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BR9809946A - Produção de ácidos graxos poliinsaturados por expressão de genes de sìntese semelhantes a policetìdeo em plantas - Google Patents

Produção de ácidos graxos poliinsaturados por expressão de genes de sìntese semelhantes a policetìdeo em plantas

Info

Publication number
BR9809946A
BR9809946A BR9809946-9A BR9809946A BR9809946A BR 9809946 A BR9809946 A BR 9809946A BR 9809946 A BR9809946 A BR 9809946A BR 9809946 A BR9809946 A BR 9809946A
Authority
BR
Brazil
Prior art keywords
production
plants
genes
acid
polyunsaturated fatty
Prior art date
Application number
BR9809946-9A
Other languages
English (en)
Inventor
Daniel Facciotti
James George Metz
Michael Lassner
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of BR9809946A publication Critical patent/BR9809946A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Patente de Invenção:<B>"PRODUçãO DE áCIDOS GRAXOS POLIINSATURADOS POR EXPRESSãO DE GENES DE SìNTESE SEMELHANTES A POLICETìDEO EM PLANTAS"<D>. A presente invenção refere-se às composições e processos para preparação de ácidos graxos de cadeia longa, poliinsaturados, em plantas, partes de plantas e células de plantas, tais como, folhas, razíes, frutos e sementes. Seq³ências de ácido nucléico e constructos codificando genes semelhantes a PKS necessários para podução de ácido graxo de cadeia longa poliinsaturado, incluindo os genes responsáveis pela produção de acido eicosapentenóico de Shewanella putrefaciens e novos genes associados à produção de ácido docosahexenóico em Vibrio marinus são usados para gerar plantas transgênicas, partes de plantas e células que contêm e expressam um ou mais transgenes codificando um ou mais dos genes semelhanets a PKS associados a tal produção de ácido graxo poliinsaturado de cadeia longa. A expressão dos genes semelhantes a PKS no sistema de planta permite uma produção em grande escala de ácidos graxos de cadeia longa poliinsaturados, tais como, ácido eicosapentenóico e ácido docosahexenóico para modificação do perfil de ácido graxo das plantas, partes de plantas e tecidos. A manipulação de perfis de ácido graxo permite a produção de quantidades comerciais de novos óleos de planta e produtos.
BR9809946-9A 1997-06-04 1998-06-04 Produção de ácidos graxos poliinsaturados por expressão de genes de sìntese semelhantes a policetìdeo em plantas BR9809946A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4865097P 1997-06-04 1997-06-04
PCT/US1998/011639 WO1998055625A1 (en) 1997-06-04 1998-06-04 Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants

Publications (1)

Publication Number Publication Date
BR9809946A true BR9809946A (pt) 2000-08-01

Family

ID=21955694

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9809946-9A BR9809946A (pt) 1997-06-04 1998-06-04 Produção de ácidos graxos poliinsaturados por expressão de genes de sìntese semelhantes a policetìdeo em plantas

Country Status (9)

Country Link
US (1) US6140486A (pt)
EP (1) EP1003869A1 (pt)
JP (1) JP2002510205A (pt)
BR (1) BR9809946A (pt)
CA (1) CA2283422A1 (pt)
IL (1) IL133272A0 (pt)
IN (1) IN1998CH01219A (pt)
MX (1) MX9911200A (pt)
WO (1) WO1998055625A1 (pt)

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Also Published As

Publication number Publication date
IL133272A0 (en) 2001-04-30
MX9911200A (es) 2001-06-01
US6140486A (en) 2000-10-31
WO1998055625A1 (en) 1998-12-10
IN1998CH01219A (en) 2005-03-04
EP1003869A1 (en) 2000-05-31
JP2002510205A (ja) 2002-04-02
CA2283422A1 (en) 1998-12-10

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