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BR9713540A - Procedimento para a expressão do gene do vìrus da dengue - Google Patents

Procedimento para a expressão do gene do vìrus da dengue

Info

Publication number
BR9713540A
BR9713540A BR9713540-2A BR9713540A BR9713540A BR 9713540 A BR9713540 A BR 9713540A BR 9713540 A BR9713540 A BR 9713540A BR 9713540 A BR9713540 A BR 9713540A
Authority
BR
Brazil
Prior art keywords
dengue
virus
expression
procedure
serotype
Prior art date
Application number
BR9713540-2A
Other languages
English (en)
Inventor
Carlos Augusto Sariol Curbelo
Mayra Mune Jimenez
Maria Guadalupe Guzman Tirado
Gerardo Enrico Guilhen Nieto
Valexys Vazquez Villasuso
Rayner Rodriguez Diaz
Susana Vazquez Ramudo
Raysa Vazquez Campos
Gabriel Jesus Marquez Perera
Jose Garcia Suarez
Delfina Esperanza Ro Dominguez
Anabel Alvarez Acosta
Original Assignee
Ct Ingenieria Genetica Biotech
Inst De Medicina Tropical Pedr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech, Inst De Medicina Tropical Pedr filed Critical Ct Ingenieria Genetica Biotech
Publication of BR9713540A publication Critical patent/BR9713540A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24161—Methods of inactivation or attenuation
    • C12N2710/24162—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Patente de Invenção: <B>"PROCEDIMENTO PARA A EXPRESSãO DE GENES DO VìRUS DA DENGUE"<D>. A presente invenção está no campo da biotecnologia e refere-se a técnicas de DNA recombinantes, em particular a um procedimento para a expressão do gene que codifica a proteína do envelope do vírus da dengue sorotipo 2 e 4 em levedura metilotrófica de gênero Pichia. O objetivo técnico é desenvolver um sistema para a expressão eficiente do gene que codifica a proteína do envelope do vírus da dengue sorotipo 2 e 4 para obter um imunógeno para vacinação humana. A seq³ência genética que codifica a proteína E do vírus foi o ponto de partida do vírus da dengue sorotipo 2, retirada da cepa A 15 isolada durante as epidemias de febre de dengue e dengue hemorrágica em Cuba, 1981. Para o vírus da dengue sorotipo 4 o ponto de partida foi seq³ência genética que codifica a proteína E do vírus tirada da cepa 814669 isolada durante a epidemia de febre da dengue na República Dominicana em 1981.
BR9713540-2A 1996-11-25 1997-11-25 Procedimento para a expressão do gene do vìrus da dengue BR9713540A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU1996107A CU22666A1 (es) 1996-11-25 1996-11-25 Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados
PCT/CU1997/000006 WO1998023754A1 (es) 1996-11-25 1997-11-25 Procedimiento para la expresion de genes de los virus del dengue

Publications (1)

Publication Number Publication Date
BR9713540A true BR9713540A (pt) 2000-10-24

Family

ID=46093346

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9713540-2A BR9713540A (pt) 1996-11-25 1997-11-25 Procedimento para a expressão do gene do vìrus da dengue

Country Status (5)

Country Link
CN (1) CN1258317A (pt)
AU (1) AU5397398A (pt)
BR (1) BR9713540A (pt)
CU (1) CU22666A1 (pt)
WO (1) WO1998023754A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23245A1 (es) 2001-07-16 2007-10-17 Inst De Medicina Tropical Pedr CADENAS QUIMéRICAS CODIFICANTES PARA PROTEINAS INDUCTORAS DE EFECTOS CONTRA VIRUS. PREPARADOS UTILIZANDO PROTEINAS QUIMéRICAS
JP4500049B2 (ja) * 2001-12-04 2010-07-14 バヴァリアン・ノルディック・アクティーゼルスカブ フラビウイルスｎｓ１サブユニットワクチン
CU23578A1 (es) 2005-09-16 2010-09-30 Ct Ingenieria Genetica Biotech Proteína de la cápsida del virus dengue inductora de respuesta protectora y composición vacunal
CU23586A1 (es) * 2005-11-22 2010-10-30 Ct Ingenieria Genetica Biotech Métodos y proteínas para el tratamiento profiláctico y/o terapéutico de los cuatro serotipos del virus de dengue y otros flavivirus
CU23630A1 (es) 2006-10-30 2011-02-24 Ct Ingenieria Genetica Biotech Moléculas peptídicas quiméricas con propiedades antivirales contra los virus de la familia flaviviridae
CN102363751A (zh) * 2011-03-24 2012-02-29 中山大学 登革病毒样颗粒及其制备方法与应用
CN103103293B (zh) * 2013-02-01 2014-04-16 中华人民共和国北京出入境检验检疫局 登革2型病毒纳米磁分离实时荧光定量pcr检测试剂盒及核苷酸序列

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2224724C (en) * 1995-05-24 2007-12-04 Hawaii Biotechnology Group, Inc. Subunit vaccine against flavivirus infection

Also Published As

Publication number Publication date
CN1258317A (zh) 2000-06-28
CU22666A1 (es) 2001-04-27
AU5397398A (en) 1998-06-22
WO1998023754A1 (es) 1998-06-04

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A E 9A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1894 DE 24/04/2007.