[go: up one dir, main page]

BR9808648A - Intron sense inhibition of starch branching enzyme expression - Google Patents

Intron sense inhibition of starch branching enzyme expression

Info

Publication number
BR9808648A
BR9808648A BR9808648-0A BR9808648A BR9808648A BR 9808648 A BR9808648 A BR 9808648A BR 9808648 A BR9808648 A BR 9808648A BR 9808648 A BR9808648 A BR 9808648A
Authority
BR
Brazil
Prior art keywords
intron
enzyme expression
branching enzyme
starch branching
nucleotide sequence
Prior art date
Application number
BR9808648-0A
Other languages
Portuguese (pt)
Inventor
Peter Poulsen
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9703672.7A external-priority patent/GB9703672D0/en
Priority claimed from GBGB9706075.0A external-priority patent/GB9706075D0/en
Application filed by Danisco filed Critical Danisco
Publication of BR9808648A publication Critical patent/BR9808648A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

"INIBIçãO DE SENSO INTRON DA EXPRESSãO DE ENZIMA DE RAMIFICAçãO DE AMIDO" é descrito um método de inibição de expressão de gene. O método, que afeta a atividade enzimática em uma planta, compreende expressar em uma planta (ou célula, ou tecido ou órgão da mesma) uma seq³ência de nucleotídio onde a sequência de nucleotídio codifica, parcial ou completamente por uma classe de A SBE intron, com uma orientação de senso; e onde a seq³ência de nucleotídio não contém a seq³ência que tem senso a uma seq³ência exon normalmente associada com o intron."INTRON SENSE INHIBITION OF STARCH BRANCH ENZYME EXPRESSION" describes a method of inhibiting gene expression. The method, which affects enzyme activity in a plant, comprises expressing in a plant (or cell, or tissue or organ thereof) a nucleotide sequence where the nucleotide sequence encodes, partially or completely by a class of A SBE intron, with a sense orientation; and where the nucleotide sequence does not contain the sequence that makes sense for an exon sequence normally associated with the intron.

BR9808648-0A 1997-02-21 1998-02-23 Intron sense inhibition of starch branching enzyme expression BR9808648A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9703672.7A GB9703672D0 (en) 1997-02-21 1997-02-21 Inhibition of gene expression
GBGB9706075.0A GB9706075D0 (en) 1997-03-24 1997-03-24 Inhibition of gene expession
PCT/IB1998/000295 WO1998037214A1 (en) 1997-02-21 1998-02-23 Sense intron inhibition of starch branching enzyme expression

Publications (1)

Publication Number Publication Date
BR9808648A true BR9808648A (en) 2000-05-23

Family

ID=26311046

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9808648-0A BR9808648A (en) 1997-02-21 1998-02-23 Intron sense inhibition of starch branching enzyme expression

Country Status (8)

Country Link
EP (1) EP1007708A1 (en)
JP (1) JP2001512972A (en)
CN (1) CN1248293A (en)
AU (1) AU738311B2 (en)
BR (1) BR9808648A (en)
CA (1) CA2280210A1 (en)
NZ (1) NZ336607A (en)
WO (1) WO1998037214A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2826021B1 (en) * 2001-06-13 2004-12-31 Centre Nat Rech Scient METHOD FOR DETECTION AND IDENTIFICATION OF THE PRESENCE OF BIOLOGICAL MATERIALS FROM FISH, AND OLIGONUCLEOTIDES FOR IMPLEMENTING SAME
US7714186B2 (en) 2002-12-19 2010-05-11 Bayer Cropscience Ag Plant cells and plants which synthesize a starch with an increased final viscosity
FR2858631A1 (en) * 2004-09-24 2005-02-11 Centre Nat Rech Scient Method for detecting material from gadiform fish, useful for detecting adulteration of foods, based on amplification of mitochondrial DNA, also new oligonucleotides and amplicons
EP1707632A1 (en) 2005-04-01 2006-10-04 Bayer CropScience GmbH Phosphorylated waxy potato starch
WO2013053730A1 (en) 2011-10-12 2013-04-18 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
CA2851563A1 (en) 2011-10-12 2013-04-18 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
CN104017829A (en) * 2011-12-06 2014-09-03 中国科学院上海生命科学研究院 Method for increasing amylose content of plants
WO2017202715A1 (en) 2016-05-26 2017-11-30 Nunhems B.V. Seedless fruit producing plants
US11291176B2 (en) 2018-06-15 2022-04-05 Nunhems B.V. Seedless watermelon plants comprising modifications in an ABC transporter gene

