af en phospholipase, en phospholipase fra en trådsvamp, der har en phospholipase A og/eller B aktivitet
EP0977869B2 (en) 1997-04-09 2008-11-12 Danisco A/S Lipase and use of same for improving doughs and baked products
DK1042461T3 (da) * 1997-12-22 2007-09-24 Dsm Ip Assets Bv Ekspressionskloning i filiforme svampe
CN1167810C (zh) * 1998-03-06 2004-09-22 诺沃奇梅兹有限公司 荧光偏振筛选法
CN100497614C (zh) 1998-06-10 2009-06-10 诺沃奇梅兹有限公司 甘露聚糖酶
CA2343878A1 (en) * 1998-10-28 2000-05-04 Novozymes A/S Method for generating a gene library
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (en) * 1999-06-11 2000-12-13 The Procter & Gamble Company Non-aqueous liquid detergent compositions comprising a borate-releasing compound and a mannanase
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
DK1272669T3 (da) * 2000-04-13 2009-07-13 Dyadic Internat Usa Inc High-throughput screening af udtrykte DNA-biblioteker i trådsvampe
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
WO2005074647A2 (en) 2004-01-30 2005-08-18 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EP2267108A1 (en) 2004-07-16 2010-12-29 Danisco A/S Enzymatic oil-degumming method
BRPI0708949B1 (pt) 2006-03-20 2017-04-04 Novozymes As métodos para degradar ou converter um material celulósico, para produzir uma susbtância, e para produzir um polipeptídeo tendo atividade endoglucanase, e, célula microbiana recombinante
WO2008073914A2 (en) 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0406314B1 (en) * 1988-03-24 1993-12-01 Novo Nordisk A/S A cellulase preparation
AU639570B2 (en) * 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
MY106504A (en) * 1990-07-13 1995-06-30 Transkaryotic Therapies Inc Library screening method.

Also Published As

Publication number Publication date
CA2124939A1 (en) 1993-06-10
FI942644A7 (fi) 1994-06-03
JPH08504560A (ja) 1996-05-21
FI942644L (fi) 1994-06-03
WO1993011249A1 (en) 1993-06-10
FI942644A0 (fi) 1994-06-03
BR9206866A (pt) 1995-11-21
EP0618974A1 (en) 1994-10-12

Similar Documents

Publication Publication Date Title
MX9206979A (es) Metodo de clonacion de proteinas en levaduras
Lirazan et al. The spasmodic peptide defines a new conotoxin superfamily
DE69838106D1 (de) Expressionsklonierung in filamentösen pilzen
MY202182A (en) Flt3l-fc fusion proteins and methods of use
WO2019210268A3 (en) Sequencing-based proteomics
Files et al. Translational reinitiation: reinitiation of lac repressor fragments at three internal sites early in the lac i gene of Escherichia coli
ES8705467A1 (es) Un procedimiento para producir la proteina apoacuorina
DE3583361D1 (de) Verfahren zur herstellung von thrombin-inhibitoren.
PH12021553042A1 (en) Sorting with counter selection using sequence similar peptides
EP1009814A4 (en) KDR, A HUMAN TYROSIN KINASE RECEPTOR
BR9813608B1 (pt) célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
ES2006941A6 (es) Un metodo de producir un peptido o una proteina relacionados con un epitope de virus asociado a linfoadenopatiasnvirus de leucemia de celulas t humanas (lavnhtlv-iii).
AR109451A1 (es) Composiciones y métodos para preparar anticuerpos basados en el uso de loci mejoradores de expresión
NO962188D0 (no) Nye apoptose-modulerende proteiner, DNA som koder for proteinene og fremgangsmåter for anvendelse derav
Reichert et al. An application of information theory to genetic mutations and the matching of polypeptide sequences
DK4387A (da) Human-praepo-insulinlignende-vaekstfaktor i samt gener og syntetiske dna-sekvenser, som koder for denne
WO2003027634A3 (en) Identification of modulatory molecules using inducible promoters
DK0981631T3 (da) Polypeptider, som har aminopeptidase-aktivitet, og nukleinsyrer, som koder for samme
ATE384787T1 (de) Tripeptidyl-aminopeptidase
Dwulit-Smith et al. acI Actinobacteria assemble a functional actinorhodopsin with natively synthesized retinal
ATE419265T1 (de) Benzoat- und antranilatinduzierbare promotoren
JPS55111789A (en) Vector suitable for inserting extrinsic dna segment corresponding to any possible translation stage into genom and producing means
AU8515391A (en) Test to determine an organism's species and/or population identity by direct nucleotide sequence analysis of defined segments of its genome
Gerasimov et al. Evolutionary divergent kinetoplast genome structure and RNA editing patterns in the trypanosomatid Vickermania
DK0432254T3 (da) Nucleinsyre, som koder for humant testikulært angiotensinomdannende enzym (ACE), og dens anvendelser, især til påvisning in vitro af dette enzym i organismen