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034323A (en) * 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
EP0575518A1 (en) * 1991-03-06 1993-12-29 Board Of Regents, The University Of Texas System Methods and compositions for the selective inhibition of gene expression
ATE366318T1 (en) * 1995-05-05 2007-07-15 Nat Starch Chem Invest IMPROVEMENTS IN OR RELATING TO PLANT STARCH COMPOUNDS
GB9514435D0 (en) * 1995-07-14 1995-09-13 Danisco Inhibition of gene expression
GB9514437D0 (en) * 1995-07-14 1995-09-13 Danisco Inhibition of gene expression

Also Published As

Publication number Publication date
AU738311B2 (en) 2001-09-13
NZ336607A (en) 2001-06-29
CN1248293A (en) 2000-03-22
AU6225998A (en) 1998-09-09
JP2001512972A (en) 2001-08-28
EP1007708A1 (en) 2000-06-14
CA2280210A1 (en) 1998-08-27
WO1998037214A1 (en) 1998-08-27

Similar Documents

Publication Publication Date Title
BR9808647A (en) Inhibition of antisense intron of starch branching expression enzyme
MX9800499A (en) Inhibition of gene expression.
MX9800500A (en) INHIBITION OF THE EXPRESSION OF GENES.
Anand-Srivastava et al. Pertussis toxin attenuates atrial natriuretic factor-mediated inhibition of adenylate cyclase. Involvement of inhibitory guanine nucleotide regulatory protein.
Geiman et al. Chromatin remodeling, histone modifications, and DNA methylation—how does it all fit together?
Dupret et al. Structure and regulation of the drug-metabolizing enzymes arylamine N-acetyltransferases
Lu et al. Regulation of the expression of the tissue transglutaminase gene by DNA methylation
Hepburn et al. Enzymatic methylation of cytosine in DNA is prevented by adjacent O6-methylguanine residues
AR055400A2 (en) CELL OF A FERTIL TRANSGENIC CORN PLANT AND PROGENIE CELL
TR200002446T2 (en) Inhibitors of phospholipase enzymes
BR9808648A (en) Intron sense inhibition of starch branching enzyme expression
Desseyn et al. Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
BR9809364A (en) Regulation metabolism by modifying the level of trehalose-6-phosphate by inhibiting endogenous trehalase levels
DE69930950D1 (en) ZINCFINGER BINDING DOMAIN FOR GNN
AU3950785A (en) Herbicide resistance genetically induced in plants
Guibert et al. Pheomelanin coat colour dilution in French cattle breeds is not correlated with the TYR, TYRP1 and DCT transcription levels
GB2297691B (en) Method for improving breeding activity of boars by using feed for the breeding boars
DE69736858D1 (en) IMMORTAL BIRDS
DK1112362T3 (en) Gene change in the G $ g (b) 3 subunit of the human G protein
BR0012676A (en) Methods for treating therapy resistant tumors
Lu et al. A novel mutation of keratin 9 in epidermolytic palmoplantar keratoderma combined with knuckle pads
Chakrabarti et al. Expression of the murine homologue of FMR2 in mouse brain and during development
Martini P0‐deficient knockout mice as tools to understand pathomechanisms in Charcot‐Marie‐Tooth 1B and P0‐related Dejerine‐Sottas syndrome
WO2000018907A3 (en) Antisense-sequences for inhibiting the expression of the adhesion molecule icam-1
Kempken et al. Mutations at specific atp6 codons which cause human mitochondrial diseases also lead to male sterility in a plant

Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law

Free format text: REFERENTE A 7O,8O E 9O ANUIDADES

B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1909 DE 07/08/2007.